FastQCFastQC Report
Fri 27 May 2016
SRR939115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784485
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55410.7063232566588271No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25050.31931776898219855No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24720.31511118759440904No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20060.2557091595122915No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19590.24971796783877323No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19530.2489531348591751No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14900.1899335232668566No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12360.15755559379720452No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11760.14990726400122373No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11150.14213146204197658No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11140.14200398987871024No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10950.13958201877664964No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10640.1356303817153929No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10370.13218863330720154No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9970.12708974677654766No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9600.12237327673569284No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8760.11166561502131973No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8470.10796892228659566No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG702.0660655E-433.8912167
TATAGGG1351.2090823E-831.6318022
ACAGCGT1755.014954E-927.1129728
GTACATG45250.027.03091
TACATGG45700.026.6828422
ACATGGG44650.025.9288653
GTATAGG1701.10076144E-725.1964591
ATGGGAG10500.023.0460265
CATGGGG15500.022.9585654
CATGGGA29450.022.0724454
GTGTAGC5600.021.2470151
CCGTCTG1150.003746071820.6294359
GTATATA2551.1637894E-820.5304471
CGGGAGT2851.9881554E-919.977984
GTATAGC3102.5283953E-1019.9584871
ACCTAAT1200.004708403719.8305471
GTATCGT1456.1893975E-419.6335326
GTATAGA2702.2448148E-819.3898681
ATGCACT5700.019.1455634
GTCGGGA2502.1951382E-718.979082
Produced by FastQC (version 0.11.5)