##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939113_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1584754 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24088470513404 33.0 31.0 34.0 31.0 34.0 2 32.32894190517897 34.0 31.0 34.0 31.0 34.0 3 32.36011772174104 34.0 31.0 34.0 30.0 34.0 4 35.811124628806745 37.0 35.0 37.0 35.0 37.0 5 35.87063796652351 37.0 35.0 37.0 35.0 37.0 6 35.973708853235266 37.0 37.0 37.0 35.0 37.0 7 35.91579576388512 37.0 37.0 37.0 35.0 37.0 8 35.91330768056115 37.0 37.0 37.0 35.0 37.0 9 37.601764690292626 39.0 38.0 39.0 35.0 39.0 10-11 37.53032962844706 39.0 38.0 39.0 35.0 39.0 12-13 37.45397172053202 39.0 38.0 39.0 35.0 39.0 14-15 38.736239820186604 40.0 38.0 41.0 35.0 41.0 16-17 38.693823457773256 40.0 38.0 41.0 35.0 41.0 18-19 38.70240396932269 40.0 38.5 41.0 35.0 41.0 20-21 38.64417979068044 40.0 38.5 41.0 34.5 41.0 22-23 38.56401971536276 40.0 38.0 41.0 34.0 41.0 24-25 38.584887938443444 40.0 38.0 41.0 34.0 41.0 26-27 38.490664797186184 40.0 38.0 41.0 34.0 41.0 28-29 38.375929008540126 40.0 38.0 41.0 34.0 41.0 30-31 38.18454441509534 40.0 38.0 41.0 34.0 41.0 32-33 37.95808813229056 40.0 38.0 41.0 33.5 41.0 34-35 37.66135879764305 40.0 37.0 41.0 33.0 41.0 36-37 37.40084770254563 40.0 37.0 41.0 33.0 41.0 38-39 37.152651452528275 40.0 36.5 41.0 31.5 41.0 40-41 36.9806001436185 40.0 36.5 41.0 31.0 41.0 42-43 36.74665910292701 40.0 36.0 41.0 31.0 41.0 44-45 36.41168534674782 40.0 36.0 41.0 30.0 41.0 46-47 35.94499430195475 39.5 35.5 41.0 28.5 41.0 48-49 35.804176862781226 40.0 36.0 41.0 27.5 41.0 50-51 34.78107422350725 38.5 34.5 40.0 25.5 40.5 52-53 34.912985548545706 38.5 35.0 40.0 26.0 40.5 54-55 35.41463406938868 39.0 35.0 41.0 26.0 41.0 56-57 35.33009192593929 39.0 35.0 41.0 26.0 41.0 58-59 35.16807214242715 39.0 35.0 41.0 26.0 41.0 60-61 34.89898463736328 39.0 35.0 41.0 25.5 41.0 62-63 34.559235061088344 38.5 35.0 40.5 23.5 41.0 64-65 34.20377168948619 38.0 34.0 40.0 23.0 41.0 66-67 33.837827195892864 37.0 34.0 40.0 22.0 41.0 68-69 33.45442857377233 37.0 34.0 39.5 20.5 41.0 70-71 33.05820493275297 36.0 34.0 39.0 19.5 41.0 72-73 32.61814199554 35.5 33.0 39.0 18.0 40.5 74-75 32.1723466228828 35.0 33.0 37.5 14.5 40.0 76-77 31.737264584913493 35.0 32.5 37.0 10.0 39.0 78-79 31.321753723290808 35.0 32.0 37.0 7.0 39.0 80-81 30.910251370244215 35.0 32.0 36.0 3.5 38.5 82-83 30.499328602420313 35.0 31.0 36.0 2.0 37.0 84-85 30.12087648934787 35.0 31.0 35.0 2.0 37.0 86-87 29.743488579300006 34.5 31.0 35.0 2.0 36.5 88-89 29.35574606531992 34.0 30.5 35.0 2.0 36.0 90-91 28.994409542427405 34.0 30.0 35.0 2.0 36.0 92-93 28.62371131418504 34.0 29.5 35.0 2.0 35.5 94-95 28.220521292263655 34.0 29.0 35.0 2.0 35.0 96-97 27.71368994809289 34.0 29.0 35.0 2.0 35.0 98-99 27.103366516191155 34.0 27.0 35.0 2.0 35.0 100-101 24.745445034371265 31.5 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17929383497303775 1101 2 0.3263162241061224 1101 3 0.2653098928920272 1101 4 0.03137018130702529 1101 5 0.18535786673432852 1101 6 0.2080016075613642 1101 7 0.11555646900637129 1101 8 0.2424663851278197 1101 9 0.2732200805521643 1101 10-11 0.31294756425176473 1101 12-13 0.28556434253921026 1101 14-15 0.3812861994062047 1101 16-17 0.38130962339081265 1101 18-19 0.4149551544833585 1101 20-21 0.49723207208769793 1101 22-23 0.43184673842331023 1101 