FastQCFastQC Report
Fri 27 May 2016
SRR939113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584754
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104260.6578939065621541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89480.564630220210834No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA55340.349202462969016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52370.330461383911951No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44130.2784659322519457No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT34050.21485984575524025No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21460.1354153389106448No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20240.12771698320370228No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19310.12184856450906575No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG19280.12165926068020652No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA17250.10884970159406443No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG16300.1028550803468551No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT404.5230973E-429.65638292-93
CTAACGC650.005874757729.2001343
GAGTACT88900.023.77848212-13
GTATCAA211950.022.4022851
GTACTTT94300.022.26587314-15
ACCGTGC1503.0588148E-522.143438
AGTACTT93200.021.07766712-13
GGTATCA161550.020.960091
GTACATG135150.020.7553371
TCTATAC4000.020.166343
TACATGG138400.020.1251982
ACTTTTT106550.019.77277816-17
CGGTATA1456.191011E-419.6345712
TACCTGG11200.019.0648172
ATCAACG245900.019.0263983
AACGCTA1250.00606877418.9800875
ACATGGG136600.018.966193
TATCAAC248900.018.8733252
CAACGCA248900.018.8351975
TCAACGC248750.018.8274824
Produced by FastQC (version 0.11.5)