FastQCFastQC Report
Fri 27 May 2016
SRR939116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750619
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54490.3112613309920662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.26670566239712923No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31560.18027908985336044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.16051465224586275No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA909.00014E-426.363367
GTACATG80650.024.6922281
TACATGG81000.023.9613652
ACATGGG83300.022.9009913
GTATCAA112900.022.196281
CATGGGG40300.021.6663634
GGTATCA87550.021.0048471
GAGTACT55200.020.93487212-13
AGTACTT56600.020.33320212-13
TACCTGG13800.019.6005822
GTACTTT60000.019.26008214-15
ATCAACG132950.018.6318243
TATCAAC133250.018.6254882
TCAACGC133050.018.546494
TCTATAC5650.018.4776823
CAACGCA133900.018.4287555
CATGGGA45350.018.2072834
AACGCAG137000.017.9771166
GTACCTG16400.017.7204341
ATGGGGA25200.017.701115
Produced by FastQC (version 0.11.5)