##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939116_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1750619 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25018807633186 34.0 31.0 34.0 31.0 34.0 2 32.34433934511164 34.0 31.0 34.0 31.0 34.0 3 32.41192115474583 34.0 31.0 34.0 30.0 34.0 4 35.827795197013174 37.0 35.0 37.0 35.0 37.0 5 35.86693849432687 37.0 35.0 37.0 35.0 37.0 6 35.950935069252644 37.0 37.0 37.0 35.0 37.0 7 35.88910265454676 37.0 37.0 37.0 35.0 37.0 8 35.88653099275171 37.0 37.0 37.0 35.0 37.0 9 37.589101911952284 39.0 38.0 39.0 35.0 39.0 10-11 37.50342707350943 39.0 38.0 39.0 35.0 39.0 12-13 37.41607225786993 39.0 38.0 39.0 35.0 39.0 14-15 38.7551588895128 40.5 38.0 41.0 35.0 41.0 16-17 38.676785182841044 40.0 38.0 41.0 35.0 41.0 18-19 38.57330207200995 40.0 38.0 41.0 34.0 41.0 20-21 38.563532670444 40.0 38.5 41.0 34.0 41.0 22-23 38.60879494624473 40.0 39.0 41.0 34.0 41.0 24-25 38.55896742809257 40.5 38.0 41.0 34.0 41.0 26-27 38.464678208108104 40.0 38.0 41.0 34.0 41.0 28-29 38.33335608718973 40.0 38.0 41.0 34.0 41.0 30-31 38.141903235369895 40.0 38.0 41.0 34.0 41.0 32-33 37.971541494751285 40.0 38.0 41.0 33.5 41.0 34-35 37.68937615780475 40.0 38.0 41.0 33.0 41.0 36-37 37.44571691498835 40.0 37.5 41.0 32.5 41.0 38-39 37.27671183735582 40.0 37.0 41.0 31.5 41.0 40-41 37.11212034143352 40.0 37.0 41.0 31.0 41.0 42-43 36.918994938361806 40.0 37.0 41.0 31.0 41.0 44-45 36.66511045521612 40.0 36.5 41.0 30.0 41.0 46-47 36.54761601467823 40.0 37.0 41.0 30.0 41.0 48-49 36.43422183810412 40.0 36.5 41.0 30.0 41.0 50-51 35.31493488874507 38.5 34.5 40.0 28.0 40.5 52-53 35.4537817766173 38.5 35.0 40.0 28.0 40.5 54-55 35.98052174687925 39.0 36.0 41.0 29.0 41.0 56-57 35.89570974609552 39.0 35.0 41.0 28.0 41.0 58-59 35.70396613997677 39.0 35.0 41.0 28.0 41.0 60-61 35.418549952902374 39.0 35.0 41.0 28.0 41.0 62-63 35.05245287524013 38.0 35.0 40.5 26.5 41.0 64-65 34.68072693144539 38.0 34.5 40.0 26.0 41.0 66-67 34.30639305297155 37.0 34.0 40.0 26.0 41.0 68-69 33.903070856651276 36.5 34.0 39.0 25.5 41.0 70-71 33.47509852229412 36.0 34.0 39.0 24.5 41.0 72-73 33.033682943004735 35.5 33.5 38.5 23.5 40.0 74-75 32.5883298993099 35.0 33.0 37.5 22.5 39.5 76-77 32.154768399063414 35.0 33.0 37.0 21.5 39.0 78-79 31.727605492685733 35.0 32.0 36.5 20.0 39.0 80-81 31.285984557462246 35.0 32.0 36.0 18.0 38.0 82-83 30.91374936522453 35.0 31.5 35.5 16.5 37.0 84-85 30.54719987615809 35.0 31.0 35.0 10.0 37.0 86-87 30.153199811038267 34.5 31.0 35.0 6.0 36.0 88-89 29.75206769719739 34.0 30.5 35.0 2.0 36.0 90-91 29.36486694135046 34.0 30.0 35.0 2.0 36.0 92-93 28.9765605765732 34.0 30.0 35.0 2.0 35.0 94-95 28.52321892998991 34.0 29.0 35.0 2.0 35.0 96-97 27.964188381366817 34.0 29.0 35.0 2.0 35.0 98-99 27.30272263696441 34.0 28.0 35.0 2.0 35.0 100-101 24.40282208750162 31.5 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09116163483753326 1101 2 0.15368598417637713 1101 3 0.16335421364002656 1101 4 0.17511412794450365 1101 5 0.15958111700242483 1101 6 0.1571699401919986 1101 7 0.05305413362071931 1101 8 0.16730551470958233 1101 9 0.05959888540041902 1101 10-11 0.10479327801100169 1101 12-13 0.19987280608316382 1101 14-15 0.25275929353698956 1101 16-17 0.28702873778705396 1101 18-19 0.2835892862521092 1101 20-21 0.2930108715802078 1101 22-23 0.3539026931398084 1101 24-25 0.36500236349101556 1101 26-27 0.253349027542761 1101 28-29 0.31361837930514724 1101 30-31 0.33414960148419226 1101 32-33 0.31193105202549276 