24-25 0.5660806910798826 1101 26-27 0.37824522397566085 1101 28-29 0.34624280504387883 1101 30-31 0.2962661317794115 1101 32-33 0.26627714705063 1101 34-35 0.35611611347413685 1101 36-37 0.11959753410933871 1101 38-39 0.016215116949297226 1101 40-41 -0.07482181271740984 1101 42-43 -0.038325622511976576 1101 44-45 -0.2413340962612338 1101 46-47 -0.33902723102979593 1101 48-49 -0.15603847141094462 1101 50-51 -0.1866303796777018 1101 52-53 -0.2088204377856755 1101 54-55 -0.17382356380307584 1101 56-57 -0.22438587207917493 1101 58-59 -0.2799071037168872 1101 60-61 -0.29246849055502366 1101 62-63 -0.2471924859195198 1101 64-65 -0.10665361301371945 1101 66-67 -0.2965666462717138 1101 68-69 -0.2456603735958467 1101 70-71 -0.3240903942244202 1101 72-73 -0.4978667398944978 1101 74-75 -0.44995563841691677 1101 76-77 -0.4894783763042021 1101 78-79 -0.45733483164221056 1101 80-81 -0.48575484376490863 1101 82-83 -0.49955573510717244 1101 84-85 -0.5431531349952614 1101 86-87 -0.520546588663116 1101 88-89 -0.54549414827747 1101 90-91 -0.6510354587980203 1101 92-93 -0.747005480754563 1101 94-95 -0.7096392278788421 1101 96-97 -0.7054497353953018 1101 98-99 -0.9099852437972018 1101 100-101 -1.0535244194346092 1102 1 0.0505759286825338 1102 2 0.22784624258327568 1102 3 0.2992554542389243 1102 4 0.29873180950260547 1102 5 0.35555173893862246 1102 6 0.25596939664605145 1102 7 0.1903293655067415 1102 8 0.26654514743442803 1102 9 0.41865622406724157 1102 10-11 0.2754739700320812 1102 12-13 0.2799712811233732 1102 14-15 0.32489007133472825 1102 16-17 0.34883892484416634 1102 18-19 0.4620258364832992 1102 20-21 0.4818585302659031 1102 22-23 0.5628312672559375 1102 24-25 0.4082298320302087 1102 26-27 0.40581644374346126 1102 28-29 0.34951739758131595 1102 30-31 0.373481045874442 1102 32-33 0.31613760318807493 1102 34-35 0.2314709023657926 1102 36-37 0.12744315336969692 1102 38-39 0.05381446611231411 1102 40-41 -0.026993843273650953 1102 42-43 -0.181058586302278 1102 44-45 -0.08799745358729183 1102 46-47 2.6098495983717385E-4 1102 48-49 -0.09316127552555287 1102 50-51 0.03460951993896799 1102 52-53 -0.08050296170945614 1102 54-55 0.018689880393168323 1102 56-57 0.05536703048831981 1102 58-59 -0.07877227566471134 1102 60-61 -0.2413893697365168 1102 62-63 -0.24639438327480434 1102 64-65 -0.14086805828549842 1102 66-67 -0.204563667865159 1102 68-69 -0.1784291885761249 1102 70-71 -0.3237652515091085 1102 72-73 -0.4988634029760419 1102 74-75 -0.3670202532924023 1102 76-77 -0.35611443157019096 1102 78-79 -0.26241163592228745 1102 80-81 -0.3434396842271106 1102 82-83 -0.4476341113240423 1102 84-85 -0.7018666288316133 1102 86-87 -0.7589164626323956 1102 88-89 -0.748103563351517 1102 90-91 -0.8680661529322151 1102 92-93 -0.8241473135777078 1102 94-95 -1.0500493103365791 1102 96-97 -1.0685431415341249 1102 98-99 -1.1291962418391748 1102 100-101 -1.1171522840825752 1103 1 0.2096722979225163 1103 2 0.21297102165676307 1103 3 0.2557849272854824 1103 4 0.20549011332936118 1103 5 0.22987784191708727 1103 6 0.2519432330907563 1103 7 0.18082959355115946 1103 8 0.19702169790600266 1103 9 0.3192738692279491 1103 10-11 0.3560775040969304 1103 12-13 0.3121239174090604 1103 14-15 0.32036850093570735 1103 16-17 0.37553367993097453 1103 18-19 0.34066878508409815 1103 20-21 0.40076691064074765 1103 22-23 