##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939113_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1584754 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50006183925076 34.0 31.0 34.0 31.0 34.0 2 32.702570872198464 34.0 31.0 34.0 31.0 34.0 3 32.774039377720456 34.0 31.0 34.0 31.0 34.0 4 36.24230448385049 37.0 35.0 37.0 35.0 37.0 5 36.21716367335246 37.0 37.0 37.0 35.0 37.0 6 36.2635494215506 37.0 37.0 37.0 35.0 37.0 7 36.22152523356937 37.0 37.0 37.0 35.0 37.0 8 36.21670366504833 37.0 37.0 37.0 35.0 37.0 9 37.94181305111077 39.0 38.0 39.0 35.0 39.0 10-11 37.891767428888016 39.0 38.0 39.0 35.0 39.0 12-13 37.83427680258261 39.0 38.0 39.0 35.0 39.0 14-15 39.14321055507669 40.5 38.5 41.0 36.0 41.0 16-17 39.09586093488327 40.0 38.0 41.0 36.0 41.0 18-19 39.188037386244176 40.5 38.5 41.0 36.0 41.0 20-21 39.277526038741655 41.0 39.0 41.0 36.0 41.0 22-23 39.23330087824357 41.0 39.0 41.0 36.0 41.0 24-25 39.170542557393766 41.0 39.0 41.0 35.5 41.0 26-27 39.018459331858445 40.5 39.0 41.0 35.0 41.0 28-29 38.863405613741946 40.0 38.0 41.0 35.0 41.0 30-31 38.64017475267455 40.0 38.0 41.0 35.0 41.0 32-33 38.45056172756151 40.0 38.0 41.0 34.0 41.0 34-35 38.37569805786892 40.0 38.0 41.0 34.0 41.0 36-37 38.243237120714 40.0 38.0 41.0 34.0 41.0 38-39 38.08275038270924 40.0 38.0 41.0 34.0 41.0 40-41 37.87680769381242 40.0 37.5 41.0 33.0 41.0 42-43 37.66281896117631 40.0 37.0 41.0 33.0 41.0 44-45 37.34948925826974 40.0 37.0 41.0 33.0 41.0 46-47 36.924280361494596 40.0 37.0 41.0 31.5 41.0 48-49 36.51430537484051 40.0 36.5 41.0 30.5 41.0 50-51 36.24058434305893 40.0 36.0 41.0 30.0 41.0 52-53 35.98668720823548 39.5 35.5 41.0 29.0 41.0 54-55 35.75347782684253 39.0 35.0 41.0 28.0 41.0 56-57 35.514942697731 39.0 35.0 41.0 28.0 41.0 58-59 35.24442216268267 39.0 35.0 40.0 27.5 41.0 60-61 34.90683033455035 38.0 35.0 40.0 26.5 41.0 62-63 34.53437852183998 38.0 34.0 40.0 26.0 41.0 64-65 34.135675000662566 37.0 34.0 40.0 25.5 41.0 66-67 33.80193929152411 37.0 34.0 39.5 25.0 41.0 68-69 33.36339488652497 36.0 33.5 39.0 23.5 40.5 70-71 32.94729434347539 36.0 33.0 39.0 22.0 40.0 72-73 32.50125792394277 35.0 33.0 38.0 21.0 40.0 74-75 32.02120045130033 35.0 32.0 37.0 19.5 39.5 76-77 30.02083761896168 33.5 29.5 35.5 14.0 37.5 78-79 30.974267930543164 34.5 31.0 36.0 15.5 39.0 80-81 31.010129332375875 35.0 31.0 36.0 16.0 37.5 82-83 30.724107022288635 35.0 31.0 35.5 10.5 37.0 84-85 30.35576247165175 34.5 31.0 35.0 7.0 37.0 86-87 29.8205541680286 34.0 30.5 35.0 2.0 36.0 88-89 29.28959952143992 34.0 30.0 35.0 2.0 36.0 90-91 28.844225980814688 34.0 29.0 35.0 2.0 35.5 92-93 28.598419691636686 34.0 29.0 35.0 2.0 35.0 94-95 28.318704354114267 34.0 29.0 35.0 2.0 35.0 96-97 27.81157517191943 34.0 29.0 35.0 2.0 35.0 98-99 27.106541456907507 34.0 27.0 35.0 2.0 35.0 100-101 23.57684946685732 29.