1101 34-35 0.4317156927131549 1101 36-37 0.36100162972908123 1101 38-39 0.3108394983393268 1101 40-41 0.3688061728244776 1101 42-43 0.2887645398528207 1101 44-45 0.26481896007263117 1101 46-47 0.3552700644117124 1101 48-49 0.4765613292306625 1101 50-51 0.3950009204099274 1101 52-53 0.4660543453047765 1101 54-55 0.44199947786845684 1101 56-57 0.4231673453384701 1101 58-59 0.23747621800418273 1101 60-61 0.25385393625938235 1101 62-63 0.27499606187989656 1101 64-65 0.1594236488581302 1101 66-67 0.28635200176037756 1101 68-69 0.3371152503434729 1101 70-71 0.21369599906507375 1101 72-73 0.26887303710039845 1101 74-75 0.0830394426048926 1101 76-77 0.13616542845836221 1101 78-79 0.23339995556384352 1101 80-81 0.0018611518022702 1101 82-83 -0.08227031688394604 1101 84-85 0.023795293134693196 1101 86-87 0.0038445974494614177 1101 88-89 -0.15066496285760067 1101 90-91 -0.300826773948522 1101 92-93 -0.4571375014757635 1101 94-95 -0.5539473692855097 1101 96-97 -0.6107146928686262 1101 98-99 -0.581474343255195 1101 100-101 -0.42678483091303576 1102 1 0.19615955717426203 1102 2 0.2195877226692744 1102 3 0.3504331061040915 1102 4 0.11508554103835422 1102 5 0.255762785268125 1102 6 0.20645037252000265 1102 7 0.24690903657970154 1102 8 0.2733272572925358 1102 9 0.31712905502911326 1102 10-11 0.2974174646221499 1102 12-13 0.26493521212601934 1102 14-15 0.31630424394501944 1102 16-17 0.37899003015886734 1102 18-19 0.37799264880326433 1102 20-21 0.24534666547600636 1102 22-23 0.38388783404018767 1102 24-25 0.44052553215593804 1102 26-27 0.33606091112400804 1102 28-29 0.45245197261833425 1102 30-31 0.4089504830225792 1102 32-33 0.2937041798985902 1102 34-35 0.30722711333444863 1102 36-37 0.3222935664375868 1102 38-39 0.1893555898957402 1102 40-41 0.27350800095060634 1102 42-43 0.2320599966232777 1102 44-45 -0.008051543853170529 1102 46-47 0.07749083625817832 1102 48-49 0.35827963290017806 1102 50-51 0.3903745843419131 1102 52-53 0.3909617206830518 1102 54-55 0.3240556465703648 1102 56-57 0.3040334807324925 1102 58-59 0.2968186321401234 1102 60-61 0.38476369170427915 1102 62-63 0.3483473653688449 1102 64-65 0.2630753324180475 1102 66-67 0.362327252548468 1102 68-69 0.1891012136069108 1102 70-71 0.21340099263627366 1102 72-73 0.23710959048546698 1102 74-75 0.1566022108790861 1102 76-77 0.16044711173545778 1102 78-79 0.14146607529381328 1102 80-81 0.07068162646674025 1102 82-83 0.026698444197833737 1102 84-85 -0.08509384231807005 1102 86-87 -0.0626443256682947 1102 88-89 -0.2760714125335504 1102 90-91 -0.1549162896898153 1102 92-93 -0.10750114693721358 1102 94-95 -0.17193514696901246 1102 96-97 -0.3040154797699941 1102 98-99 -0.5241184362735929 1102 100-101 -0.9320510002458562 1103 1 0.21127948537421304 1103 2 0.2571897415742228 1103 3 0.2701063713005212 1103 4 0.22730612217643653 1103 5 0.2616209698823795 1103 6 0.29905666462516933 1103 7 0.2629993284002765 1103 8 0.20183237441765556 1103 9 0.3110569961009517 1103 10-11 0.319796689925667 1103 12-13 0.3644021979040133 1103 14-15 0.475011200132073 1103 16-17 0.39207472352996575 1103 18-19 0.36643314931201587 1103 20-21 0.3978379797786715 1103 22-23 0.4370338866232686 1103 24-25 0.5114844434285288 1103 26-27 0.4119642193610318 1103 28-29 0.3903553442890342 1103 30-31 0.3866130467884119 1103 32-33 0.4348329390932619 1103 34-35 0.37318805191266335 1103 36-37 0.3705715138956478 1103 38-39 0.3756330453143093 1103 40-41 0.4677825984254227 1103 42-43 0.4480694105219385 1103 44-45 