##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939115_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 784485 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49993180239265 34.0 31.0 34.0 31.0 34.0 2 32.690868531584414 34.0 31.0 34.0 31.0 34.0 3 32.793860940617094 34.0 31.0 34.0 31.0 34.0 4 36.234757834757836 37.0 35.0 37.0 35.0 37.0 5 36.196433328871805 37.0 35.0 37.0 35.0 37.0 6 36.23896696558889 37.0 37.0 37.0 35.0 37.0 7 36.19738427120978 37.0 37.0 37.0 35.0 37.0 8 36.18883216377623 37.0 37.0 37.0 35.0 37.0 9 37.934113462972526 39.0 38.0 39.0 35.0 39.0 10-11 37.86459141984869 39.0 38.0 39.0 35.0 39.0 12-13 37.80873248054456 39.0 38.0 39.0 35.0 39.0 14-15 39.16781455349688 40.5 39.0 41.0 36.0 41.0 16-17 39.12076649011772 40.0 38.0 41.0 36.0 41.0 18-19 39.142144209258305 40.5 38.5 41.0 36.0 41.0 20-21 39.19815738987998 41.0 39.0 41.0 36.0 41.0 22-23 39.16488970471073 41.0 39.0 41.0 36.0 41.0 24-25 39.10648769574944 41.0 39.0 41.0 35.5 41.0 26-27 38.97676182463654 40.0 38.5 41.0 35.0 41.0 28-29 38.84599131914568 40.0 38.0 41.0 35.0 41.0 30-31 38.727757700912065 40.0 38.0 41.0 35.0 41.0 32-33 38.664868034442975 40.0 38.0 41.0 34.5 41.0 34-35 38.660050224032325 40.0 38.0 41.0 35.0 41.0 36-37 38.58360771716477 40.0 38.0 41.0 34.5 41.0 38-39 38.47703397770512 40.0 38.0 41.0 34.0 41.0 40-41 38.371667399631605 40.0 38.0 41.0 34.0 41.0 42-43 38.26626512935238 40.0 38.0 41.0 34.0 41.0 44-45 38.134418121442735 40.0 38.0 41.0 33.0 41.0 46-47 37.95242165242165 40.0 38.0 41.0 33.0 41.0 48-49 37.73773558449174 40.0 37.0 41.0 33.0 41.0 50-51 37.53652077477581 40.0 37.0 41.0 33.0 41.0 52-53 37.32691702199532 40.0 36.5 41.0 32.0 41.0 54-55 37.10675347520985 39.0 36.0 41.0 32.0 41.0 56-57 36.8490633982804 39.0 35.5 41.0 31.0 41.0 58-59 36.57298482443896 39.0 35.0 40.0 31.0 41.0 60-61 36.18815464922848 38.0 35.0 40.0 30.5 41.0 62-63 35.819555504566694 38.0 35.0 40.0 30.0 41.0 64-65 35.397993588150186 37.0 34.0 40.0 29.0 41.0 66-67 35.029082774049215 37.0 34.0 39.0 29.0 41.0 68-69 34.56135171481928 36.0 34.0 39.0 28.5 40.0 70-71 34.12099084112507 35.5 34.0 38.5 28.0 40.0 72-73 33.66831870590259 35.0 33.0 37.5 27.5 39.5 74-75 33.20158702843267 35.0 33.0 37.0 26.0 39.0 76-77 31.177830041364714 33.5 30.0 35.0 24.5 37.0 78-79 32.185906040268456 34.5 32.0 36.0 26.0 37.0 80-81 32.24806975276774 35.0 32.0 35.5 26.0 37.0 82-83 32.00046272395266 35.0 32.0 35.0 26.0 37.0 84-85 31.676379408146744 34.5 32.0 35.0 25.0 36.0 86-87 31.1831252350268 34.0 31.5 35.0 24.0 36.0 88-89 30.69255498830443 34.0 31.0 35.0 21.5 35.5 90-91 30.280073551438207 34.0 31.0 35.0 19.5 35.0 92-93 30.064891616793183 34.0 30.0 35.0 18.5 35.0 94-95 29.790960311541966 34.0 30.0 35.0 16.5 35.0 96-97 29.278276831296964 34.0 30.0 35.0 4.0 35.0 98-99 28.579225224191667 34.0 29.5 35.0 2.0 35.0 100-101 24.96189857039969 30.0 23.