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09100914678798944 1101 2 0.18130461426387257 1101 3 0.21123894401205945 1101 4 0.16162510860407053 1101 5 0.1891592782090541 1101 6 0.13901311928191262 1101 7 0.11742091716838132 1101 8 0.20408846556693305 1101 9 0.24746366534423458 1101 10-11 0.13677543541360393 1101 12-13 0.16465946569944379 1101 14-15 0.20065673222757852 1101 16-17 0.21530145396934586 1101 18-19 0.1746400854091874 1101 20-21 0.24186146414778165 1101 22-23 0.2112414300848755 1101 24-25 0.10200900762470866 1101 26-27 0.24480782930006484 1101 28-29 0.13086438690179847 1101 30-31 0.15444661750191102 1101 32-33 0.25173472684000586 1101 34-35 0.38333644264713485 1101 36-37 0.3577834262050885 1101 38-39 0.46692571319115217 1101 40-41 0.4052660661086449 1101 42-43 0.4561867300464044 1101 44-45 0.5901771502567712 1101 46-47 0.5482354588711331 1101 48-49 0.6370935617703424 1101 50-51 0.652304153021376 1101 52-53 0.5166416726209064 1101 54-55 0.3537239792598257 1101 56-57 0.45728393536972334 1101 58-59 0.47937254930895534 1101 60-61 0.4899582861975844 1101 62-63 0.4477509671952191 1101 64-65 0.4069682512176911 1101 66-67 0.6057968741344908 1101 68-69 0.5695151344356404 1101 70-71 0.33655676000471857 1101 72-73 0.3044458502074363 1101 74-75 0.2643140733933933 1101 76-77 0.36709062544433024 1101 78-79 0.4306797565728253 1101 80-81 0.2174475927688242 1101 82-83 0.3265702889194664 1101 84-85 0.25002446346373475 1101 86-87 0.28438224346881924 1101 88-89 0.5630682209195506 1101 90-91 0.5081091763981789 1101 92-93 0.44929080474737404 1101 94-95 0.33145610341612297 1101 96-97 0.18055699260554547 1101 98-99 0.3126511576908335 1101 100-101 0.10641473088482556 1102 1 0.06961481127600422 1102 2 0.13018522980122782 1102 3 0.054926652569463386 1102 4 0.025306750037010772 1102 5 0.07297605105040361 1102 6 0.1002761842828761 1102 7 0.14759603644253616 1102 8 0.07982272551564051 1102 9 0.17489865059009446 1102 10-11 0.15305077255268174 1102 12-13 0.1432204540891746 1102 14-15 0.15532428138507726 1102 16-17 0.1884489126715323 1102 18-19 0.22175985596157233 1102 20-21 0.19225306945375564 1102 22-23 0.3142857476712635 1102 24-25 0.27828213853671 1102 26-27 0.2635050522117339 1102 28-29 0.16679251905480186 1102 30-31 0.17894649340163227 1102 32-33 0.2991857072321622 1102 34-35 0.3127355215473244 1102 36-37 0.40215148003703405 1102 38-39 0.3971206885089842 1102 40-41 0.4505800397993909 1102 42-43 0.39899194777344604 1102 44-45 0.3660385440407268 1102 46-47 0.3659972415026118 1102 48-49 0.277914829799343 1102 50-51 0.2828601222445073 1102 52-53 0.33833495190167895 1102 54-55 0.4432794245040199 1102 56-57 0.47918432421725043 1102 58-59 0.2899381707755495 1102 60-61 0.2686436506387224 1102 62-63 0.3400482011380248 1102 64-65 0.19049793876077814 1102 66-67 0.16604590201568925 1102 68-69 0.260518967755182 1102 70-71 0.21293303203250957 1102 72-73 0.12843967277162704 1102 74-75 0.20506943038851233 1102 76-77 0.11222837674738173 1102 78-79 0.15916628479874362 1102 80-81 -0.00847033558395438 1102 82-83 -0.1861839098064344 1102 84-85 -0.12976787541274248 1102 86-87 -0.003484610727145565 1102 88-89 -0.1672125214758644 1102 90-91 0.14233989259464153 1102 92-93 0.013761172359959772 1102 94-95 -0.006381794049474365 1102 96-97 -0.10178932988969436 1102 98-99 -0.22631881004309662 1102 100-101 -0.6378192946522532 1103 1 0.13250843194349926 1103 2 0.1238731988717845 1103 3 0.1855735991333134 1103 4 0.10000173723561545 1103 5 0.15584159052865942 1103 6 0.10931227495616014 1103 7 0.1361412106592539 1103 8 0.11579842753181424 1103 9 0.22410092897349188 1103 10-11 0.18763082102500306 1103 12-13 0.2508346430618076 1103 14-15 0.3156576763618091 1103 