##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939115_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 784485 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.273681459811215 33.0 31.0 34.0 31.0 34.0 2 32.33302102653333 34.0 31.0 34.0 31.0 34.0 3 32.41593656985156 34.0 31.0 34.0 31.0 34.0 4 35.83144865739944 37.0 35.0 37.0 35.0 37.0 5 35.8895020299942 37.0 35.0 37.0 35.0 37.0 6 35.98979840277379 37.0 37.0 37.0 35.0 37.0 7 35.926443462908786 37.0 37.0 37.0 35.0 37.0 8 35.92228022205651 37.0 37.0 37.0 35.0 37.0 9 37.639557161704815 39.0 38.0 39.0 35.0 39.0 10-11 37.541612650337484 39.0 38.0 39.0 35.0 39.0 12-13 37.47836478708962 39.0 38.0 39.0 35.0 39.0 14-15 38.82068873209813 40.0 38.0 41.0 35.0 41.0 16-17 38.772127574140995 40.0 38.0 41.0 35.0 41.0 18-19 38.70683633211597 40.0 38.0 41.0 34.5 41.0 20-21 38.59062378503094 40.0 38.0 41.0 34.0 41.0 22-23 38.52289782468753 40.0 38.0 41.0 34.0 41.0 24-25 38.57510723595735 40.0 38.0 41.0 34.0 41.0 26-27 38.50391913165963 40.0 38.0 41.0 34.0 41.0 28-29 38.41007794922784 40.0 38.0 41.0 34.0 41.0 30-31 38.33058312141086 40.0 38.0 41.0 34.0 41.0 32-33 38.202281114361654 40.0 38.0 41.0 34.0 41.0 34-35 37.93620528117172 40.0 38.0 41.0 33.0 41.0 36-37 37.721596971261405 40.0 38.0 41.0 33.0 41.0 38-39 37.50582420313964 40.0 37.5 41.0 32.5 41.0 40-41 37.41565868053564 40.0 37.0 41.0 32.0 41.0 42-43 37.2837339146064 40.0 37.0 41.0 31.5 41.0 44-45 37.108401690280886 40.0 37.0 41.0 31.0 41.0 46-47 36.852433124916345 40.0 36.5 41.0 31.0 41.0 48-49 36.9606659145809 40.0 37.0 41.0 31.0 41.0 50-51 35.96446713448951 38.5 35.5 40.0 29.5 40.5 52-53 36.14059414775298 38.5 35.5 40.0 30.5 40.5 54-55 36.679081180647174 39.0 36.0 41.0 31.0 41.0 56-57 36.57792182132226 39.0 36.0 41.0 31.0 41.0 58-59 36.39920712314448 39.0 35.0 41.0 31.0 41.0 60-61 36.094597729720775 39.0 35.0 41.0 30.0 41.0 62-63 35.73938698636685 38.0 35.0 40.0 29.5 41.0 64-65 35.350692492526946 37.5 35.0 40.0 29.0 41.0 66-67 34.94484024551139 37.0 34.5 39.5 28.0 41.0 68-69 34.53619125923376 36.5 34.0 39.0 28.0 41.0 70-71 34.10880513967763 36.0 34.0 39.0 27.5 40.5 72-73 33.654879953090244 35.0 34.0 38.0 26.5 40.0 74-75 33.2108415074858 35.0 33.0 37.0 26.0 39.0 76-77 32.784458593854566 35.0 33.0 37.0 26.0 39.0 78-79 32.38525784431825 35.0 33.0 36.0 25.5 38.5 80-81 31.98645608265295 35.0 32.5 36.0 25.0 37.0 82-83 31.600871272235924 35.0 32.0 35.0 24.0 37.0 84-85 31.254551074909017 35.0 32.0 35.0 23.0 36.5 86-87 30.91771544388994 34.5 31.5 35.0 20.5 36.0 88-89 30.545821781168534 34.0 31.0 35.0 19.5 36.0 90-91 30.182780422825164 34.0 31.0 35.0 17.0 35.0 92-93 29.819556141927507 34.0 31.0 35.0 8.5 35.0 94-95 29.39548047445139 34.0 30.0 35.0 2.0 35.0 96-97 28.854124043162074 34.0 29.5 35.0 2.0 35.0 98-99 28.20735323173802 34.0 29.0 35.0 2.0 35.0 100-101 25.625163642389595 31.5 23.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29864087327781874 1101 2 0.3927691336044177 1101 3 0.35379017054644635 1101 4 0.1967298526079304 1101 5 0.303146793943867 1101 6 0.2691252245607103 1101 7 0.212944861832419 1101 8 0.33787571185748533 1101 9 0.34615077782997616 1101 10-11 0.49713840913830865 1101 12-13 0.4267348035638747 1101 14-15 0.5325378830950385 1101 16-17 0.5703422505076858 1101 18-19 0.5077024119256066 1101 20-21 0.48646266301769003 1101 22-23 0.597334232254866 1101 24-25 0.6244325005657245 1101 