0.43493445016520127 1103 46-47 0.4591403344035996 1103 48-49 0.48681273798662517 1103 50-51 0.5247416578126689 1103 52-53 0.5021217166712972 1103 54-55 0.5940871833880337 1103 56-57 0.484708414920064 1103 58-59 0.4908949994141807 1103 60-61 0.4201648196234018 1103 62-63 0.2828960391337958 1103 64-65 0.4284453949859284 1103 66-67 0.43113499111262854 1103 68-69 0.215737566411228 1103 70-71 0.09851619759987074 1103 72-73 0.3193416925485195 1103 74-75 0.14470346986588112 1103 76-77 0.20011608230138478 1103 78-79 0.08932810364523291 1103 80-81 -0.07349152000587722 1103 82-83 -0.004237468085413809 1103 84-85 0.007133776527897595 1103 86-87 -0.13151464068501184 1103 88-89 -0.14076783530349601 1103 90-91 -0.03279598517393367 1103 92-93 -0.2104983447122315 1103 94-95 -0.4686780426129218 1103 96-97 -0.2313121427306477 1103 98-99 -0.20998378717748167 1103 100-101 -0.24302082382311951 1104 1 0.16962576568367638 1104 2 0.210942587479245 1104 3 0.21484020785123192 1104 4 0.17866478409916198 1104 5 0.17039123068515494 1104 6 0.18884422869707862 1104 7 0.3187757346084936 1104 8 0.2109984169914796 1104 9 0.23095388124593086 1104 10-11 0.3481311992470708 1104 12-13 0.32196916049760205 1104 14-15 0.3748905329261518 1104 16-17 0.22336535030800064 1104 18-19 0.2565730932811192 1104 20-21 0.3448674922198478 1104 22-23 0.37581912565429576 1104 24-25 0.3801073461225286 1104 26-27 0.47205519668626295 1104 28-29 0.538501726063906 1104 30-31 0.4807719959465686 1104 32-33 0.3484757058502552 1104 34-35 0.31638512057842405 1104 36-37 0.2651893066646167 1104 38-39 0.3273705364527473 1104 40-41 0.40432922545004857 1104 42-43 0.45277593138274597 1104 44-45 0.6131695350404414 1104 46-47 0.7611028321856068 1104 48-49 0.7075243870995465 1104 50-51 0.7214471140363372 1104 52-53 0.657241554993071 1104 54-55 0.5325323300005564 1104 56-57 0.4339298886011207 1104 58-59 0.41910092756033634 1104 60-61 0.5428175988011716 1104 62-63 0.4174049856678508 1104 64-65 0.4217624644181015 1104 66-67 0.4246303331168946 1104 68-69 0.5825619154456021 1104 70-71 0.4671917906685721 1104 72-73 0.40099448755422173 1104 74-75 0.4627182048437035 1104 76-77 0.5084986835647811 1104 78-79 0.40593998342953697 1104 80-81 0.33447818032126264 1104 82-83 0.4862940924077179 1104 84-85 0.5188299983989424 1104 86-87 0.6897094171152887 1104 88-89 0.533475693360483 1104 90-91 0.7497579193702677 1104 92-93 0.4545614390781232 1104 94-95 0.2924898176746993 1104 96-97 0.3329382502604119 1104 98-99 0.3734935942816726 1104 100-101 0.06889619779201084 1105 1 0.06768944241705555 1105 2 0.17204317593270702 1105 3 0.2741492559925547 1105 4 0.2455981422902127 1105 5 0.24031148106050182 1105 6 0.29643978048871844 1105 7 0.18116211469746446 1105 8 0.2216393691476526 1105 9 0.3417433846800648 1105 10-11 0.24971250677597823 1105 12-13 0.18716908989111403 1105 14-15 0.2548549520192509 1105 16-17 0.23301265506422197 1105 18-19 0.2693447348619884 1105 20-21 0.17423066538621157 1105 22-23 0.21636271623190595 1105 24-25 0.1869418949054591 1105 26-27 0.29805266199289804 1105 28-29 0.16533853584092384 1105 30-31 0.30606716475755036 1105 32-33 0.31409312581801174 1105 34-35 0.1515554785493194 1105 36-37 0.21889227947517043 1105 38-39 0.1993506714380615 1105 40-41 -0.013773602180883415 1105 42-43 -0.0762754625994404 1105 44-45 -0.15142111594735752 1105 46-47 -0.028354985399765553 1105 48-49 -0.11704032195613223 1105 50-51 -0.15757343919321443 1105 52-53 -0.034116290953896566 1105 54-55 -0.04130677845377306 1105 