16-17 0.22804187100692275 1103 18-19 0.32311760152690994 1103 20-21 0.2919450874652014 1103 22-23 0.2828847631484521 1103 24-25 0.19170823343464605 1103 26-27 0.25250333252360946 1103 28-29 0.38117298391834 1103 30-31 0.48773382313434155 1103 32-33 0.45262814757318637 1103 34-35 0.47097475831166946 1103 36-37 0.5503768280555974 1103 38-39 0.5288555235550447 1103 40-41 0.5048703389904148 1103 42-43 0.463905971501994 1103 44-45 0.5508906459811911 1103 46-47 0.657605586464129 1103 48-49 0.48717907335621646 1103 50-51 0.49209779225419936 1103 52-53 0.5044386028358971 1103 54-55 0.4282450334531305 1103 56-57 0.5724664230285441 1103 58-59 0.6393390999819886 1103 60-61 0.5036080393739226 1103 62-63 0.34420614779460834 1103 64-65 0.3985457646378805 1103 66-67 0.4224619222853363 1103 68-69 0.5468934085582688 1103 70-71 0.4063843880697746 1103 72-73 0.32110954959509996 1103 74-75 0.37283636844883006 1103 76-77 0.3863331600401061 1103 78-79 0.461610942675172 1103 80-81 0.4859294249287309 1103 82-83 0.6045293404691954 1103 84-85 0.5277172691609131 1103 86-87 0.7318819062780264 1103 88-89 0.6299854877858344 1103 90-91 0.7431780981968892 1103 92-93 0.6648328726710879 1103 94-95 0.5245655420608841 1103 96-97 0.6094296963362247 1103 98-99 0.6440155665279299 1103 100-101 0.3099466021588668 1104 1 -0.045310874300717785 1104 2 0.016274958273363893 1104 3 0.037167506026037245 1104 4 0.034950533673267614 1104 5 0.1142100229039329 1104 6 0.0910521333849843 1104 7 0.12322821913227244 1104 8 0.07209953713672235 1104 9 -0.012422982869814803 1104 10-11 0.08637606807473475 1104 12-13 0.07702271863209376 1104 14-15 0.21875347665883993 1104 16-17 0.09384369728027053 1104 18-19 0.12314015131138945 1104 20-21 0.14616487788180166 1104 22-23 0.1577723382172067 1104 24-25 0.07065010423733753 1104 26-27 0.016635956359507986 1104 28-29 -0.005417410910247611 1104 30-31 0.09225207420416126 1104 32-33 0.0011534694260078027 1104 34-35 -0.04737661916913538 1104 36-37 -0.02765086930038052 1104 38-39 0.13781012073036436 1104 40-41 0.12447128556297571 1104 42-43 0.1968773851142842 1104 44-45 0.153267963489931 1104 46-47 0.052152685706502666 1104 48-49 0.16146946792559902 1104 50-51 0.049658838667873795 1104 52-53 0.02290765390467442 1104 54-55 0.01742372883146004 1104 56-57 0.03754095395414225 1104 58-59 0.008256166021752165 1104 60-61 0.13356840877568033 1104 62-63 -0.058505787780390506 1104 64-65 -0.10664846158051944 1104 66-67 -0.02468552997780904 1104 68-69 -0.17008813431353786 1104 70-71 -0.1555666089158123 1104 72-73 -0.3223262599810468 1104 74-75 -0.050723490861258824 1104 76-77 0.16545361476817178 1104 78-79 0.1617262835268285 1104 80-81 0.18261564338171254 1104 82-83 0.16013296191274762 1104 84-85 0.2156432851323764 1104 86-87 0.202560706629491 1104 88-89 0.3805757996726804 1104 90-91 0.42539927788757126 1104 92-93 0.30621976217455327 1104 94-95 0.36454164447319215 1104 96-97 0.303791472637176 1104 98-99 0.47223597382368965 1104 100-101 0.16715151749349744 1105 1 0.06097979283030952 1105 2 0.07270824394455389 1105 3 0.06785685758379856 1105 4 0.04019467748376826 1105 5 0.12934254900569897 1105 6 0.01982379901747322 1105 7 0.09299995589008603 1105 8 0.0132622154712152 1105 9 0.05084422026166635 1105 10-11 0.08807645386830387 