0.3795128386094362 1103 24-25 0.2798831242486628 1103 26-27 0.29598038083682354 1103 28-29 0.36391537929329587 1103 30-31 0.32747084705550833 1103 32-33 0.3741077963452284 1103 34-35 0.4373424089611788 1103 36-37 0.3171479104142705 1103 38-39 0.4004720833111435 1103 40-41 0.27031832377742404 1103 42-43 0.047656951467679676 1103 44-45 0.18787040321274873 1103 46-47 0.39635163654863703 1103 48-49 0.16789101524979344 1103 50-51 0.3678510208779926 1103 52-53 0.3654591877976685 1103 54-55 0.2684771564364681 1103 56-57 0.31533034814687966 1103 58-59 0.14561014696423769 1103 60-61 0.1510043048915506 1103 62-63 0.003389910023400944 1103 64-65 0.03977755960099927 1103 66-67 0.11105170769769757 1103 68-69 -0.0813386666011624 1103 70-71 -0.09493040393551411 1103 72-73 -0.0399762055145203 1103 74-75 0.13257572032711806 1103 76-77 0.13002231156501765 1103 78-79 0.17803270632547452 1103 80-81 0.13603550448574708 1103 82-83 0.10987023128165063 1103 84-85 -0.02551697735182401 1103 86-87 0.07075577706750735 1103 88-89 -0.08837186940719377 1103 90-91 -0.13013637325946092 1103 92-93 -0.00995064004349544 1103 94-95 0.15337471833248273 1103 96-97 -0.02096401879741805 1103 98-99 -0.39269167200254174 1103 100-101 -0.5987433236993986 1104 1 0.0037725485620754284 1104 2 0.12107831716002693 1104 3 0.20263689961404907 1104 4 0.17537967122398612 1104 5 0.17220378738807796 1104 6 0.16019878694715572 1104 7 0.13811874658644285 1104 8 0.18302787188147818 1104 9 0.22933185068529127 1104 10-11 0.21543734941058545 1104 12-13 0.15736215458613145 1104 14-15 0.2165886905094041 1104 16-17 0.148920009577715 1104 18-19 0.18965002729211022 1104 20-21 0.24553574943052325 1104 22-23 0.12277991359136564 1104 24-25 0.14021934931106017 1104 26-27 0.23212949971047436 1104 28-29 0.24523845549860113 1104 30-31 0.18447359190858492 1104 32-33 0.20370037098631144 1104 34-35 0.15986990285070846 1104 36-37 0.2899059564106423 1104 38-39 0.2530452210747782 1104 40-41 0.09108434254817865 1104 42-43 0.10778219348907925 1104 44-45 0.08909690413417337 1104 46-47 0.1793739815707056 1104 48-49 0.33908871983724254 1104 50-51 0.3419527229979309 1104 52-53 0.3734703227257228 1104 54-55 0.2834204061455168 1104 56-57 0.24928488052395892 1104 58-59 0.2428357458942898 1104 60-61 0.25761191013035756 1104 62-63 0.33023996432432057 1104 64-65 0.3024211200823643 1104 66-67 0.2547500797069375 1104 68-69 0.2208336293038542 1104 70-71 0.1924813732726065 1104 72-73 0.10204363311196119 1104 74-75 -0.024894349369859015 1104 76-77 0.09944609977329932 1104 78-79 0.02995526984718211 1104 80-81 -0.138485830979878 1104 82-83 -0.08767884041888863 1104 84-85 -0.03152403258324199 1104 86-87 0.005298936577425195 1104 88-89 0.13114483946472077 1104 90-91 -0.007164363503441962 1104 92-93 0.09301297186175717 1104 94-95 -0.026783026481712824 1104 96-97 0.04517501747144692 1104 98-99 -0.021421911781914105 1104 100-101 0.07589827728346066 1105 1 0.1357458348709386 1105 2 0.12810451825830427 1105 3 0.1142340065301255 1105 4 0.12886616109108218 1105 5 0.06597149207572528 1105 6 0.1821578727169424 1105 7 0.10035316094296576 1105 8 0.04653101488507616 1105 9 0.049788475273558674 1105 10-11 0.14060639468799252 1105 12-13 0.17447441732799263 1105 14-15 0.26675828762122933 1105 16-17 0.1253701691191722 1105 18-19 0.09480072714078602 1105 20-21 0.2156837146330659 1105 