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17405885440991398 1101 2 0.27269742752999093 1101 3 0.23780443223052572 1101 4 0.20583160151976188 1101 5 0.27610989881856796 1101 6 0.2619293259528419 1101 7 0.18859267246868683 1101 8 0.20693990422496 1101 9 0.237049962294833 1101 10-11 0.3734382466381305 1101 12-13 0.2940657114222347 1101 14-15 0.4419738207238595 1101 16-17 0.3760975611141433 1101 18-19 0.34195526905993745 1101 20-21 0.35971544524296917 1101 22-23 0.3770923758277078 1101 24-25 0.19624362286043606 1101 26-27 0.2990993097376773 1101 28-29 0.3196105753875642 1101 30-31 0.3792461193258774 1101 32-33 0.383152054892804 1101 34-35 0.4093364961143422 1101 36-37 0.4854289223256174 1101 38-39 0.5345428694905934 1101 40-41 0.5084994111094616 1101 42-43 0.6046306820281941 1101 44-45 0.6023297192471802 1101 46-47 0.5368690689101854 1101 48-49 0.532087422411351 1101 50-51 0.5978780447071372 1101 52-53 0.6060604233276834 1101 54-55 0.7430229864863307 1101 56-57 0.47498122694720024 1101 58-59 0.5366623017602947 1101 60-61 0.6011640608044999 1101 62-63 0.5015473473448466 1101 64-65 0.5389144055387405 1101 66-67 0.4937088832034249 1101 68-69 0.40017364683477297 1101 70-71 0.45637580650707577 1101 72-73 0.4675366922044617 1101 74-75 0.36406806206083786 1101 76-77 0.5122412846905782 1101 78-79 0.522245958144623 1101 80-81 0.4411733856177662 1101 82-83 0.4465521415305389 1101 84-85 0.39721181436027564 1101 86-87 0.7530099733809941 1101 88-89 0.6960156239631203 1101 90-91 0.6277339241288473 1101 92-93 0.7547133387491023 1101 94-95 0.5759211064873746 1101 96-97 0.634783448901036 1101 98-99 0.21462142127071715 1101 100-101 -0.26904701698533273 1102 1 0.29485070961352733 1102 2 0.2586702782087258 1102 3 0.20454651367849408 1102 4 0.11789042504917546 1102 5 0.19002392596789264 1102 6 0.20971213138270173 1102 7 0.20402253672207848 1102 8 0.14001682730188492 1102 9 0.2769594645572724 1102 10-11 0.17984096157025675 1102 12-13 0.20994806436340951 1102 14-15 0.29424757637996635 1102 16-17 0.3089256154127824 1102 18-19 0.2370910156662731 1102 20-21 0.32241906515247365 1102 22-23 0.38994305456073874 1102 24-25 0.3828838943536397 1102 26-27 0.3148685405069145 1102 28-29 0.34136396905272903 1102 30-31 0.261531187199175 1102 32-33 0.3303126883769636 1102 34-35 0.3673455458880994 1102 36-37 0.3425895558097878 1102 38-39 0.43212205501095724 1102 40-41 0.41543380024972976 1102 42-43 0.24826190374764678 1102 44-45 0.41640211743722944 1102 46-47 0.34682382004140777 1102 48-49 0.2629697753525235 1102 50-51 0.29863596325917996 1102 52-53 0.3191486586218062 1102 54-55 0.2432039819614431 1102 56-57 0.1815988740060206 1102 58-59 0.08710347823087972 1102 60-61 0.15860749971852783 1102 62-63 0.3190699717792356 1102 64-65 0.18074698472425865 1102 66-67 0.28839214112198164 1102 68-69 0.2053546423098851 1102 70-71 0.25579888343015256 1102 72-73 0.08750275555288312 1102 74-75 0.21022190821468456 1102 76-77 0.18711685030143954 1102 78-79 0.24553781334824976 1102 80-81 0.1246801729480822 1102 82-83 0.4191313270509056 1102 84-85 0.3669855700163822 1102 86-87 0.5673878014352951 1102 88-89 0.35444322577307474 1102 90-91 0.417496367567761 1102 92-93 0.3511160536812312 1102 94-95 0.38976726033352804 1102 96-97 0.32553005523587686 1102 98-99 0.26648793710782215 1102 100-101 0.2613715350508663 1103 1 0.019174975402322048 1103 2 0.11576981905599126 1103 3 0.12504811638142854 1103 4 0.18618506249178068 1103 5 0.20533840227388112 1103 6 0.12350403989869108 1103 7 0.17373145121571554 1103 8 0.13169576691896623 1103 9 0.2183958329190503 1103 10-11 0.2668482387079152 1103 12-13 0.2748315435282791 1103 14-15 0.38997767254213755 1103 16-17 0.3898859376860244 1103 18-19 0.28557711113538886 1103 20-21 0.4071879727154908 1103 22-23 0.3215899401190754 1103 24-25 