56-57 -0.08135502640335801 1105 58-59 -0.08040482706626761 1105 60-61 -0.09567860213376633 1105 62-63 -0.231074297478024 1105 64-65 -0.15730687868379079 1105 66-67 -0.08812306184505303 1105 68-69 -0.24763068555042622 1105 70-71 -0.2781778322809174 1105 72-73 -0.14800462589808916 1105 74-75 -0.25117976878993176 1105 76-77 -0.3844130999827158 1105 78-79 -0.3992222230626865 1105 80-81 -0.25322677185078035 1105 82-83 -0.18240621282648206 1105 84-85 -0.3193879415974479 1105 86-87 -0.48976829325137317 1105 88-89 -0.41356230522341875 1105 90-91 -0.3263312748630085 1105 92-93 -0.4581173827456233 1105 94-95 -0.38637643262528343 1105 96-97 -0.3220860157594281 1105 98-99 -0.35983623517194374 1105 100-101 -0.6208646723337132 1106 1 0.15728257764047981 1106 2 0.14651441299139378 1106 3 0.19019263339962578 1106 4 0.23125651989417406 1106 5 0.17818570308186565 1106 6 0.09755290641108871 1106 7 0.0980549632009513 1106 8 0.08298018332725832 1106 9 0.1376014161198711 1106 10-11 0.21251017326200383 1106 12-13 0.20203970962997175 1106 14-15 0.23491732157480527 1106 16-17 0.26089855965717845 1106 18-19 0.2162199751235434 1106 20-21 0.17983773718620455 1106 22-23 0.32552425263070717 1106 24-25 0.17067649238179428 1106 26-27 0.2546979449905322 1106 28-29 0.27759060935674285 1106 30-31 0.16162369959054956 1106 32-33 0.05710899198786734 1106 34-35 0.14992048109023415 1106 36-37 0.21565397564204858 1106 38-39 0.263417293974733 1106 40-41 0.4223626732352699 1106 42-43 0.4771873978866523 1106 44-45 0.35553385833411255 1106 46-47 0.19500715744263886 1106 48-49 0.15798672797889424 1106 50-51 0.25494378931826134 1106 52-53 0.060993905887116284 1106 54-55 0.1966246375376599 1106 56-57 0.2654611446759958 1106 58-59 0.3141122401769607 1106 60-61 0.5092305157101791 1106 62-63 0.3029349248152471 1106 64-65 0.11689976594288254 1106 66-67 0.15176870091060835 1106 68-69 0.3074144789338078 1106 70-71 0.10713531876930205 1106 72-73 0.21672882207320043 1106 74-75 0.20474805804548168 1106 76-77 0.07244726984662009 1106 78-79 0.07438641641465082 1106 80-81 0.10649160879102837 1106 82-83 0.19106797394089625 1106 84-85 0.2911680025437313 1106 86-87 0.26778684267338093 1106 88-89 0.3611898386128516 1106 90-91 0.39756324070406635 1106 92-93 0.2736849023878811 1106 94-95 0.30907674051493217 1106 96-97 0.21082620743582225 1106 98-99 0.22749351900650083 1106 100-101 0.23721458538231843 1107 1 0.11427833778584073 1107 2 0.15014857410587013 1107 3 0.05330589808769304 1107 4 0.1064836549638315 1107 5 0.1406327048989482 1107 6 0.09331305177753535 1107 7 0.15741292807072682 1107 8 0.15869187321217737 1107 9 0.09096301515076277 1107 10-11 0.170717320445533 1107 12-13 0.16933225959968468 1107 14-15 0.1973643233918878 1107 16-17 0.2418192138061812 1107 18-19 0.28920604417260165 1107 20-21 0.3121817711050454 1107 22-23 0.33218688134058283 1107 24-25 0.2763700114611467 1107 26-27 0.3667512460202218 1107 28-29 0.31393222050149916 1107 30-31 0.42025483223743265 1107 32-33 0.23609142687580942 1107 34-35 0.2640937094366933 1107 36-37 0.52092350925804 1107 38-39 0.40514957743930324 1107 40-41 0.510793927020849 1107 42-43 0.4381201659060423 1107 44-45 0.547841550641806 1107 46-47 0.6674481023245278 1107 48-49 0.6676778242841692 1107 50-51 0.47844469785854216 1107 52-53 0.5734106230282521 1107 54-55 0.5276361457145242 1107 56-57 0.5413545426166451 1107 58-59 0.6605689330903459 1107 60-61 0.540120885183228 1107 62-63 0.4316447976196045 1107 64-65 0.5181717223329514 1107 66-67 