FastQCFastQC Report
Fri 27 May 2016
SRR939114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771136
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54140.702081085567267No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25200.326790605029463No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24920.32315959830691343No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20350.2638963814424434No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19480.2526143248402357No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18710.24262905635322432No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14570.18894202838409827No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12190.15807847124242677No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12030.1560036102581127No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11920.1545771433313968No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10900.14134990455639473No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10730.13914536476056102No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10640.13797825545688439No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG9930.1287710598389908No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9830.1274742717237945No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9280.12034193709021496No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT8160.10581791020001659No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7850.10179786704290814No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT300.003955702831.6595780-81
CTAGCGT600.00397084631.6328854
GTCGGGA2000.030.8420622
GTAAGCG1550.027.5512294-95
GTACATG43500.026.2897421
GTATTAC1109.2715185E-525.9909951
TACATGG44600.025.3204922
GGTCGGG2950.024.2288951
ACATGGG44250.024.0195473
GTCTTAG2853.6379788E-1223.4070971
ATTCGCG1253.8380676E-1022.79489180-81
GCCTTAC1050.002155655822.6905541
CATGGGG15950.022.6090834
CGCGGAA1205.5897544E-921.88311882-83
AATTCGC1451.07320375E-1021.27176778-79
CGGGAGT3401.8189894E-1220.9335274
TATACTG3201.8189894E-1120.759085
GTAGGAC1150.003745270420.6301423
CGATCGT1501.6916601E-1020.56404124-25
GTATAGC2651.7864295E-819.7793121
Produced by FastQC (version 0.11.5)