22-23 0.0714416538073337 1105 24-25 0.16447234945085398 1105 26-27 0.18307509864702354 1105 28-29 0.09684086002511805 1105 30-31 0.10647952228914903 1105 32-33 0.16922862368232217 1105 34-35 0.11650307521190228 1105 36-37 0.14837538846266085 1105 38-39 0.23187047596096733 1105 40-41 0.06566954700895877 1105 42-43 0.059117264896450195 1105 44-45 0.18034137659272886 1105 46-47 0.23244352987583738 1105 48-49 0.3757768794326637 1105 50-51 0.1130010367145644 1105 52-53 0.24885728128418805 1105 54-55 0.410935606118052 1105 56-57 0.4331386959071395 1105 58-59 0.27414118992479075 1105 60-61 0.5494607025940184 1105 62-63 0.41503484133882296 1105 64-65 0.36119614577332726 1105 66-67 0.2654044081734952 1105 68-69 0.2937272773217856 1105 70-71 0.25658270478775336 1105 72-73 0.3865174651237524 1105 74-75 0.5023822128733855 1105 76-77 0.4006579281601468 1105 78-79 0.4312674094686457 1105 80-81 0.3435692494530542 1105 82-83 0.2462545617506784 1105 84-85 0.266817766298864 1105 86-87 0.3540428388070822 1105 88-89 0.2776867997277357 1105 90-91 0.24009326468769387 1105 92-93 0.13423402201491896 1105 94-95 -0.11780651991243118 1105 96-97 -0.03920193694212415 1105 98-99 -0.013417614831226388 1105 100-101 0.04307639226613347 1106 1 0.08191982769923101 1106 2 0.112654827238309 1106 3 0.20628072344201343 1106 4 0.16136350128030585 1106 5 0.20741679941450286 1106 6 0.217463494297796 1106 7 0.19316501944057052 1106 8 0.184287805746834 1106 9 0.16785230796996586 1106 10-11 0.20297279990826667 1106 12-13 0.21057510480448371 1106 14-15 0.3168506616640556 1106 16-17 0.3394065580007748 1106 18-19 0.3898820453020235 1106 20-21 0.3089087587944377 1106 22-23 0.3859839016386317 1106 24-25 0.3251774030222805 1106 26-27 0.31672811568500947 1106 28-29 0.10218580214920081 1106 30-31 0.18023926322749162 1106 32-33 0.16660149945915492 1106 34-35 0.11020354760165674 1106 36-37 0.11507515601982021 1106 38-39 0.1892849603397906 1106 40-41 0.0808948057046237 1106 42-43 0.07189333391940522 1106 44-45 -0.03963291612318898 1106 46-47 -0.0881558586554192 1106 48-49 -0.007291347533552539 1106 50-51 0.053764484123973943 1106 52-53 -0.16402269415026183 1106 54-55 -0.14173048876410377 1106 56-57 -0.1080673090271489 1106 58-59 0.05535972623932395 1106 60-61 -0.033339862929402386 1106 62-63 0.01265461569110471 1106 64-65 -0.07662689290683744 1106 66-67 -0.038557294234301764 1106 68-69 -0.0035903075377987648 1106 70-71 -0.00890669348962092 1106 72-73 0.04930018655098678 1106 74-75 -0.1323381812804172 1106 76-77 -0.027062433307097677 1106 78-79 0.06075542294103187 1106 80-81 0.16874452534368345 1106 82-83 0.050647634595193836 1106 84-85 0.023110503392626214 1106 86-87 -0.07482477466475146 1106 88-89 -0.026660926277916275 1106 90-91 0.22165436529460436 1106 92-93 -0.02146699637840399 1106 94-95 -0.036903385824363255 1106 96-97 0.12409156943658672 1106 98-99 0.3123980412914946 1106 100-101 0.2921647366553479 1107 1 0.06215563308106198 1107 2 0.07909898717122132 1107 3 0.005026825845988014 1107 4 0.08788145292007243 1107 5 0.029680718805046524 1107 6 -0.006780134127801318 1107 7 -0.024548586804527872 1107 8 0.06514384919726979 1107 9 0.08857622461481185 1107 10-11 0.12347916610969634 1107 12-13 0.05270497627866888 1107 14-15 0.06208724996431414 1107 16-17 0.15705389031039374 1107 18-19 0.17329347310401033 1107 20-21 