26-27 0.49062683586245015 1101 28-29 0.6044378235868066 1101 30-31 0.5215221518116806 1101 32-33 0.6022424611087089 1101 34-35 0.7338363792985945 1101 36-37 0.6510130630113409 1101 38-39 0.7078133676825757 1101 40-41 0.7365456638328567 1101 42-43 0.4817720828365637 1101 44-45 0.5194791821632307 1101 46-47 0.5592671652503469 1101 48-49 0.6795088732958163 1101 50-51 0.47312302013868646 1101 52-53 0.6849207138065907 1101 54-55 0.4947822225829981 1101 56-57 0.6125228175047539 1101 58-59 0.6102700405904784 1101 60-61 0.5075645044970543 1101 62-63 0.5127930945559029 1101 64-65 0.6013878849173437 1101 66-67 0.33282673095980186 1101 68-69 0.4499668969591184 1101 70-71 0.36816258852529415 1101 72-73 0.06847752502726223 1101 74-75 0.03931798030694722 1101 76-77 -0.2640605990932272 1101 78-79 -0.2535251146369788 1101 80-81 -0.22934262262518956 1101 82-83 -0.27564001974427654 1101 84-85 -0.22247504354778158 1101 86-87 -0.24710922366518062 1101 88-89 -0.1346955476907148 1101 90-91 -0.24323557856909517 1101 92-93 -0.389083636828218 1101 94-95 -0.4888488601731247 1101 96-97 -0.5276341915716181 1101 98-99 -0.6567681069013176 1101 100-101 -0.7050517130574612 1102 1 0.27511146151311294 1102 2 0.4532894955953637 1102 3 0.4719123424921463 1102 4 0.40632261278892656 1102 5 0.4907712283330028 1102 6 0.43457771324848693 1102 7 0.3011349070812912 1102 8 0.3438259381018298 1102 9 0.4377797371059984 1102 10-11 0.3503963276903477 1102 12-13 0.43409905695753537 1102 14-15 0.5732053039095248 1102 16-17 0.43494632290587276 1102 18-19 0.6788110092106692 1102 20-21 0.6864739752348754 1102 22-23 0.6732505218476277 1102 24-25 0.7690139485295262 1102 26-27 0.5814413155004559 1102 28-29 0.6171640679307018 1102 30-31 0.6727551834858829 1102 32-33 0.7821519633711489 1102 34-35 0.5326485964931607 1102 36-37 0.5577777688936862 1102 38-39 0.5715011504880039 1102 40-41 0.4055501887197366 1102 42-43 0.5507652955062454 1102 44-45 0.3767416256021363 1102 46-47 0.44671060416438024 1102 48-49 0.49644326243608816 1102 50-51 0.654197680772171 1102 52-53 0.5484501255712999 1102 54-55 0.6535378786915942 1102 56-57 0.5624436319843937 1102 58-59 0.5758017147986223 1102 60-61 0.6981640520083658 1102 62-63 0.7502817823387105 1102 64-65 0.70447612021146 1102 66-67 0.4947158712312927 1102 68-69 0.557942010081284 1102 70-71 0.6909680183895546 1102 72-73 0.5562037693711517 1102 74-75 0.46601481288612945 1102 76-77 0.249728993666956 1102 78-79 0.2993595007476344 1102 80-81 0.23444697013498939 1102 82-83 -0.04285721431441303 1102 84-85 -0.14370807522198703 1102 86-87 0.03410118357463787 1102 88-89 -0.18259147529252573 1102 90-91 -0.36937811250574626 1102 92-93 -0.22954743773319564 1102 94-95 -0.3572199008971104 1102 96-97 -0.4896364540150593 1102 98-99 -0.5267567946841218 1102 100-101 -0.08372818364570023 1103 1 0.24503262585513852 1103 2 0.10693023023974035 1103 3 0.27589960750124476 1103 4 0.2815197019338669 1103 5 0.28075866660728366 1103 6 0.25556229264726227 1103 7 0.22345579419902606 1103 8 0.26327278900713935 1103 9 0.30540080615217136 1103 10-11 0.27223640392702464 1103 12-13 0.3272366728286258 1103 14-15 0.33749426691741036 1103 16-17 0.2880640132809944 1103 18-19 0.45928618897330153 1103 20-21 0.5669350772014354 1103 22-23 0.47848637907711833 1103 24-25 0.42203417723955994 1103 26-27 0.38355081909571 1103 28-29 0.317567992387076 1103 30-31 