1105 12-13 0.019592206848592753 1105 14-15 0.09708575946294928 1105 16-17 -0.011895625949996713 1105 18-19 0.09356624106173683 1105 20-21 0.0854699589278809 1105 22-23 0.07003434429474709 1105 24-25 0.05945546855842565 1105 26-27 0.07737183369039258 1105 28-29 0.014649005427976647 1105 30-31 0.1401966749335699 1105 32-33 0.1401473158590747 1105 34-35 0.16211174448238808 1105 36-37 0.005492796498892005 1105 38-39 0.02078639675548999 1105 40-41 0.17335474058602074 1105 42-43 0.11977557043745435 1105 44-45 0.1392893844758234 1105 46-47 0.054323724945618324 1105 48-49 -0.029630791804464707 1105 50-51 -0.0703997293585914 1105 52-53 -0.01520252167676972 1105 54-55 0.08090038378288256 1105 56-57 -0.04130171437374486 1105 58-59 0.028545278206010494 1105 60-61 -0.06844664526730071 1105 62-63 0.07068355519328406 1105 64-65 0.05461840309251187 1105 66-67 -0.019816878832813245 1105 68-69 0.0028288133799208026 1105 70-71 0.10830957415587505 1105 72-73 0.010954260754438394 1105 74-75 0.09020962435441504 1105 76-77 0.07283838560973166 1105 78-79 0.3397475769776541 1105 80-81 0.18182541649192885 1105 82-83 0.1456130946473202 1105 84-85 0.23881763292540725 1105 86-87 0.3490788766975008 1105 88-89 0.4261646805769992 1105 90-91 0.5282965312237167 1105 92-93 0.4802099665004427 1105 94-95 0.4577577600003444 1105 96-97 0.4960834494552273 1105 98-99 0.47436262104943694 1105 100-101 0.23269571207276485 1106 1 -0.03431854563389436 1106 2 -0.005923174647286089 1106 3 -0.03493636957808377 1106 4 -0.04172381145827586 1106 5 -0.036815634579483 1106 6 0.041149243381966016 1106 7 0.06796942158462116 1106 8 -0.021251102458002435 1106 9 0.10421123455673609 1106 10-11 0.12507648195111898 1106 12-13 0.044515474012371214 1106 14-15 0.006609378260385768 1106 16-17 -0.03164428936918284 1106 18-19 0.013037621221556606 1106 20-21 0.0425331779457494 1106 22-23 0.0010484515351834034 1106 24-25 0.1017210242505584 1106 26-27 0.020814657771822453 1106 28-29 0.07623438615957667 1106 30-31 0.03322417261212962 1106 32-33 -0.04536614156346985 1106 34-35 0.05378251998699568 1106 36-37 0.09671981750621228 1106 38-39 0.2720099557503417 1106 40-41 0.06592738875393422 1106 42-43 -0.04577422890853455 1106 44-45 0.057031599896795626 1106 46-47 0.13418365155278877 1106 48-49 -0.12582041433000768 1106 50-51 0.11184323117769424 1106 52-53 0.24261658540944353 1106 54-55 0.10273385085486808 1106 56-57 0.1064778788121643 1106 58-59 0.12340580099751719 1106 60-61 -0.031927708013057554 1106 62-63 0.13281516967425944 1106 64-65 0.16222023667007335 1106 66-67 0.2529556361028753 1106 68-69 0.004130290674041248 1106 70-71 -0.0030825185524037124 1106 72-73 -0.1491932799046367 1106 74-75 -0.16663003704971402 1106 76-77 -0.02109908731452137 1106 78-79 -0.03625782228860075 1106 80-81 -0.08945732818419216 1106 82-83 0.0040630522238522815 1106 84-85 0.017748801027753558 1106 86-87 0.09013300424964399 1106 88-89 0.1847167030690322 1106 90-91 0.23108253051381666 1106 92-93 0.1201272925377701 1106 94-95 0.030883105568577207 1106 96-97 0.2991363735897359 1106 98-99 0.09058702943432095 1106 100-101 -0.28227449942765404 1107 1 0.008628368110429108 1107 2 -0.024771113466485417 1107 3 0.027526485657453748 1107 4 0.01042645098387851 1107 5 0.014306526573641065 1107 6 