FastQCFastQC Report
Fri 27 May 2016
SRR939116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750619
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52920.30229307462103405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43570.24888339495915446No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28490.1627424356756096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.151432150570741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG80000.024.2129971
TACATGG80900.023.477652
ACATGGG82500.022.2185523
GTATCAA113600.021.7739771
GTACACG2005.4151496E-721.364411
GGTATCA88500.021.1364151
GAGTACT51550.021.03193912-13
AGTACTT52850.019.43959412-13
GTACTTT55800.019.38817414-15
TACCTGG13650.019.0848522
CATGGGG38300.018.6739834
TATCAAC132100.018.53732
ATCAACG131250.018.5129993
CAACGCA132450.018.345275
TCAACGC132500.018.3383484
AACGCAG135450.017.9307486
CATGGGA46550.017.908194
GTGTAGC8850.017.1666321
AGAGTAC111500.016.92051310-11
ACTTTTT65800.016.73065616-17
Produced by FastQC (version 0.11.5)