0.23711481352543728 1103 26-27 0.31534718557318797 1103 28-29 0.38956525987833146 1103 30-31 0.476603092249853 1103 32-33 0.4805996588352457 1103 34-35 0.4948513935880072 1103 36-37 0.4140105468866224 1103 38-39 0.43281294992562636 1103 40-41 0.40266957084663346 1103 42-43 0.4574980208749153 1103 44-45 0.34366539388182815 1103 46-47 0.2869823576832715 1103 48-49 0.37649095702106905 1103 50-51 0.4016063782292889 1103 52-53 0.348947021265829 1103 54-55 0.408048476460273 1103 56-57 0.39309610176309917 1103 58-59 0.5094016242934387 1103 60-61 0.5244720929785132 1103 62-63 0.551328700012796 1103 64-65 0.525607506252463 1103 66-67 0.3567257632306138 1103 68-69 0.7316497995480447 1103 70-71 0.6062591190707991 1103 72-73 0.5431003038505509 1103 74-75 0.11596564900997208 1103 76-77 0.23492396283717554 1103 78-79 0.42451966382039075 1103 80-81 0.12240823324437144 1103 82-83 0.4242773029670417 1103 84-85 0.22875027589873298 1103 86-87 0.49648227427598 1103 88-89 0.594724131949981 1103 90-91 0.5814214736926857 1103 92-93 0.4579318727923827 1103 94-95 0.18623265061335204 1103 96-97 0.44148561271660114 1103 98-99 0.39308522550771485 1103 100-101 -0.06583867893163386 1104 1 0.21363125908260372 1104 2 0.24223677070830263 1104 3 0.23410154486001744 1104 4 0.0896711430670365 1104 5 0.27286987236937676 1104 6 0.1977283120670137 1104 7 0.23728564139088348 1104 8 0.2900168273018835 1104 9 0.2723243726981792 1104 10-11 0.28241554441198957 1104 12-13 0.22784794637925643 1104 14-15 0.36299652294999873 1104 16-17 0.31680948529311337 1104 18-19 0.19898634686465755 1104 20-21 0.2421245261334235 1104 22-23 0.3368080469760386 1104 24-25 0.40092072226532594 1104 26-27 0.3308427525412938 1104 28-29 0.2688479569172131 1104 30-31 0.3895987752659238 1104 32-33 0.1729955638193985 1104 34-35 0.2407316911771602 1104 36-37 0.2481664140602433 1104 38-39 0.36056887786617153 1104 40-41 0.2779043042106366 1104 42-43 0.3015802081467669 1104 44-45 0.18214457487866298 1104 46-47 0.3036753285531191 1104 48-49 0.1169011759930143 1104 50-51 0.12169554525817006 1104 52-53 0.10826672705261586 1104 54-55 0.14831943788593804 1104 56-57 0.17383509514203865 1104 58-59 0.1747425309866557 1104 60-61 0.2007320572779463 1104 62-63 0.06310123758068897 1104 64-65 0.028332523237658336 1104 66-67 0.05820083823979871 1104 68-69 -0.007223311507736696 1104 70-71 0.16364272297170146 1104 72-73 -0.0032038852696345543 1104 74-75 0.11136804001984046 1104 76-77 0.09786394283472788 1104 78-79 0.27703326253078586 1104 80-81 0.22248482489482768 1104 82-83 0.30323717569407904 1104 84-85 0.16069869996750086 1104 86-87 0.3920401695458615 1104 88-89 0.3004128027156021 1104 90-91 0.6066186497184454 1104 92-93 0.6741777425401558 1104 94-95 0.6563861715083128 1104 96-97 0.7478669842768326 1104 98-99 0.5354391422317057 1104 100-101 0.5051584050997455 1105 1 -0.025527441646971738 1105 2 -0.056455772211442934 1105 3 0.029896653734510892 1105 4 0.04555148947494558 1105 5 0.06320319767657878 1105 6 0.025118293847690154 1105 7 0.025170996105835286 1105 8 0.05752383010300122 1105 9 -0.008754821157012316 1105 10-11 0.1369137907019109 1105 12-13 0.0637295769684556 1105 14-15 0.12054309458724788 1105 16-17 0.05885558740158103 1105 18-19 0.1560686067026893 1105 20-21 0.07595547971829575 1105 22-23 0.0012875923758599583 1105 24-25 -0.008957842341033029 