0.02308597743225249 1107 7 0.03507807531142504 1107 8 0.005868577918285212 1107 9 0.07334144069017157 1107 10-11 0.05119288635108177 1107 12-13 0.02373633639811601 1107 14-15 0.08955048481141858 1107 16-17 0.13241922834253472 1107 18-19 0.1912182328277794 1107 20-21 0.21671980903239074 1107 22-23 0.20779769087619826 1107 24-25 0.13041882256111847 1107 26-27 0.15869076016264216 1107 28-29 0.07721029288658343 1107 30-31 0.110522345232269 1107 32-33 0.03461500698883668 1107 34-35 0.1236841968359883 1107 36-37 0.14842883852482913 1107 38-39 0.2190061587056107 1107 40-41 0.14016867061728533 1107 42-43 0.060687106982769023 1107 44-45 0.14496934889007207 1107 46-47 0.15639090256169652 1107 48-49 0.06709331858559153 1107 50-51 0.03775173454906877 1107 52-53 -0.09937885217178177 1107 54-55 -0.07566562702407253 1107 56-57 0.08191441614597039 1107 58-59 0.034432494349204035 1107 60-61 0.12329672641061507 1107 62-63 0.02242060071580454 1107 64-65 -0.12604967149820112 1107 66-67 0.05071771749918952 1107 68-69 -0.031238981466209736 1107 70-71 0.010800577032505032 1107 72-73 -0.017184460339485952 1107 74-75 0.007146932516469917 1107 76-77 -0.042679564969329675 1107 78-79 0.15689385642448528 1107 80-81 -0.032524684169967344 1107 82-83 0.07047778144153583 1107 84-85 0.3048103392936028 1107 86-87 0.20068437619905666 1107 88-89 0.2850640381418934 1107 90-91 0.25179828901703516 1107 92-93 0.1539638428544272 1107 94-95 0.1577312426393931 1107 96-97 0.1405103011340323 1107 98-99 0.07986280137653878 1107 100-101 -0.3569949624592077 1108 1 0.04153475648565319 1108 2 -0.05912660608972686 1108 3 -0.005470365556234924 1108 4 0.007754187229913612 1108 5 0.029362430908975057 1108 6 0.022366097098021953 1108 7 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-1.0167835539055083 2316 92-93 -1.2008762605566758 2316 94-95 -0.8727227035709006 2316 96-97 -1.3068185238110672 2316 98-99 -1.2185099642922061 2316 100-101 -0.9663159352020045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 14.0 5 34.0 6 93.0 7 197.0 8 424.0 9 752.0 10 1398.0 11 2242.0 12 3637.0 13 5316.0 14 6605.0 15 9701.0 16 13487.0 17 20254.0 18 18934.0 19 10278.0 20 8024.0 21 7876.0 22 8042.0 23 8600.0 24 9768.0 25 11333.0 26 13544.0 27 16279.0 28 19581.0 29 23913.0 30 29460.0 31 36292.0 32 45710.0 33 59893.0 34 84193.0 35 125645.0 36 203208.0 37 361297.0 38 377909.0 39 40814.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16731995516689 20.68332499572566 15.696591290471698 20.452763758635754 2 15.298715131812257 22.266231856805536 39.759293871477844 22.675759139904365 3 18.689020504128717 27.968694194808783 31.23494245794615 22.107342843116346 4 12.657926719225824 18.8344689459689 38.475182899049315 30.032421435755957 5 12.906482646518008 39.09628876153649 33.20736215210689 14.789866439838612 6 27.914174692097323 38.07884378269435 18.847278505055044 15.159703020153287 7 26.67846239858047 35.499263608105736 21.004206331077253 16.818067662236537 8 26.43186261085317 33.98199341979891 20.134544541297892 19.45159942805003 9 26.974533586916326 17.580457282328993 21.299709607926527 34.14529952282815 10-11 23.97728606458794 28.081456175532605 26.97238814352259 20.968869616356862 12-13 26.67420306243114 25.616814975699697 27.620122744602632 20.088859217266528 