0.09838330868213774 1107 22-23 0.1369105744195238 1107 24-25 0.04327895960447137 1107 26-27 0.11472682704951609 1107 28-29 0.08268258034117082 1107 30-31 0.0487719074171622 1107 32-33 0.053864754355899436 1107 34-35 0.004595965617816944 1107 36-37 0.10169646166460211 1107 38-39 0.25457969952267945 1107 40-41 0.1425778127780859 1107 42-43 0.31050976573246913 1107 44-45 0.10839086178939539 1107 46-47 0.11581895461744551 1107 48-49 0.007330366895516249 1107 50-51 -0.047305460536634314 1107 52-53 0.04841114704932181 1107 54-55 0.059561567881466715 1107 56-57 0.0393314499703834 1107 58-59 0.08239804450420962 1107 60-61 -0.13302489725719369 1107 62-63 0.010482181525340195 1107 64-65 -0.2766798769664476 1107 66-67 -0.2071641511962028 1107 68-69 -0.04557842033594994 1107 70-71 -0.170684835021099 1107 72-73 -0.021310722287921635 1107 74-75 -0.012015899597336954 1107 76-77 -0.007267097395615707 1107 78-79 -0.13806462906931571 1107 80-81 -0.1626014671446896 1107 82-83 -6.736553912105592E-4 1107 84-85 -0.1892150011950129 1107 86-87 -0.23768211552287255 1107 88-89 -0.12606174147802207 1107 90-91 -0.19238294548365786 1107 92-93 -0.15910975837426733 1107 94-95 -0.24124619269001002 1107 96-97 -0.2445399812550768 1107 98-99 -0.18216758372084385 1107 100-101 -0.2175114333690935 1108 1 0.015198199881290009 1108 2 0.010575722932244958 1108 3 0.11234165945807462 1108 4 0.05732964659378581 1108 5 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-0.6267742682774795 2316 82-83 -0.6680372890681774 2316 84-85 -0.6388239329948284 2316 86-87 -0.7144427255044583 2316 88-89 -0.7923151963313941 2316 90-91 -0.9074498967193101 2316 92-93 -1.0644552886838206 2316 94-95 -0.9724296895734135 2316 96-97 -1.0967130535079335 2316 98-99 -1.2365957388323565 2316 100-101 -1.0818762350466962 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5131.0 3 1018.0 4 981.0 5 1057.0 6 1247.0 7 1427.0 8 1879.0 9 2245.0 10 2682.0 11 3928.0 12 5607.0 13 9351.0 14 8709.0 15 9778.0 16 13792.0 17 19907.0 18 17994.0 19 8976.0 20 8243.0 21 8157.0 22 8426.0 23 8996.0 24 9996.0 25 11425.0 26 13172.0 27 15341.0 28 18353.0 29 22100.0 30 26734.0 31 32875.0 32 41905.0 33 55004.0 34 78186.0 35 118847.0 36 191696.0 37 332086.0 38 387002.0 39 80478.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.631710186553605 20.255395186914527 15.449718162350138 20.663176464181735 2 15.051171100821506 21.814386537505364 40.36164407101977 22.772798290653366 3 18.697592202661067 27.884659728763488 31.049928011831458 22.367820056743987 4 12.42841014126223 18.230529295849575 38.84676595478522 30.49429460810298 5 13.018414033615516 38.87869081598414 33.24604765873661 14.856847491663732 6 28.01962482181215 37.57276727545805 19.28142532594876 15.12618257678104 7 26.729927809531873 35.26847666047867 21.182966106673117 16.818629423316338 8 26.429581210370124 33.456868420287975 20.44961549783145 19.663934871510456 9 27.178451671825826 17.491279119341062 21.44967153402233 33.88059767481079 10-11 23.950562389608717 27.762407167108204 27.259119231902464 21.02791121138062 12-13 26.69536113469157 25.361159956342473 27.7264797578624 20.216999151103565 14-15 23.789388247518076 25.996314420658194 27.63533924226131 22.57895808956242 16-17 20.868562680528203 29.707817070440377 27.963094012187263 21.460526236844157 18-19 20.743006286257152 27.82540476623613 