0.42584501829401233 1107 68-69 0.5412121772984406 1107 70-71 0.3930226597747506 1107 72-73 0.5344150728303205 1107 74-75 0.4558080217038665 1107 76-77 0.43798330861100254 1107 78-79 0.5180569191402746 1107 80-81 0.4487084470708602 1107 82-83 0.38125573893181297 1107 84-85 0.4038269970924908 1107 86-87 0.3032199135395217 1107 88-89 0.35422436314344097 1107 90-91 0.37000418558595527 1107 92-93 0.336677028372133 1107 94-95 0.41265033227749726 1107 96-97 0.2653386241529603 1107 98-99 0.4388805571651915 1107 100-101 0.3344283951945535 1108 1 0.17173587301937232 1108 2 0.1544403453267762 1108 3 0.1603617861173845 1108 4 0.11427644067723719 1108 5 0.19442605922259304 1108 6 0.17923500384122093 1108 7 0.12115254696059452 1108 8 0.16176519391365218 1108 9 0.21838998316908942 1108 10-11 0.20047205871706097 1108 12-13 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-1.3581500112850335 2316 96-97 -1.4898786478267851 2316 98-99 -1.3927507119087146 2316 100-101 -1.551104155921788 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5105.0 3 1318.0 4 1244.0 5 1442.0 6 1633.0 7 1951.0 8 2308.0 9 2952.0 10 3527.0 11 4450.0 12 5786.0 13 9923.0 14 7960.0 15 7779.0 16 9694.0 17 14182.0 18 12146.0 19 8400.0 20 8190.0 21 8624.0 22 9237.0 23 10433.0 24 11440.0 25 13092.0 26 15018.0 27 17553.0 28 20967.0 29 24946.0 30 30502.0 31 37395.0 32 48043.0 33 62750.0 34 87893.0 35 133059.0 36 224350.0 37 389069.0 38 417887.0 39 78328.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92892738477136 18.62525045962998 14.212860005252914 23.23296215034574 2 15.391654203923832 20.187470153756408 40.2248154894498 24.196060152869958 3 19.713416412643763 24.219086890130566 29.529662058986695 26.537834638238976 4 13.034938331839511 16.295174365629876 36.71341850897067 33.95646879355995 5 14.217483569471534 37.08666281524021 33.64154278229336 15.054310832994904 6 32.398105305761256 35.09111613405545 17.588575126364397 14.922203433818899 7 29.001778047121107 32.435352836585466 20.458233044233925 18.104636072059506 8 26.296288461879286 34.81783043014189 19.847618895165528 19.038262212813297 9 26.61971476692321 17.271202390167655 20.460379403670093 35.64870343923904 10-11 25.331083945297795 26.37695219545437 26.986539402069443 21.305424457178397 12-13 26.28372244061626 23.65596192854513 27.598317336827638 22.461998294010975 14-15 23.09798259772949 24.96211957722561 26.121384852804436 25.81851297224046 16-17 22.61920517212943 28.44259574902321 26.529716219472494 22.40848285937487 18-19 22.436275297347642 26.970172899870615 28.766436808158957 21.827114994622786 20-21 23.314254710195886 26.112671933441504 28.495270079436136 22.07780327692647 22-23 22.925811071089278 26.10288200083881 28.44631774197191 22.5249891861 24-25 22.933696197927805 26.2227522734317 28.5305527947398 22.312998733900695 26-27 22.6499304324852 26.413953304842963 28.538607540283678 22.39750872238816 28-29 22.288123017698577 26.702631269705073 28.73962155394419 22.269624158652164 30-31 22.511482036848296 26.418047632048786 28.50361149904727 22.566858832055644 32-33 22.297724385940988 26.531020068252126 28.470262124872747 22.70099342093414 34-35 23.04743519345383 26.452866872170645 28.182608127456792 22.317089806918737 36-37 22.876354089142477 26.242626588384464 28.26764450508559 22.61337481738747 38-39 22.676147511924018 26.516865866804075 28.519383438854145 22.287603182417758 40-41 22.750703981210332 26.31819261936112 28.478723294345077 22.45238010508347 42-43 22.559881588926864 