14-15 23.641484651539642 26.222735592154024 27.525255791897134 22.6105239644092 16-17 20.684023826208385 30.027692493116316 27.895398919245007 21.39288476143029 18-19 20.55991673152954 27.9442512668374 31.442997286328755 20.052834715304304 20-21 22.523401108310818 26.4672308762117 30.871731511641553 20.137636503835925 22-23 21.81294383860208 26.66215071866044 30.66144650841708 20.863458934320406 24-25 21.35392674914466 26.92652324094839 31.263180345620057 20.456369664286893 26-27 21.386004600138193 26.831646931505897 31.2737776347465 20.508570833609404 28-29 20.719020112900804 26.860067871732774 31.810646952145255 20.61026506322117 30-31 21.013675152189784 27.174277260224184 31.328157587024414 20.483890000561622 32-33 20.83765051231926 27.101903513100456 30.994463494018632 21.06598248056165 34-35 21.255299941088097 26.914944406035836 30.99352852620223 20.836227126673837 36-37 21.678005193546067 26.533257504699314 31.009503406607696 20.779233895146927 38-39 21.46645236642816 26.693981216013945 31.09834973458097 20.74121668297692 40-41 21.090587799086467 26.844155536797597 31.323766773692224 20.74148989042371 42-43 21.023837584466524 27.15518805486005 30.57142992374939 21.24954443692404 44-45 21.08026926274573 27.31505539700795 30.079683977541453 21.52499136270487 46-47 21.3924386512407 27.300919620086823 29.399541436225274 21.9071002924472 48-49 21.597567047233586 27.410851363093403 29.230738650669103 21.760842939003915 50-51 21.913761955333104 27.63578929965742 28.862163378141574 21.588285366867897 52-53 21.67318818768603 27.920944147132076 28.831054429302235 21.57481323587966 54-55 21.158678255426395 27.755822039256568 28.99803376423091 22.087465941086123 56-57 21.588997409061594 28.192703725625556 28.244446772180414 21.973852093132436 58-59 21.661731367206787 27.435164048091952 28.773642503419477 22.12946208128178 60-61 21.71779164334294 27.59597016498368 28.977344799666284 21.7088933920071 62-63 22.07126948072334 27.37658277672621 28.94611546654189 21.60603227600856 64-65 21.654469972278996 27.405394899663598 29.10932474463927 21.830810383418132 66-67 21.40926830361073 27.70545860821787 28.80975060903444 22.07552247913696 68-69 21.23217198069401 27.831792915899296 28.971290590364863 21.96474451304183 70-71 21.422486658804242 27.86020913038183 28.580952362923433 22.13635184789049 72-73 21.324129801849374 27.753487292033967 28.360742424376273 22.561640481740383 74-75 21.209159276455523 28.04693977740394 28.31209134035945 22.431809605781087 76-77 21.165662116281624 27.62370736800632 28.831634277677097 22.37899623803496 78-79 20.914689745037844 27.551530372394296 28.9375262266169 22.596253655950957 80-81 20.71070121994507 28.32957605607362 28.495727115972873 22.463995608008442 82-83 21.016934286210986 27.94180887948113 28.291139859541936 22.750116974765948 84-85 20.731520706532596 27.57911561640343 28.34974019618824 23.339623480875733 86-87 20.801361007919102 27.390221198532227 28.581387408086638 23.22703038546203 88-89 20.99732243932716 27.019093342660916 28.749638413664908 23.23394580434702 90-91 20.53512002344239 27.310825473005618 28.88726270521989 23.266791798332108 92-93 20.52627726448395 27.471203732566696 28.638766647694215 23.363752355255137 94-95 20.214273003885776 27.216905589132445 