FastQCFastQC Report
Fri 27 May 2016
SRR939115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784485
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49600.6322619298010798No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26870.34251770269667364No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24510.31243427216581576No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22540.2873222560023455No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21880.2789090932267666No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18690.23824547314480202No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14520.18508958106273543No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13280.16928303281770843No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11690.1490149588583593No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11320.14429848881750448No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG10810.13779740849092081No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10500.13384577142966403No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10070.12836446840921112No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9620.12262822106222553No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9590.1222458045724265No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9420.12007877779689859No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8450.10771397796006296No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGCG650.00592329329.1491895
TGTACGC650.00592329329.1491892
GTCGGGA2002.3646862E-1128.4204582
TACATGG41050.026.6549992
GTACATG41900.026.3993231
ACATGGG42150.024.947973
GGTCGGG2801.8189894E-1223.7367291
CATGGGA26250.023.6386034
ATGGGAG9450.023.5584035
AAGACCG1252.2980885E-422.7363665
GAGTACG1050.002202740622.606411
TACCTGG6500.021.861892
CGGGAGT3251.8189894E-1221.861894
GTAAGTA2406.1136234E-921.7100722
CATGGGG15700.021.420944
ATGGGAT8950.021.1698025
CTTAGAC2257.35854E-821.052193
GTATAGC2509.53878E-920.8883231
CAGTGCG1150.003791392520.5866539
GTATTAG1656.3466374E-520.1402551
Produced by FastQC (version 0.11.5)