0.24058525365828132 1103 32-33 0.39707875883160426 1103 34-35 0.08496926702941465 1103 36-37 0.07741954312915311 1103 38-39 0.07612083341271614 1103 40-41 0.026887795927564184 1103 42-43 0.04178907615251859 1103 44-45 -0.09300410774274326 1103 46-47 -0.10870609859919966 1103 48-49 -0.10660079023652713 1103 50-51 0.03298027628459721 1103 52-53 -0.029939374558651366 1103 54-55 -0.08297126104677233 1103 56-57 -0.060946084727035554 1103 58-59 -0.15832177996010444 1103 60-61 -0.043583110774278566 1103 62-63 -0.10899007073241762 1103 64-65 -0.3505561504287513 1103 66-67 -0.2651873168480634 1103 68-69 -0.3239766852318908 1103 70-71 -0.22161536547945815 1103 72-73 -0.4433554873212131 1103 74-75 -0.4172867551637722 1103 76-77 -0.5299697414972115 1103 78-79 -0.5191419720064125 1103 80-81 -0.5309660507434621 1103 82-83 -0.3784393853271517 1103 84-85 -0.4055734914915874 1103 86-87 -0.6550881519533895 1103 88-89 -0.6966497790263873 1103 90-91 -0.6729376290958875 1103 92-93 -0.792664694945362 1103 94-95 -0.7636047228671323 1103 96-97 -0.8572456110799429 1103 98-99 -1.021332511568815 1103 100-101 -1.399528885369687 1104 1 0.3490706727047481 1104 2 0.39627365200864517 1104 3 0.39120696578559233 1104 4 0.39391742148604436 1104 5 0.4488994939659037 1104 6 0.38003110950671015 1104 7 0.29023822749255146 1104 8 0.4864055774074103 1104 9 0.5179162616298427 1104 10-11 0.4790074856205706 1104 12-13 0.4950905452434 1104 14-15 0.5787358112413941 1104 16-17 0.6006993146469881 1104 18-19 0.5047885922209545 1104 20-21 0.47659490899520307 1104 22-23 0.6931750962619176 1104 24-25 0.7106855312027065 1104 26-27 0.7212946780489133 1104 28-29 0.557942341991037 1104 30-31 0.5151346710975488 1104 32-33 0.5355397941481641 1104 34-35 0.40304510753311007 1104 36-37 0.3836205128680703 1104 38-39 0.49634102913286426 1104 40-41 0.336082802911541 1104 42-43 0.22942912493486034 1104 44-45 0.2918309564634214 1104 46-47 0.16849132218224128 1104 48-49 0.23627766324511867 1104 50-51 0.3404714882894453 1104 52-53 0.39096655907879807 1104 54-55 0.24539275817920725 1104 56-57 0.14420454551886053 1104 58-59 0.06714462732179527 1104 60-61 0.11382855563216054 1104 62-63 0.028248241128530083 1104 64-65 0.2433076556909839 1104 66-67 0.310671121526255 1104 68-69 0.014690282456136572 1104 70-71 0.09869598063462348 1104 72-73 -0.018132569749020888 1104 74-75 -1.41775065856109E-5 1104 76-77 -0.011763927283659825 1104 78-79 -0.04559035598588679 1104 80-81 0.15475920677750565 1104 82-83 -0.05505593334357073 1104 84-85 -0.014024525584368064 1104 86-87 0.06171158303528301 1104 88-89 -0.10978260962591335 1104 90-91 -0.15217981484857646 1104 92-93 -0.20674067892652204 1104 94-95 -0.0881148949979007 1104 96-97 -0.22542568374706562 1104 98-99 -0.09308285232781088 1104 100-101 -0.2925875296763749 1105 1 -0.03385212392106496 1105 2 0.19425588214998868 1105 3 0.09957340691791927 1105 4 0.029852024553633782 1105 5 0.16072921152628084 1105 6 0.0392695899991935 1105 7 0.04105087346784586 1105 8 0.0352124927236801 1105 9 0.20822791637770166 1105 10-11 0.24241313441303447 1105 12-13 0.18903603174744887 1105 14-15 0.09718989774705733 1105 16-17 0.10711018845208997 1105 18-19 0.05339084114344672 1105 20-21 0.038259689520593554 1105 22-23 0.10647881316415209 1105 24-25 0.07346072724100594 1105 26-27 0.040825069001854786 1105 28-29 0.20606462815479176 1105 30-31 0.08182381093686786 