28.588948190066095 23.979873216915685 96-97 20.256803264102818 27.04889844101987 28.42337044109054 24.27092785378677 98-99 20.166577166724196 27.006349973591732 28.39538697734821 24.431685882335866 100-101 20.32966859610675 26.517927512536733 29.11449995362024 24.037903937736278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1002.0 1 653.5 2 371.5 3 439.0 4 366.5 5 360.0 6 729.5 7 1557.5 8 2537.5 9 3076.0 10 2757.0 11 2269.5 12 2280.5 13 2511.0 14 2706.5 15 2792.0 16 2884.5 17 3090.0 18 3263.5 19 3364.0 20 3679.0 21 4176.0 22 4664.5 23 5385.5 24 6342.5 25 7790.0 26 9930.0 27 12035.5 28 13893.5 29 16229.5 30 19375.5 31 23344.5 32 27812.0 33 32589.0 34 37721.5 35 41966.0 36 47743.5 37 53535.5 38 55020.5 39 54596.0 40 53577.5 41 53125.0 42 53009.5 43 53935.5 44 56450.5 45 58707.0 46 57922.5 47 58397.0 48 74765.0 49 74165.5 50 56579.5 51 49679.5 52 43900.5 53 41154.0 54 39149.0 55 36046.5 56 32102.5 57 28758.5 58 26738.0 59 24138.5 60 20720.5 61 17473.5 62 14703.5 63 11214.5 64 7761.5 65 5878.5 66 4279.0 67 3378.5 68 2562.5 69 1627.0 70 1186.0 71 992.0 72 930.0 73 941.5 74 814.5 75 654.5 76 425.0 77 217.0 78 150.0 79 88.5 80 44.0 81 32.5 82 18.5 83 9.0 84 6.0 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3510955012576084 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001167373611298662 16-17 0.001798386374162804 18-19 3.155063814320709E-5 20-21 0.0 22-23 0.0 24-25 5.048102102913134E-4 26-27 0.0011989242494418692 28-29 0.0 30-31 0.003470570195752779 32-33 0.0 34-35 0.011642185474843414 36-37 0.0302886126174788 38-39 0.023505225416689278 40-41 0.015238958223169022 42-43 0.011137375264552102 44-45 0.003565222110182401 46-47 0.001482879992730733 48-49 8.203165917233842E-4 50-51 6.310127628641418E-5 52-53 6.310127628641418E-5 54-55 0.0 56-57 0.0 58-59 0.005142754017342755 60-61 0.010916520797549652 62-63 0.004953450188483512 64-65 0.0022085446700244957 66-67 0.004637943807051441 68-69 0.004070032320473714 70-71 6.310127628641418E-5 72-73 0.0 74-75 0.0 76-77 0.22290525848175807 78-79 5.679114865777275E-4 80-81 0.00324971572875033 82-83 6.625634010073488E-4 84-85 3.155063814320709E-5 86-87 0.11418175944026643 88-89 0.4188978226273604 90-91 0.40488933929177645 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001451329354587526 100-101 6.941140391505559E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1584754.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.723384518547604 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0686298053723 33.409450568344454 2 14.084605071985425 12.879916269965511 3 5.165580062043432 7.085634103144645 4 2.4185532458572636 4.423377601556522 5 1.3374991767405804 3.057749457567521 6 0.8153219624019051 2.2367567755991478 7 0.5466214341197375 1.7495367412835687 8 0.3792541984000762 1.387262843497618 9 0.2824237302283338 1.1622031932953396 >10 1.6280765699822015 14.30493927312511 >50 0.16826232198887 5.411156536918224 >100 0.09826236162825612 7.9644402200593785 >500 0.004284236735992774 1.2690499763446792 >1k 0.0020730177754803747 1.7536560744668397 >5k 4.146035550960749E-4 1.2460487219808163 >10k+ 1.3820118503202498E-4 0.6588216428506511 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10426 0.6578939065621541 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8948 