1105 26-27 0.08930831641728076 1105 28-29 0.07977433319837957 1105 30-31 0.05964043089665694 1105 32-33 0.018653024511415595 1105 34-35 0.13191137221862448 1105 36-37 0.04381504600586794 1105 38-39 -0.053802314736941526 1105 40-41 0.08724752573992589 1105 42-43 -0.030344538829382373 1105 44-45 0.08194833592462913 1105 46-47 -0.20919858892217746 1105 48-49 -0.08261845994159245 1105 50-51 -0.1672954092898422 1105 52-53 -0.06800120132216847 1105 54-55 -0.125283412996545 1105 56-57 -0.15681149013963847 1105 58-59 -0.19745534529852904 1105 60-61 -0.10728885882488726 1105 62-63 -0.051973445587712774 1105 64-65 -0.29117178278274025 1105 66-67 -0.491789931707153 1105 68-69 -0.5370123044688242 1105 70-71 -0.45950223701802884 1105 72-73 -0.47821853296252215 1105 74-75 -0.3577052626536599 1105 76-77 -0.46508903205150176 1105 78-79 -0.1430826348310248 1105 80-81 -0.2647455973600401 1105 82-83 -0.42932805670260166 1105 84-85 -0.3412777353057521 1105 86-87 -0.3283755038868357 1105 88-89 -0.3116982308095544 1105 90-91 -0.3239252010596907 1105 92-93 -0.33788954855966224 1105 94-95 -0.09300584891017039 1105 96-97 -0.44260719966608164 1105 98-99 -0.3030148640126278 1105 100-101 -0.04748700836650244 1106 1 0.26983889765983093 1106 2 0.32556530931353933 1106 3 0.1866356545557224 1106 4 0.19830817447826377 1106 5 0.18774618756329176 1106 6 0.1787411887887984 1106 7 0.2736776276741253 1106 8 0.1876071887476698 1106 9 0.2330977431618848 1106 10-11 0.26923579580917334 1106 12-13 0.3086841853178157 1106 14-15 0.25669835990623113 1106 16-17 0.18492569415914062 1106 18-19 0.323733664693755 1106 20-21 0.35000686734161945 1106 22-23 0.2561923655301044 1106 24-25 0.2891393081657583 1106 26-27 0.33591941065416364 1106 28-29 0.36369151452874604 1106 30-31 0.2678683003576978 1106 32-33 0.3744825048979479 1106 34-35 0.18645728697006803 1106 36-37 0.3470672705904789 1106 38-39 0.6069133771623072 1106 40-41 0.6452639049823858 1106 42-43 0.44400664742825313 1106 44-45 0.4438609724651883 1106 46-47 0.4015328522486712 1106 48-49 0.47191339296021084 1106 50-51 0.4399571302801988 1106 52-53 0.5525932211857878 1106 54-55 0.5319983752207591 1106 56-57 0.6158112135603133 1106 58-59 0.5052968399129085 1106 60-61 0.4239650081837567 1106 62-63 0.5082984543369164 1106 64-65 0.7895291722980886 1106 66-67 0.6703178833064101 1106 68-69 0.6059629579252928 1106 70-71 0.6119066478210513 1106 72-73 0.281613512305384 1106 74-75 0.4270893780942018 1106 76-77 0.6658464746813131 1106 78-79 0.826633765785445 1106 80-81 0.660633791343237 1106 82-83 0.6633001592424108 1106 84-85 0.5386565677327297 1106 86-87 0.5348645712596891 1106 88-89 0.7318203814546251 1106 90-91 0.8379182511806427 1106 92-93 0.6980075411999316 1106 94-95 0.7624580233857365 1106 96-97 0.7463249997196932 1106 98-99 1.0057242943012987 1106 100-101 0.5207748345232659 1107 1 -0.028477906618398663 1107 2 -0.02401158517381674 1107 3 -0.033206871160487594 1107 4 0.030617037150449278 1107 5 0.03206668585468009 1107 6 0.08700884980999746 1107 7 0.09286595445328061 1107 8 -0.011023484791742533 1107 9 0.0858786108894094 1107 10-11 0.08954607898782996 1107 12-13 0.041581966298693374 1107 14-15 0.12901293245737833 1107 16-17 0.19967563453617032 1107 18-19 0.11997367458159403 1107 20-21 0.15769884026147452 1107 22-23 0.018872909987948105 1107 24-25 -0.0742405223831355 1107 26-27 -0.030287506307132617 1107 28-29 0.03401389638386121 1107 30-31 0.0690600635091343 1107 32-33 -0.08385104603748061 1107 34-35 0.10296858574123036 1107 36-37 0.0343425964278552 1107 38-39 -0.016189350175302764 1107 40-41 -0.059396957801212125 1107 42-43 -0.009667607458659688 1107 44-45 -0.23697507219024772 1107 46-47 -0.1801210281189256 1107 48-49 -0.12675523038448944 1107 50-51 -0.29968022277310524 1107 52-53 -0.33656636470872314 1107 54-55 -0.3838670805337827 1107 56-57 -0.4750837925586566 1107 58-59 -0.35619855649718346 1107 60-61 -0.4071444317432622 1107 62-63 -0.4743334168844271 1107 64-65 -0.3807174840281249 1107 66-67 -0.22587199105885247 1107 68-69 -0.278445939040985 1107 70-71 -0.1579364871810327 1107 72-73 -0.3594614072253677 1107 74-75 -0.2780745807320031 1107 76-77 -0.27781373181437274 1107 78-79 -0.19601251939863573 1107 80-81 -0.23579370450926973 1107 82-83 -0.3441670742341927 1107 84-85 -0.11975427546667561 1107 86-87 -0.18242765790839144 1107 88-89 -0.014757608006469525 1107 90-91 -0.01638931284224654 1107 92-93 -0.033782209915496964 1107 94-95 -0.03637058101887547 1107 96-97 -0.4323506913410391 1107 98-99 -0.3114063870717878 1107 100-101 -0.6175095557469632 1108 1 -0.07432703811391406 1108 2 -0.07334835299854348 1108 3 0.021761598946312688 1108 4 -0.04216328992340834 1108 5 -0.032260275068843214 1108 6 0.0119283435380666 1108 7 0.002831792225045149 1108 8 0.06587889626739951 1108 9 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-0.5601718797898911 2316 86-87 -1.0267812134760597 2316 88-89 -1.3107631262287995 2316 90-91 -1.4804650625132503 2316 92-93 -1.164849074885165 2316 94-95 -0.8413704642173734 2316 96-97 -1.2780539532178032 2316 98-99 -1.440575876441553 2316 100-101 -0.8418352865999523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 7.0 5 14.0 6 31.0 7 70.0 8 124.0 9 247.0 10 367.0 11 588.0 12 861.0 13 1090.0 14 1333.0 15 1621.0 16 2110.0 17 3036.0 18 3027.0 19 2399.0 20 2557.0 21 2818.0 22 3144.0 23 3549.0 24 4097.0 25 5243.0 26 6415.0 27 7811.0 28 9788.0 29 12202.0 30 14986.0 31 19118.0 32 24058.0 33 31637.0 34 44449.0 35 67063.0 36 113852.0 37 204037.0 38 177799.0 39 12936.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.69070044663641 17.52983971226997 12.840157371547264 23.939302469546355 2 16.05116732633511 20.195032409797513 39.13369917844191 24.620101085425468 3 21.442602471685245 23.352390421741653 28.325589399414902 26.879417707158197 4 13.267175280598098 15.539494062983996 36.22045035915282 34.972880297265085 5 14.351071084851846 37.57101792895977 33.3198212840271 14.75808970216129 6 33.28425655047579 34.5951802775069 17.277831953447166 14.842731218570146 7 30.949603880252653 31.616283294135645 19.199092398197543 18.235020427414163 8 26.818231068790354 34.1557837307278 18.99360727101219 20.032377929469654 9 26.98101302128148 17.152144400466547 19.26550539525931 36.601337182992665 10-11 26.1754526855198 25.25758937392047 26.659591961605383 21.907365978954346 12-13 27.860953364309072 22.53185210679618 26.404711371154328 23.202483157740428 14-15 24.0965636978522 24.359350242263318 25.3846830436074 26.15940301627709 16-17 23.341098636344803 27.583631038691603 25.73716733218819 23.3381029927754 18-19 23.178072238474922 27.406961254835977 26.828874994423092 22.58609151226601 