31.391049158126656 20.040539789380066 20-21 22.64232113087473 26.36063972730035 30.731869560520714 20.265169581304203 22-23 21.888079592786966 26.648838145201996 30.471040656231107 20.992041605779928 24-25 21.40020339413569 26.836747298740743 31.10647610340055 20.656573203723017 26-27 21.447199692415882 26.6871238326194 31.094871186514574 20.77080528845015 28-29 20.823508524522218 26.824950338908515 31.58467532008027 20.766865816489 30-31 21.12817622944347 26.99999557771192 31.218637290881052 20.65319090196356 32-33 20.85131611198007 27.06807218716914 30.85547910417967 21.225132596671124 34-35 21.30048959802059 26.9249787427214 30.788928360913452 20.98560329834456 36-37 21.749962267118363 26.477416711798153 30.757688946612095 21.01493207447139 38-39 21.55230841508422 26.630701137612213 30.86484015543499 20.952150291868573 40-41 21.190684690286773 26.805998872335223 31.146842681255947 20.85647375612205 42-43 21.055181223045917 27.10852521727331 30.45133055630147 21.384963003379294 44-45 21.159311112104557 27.329295934605174 29.939813145864107 21.571579807426165 46-47 21.441419959990856 27.250324134950482 29.329290352938493 21.978965552120172 48-49 21.60325716413111 27.379529133150125 29.12649068407677 21.890723018641996 50-51 21.879974177084826 27.585113520629793 28.86887133821297 21.666040964072412 52-53 21.678090846566768 27.784649796064357 28.84093590652984 21.696323450839035 54-55 21.130650900406046 27.61768339834443 29.082581053030726 22.1690846482188 56-57 21.618852718839786 28.10400769892048 28.313280891411242 21.9638586908285 58-59 21.640692916460267 27.38043058332628 28.764450681567304 22.214425818646145 60-61 21.697357404409086 27.473186456646122 29.028636487326825 21.800819651617967 62-63 22.059524220885628 27.37715992692221 29.027623542256375 21.535692309935783 64-65 21.703238073372823 27.293800465576307 29.222698129164606 21.780263331886268 66-67 21.488846400597218 27.630501124699403 28.82617408437608 22.0544783903273 68-69 21.395342936300388 27.751951015669974 29.000537609682688 21.852168438346954 70-71 21.49204597351195 27.781942964428886 28.620477495306236 22.10553356675293 72-73 21.47100664747455 27.754617429309143 28.436616225520694 22.337759697695613 74-75 21.37398455202947 28.06611769444352 28.370827406748454 22.189070346778557 76-77 21.420434025911682 27.664546412388347 28.773787861060722 22.141231700639246 78-79 21.167478961387097 27.566463503166016 28.931904827420922 22.33415270802596 80-81 21.093088222613353 28.42225296603077 28.40560946289355 22.07904934846233 82-83 21.365280578865217 27.99016370469174 28.291555605156955 22.35300011128609 84-85 21.19881168279413 27.67403076688945 28.252970793014676 22.874186757301747 86-87 21.368747897457098 27.611457364631114 28.433387160545344 22.586407577366444 88-89 21.615033633321485 27.209298417126753 28.647668720741482 22.527999228810277 90-91 21.2503655139999 27.566479147148797 28.738375703356766 22.444779635494534 92-93 21.375194714917065 27.67270008067667 28.483399126256447 22.468706078149815 94-95 21.175895260322168 27.548174245184796 28.44104809448466 22.83488240000837 96-97 21.353534539870626 27.329347766451406 28.197637265631766 23.119480428046206 98-99 21.379814237480343 27.31614099279472 28.186730369720625 23.117314400004314 100-101 21.827852829404947 26.950349662545126 28.703529602014104 