FastQCFastQC Report
Fri 27 May 2016
SRR939113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584754
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104590.6599762486796058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82490.5205224280866305No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53430.3371501191983109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49220.31058448188173055No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40170.2534778268425257No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT37960.2395324447832282No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT21330.13459502231892143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.1273383755459838No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20060.12658116023054683No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA18630.1175576777215896No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17640.1113106513692346No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG15970.10077273822940343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT81800.023.96731212-13
GTATCAA209250.022.1446741
GTACTTT89050.021.8585514-15
GTACATG134900.021.433361
AGTACTT84800.021.10902812-13
TACATGG137800.020.7302742
CGCGGAA1401.5588739E-920.37769582-83
GGTATCA160200.020.330431
TCGCGGA1452.41198E-919.67501682-83
ACTTTTT101950.019.56036416-17
ATCAACG238200.019.2716523
ACATGGG138400.019.2712213
TCAACGC239550.019.1828214
TATCAAC239900.019.1745832
CAACGCA240250.019.1663685
AACGCAG243900.018.9112156
ATGGGAG32850.018.6033555
CATGGGA95350.017.9855964
AAGGGTA2905.378024E-817.969324
TACTTTT100250.017.73940314-15
Produced by FastQC (version 0.11.5)