20-21 24.805190666488205 25.761741779638868 25.87818760078268 23.554879953090243 22-23 23.839971446235428 25.931980853681075 25.82496797261898 24.403079727464515 24-25 24.232583752611593 25.89826675657726 26.25882268959302 23.610326801218125 26-27 24.24156282864336 25.509098441632936 26.994869180024857 23.254469549698843 28-29 23.337603650802755 26.70427095483024 26.717783004136468 23.240342390230534 30-31 23.61285330396251 26.179549545415142 26.33481717173262 23.87277997888972 32-33 23.568838154967906 26.221278928214055 26.307705054908638 23.902177861909404 34-35 24.392658356302 25.808832715877827 26.15062174752232 23.647887180297857 36-37 24.354068510879753 25.57261349943577 26.682520353711485 23.390797635972994 38-39 24.002060426862815 25.904958985902777 26.887231792284496 23.205748794949915 40-41 23.746240666206855 26.111927749397296 26.634250447167894 23.507581137227955 42-43 23.456501313737963 26.204833484828587 26.075945655485768 24.262719545947682 44-45 23.835431686201225 25.88861573723221 25.94929407258072 24.32665850398584 46-47 24.15481907053999 26.233528835134745 25.752189038047003 23.85946305627826 48-49 24.11744085253926 25.86267336324699 26.116790740363044 23.9030950438507 50-51 23.54257888933504 26.19393614919342 26.74767522642243 23.51580973504911 52-53 23.905267666389626 26.41099117381616 26.023156622697215 23.660584537096994 54-55 23.11796911349484 25.77283185784304 26.45767605499149 24.651522973670627 56-57 23.856734035704953 25.932873158823945 25.97806204070186 24.232330764769245 58-59 24.325292465686193 25.54761227916084 26.01303331884337 24.114061936309596 60-61 23.805335005940815 25.789962825278813 26.84171676839996 23.562985400380416 62-63 23.659176304492625 25.692505282346605 26.518176690111893 24.13014172304888 64-65 23.24580720371257 26.278234809339544 26.39104975904129 24.084908227906595 66-67 23.35363615040232 25.78997419868036 26.09999796036999 24.756391690547336 68-69 23.013748922031226 25.93090929255292 26.450182704951104 24.60515908046475 70-71 23.554257604834767 25.820076751038425 26.1862407733996 24.439424870727212 72-73 23.42020561259935 25.20436974575677 26.594453686176283 24.7809709554676 74-75 22.925677355207558 26.351937895562056 26.03892999866154 24.683454750568846 76-77 23.261561378563204 25.49501313945452 26.229149020705023 25.01427646127726 78-79 23.324607813706013 25.485145902288203 26.42207167673384 24.768174607271945 80-81 23.196030377681392 25.936523011125523 26.306332720383196 24.561113890809892 82-83 23.14257652038515 25.698663000546222 26.386825421409455 24.771935057659178 84-85 22.993747490391787 25.559953345188248 26.239443711479502 25.206855452940463 86-87 22.87034916032687 25.682148675007994 26.260626320413827 25.186875844251304 88-89 22.99244778050758 25.44965496321165 26.02728680067094 25.530610455609832 90-91 22.99225305203643 25.061603817229106 26.41148086554403 25.534662265190438 92-93 22.844477587206892 25.54720612886161 26.382977367317412 25.22533891661408 94-95 22.641032014633804 25.550265460780004 26.26130518747968 25.54739733710651 96-97 22.152445331934537 25.455187451122363 26.600780512553147 25.791586704389953 98-99 22.512020824197677 25.642597823759168 25.75649608908922 26.088885262953937 100-101 22.521004332800924 24.709011435586074 26.390186632945863 26.379797598667142 