22.51826790603582 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2319.0 1 1367.5 2 459.0 3 498.0 4 438.0 5 448.0 6 825.0 7 1672.5 8 2711.5 9 3289.5 10 2984.5 11 2558.0 12 2624.5 13 2902.5 14 3138.0 15 3287.5 16 3350.0 17 3385.5 18 3473.5 19 3605.5 20 3845.5 21 4216.5 22 4766.5 23 5512.0 24 6389.5 25 7746.0 26 9888.5 27 12012.5 28 13659.0 29 15991.5 30 19529.0 31 23079.0 32 27213.0 33 32370.0 34 37818.0 35 41974.0 36 47189.0 37 53104.5 38 54550.5 39 53941.5 40 52745.5 41 52488.0 42 52952.5 43 53592.0 44 55912.0 45 58046.5 46 57365.0 47 57983.0 48 74280.0 49 73490.0 50 55712.0 51 49127.5 52 43641.0 53 41176.0 54 39263.5 55 36202.5 56 32458.5 57 29104.5 58 26711.5 59 23985.0 60 20734.5 61 17972.5 62 15287.0 63 11442.0 64 7943.0 65 6038.0 66 4474.5 67 3541.5 68 2690.5 69 1822.5 70 1414.5 71 1138.0 72 1043.0 73 971.0 74 820.5 75 685.0 76 481.0 77 310.0 78 222.5 79 134.0 80 87.5 81 73.0 82 58.0 83 39.5 84 27.0 85 24.0 86 17.5 87 8.0 88 6.0 89 9.0 90 6.0 91 4.0 92 3.0 93 3.0 94 2.5 95 2.5 96 3.5 97 1.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.345542588944404 2 0.1215961594039201 3 0.11705286751129827 4 0.12323679258736688 5 0.13951692186926173 6 0.09168615444415978 7 0.0997631178088208 8 0.10525292884573884 9 0.09370539528532504 10-11 0.09247492039773997 12-13 0.09604014250792237 14-15 0.09821713653980364 16-17 0.08704821063710835 18-19 0.10209786503141813 20-21 0.11149995519809383 22-23 0.11970312111532769 24-25 0.13330144615504994 26-27 0.13235492701075374 28-29 0.1278431857562751 30-31 0.1175576777215896 32-33 0.11093204371151612 34-35 0.334121257936563 36-37 0.49723805713694363 38-39 0.47344887597696556 40-41 0.45164738502000945 42-43 0.48275631422921156 44-45 0.48894023930528024 46-47 0.4962599873545043 48-49 0.507649767724202 50-51 0.49597603161121534 52-53 0.4990364435111065 54-55 0.48685789718782857 56-57 0.4663184317566007 58-59 0.44823991610054303 60-61 0.4038797188711939 62-63 0.47518416107484196 64-65 0.5047155583768838 66-67 0.511782901320962 68-69 0.5257282833802597 70-71 0.5170518578908777 72-73 0.5089117932499302 74-75 0.5156320791744334 76-77 0.493483531197902 78-79 0.4754681168181308 80-81 0.47717185127786393 82-83 0.48824612526612965 84-85 0.4880252707991272 86-87 0.49184289801445524 88-89 0.5028856213645777 90-91 0.517872174482601 92-93 0.5104893251570907 94-95 0.5148748638589964 96-97 0.5141807498198459 98-99 0.5088171413355006 100-101 0.4970172026699412 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1584754.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.66812431548053 #Duplication Level Percentage of deduplicated Percentage of total 1 73.76295663003548 34.42378829887895 2 13.841546732590004 12.919180472700873 3 4.958010028082304 6.941430850438324 4 2.364947398756169 4.414706368189008 5 1.255542204397441 2.929689983907612 6 0.8148583092768515 2.2816745326100634 7 0.5375774825179316 1.7561412948344925 8 0.35406738205404026 1.321892848140375 9 0.26545228717526476 1.1149344303951505 >10 1.5831430174945502 14.122298247117293 >50 0.16419697844035808 5.374680772763367 >100 0.09079072568316085 7.513793264993149 >500 0.0044717093212746925 1.3667687000897324 >1k 0.0020325951460339513 1.9980763359105715 >5k 2.710126861378602E-4 0.8595345473381595 >10k+ 1.355063430689301E-4 0.6614090516928937 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10459 