FastQCFastQC Report
Fri 27 May 2016
SRR939114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771136
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50810.6588980413312309No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25730.3336635820400033No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23920.3101917171549506No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22430.290869574238526No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21670.2810139845630343No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18530.24029483774587104No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13960.18103162088140096No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13300.17247281932110547No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA11450.14848223918997427No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11230.14562930533654245No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11020.14290605029463027No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10050.13032720557722632No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9870.12799298696987302No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9720.1260478047970786No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9100.11800771848286165No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8360.10841148643040915No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8020.1040024068387418No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT753.140346E-431.5577286
ACACGGC953.5724122E-529.8967938
GTACATG42300.028.7339251
TACATGG42200.028.0575712
GTACGCT700.00851051327.0635283
GGATTCG700.008532098527.0494798
ACATGGG43000.026.3240053
GTCGGGA2351.8189894E-1024.1844312
GTAAGCG801.8403189E-623.77940894-95
CATGGGA28650.023.4739514
TAGCCCT6100.023.2923834
ATGGGAT9700.022.9467125
CATGGGG14050.021.9108664
GTGTAGC4850.021.5365621
GTCCTAC1353.8060767E-421.1014771
GTATATG2701.0059011E-921.1014771
GTATAGC3401.8189894E-1220.946321
ATAGCCC2751.3042154E-920.6666953
CGGGAGT3102.6921043E-1019.861144
ACCGCTC1456.2877446E-419.5875558
Produced by FastQC (version 0.11.5)