1105 32-33 0.251759806508403 1105 34-35 0.18898613150717125 1105 36-37 0.20140521987408277 1105 38-39 0.17213140258884607 1105 40-41 0.16017203745922615 1105 42-43 0.0951980686154883 1105 44-45 0.10790409058813566 1105 46-47 0.2413184473016372 1105 48-49 0.3975286966097613 1105 50-51 0.3235604258702054 1105 52-53 0.204892702602109 1105 54-55 0.14030258457394496 1105 56-57 -0.07120482739935596 1105 58-59 0.005038409476490813 1105 60-61 0.008353127638613955 1105 62-63 0.22388122211462047 1105 64-65 -4.068651122679512E-5 1105 66-67 -0.13536115958103068 1105 68-69 0.09082016134178872 1105 70-71 -0.10118884435554776 1105 72-73 -0.14152032026470351 1105 74-75 -0.004713478430389273 1105 76-77 -0.21998389148710373 1105 78-79 -0.21475114351006397 1105 80-81 -0.24969168532719266 1105 82-83 -0.21827738238163974 1105 84-85 -0.055577823121147674 1105 86-87 -0.2059198248287224 1105 88-89 -0.08448223159504664 1105 90-91 -0.20974926096512903 1105 92-93 0.09275648383543 1105 94-95 -0.03397060117722006 1105 96-97 -0.27727651003746345 1105 98-99 -0.38211393754126277 1105 100-101 -0.19286683910788227 1106 1 0.3612914756874588 1106 2 0.3742025596161511 1106 3 0.340998491007781 1106 4 0.3576731128283015 1106 5 0.3409740002048096 1106 6 0.26985017564710745 1106 7 0.3692150708737145 1106 8 0.38211832332900997 1106 9 0.3779600662657714 1106 10-11 0.39688817744229965 1106 12-13 0.468222806859103 1106 14-15 0.5728066504722449 1106 16-17 0.6191724430728129 1106 18-19 0.6705682341890196 1106 20-21 0.7469600371295186 1106 22-23 0.6804863278165953 1106 24-25 0.708974772216429 1106 26-27 0.7248581199732556 1106 28-29 0.6740907944445951 1106 30-31 0.804892556507383 1106 32-33 0.5842072433144523 1106 34-35 0.688974882027459 1106 36-37 0.6567560348988835 1106 38-39 0.7961822277381785 1106 40-41 0.6029909320853548 1106 42-43 0.3758725874189892 1106 44-45 0.4264640446902561 1106 46-47 0.6201671755479481 1106 48-49 0.48410469246994836 1106 50-51 0.44408172676003943 1106 52-53 0.41882968301676726 1106 54-55 0.5129894103082648 1106 56-57 0.6466047864060016 1106 58-59 0.7866185113967745 1106 60-61 0.8518503658913446 1106 62-63 0.6784670725190409 1106 64-65 0.5573316600177449 1106 66-67 0.5640274311966422 1106 68-69 0.6618606257203155 1106 70-71 0.6603868702676507 1106 72-73 0.6264169751353847 1106 74-75 0.4848076312182883 1106 76-77 0.528623985398589 1106 78-79 0.5685529411760157 1106 80-81 0.7577864063110695 1106 82-83 0.8823632754879291 1106 84-85 0.7351987284633452 1106 86-87 0.7399786148684413 1106 88-89 0.6434519139507202 1106 90-91 0.807774616055557 1106 92-93 0.7006980540378116 1106 94-95 0.3792790360270608 1106 96-97 0.40680716359262803 1106 98-99 0.5592312752426452 1106 100-101 0.4928733204097391 1107 1 0.013335281600312499 1107 2 0.06296019092160776 1107 3 -0.09993994848926491 1107 4 0.1435979130260563 1107 5 0.23494433320564667 1107 6 0.17330447833683849 1107 7 0.22080521993985514 1107 8 0.1252399211202615 1107 9 0.23334693667763418 1107 10-11 0.2766412599935677 1107 12-13 0.24203239284065603 1107 14-15 0.23933492220677266 1107 16-17 0.23695523440285626 1107 18-19 0.2166580986316191 1107 20-21 0.3137238413711856 1107 22-23 0.2510033329850856 1107 24-25 0.24214081305947133 1107 26-27 0.3112470219182626 1107 28-29 0.19439481068293674 1107 30-31 0.264382009769065 1107 32-33 0.1408760511857281 1107 34-35 0.10964412162129378 