26.427963248013896 28.112783331964962 22.899371831094275 44-45 22.66964638950727 26.595547986304148 27.939782343189883 22.7950232809987 46-47 22.711473673180247 26.758401313721297 27.66422592790897 22.865899085189486 48-49 22.9983292912652 26.721664059204937 27.564626912852397 22.71537973667747 50-51 22.66694956593242 26.97500963972549 27.610327124766325 22.747713669575763 52-53 22.99792768784743 26.979362435279725 27.3456776299961 22.677032246876745 54-55 22.51680402429575 26.683869026651312 27.586964216966773 23.212362732086163 56-57 22.741806749761288 27.158818942450726 27.375074656294046 22.724299651493943 58-59 22.99935201452314 26.621384209472065 27.524287976454424 22.85497579955037 60-61 22.934274360788 26.505463225411184 27.97534535604328 22.58491705775754 62-63 22.72540429506636 26.63189155953496 27.85699072083332 22.785713424565362 64-65 22.58184181995259 26.630519671438286 27.70795241551664 23.079686093092487 66-67 22.491091042226017 26.986087381010492 27.422705279329207 23.10011629743428 68-69 22.437023396233354 26.795528224592815 27.68219216480065 23.08525621437319 70-71 22.521326959775653 26.91741609576993 27.473632970955702 23.087623973498715 72-73 22.622609918138018 26.632695576168054 27.60582776680089 23.13886673889304 74-75 22.4720762754086 26.99583882877287 27.329427443399485 23.202657452419043 76-77 22.648135038132917 26.72080433101315 27.417950921844596 23.213109709009334 78-79 22.619645894818895 26.865590251359055 27.403501329853263 23.111262523968787 80-81 22.516302340206355 27.12337999126868 27.413275912892303 22.947041755632664 82-83 22.681422732463442 26.940537288136568 27.298913102764068 23.07912687663593 84-85 22.58568431338159 26.82182594370508 27.16442254173665 23.428067201176685 86-87 22.72657466171286 26.69554057192263 27.478167314597634 23.099717451766864 88-89 22.705567809292994 26.652036823796806 27.249259392913196 23.393135973997005 90-91 22.61501469286823 26.737229953581217 27.422169720574207 23.225585632976344 92-93 22.844923139832325 26.604627575932653 27.351606407942963 23.19884287629206 94-95 22.62697682538698 26.52233788280264 27.341179186608638 23.50950610520174 96-97 22.766934046985618 26.400932648587027 27.394829370964707 23.437303933462644 98-99 22.64073359932578 26.458505321523994 27.116929955162227 23.78383112398799 100-101 22.918159478204952 25.82965872031602 27.71433328740067 23.53784851407836 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1539.0 1 850.0 2 208.0 3 271.5 4 257.5 5 269.0 6 439.0 7 829.5 8 1361.0 9 1728.0 10 1747.0 11 1808.5 12 2025.0 13 2244.5 14 2473.0 15 2613.5 16 2755.0 17 2848.5 18 2819.5 19 2839.0 20 3044.5 21 3449.0 22 3983.5 23 4707.5 24 5686.0 25 6955.5 26 8708.0 27 10836.0 28 12815.0 29 14942.5 30 17395.0 31 20102.5 32 22986.5 33 25569.0 34 29350.0 35 32601.0 36 35344.0 37 39172.5 38 42021.5 39 43442.0 40 45232.0 41 49723.5 42 54548.5 43 58010.0 44 61293.0 45 64386.0 46 65559.5 47 68414.5 48 80012.0 49 82455.5 50 74761.0 51 71039.5 52 66821.0 53 65009.5 54 63244.5 55 57317.0 56 50497.5 57 44452.5 58 39096.5 59 34205.0 60 29322.5 61 24338.5 62 19864.5 63 15709.0 64 12022.0 65 9315.0 66 7076.0 67 5769.0 68 4355.5 69 2975.0 70 2311.0 71 1744.0 72 1393.0 73 1094.5 74 796.5 75 669.0 76 525.5 77 324.0 78 182.5 79 127.5 80 93.0 81 60.0 82 44.0 83 35.5 84 24.0 85 16.5 86 10.5 87 12.5 88 13.0 89 9.5 90 10.0 91 9.0 92 6.0 93 5.5 94 3.5 95 1.5 96 1.5 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38986210020569867 2 0.11847238034089656 