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 37.0 1 25.5 2 15.0 3 16.5 4 16.5 5 22.0 6 34.0 7 71.5 8 112.5 9 133.5 10 127.5 11 113.5 12 131.0 13 149.0 14 161.0 15 161.0 16 172.0 17 191.5 18 205.0 19 228.5 20 241.5 21 269.5 22 326.0 23 419.0 24 548.0 25 695.5 26 1038.0 27 1467.0 28 1880.0 29 2464.5 30 3209.0 31 4173.0 32 5480.5 33 6935.5 34 8879.5 35 10831.0 36 12725.0 37 15067.0 38 16879.0 39 18190.0 40 19712.0 41 21707.5 42 23746.0 43 25732.5 44 27381.0 45 29068.5 46 31195.5 47 36748.5 48 50016.5 49 49945.0 50 38819.0 51 34931.0 52 32409.5 53 30963.5 54 29924.0 55 27952.5 56 25318.5 57 23095.0 58 21153.0 59 18570.0 60 15667.0 61 13222.0 62 10766.0 63 8243.5 64 6165.5 65 4608.0 66 3394.0 67 2700.5 68 2079.5 69 1380.0 70 968.0 71 745.0 72 651.5 73 573.5 74 395.5 75 288.0 76 204.0 77 96.0 78 47.5 79 30.5 80 18.0 81 9.5 82 6.0 83 3.5 84 2.5 85 1.0 86 0.5 87 1.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3366539831864217 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010197773061307736 16-17 0.001593402040829334 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.549443265326934E-4 26-27 0.0012747216326634671 28-29 0.0 30-31 0.004206581387789441 32-33 0.0 34-35 0.010580189551106777 36-37 0.02931859755125974 38-39 0.02313619763284193 40-41 0.01325710497970006 42-43 0.01045271738784043 44-45 0.0026131793469601075 46-47 0.0010197773061307736 48-49 6.373608163317336E-4 50-51 0.0 52-53 1.274721632663467E-4 54-55 0.0 56-57 0.0 58-59 0.004334053551055789 60-61 0.010325245224574084 62-63 0.004142845306156268 64-65 0.0018483463673620274 66-67 0.0036966927347240547 68-69 0.002995595836759148 70-71 6.373608163317335E-5 72-73 0.0 74-75 0.0 76-77 0.2202718981242471 78-79 5.736247346985602E-4 80-81 0.0035054844898245348 82-83 5.736247346985602E-4 84-85 0.0 86-87 0.11172935110295289 88-89 0.4068274090645455 90-91 0.3926779989419811 92-93 0.0 94-95 0.0 96-97 6.373608163317335E-5 98-99 0.0016571381224625074 100-101 5.098886530653868E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 784485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.723232548101855 #Duplication Level Percentage of deduplicated Percentage of total 1 74.13777213746387 40.57058545276621 2 14.147151991358283 15.48355776632883 3 5.084197382812629 8.346711471003188 4 2.345344283116179 5.133792824413115 5 1.2495951530288003 3.419094307508798 6 0.7343223620384416 2.4110696029860645 7 0.462529453936412 1.7717774807676185 8 0.3288457247596007 1.439640085477498 9 0.25223035134261 1.2422574154989974 >10 1.1729101726480051 11.237623484774497 >50 0.05766447296198554 2.121486866789712 >100 0.021158667991379814 2.3813965032079345 >500 0.003022666853472824 1.1629602495397064 >1k 0.003022666853472824 2.5730177424717664 >5k 2.3251283488252492E-4 0.70502874646603 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5541 0.7063232566588271 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2505 0.31931776898219855 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2472 0.31511118759440904 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2006 0.2557091595122915 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1959 0.24971796783877323 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1953 0.2489531348591751 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1490 0.1899335232668566 