1107 36-37 0.07992073531302424 1107 38-39 0.28194060585401814 1107 40-41 0.07648264492718226 1107 42-43 0.04613839272952447 1107 44-45 0.1481387800425935 1107 46-47 0.33798077933457904 1107 48-49 0.3040572378051678 1107 50-51 0.15522365032774132 1107 52-53 0.15853579833195397 1107 54-55 0.2764037090289406 1107 56-57 0.25043319061668967 1107 58-59 0.06451031267210539 1107 60-61 0.09416248389086945 1107 62-63 0.15100655859511392 1107 64-65 0.0440796924596043 1107 66-67 0.10877251469491966 1107 68-69 0.3573291056217158 1107 70-71 0.27851869535734863 1107 72-73 0.17631487623262387 1107 74-75 0.32725936272167644 1107 76-77 0.304311912660296 1107 78-79 0.10412217955127545 1107 80-81 0.3881196160464171 1107 82-83 0.28817812569935697 1107 84-85 0.0705818735273418 1107 86-87 0.29990187966582127 1107 88-89 0.3025286195594603 1107 90-91 0.1760121957632066 1107 92-93 0.4183575955414902 1107 94-95 0.5312601725366868 1107 96-97 0.6780995285444185 1107 98-99 0.7374891702818402 1107 100-101 0.9197207186720533 1108 1 -0.05359412033648425 1108 2 0.05268661076327419 1108 3 0.10862070399766566 1108 4 0.12961913446272888 1108 5 0.2451116234948998 1108 6 0.2069933844978351 1108 7 0.22405204328443062 1108 8 0.27696206611625485 1108 9 0.32435099396122524 1108 10-11 0.20387089759493904 1108 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sequence quality scores pass #Quality Count 2 2068.0 3 401.0 4 393.0 5 430.0 6 545.0 7 628.0 8 749.0 9 913.0 10 1134.0 11 1425.0 12 1809.0 13 3085.0 14 2446.0 15 2067.0 16 2414.0 17 3014.0 18 3033.0 19 2567.0 20 2780.0 21 3114.0 22 3365.0 23 3900.0 24 4536.0 25 5414.0 26 6388.0 27 7789.0 28 9422.0 29 11527.0 30 14456.0 31 17786.0 32 22750.0 33 30479.0 34 43114.0 35 64789.0 36 108731.0 37 187370.0 38 182147.0 39 25505.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.116441527281474 17.186265005384417 12.539168114021738 24.158125353312375 2 15.742662204899267 19.713073642134603 39.665699269730176 24.878564883235956 3 21.40732336992934 23.202369573290486 28.32487452159964 27.065432535180534 4 13.051934874971124 15.032563790698358 36.39116878674833 35.52433254758219 5 14.46972385253414 37.331987102736456 33.273637962681676 14.924651082047735 6 33.308752514962194 34.03839455785784 17.709288870120144 14.943564057059818 7 30.86064543623022 31.37777528264789 19.4293070868352 18.33227219428669 8 26.753417770620825 33.7594328427532 19.38744261182241 20.09970677480356 9 27.26461987557987 16.92430173157301 19.37761866608148 36.43345972676564 10-11 26.161528593509487 24.89571711349308 26.898573689518635 22.044180603478797 12-13 27.888470614353217 22.33432915420296 26.510780191521675 23.266420039922146 14-15 24.118614484425198 24.148725512461628 25.48509376523095 26.247566237882225 16-17 23.489251770109078 27.242520890476495 25.9348727435096 23.33335459590483 18-19 23.257404424312988 27.121772277069812 26.912582337836326 22.708240960780874 20-21 24.821557431805175 25.592750896280318 25.891989528561975 23.69370214335253 22-23 23.902538628177272 25.775387079381183 25.912448115570275 24.409626176871267 24-25 24.264803016794932 25.711488671383652 26.247239082529816 23.776469229291603 26-27 24.24218444374529 25.37985706180906 26.999287159749606 23.378671334696048 28-29 23.38691407957535 26.483638291993262 26.842403175917955 23.287044452513427 30-31 23.546242112510424 26.07311014505515 