3 0.11932922012156842 4 0.13195332622346725 5 0.1341811096532141 6 0.0750020421348106 7 0.08619808193558964 8 0.08294209076903655 9 0.08299921342108135 10-11 0.0801145194928194 12-13 0.08494138359060423 14-15 0.07794385871511735 16-17 0.07982890623259543 18-19 0.08979680901441146 20-21 0.08802600680102295 22-23 0.09782254162670462 24-25 0.11087506761893937 26-27 0.11184615270370081 28-29 0.10639093943342326 30-31 0.096622965933764 32-33 0.09496640902446507 34-35 0.34073661944717837 36-37 0.5270707104172867 38-39 0.5061923810949155 40-41 0.47897343739557263 42-43 0.5078203766781921 44-45 0.5181024540462544 46-47 0.5141895523811864 48-49 0.5222438463195018 50-51 0.52138700653883 52-53 0.5190164164789711 54-55 0.5139610617730072 56-57 0.4837431788413127 58-59 0.47388952136358625 60-61 0.4237358328682598 62-63 0.5003944319123692 64-65 0.52615674798457 66-67 0.5366101933087668 68-69 0.5515192054924573 70-71 0.5467494640467172 72-73 0.5332113955121017 74-75 0.5370957358511474 76-77 0.5111334904967899 78-79 0.48608520757514917 80-81 0.4920830860398522 82-83 0.5067921689413859 84-85 0.5012227103670187 86-87 0.502250918103825 88-89 0.5125901181239322 90-91 0.5311264187124669 92-93 0.5372385424812596 94-95 0.5322117491013179 96-97 0.5336112540764153 98-99 0.5336112540764153 100-101 0.5106193866283869 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1750619.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.349905582000424 #Duplication Level Percentage of deduplicated Percentage of total 1 78.20519016873887 44.85060272199563 2 12.8275274600705 14.713149773711223 3 4.043302196742398 6.956489976680144 4 1.6907999123575799 3.878688613270472 5 0.8574706796478722 2.45879312585696 6 0.5343912331589418 1.838837205930845 7 0.3497794604397471 1.4041873321519789 8 0.24989679155554448 1.1465245920764255 9 0.17118333143796535 0.883561310566164 >10 0.930665613682573 9.814666991446884 >50 0.07771308611875748 3.0985198240217344 >100 0.056982234531468154 6.004230139349461 >500 0.003998299229685091 1.5681969477641 >1k 9.995748074212727E-4 1.080184788957411 >5k 9.995748074212726E-5 0.3033666562204861 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5292 0.30229307462103405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4357 0.24888339495915446 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2849 0.1627424356756096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2651 0.151432150570741 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.712265204479101E-5 0.0 0.0 0.0 0.0 6 5.712265204479101E-5 0.0 0.0 0.0 0.0 7 5.712265204479101E-5 0.0 0.0 0.0 0.0 8 5.712265204479101E-5 0.0 0.0 0.0 0.0 9 5.712265204479101E-5 0.0 0.0 0.0 0.0 10-11 5.712265204479101E-5 0.0 0.0 0.0 0.0 12-13 1.1424530408958202E-4 0.0 0.0 5.712265204479101E-5 0.0 14-15 1.1424530408958202E-4 0.0 0.0 8.568397806718651E-5 2.8561326022395505E-5 16-17 1.1424530408958202E-4 0.0 0.0 1.1424530408958202E-4 5.712265204479101E-5 18-19 1.1424530408958202E-4 0.0 0.0 1.1424530408958202E-4 5.712265204479101E-5 20-21 1.1424530408958202E-4 0.0 0.0 1.4280663011197753E-4 5.712265204479101E-5 22-23 1.1424530408958202E-4 2.8561326022395505E-5 0.0 3.141745862463506E-4 5.712265204479101E-5 24-25 1.1424530408958202E-4 5.712265204479101E-5 0.0 6.569104985150966E-4 1.1424530408958202E-4 26-27 1.1424530408958202E-4 5.712265204479101E-5 0.0 0.0010853303888510293 1.1424530408958202E-4 28-29 1.7136795613437305E-4 5.712265204479101E-5 0.0 0.003084623210418715 1.1424530408958202E-4 