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1236 0.15755559379720452 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1176 0.14990726400122373 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1115 0.14213146204197658 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1114 0.14200398987871024 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1095 0.13958201877664964 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1064 0.1356303817153929 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1037 0.13218863330720154 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 997 0.12708974677654766 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 960 0.12237327673569284 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 876 0.11166561502131973 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 847 0.10796892228659566 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.8241648979904015E-4 0.0 10-11 0.0 0.0 0.0 5.736247346985602E-4 0.0 12-13 0.0 0.0 0.0 8.92305142864427E-4 0.0 14-15 0.0 0.0 0.0 8.92305142864427E-4 0.0 16-17 0.0 0.0 0.0 8.92305142864427E-4 0.0 18-19 0.0 0.0 0.0 0.0010197773061307736 0.0 20-21 0.0 0.0 0.0 0.001784610285728854 0.0 22-23 0.0 0.0 0.0 0.0020395546122615473 0.0 24-25 0.0 0.0 0.0 0.002676915428593281 0.0 26-27 0.0 0.0 0.0 0.0028043875918596275 0.0 28-29 0.0 0.0 0.0 0.004142845306156268 0.0 30-31 0.0 0.0 0.0 0.011727439020503896 0.0 32-33 0.0 0.0 0.0 0.028744972816561184 0.0 34-35 0.0 0.0 0.0 0.056597640490257936 0.0 36-37 0.0 0.0 0.0 0.10395354914370575 0.0 38-39 0.0 0.0 0.0 0.17521048840959355 0.0 40-41 0.0 0.0 0.0 0.2653333078389007 0.0 42-43 0.0 0.0 0.0 0.3559660159212732 0.0 44-45 0.0 0.0 0.0 0.4752162246569405 0.0 46-47 0.0 0.0 0.0 0.6320069854745469 0.0 48-49 0.0 0.0 0.0 0.8053053914351453 0.0 50-51 0.0 0.0 0.0 0.992307054946876 0.0 52-53 0.0 0.0 0.0 1.175739497887149 0.0 54-55 0.0 0.0 0.0 1.3858773590317215 0.0 56-57 0.0 0.0 0.0 1.653441429727783 0.0 58-59 0.0 0.0 0.0 1.9434406011587222 0.0 60-61 0.0 0.0 0.0 2.2756330586308215 0.0 62-63 0.0 0.0 0.0 2.618087025245862 0.0 64-65 0.0 0.0 0.0 2.9772398452487936 0.0 66-67 0.0 0.0 0.0 3.399810066476733 0.0 68-69 0.0 0.0 0.0 3.8852878002766147 0.0 70-71 0.0 0.0 0.0 4.406266531546174 0.0 72-73 0.0 0.0 0.0 4.937060619387242 0.0 74-75 0.0 0.0 0.0 5.513489741677661 0.0 76-77 0.0 0.0 0.0 6.134789065437835 0.0 78-79 0.0 0.0 0.0 6.87259794642345 0.0 80-81 0.0 0.0 0.0 7.645780352715476 0.0 82-83 0.0 0.0 0.0 8.42316934039529 0.0 84-85 0.0 0.0 0.0 9.247021931585689 0.0 86-87 0.0 0.0 0.0 10.135885326041926 0.0 88-89 0.0 0.0 0.0 11.09747158964161 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGTG 70 2.0660655E-4 33.891216 7 TATAGGG 135 1.2090823E-8 31.631802 2 ACAGCGT 175 5.014954E-9 27.112972 8 GTACATG 4525 0.0 27.0309 1 TACATGG 4570 0.0 26.682842 2 ACATGGG 4465 0.0 25.928865 3 GTATAGG 170 1.10076144E-7 25.196459 1 ATGGGAG 1050 0.0 23.046026 5 CATGGGG 1550 0.0 22.958565 4 CATGGGA 2945 0.0 22.072445 4 GTGTAGC 560 0.0 21.247015 1 CCGTCTG 115 0.0037460718 20.629435 9 GTATATA 255 1.1637894E-8 20.530447 1 CGGGAGT 285 1.9881554E-9 19.97798 4 GTATAGC 310 2.5283953E-10 19.958487 1 ACCTAAT 120 0.0047084037 19.830547 1 GTATCGT 145 6.1893975E-4 19.633532 6 GTATAGA 270 2.2448148E-8 19.389868 1 ATGCACT 570 0.0 19.145563 4 GTCGGGA 250 2.1951382E-7 18.97908 2 >>END_MODULE