26.33153330436442 24.049114438070006 32-33 23.54830868095809 25.926497788910673 26.439281655965857 24.085911874165376 34-35 24.33976283897503 25.683896991492695 26.148780687439572 23.827559482092706 36-37 24.289262369940936 25.490935733563212 26.657896793018597 23.56190510347725 38-39 24.103240512488874 25.606003367930384 26.90905947662618 23.38169664295456 40-41 23.82403682116577 25.933956194847468 26.69310467338179 23.54890231060497 42-43 23.409540324687633 26.02521477641225 26.11928351510991 24.44596138379021 44-45 23.82901030469651 25.795362342229144 25.975827414424785 24.39979993864956 46-47 24.102520748994014 26.08343404416931 25.802276289050667 24.011768917786014 48-49 24.126439387825428 25.63682783198634 26.282398635968267 23.954334144219967 50-51 23.535028848869583 25.98799933137485 26.800020238079224 23.676951581676338 52-53 23.813104319751396 26.226380412498568 26.226892785342038 23.733622482408 54-55 22.995672237641415 25.61660047158393 26.659477210405587 24.728250080369072 56-57 23.85739904744039 25.842165008320013 26.02245858721798 24.277977357021623 58-59 24.23894243756057 25.481842028786982 26.16861494500824 24.11060058864421 60-61 23.87361591446423 25.554514014415926 26.988108301793833 23.58376176932601 62-63 23.675136784343355 25.428281463600477 26.775475183526332 24.12110656852984 64-65 23.33151645153377 25.978249939312732 26.624833550355703 24.065400058797795 66-67 23.359803323086542 25.68221023240083 26.271203268895338 24.68678317561729 68-69 23.080431846777934 25.7678144483652 26.6567750896264 24.494978615230465 70-71 23.5475216776336 25.57140807248427 26.393736035094197 24.48733421478794 72-73 23.507362304284662 25.05329247576858 26.80092698162178 24.638418238324977 74-75 23.031776383430543 26.241251965805397 26.265081851172116 24.461889799591948 76-77 23.374346914438906 25.344010697858504 26.449853147811563 24.831789239891023 78-79 23.367000521207512 25.305136903412695 26.820032296937168 24.507830278442626 80-81 23.358273703757064 25.851926555571563 26.540716195232832 24.249083545438538 82-83 23.30136879275476 25.47853952746103 26.715986070000934 24.504105609783274 84-85 23.282573515709203 25.29238820469832 26.601354947080985 24.823683332511493 86-87 23.166315185461038 25.508313549840633 26.751999856547 24.57337140815133 88-89 23.402267076409107 25.27729990149272 26.40949307823066 24.910939943867504 90-91 23.274861967817795 24.957640663425686 26.835800798053107 24.93169657070342 92-93 23.37079443367976 25.441478373660864 26.765224910724296 24.42250228193508 94-95 23.270067587520746 25.372705044625953 26.746235155375707 24.610992212477587 96-97 22.839536224457113 25.20716161680866 26.99064762026776 24.962654538466467 98-99 23.217220753965254 25.457184473855133 26.248441889691556 25.077152882488058 100-101 23.45222632963666 24.64884168351048 26.697510701906747 25.201421284946115 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 651.0 1 350.5 2 52.0 3 52.5 4 46.5 5 46.5 6 64.5 7 96.0 8 142.5 9 176.5 10 193.5 11 237.0 12 277.5 13 306.5 14 345.5 15 399.0 16 449.0 17 473.0 18 483.0 19 471.5 20 452.0 21 468.0 22 477.0 23 521.5 24 599.0 25 741.5 26 1056.5 27 1458.0 28 1901.0 29 2483.0 30 3286.5 31 4252.0 32 5432.5 33 6807.0 34 8675.5 35 10650.0 36 12670.5 37 14998.0 38 16885.0 39 18113.5 40 19583.0 41 21731.5 42 23542.5 43 25461.0 44 27343.0 45 28937.0 46 31147.0 47 36364.5 48 49965.5 49 50488.5 50 38499.5 51 34055.5 52 31822.0 53 30632.0 54 29781.0 55 27738.5 56 24890.0 57 22715.5 58 20902.5 59 18429.0 60 15699.0 61 13290.5 62 10795.5 63 8429.0 64 6292.0 65 4714.0 66 3530.0 67 2830.0 68 2187.5 69 1440.5 70 1021.5 71 802.5 72 664.0 73 551.5 74 403.0 75 295.0 76 207.0 77 132.0 78 78.0 79 54.0 80 35.5 81 21.5 82 16.5 83 9.5 84 9.5 85 6.5 86 3.0 87 4.0 88 2.5 89 0.5 90 1.0 91 1.0 92 0.0 93 1.5 94 3.0 95 2.0 96 1.5 97 1.