0.6599762486796058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8249 0.5205224280866305 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5343 0.3371501191983109 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4922 0.31058448188173055 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4017 0.2534778268425257 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3796 0.2395324447832282 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2133 0.13459502231892143 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2018 0.1273383755459838 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2006 0.12658116023054683 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1863 0.1175576777215896 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1764 0.1113106513692346 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1597 0.10077273822940343 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.310127628641418E-5 0.0 10-11 0.0 0.0 0.0 6.310127628641418E-5 0.0 12-13 0.0 0.0 0.0 6.310127628641418E-5 0.0 14-15 0.0 0.0 0.0 1.2620255257282835E-4 0.0 16-17 0.0 0.0 0.0 1.2620255257282835E-4 0.0 18-19 0.0 0.0 0.0 1.2620255257282835E-4 0.0 20-21 0.0 0.0 0.0 3.155063814320709E-4 0.0 22-23 0.0 0.0 0.0 8.834178680097984E-4 0.0 24-25 0.0 0.0 0.0 0.0019245889267356323 0.0 26-27 0.0 0.0 0.0 0.0036598740246120216 0.0 28-29 0.0 0.0 0.0 0.008707976127525156 0.0 30-31 0.0 0.0 0.0 0.01949829437250198 0.0 32-33 0.0 0.0 0.0 0.03366453089880196 0.0 34-35 0.0 0.0 0.0 0.05455105334960505 0.0 36-37 0.0 0.0 0.0 0.09323213571317693 0.0 38-39 0.0 0.0 0.0 0.15166391755439645 0.0 40-41 0.0 0.0 0.0 0.22303146103433086 0.0 42-43 0.0 0.0 0.0 0.2954401755729911 0.0 44-45 0.0 0.0 0.0 0.3836557598213981 0.0 46-47 0.0 0.0 0.0 0.4937359363030477 0.0 48-49 0.0 0.0 0.0 0.6222101348221869 0.0 50-51 0.0 0.0 0.0 0.76557623454492 0.0 52-53 0.0 0.0 0.0 0.906228979387337 0.0 54-55 0.0 0.0 0.0 1.0595650807633237 0.0 56-57 0.0 0.0 0.0 1.2432528960330753 0.0 58-59 0.0 0.0 0.0 1.4688399587570058 0.0 60-61 0.0 0.0 0.0 1.7317514264043505 0.0 62-63 0.0 0.0 0.0 1.991286975770372 0.0 64-65 0.0 0.0 0.0 2.2511064808796823 0.0 66-67 0.0 0.0 0.0 2.5467990615578193 0.0 68-69 0.0 0.0 0.0 2.8855267126632906 0.0 70-71 0.0 0.0 0.0 3.282623044333695 0.0 72-73 0.0 0.0 0.0 3.692087226156236 0.0 74-75 0.0 0.0 0.0 4.108902706666145 0.0 76-77 0.0 0.0 0.0 4.540894044122936 0.0 78-79 0.0 0.0 0.0 5.037437987220729 0.0 80-81 0.0 0.0 0.0 5.587523363247545 0.0 82-83 0.0 0.0 0.0 6.171210168896876 0.0 84-85 0.0 0.0 0.0 6.776004351464012 0.0 86-87 0.0 0.0 0.0 7.4043353100859814 0.0 88-89 0.0 0.0 0.0 8.06629924896861 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 8180 0.0 23.967312 12-13 GTATCAA 20925 0.0 22.144674 1 GTACTTT 8905 0.0 21.85855 14-15 GTACATG 13490 0.0 21.43336 1 AGTACTT 8480 0.0 21.109028 12-13 TACATGG 13780 0.0 20.730274 2 CGCGGAA 140 1.5588739E-9 20.377695 82-83 GGTATCA 16020 0.0 20.33043 1 TCGCGGA 145 2.41198E-9 19.675016 82-83 ACTTTTT 10195 0.0 19.560364 16-17 ATCAACG 23820 0.0 19.271652 3 ACATGGG 13840 0.0 19.271221 3 TCAACGC 23955 0.0 19.182821 4 TATCAAC 23990 0.0 19.174583 2 CAACGCA 24025 0.0 19.166368 5 AACGCAG 24390 0.0 18.911215 6 ATGGGAG 3285 0.0 18.603355 5 CATGGGA 9535 0.0 17.985596 4 AAGGGTA 290 5.378024E-8 17.96932 4 TACTTTT 10025 0.0 17.739403 14-15 >>END_MODULE