30-31 1.7136795613437305E-4 5.712265204479101E-5 0.0 0.009425237587390518 1.1424530408958202E-4 32-33 2.2849060817916404E-4 5.712265204479101E-5 0.0 0.02116394258259507 1.1424530408958202E-4 34-35 2.2849060817916404E-4 5.712265204479101E-5 0.0 0.041271116102361505 1.1424530408958202E-4 36-37 2.2849060817916404E-4 5.712265204479101E-5 0.0 0.07380246644187 1.1424530408958202E-4 38-39 2.2849060817916404E-4 5.712265204479101E-5 0.0 0.12338492841674858 1.1424530408958202E-4 40-41 2.2849060817916404E-4 5.712265204479101E-5 0.0 0.17756576388123285 1.1424530408958202E-4 42-43 2.2849060817916404E-4 5.712265204479101E-5 0.0 0.233831576145352 1.1424530408958202E-4 44-45 2.2849060817916404E-4 5.712265204479101E-5 0.0 0.30600604700394546 1.1424530408958202E-4 46-47 2.2849060817916404E-4 5.712265204479101E-5 0.0 0.3902048361179674 1.1424530408958202E-4 48-49 2.8561326022395506E-4 5.712265204479101E-5 0.0 0.480801362261006 1.1424530408958202E-4 50-51 2.8561326022395506E-4 5.712265204479101E-5 0.0 0.5817942110761964 1.1424530408958202E-4 52-53 2.8561326022395506E-4 5.712265204479101E-5 0.0 0.6842722488445516 1.1424530408958202E-4 54-55 2.8561326022395506E-4 5.712265204479101E-5 0.0 0.7999741805612757 1.1424530408958202E-4 56-57 2.8561326022395506E-4 5.712265204479101E-5 0.0 0.9422381454788278 1.1424530408958202E-4 58-59 2.8561326022395506E-4 5.712265204479101E-5 0.0 1.0992397546239359 1.1424530408958202E-4 60-61 3.141745862463506E-4 5.712265204479101E-5 0.0 1.2694081350653683 1.1424530408958202E-4 62-63 3.427359122687461E-4 5.712265204479101E-5 0.0 1.4413758790462117 1.1424530408958202E-4 64-65 3.427359122687461E-4 5.712265204479101E-5 0.0 1.6233972097869382 1.1424530408958202E-4 66-67 3.427359122687461E-4 5.712265204479101E-5 0.0 1.841405811315883 1.1424530408958202E-4 68-69 3.998585643135371E-4 5.712265204479101E-5 0.0 2.091774395228202 1.1424530408958202E-4 70-71 4.2841989033593256E-4 5.712265204479101E-5 0.0 2.3697617814041774 1.1424530408958202E-4 72-73 4.569812163583281E-4 5.712265204479101E-5 0.0 2.6536042394147445 2.2849060817916404E-4 74-75 4.569812163583281E-4 5.712265204479101E-5 0.0 2.950527784743568 2.2849060817916404E-4 76-77 4.569812163583281E-4 5.712265204479101E-5 0.0 3.2665302958553517 2.2849060817916404E-4 78-79 4.569812163583281E-4 5.712265204479101E-5 0.0 3.627117036888095 2.2849060817916404E-4 80-81 4.569812163583281E-4 5.712265204479101E-5 0.0 4.02003519897819 2.2849060817916404E-4 82-83 4.569812163583281E-4 5.712265204479101E-5 0.0 4.428033741208109 2.2849060817916404E-4 84-85 5.141038684031191E-4 5.712265204479101E-5 0.0 4.849684597276735 2.2849060817916404E-4 86-87 5.997878464703056E-4 5.712265204479101E-5 0.0 5.295812509746552 2.2849060817916404E-4 88-89 6.283491724927012E-4 5.712265204479101E-5 0.0 5.77949856593582 2.2849060817916404E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8000 0.0 24.212997 1 TACATGG 8090 0.0 23.47765 2 ACATGGG 8250 0.0 22.218552 3 GTATCAA 11360 0.0 21.773977 1 GTACACG 200 5.4151496E-7 21.36441 1 GGTATCA 8850 0.0 21.136415 1 GAGTACT 5155 0.0 21.031939 12-13 AGTACTT 5285 0.0 19.439594 12-13 GTACTTT 5580 0.0 19.388174 14-15 TACCTGG 1365 0.0 19.084852 2 CATGGGG 3830 0.0 18.673983 4 TATCAAC 13210 0.0 18.5373 2 ATCAACG 13125 0.0 18.512999 3 CAACGCA 13245 0.0 18.34527 5 TCAACGC 13250 0.0 18.338348 4 AACGCAG 13545 0.0 17.930748 6 CATGGGA 4655 0.0 17.90819 4 GTGTAGC 885 0.0 17.166632 1 AGAGTAC 11150 0.0 16.920513 10-11 ACTTTTT 6580 0.0 16.730656 16-17 >>END_MODULE