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3313001523292351 2 0.11969636130709956 3 0.11268539232745049 4 0.120588666449964 5 0.13550290955212657 6 0.08617118236805037 7 0.09381951216403119 8 0.10032059249061487 9 0.08782832049051288 10-11 0.0866173349394826 12-13 0.09044149983747299 14-15 0.09165248538850328 16-17 0.08094482367413017 18-19 0.09528544204159417 20-21 0.1057381594294346 22-23 0.11376890571521445 24-25 0.12804578800104527 26-27 0.12740842718471354 28-29 0.12281942930712506 30-31 0.11300407273561637 32-33 0.10471838212330384 34-35 0.3274759874312447 36-37 0.48209972147332325 38-39 0.4576250661261847 40-41 0.4349987571464081 42-43 0.4682052556772915 44-45 0.47477007208550837 46-47 0.4824184018814891 48-49 0.4917876058815656 50-51 0.4816535689018911 52-53 0.4848403729835497 54-55 0.473176670044679 56-57 0.4508053053914352 58-59 0.4293262458810557 60-61 0.3897461391868551 62-63 0.45921846816701406 64-65 0.48993925951420364 66-67 0.4991172552693805 68-69 0.5128205128205128 70-71 0.504407350044934 72-73 0.49548429861628973 74-75 0.5038337253102354 76-77 0.48031511118759435 78-79 0.4596646207384462 80-81 0.46176791143234097 82-83 0.47470633600387513 84-85 0.4737502947793776 86-87 0.4769370988610362 88-89 0.4884733296366406 90-91 0.5048535026163661 92-93 0.4968227563305863 94-95 0.5024315315143055 96-97 0.5016029624530743 98-99 0.49612165943262143 100-101 0.48222719363658956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 784485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.73793825715193 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0999040316939 41.8591381403659 2 13.639394419830678 15.204634480749297 3 4.861454515200244 8.129023548245511 4 2.2489244608671823 5.014016509792548 5 1.2052764990445337 3.358981354327023 6 0.7471192705272042 2.4985732662824214 7 0.46310610320660983 1.8068805570928177 8 0.3102801847292544 1.38355022230855 9 0.229295028503266 1.1502388927248242 >10 1.1130614503472656 10.788756462954831 >50 0.0547134767289344 2.0565042705416188 >100 0.02106076215254437 2.2805238127097494 >500 0.0034338199116495756 1.3267435006808448 >1k 0.0029759772567629654 3.1424349812240666 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4960 0.6322619298010798 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2687 0.34251770269667364 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2451 0.31243427216581576 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2254 0.2873222560023455 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2188 0.2789090932267666 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1869 0.23824547314480202 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1452 0.18508958106273543 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1328 0.16928303281770843 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1169 0.1490149588583593 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1132 