0.564630220210834 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5534 0.349202462969016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5237 0.330461383911951 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4413 0.2784659322519457 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3405 0.21485984575524025 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2146 0.1354153389106448 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2024 0.12771698320370228 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1931 0.12184856450906575 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1928 0.12165926068020652 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1725 0.10884970159406443 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1630 0.1028550803468551 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.310127628641418E-5 0.0 10-11 0.0 0.0 0.0 6.310127628641418E-5 0.0 12-13 0.0 0.0 0.0 6.310127628641418E-5 0.0 14-15 0.0 0.0 0.0 1.2620255257282835E-4 0.0 16-17 0.0 0.0 0.0 1.2620255257282835E-4 0.0 18-19 0.0 0.0 0.0 1.2620255257282835E-4 0.0 20-21 0.0 0.0 0.0 3.155063814320709E-4 0.0 22-23 0.0 0.0 0.0 8.834178680097984E-4 0.0 24-25 0.0 0.0 0.0 0.0019245889267356323 0.0 26-27 0.0 0.0 0.0 0.003596772748325608 0.0 28-29 0.0 0.0 0.0 0.008707976127525156 0.0 30-31 0.0 0.0 0.0 0.01949829437250198 0.0 32-33 0.0 0.0 0.0 0.033916936003947615 0.0 34-35 0.0 0.0 0.0 0.05505586355989636 0.0 36-37 0.0 0.0 0.0 0.09402090166675711 0.0 38-39 0.0 0.0 0.0 0.15254733542240623 0.0 40-41 0.0 0.0 0.0 0.2245143410270616 0.0 42-43 0.0 0.0 0.0 0.2975856189667292 0.0 44-45 0.0 0.0 0.0 0.3864006653398572 0.0 46-47 0.0 0.0 0.0 0.497900620537951 0.0 48-49 0.0 0.0 0.0 0.6271951356488137 0.0 50-51 0.0 0.0 0.0 0.7711606974962675 0.0 52-53 0.0 0.0 0.0 0.9130123665881267 0.0 54-55 0.0 0.0 0.0 1.0678944492331301 0.0 56-57 0.0 0.0 0.0 1.252812739390467 0.0 58-59 0.0 0.0 0.0 1.4785575553051138 0.0 60-61 0.0 0.0 0.0 1.7431096561359052 0.0 62-63 0.0 0.0 0.0 2.0044751425142326 0.0 64-65 0.0 0.0 0.0 2.266534742931711 0.0 66-67 0.0 0.0 0.0 2.5634262478592893 0.0 68-69 0.0 0.0 0.0 2.9022801015173334 0.0 70-71 0.0 0.0 0.0 3.300512256160893 0.0 72-73 0.0 0.0 0.0 3.7124058371204613 0.0 74-75 0.0 0.0 0.0 4.130262488689096 0.0 76-77 0.0 0.0 0.0 4.565314238045779 0.0 78-79 0.0 0.0 0.0 5.062899352202297 0.0 80-81 0.0 0.0 0.0 5.613331785248688 0.0 82-83 0.0 0.0 0.0 6.19771270493717 0.0 84-85 0.0 0.0 0.0 6.80332720409603 0.0 86-87 0.0 0.0 0.0 7.432793985691155 0.0 88-89 0.0 0.0 0.0 8.094316215639777 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCGT 40 4.5230973E-4 29.656382 92-93 CTAACGC 65 0.0058747577 29.200134 3 GAGTACT 8890 0.0 23.778482 12-13 GTATCAA 21195 0.0 22.402285 1 GTACTTT 9430 0.0 22.265873 14-15 ACCGTGC 150 3.0588148E-5 22.14343 8 AGTACTT 9320 0.0 21.077667 12-13 GGTATCA 16155 0.0 20.96009 1 GTACATG 13515 0.0 20.755337 1 TCTATAC 400 0.0 20.16634 3 TACATGG 13840 0.0 20.125198 2 ACTTTTT 10655 0.0 19.772778 16-17 CGGTATA 145 6.191011E-4 19.634571 2 TACCTGG 1120 0.0 19.064817 2 ATCAACG 24590 0.0 19.026398 3 AACGCTA 125 0.006068774 18.980087 5 ACATGGG 13660 0.0 18.96619 3 TATCAAC 24890 0.0 18.873325 2 CAACGCA 24890 0.0 18.835197 5 TCAACGC 24875 0.0 18.827482 4 >>END_MODULE