0.14429848881750448 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1081 0.13779740849092081 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1050 0.13384577142966403 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1007 0.12836446840921112 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 962 0.12262822106222553 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 959 0.1222458045724265 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 942 0.12007877779689859 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 845 0.10771397796006296 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.274721632663467E-4 0.0 2 0.0 0.0 0.0 1.274721632663467E-4 0.0 3 0.0 0.0 0.0 1.274721632663467E-4 0.0 4 0.0 0.0 0.0 2.549443265326934E-4 0.0 5 0.0 0.0 0.0 2.549443265326934E-4 0.0 6 0.0 0.0 0.0 2.549443265326934E-4 0.0 7 0.0 0.0 0.0 3.8241648979904015E-4 0.0 8 0.0 0.0 0.0 3.8241648979904015E-4 0.0 9 0.0 0.0 0.0 8.92305142864427E-4 0.0 10-11 0.0 0.0 0.0 8.92305142864427E-4 0.0 12-13 0.0 0.0 0.0 0.0010197773061307736 0.0 14-15 0.0 0.0 0.0 0.0010197773061307736 0.0 16-17 0.0 0.0 0.0 0.0011472494693971205 0.0 18-19 0.0 0.0 0.0 0.0014021937959298137 0.0 20-21 0.0 0.0 0.0 0.0016571381224625072 0.0 22-23 0.0 0.0 0.0 0.0019120824489952007 0.0 24-25 0.0 0.0 0.0 0.0024219711020605876 0.0 26-27 0.0 0.0 0.0 0.0025494432653269342 0.0 28-29 0.0 0.0 0.0 0.0038241648979904013 0.0 30-31 0.0 0.0 0.0 0.010835133877639472 0.0 32-33 0.0 0.0 0.0 0.027980139836963104 0.0 34-35 0.0 0.0 0.0 0.05557786318412717 0.0 36-37 0.0 0.0 0.0 0.1021689388579769 0.0 38-39 0.0 0.0 0.0 0.17278851730753297 0.0 40-41 0.0 0.0 0.0 0.2617003511858098 0.0 42-43 0.0 0.0 0.0 0.35124954588041835 0.0 44-45 0.0 0.0 0.0 0.47043601853445255 0.0 46-47 0.0 0.0 0.0 0.6252509608214306 0.0 48-49 0.0 0.0 0.0 0.7971471729860992 0.0 50-51 0.0 0.0 0.0 0.9835752117631312 0.0 52-53 0.0 0.0 0.0 1.1668164464585047 0.0 54-55 0.0 0.0 0.0 1.3762532107051122 0.0 56-57 0.0 0.0 0.0 1.6420326711154452 0.0 58-59 0.0 0.0 0.0 1.9315856899749515 0.0 60-61 0.0 0.0 0.0 2.263395730957252 0.0 62-63 0.0 0.0 0.0 2.6046387120212624 0.0 64-65 0.0 0.0 0.0 2.962325602146631 0.0 66-67 0.0 0.0 0.0 3.384067254313339 0.0 68-69 0.0 0.0 0.0 3.867505433500959 0.0 70-71 0.0 0.0 0.0 4.387145707056222 0.0 72-73 0.0 0.0 0.0 4.913860685672766 0.0 74-75 0.0 0.0 0.0 5.486848059554994 0.0 76-77 0.0 0.0 0.0 6.104514426662078 0.0 78-79 0.0 0.0 0.0 6.841940891157893 0.0 80-81 0.0 0.0 0.0 7.613784839735622 0.0 82-83 0.0 0.0 0.0 8.388560648068477 0.0 84-85 0.0 0.0 0.0 9.209800059911917 0.0 86-87 0.0 0.0 0.0 10.093309623510965 0.0 88-89 0.0 0.0 0.0 11.054959623192286 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATAGCG 65 0.005923293 29.149189 5 TGTACGC 65 0.005923293 29.149189 2 GTCGGGA 200 2.3646862E-11 28.420458 2 TACATGG 4105 0.0 26.654999 2 GTACATG 4190 0.0 26.399323 1 ACATGGG 4215 0.0 24.94797 3 GGTCGGG 280 1.8189894E-12 23.736729 1 CATGGGA 2625 0.0 23.638603 4 ATGGGAG 945 0.0 23.558403 5 AAGACCG 125 2.2980885E-4 22.736366 5 GAGTACG 105 0.0022027406 22.60641 1 TACCTGG 650 0.0 21.86189 2 CGGGAGT 325 1.8189894E-12 21.86189 4 GTAAGTA 240 6.1136234E-9 21.710072 2 CATGGGG 1570 0.0 21.42094 4 ATGGGAT 895 0.0 21.169802 5 CTTAGAC 225 7.35854E-8 21.05219 3 GTATAGC 250 9.53878E-9 20.888323 1 CAGTGCG 115 0.0037913925 20.586653 9 GTATTAG 165 6.3466374E-5 20.140255 1 >>END_MODULE