FastQCFastQC Report
Fri 27 May 2016
SRR939112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560913
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98480.6309128055183089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80710.5170691768215141No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA52610.3370463312176912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47170.3021949333499048No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA39510.25312109002872035No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT37250.23864238429688267No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20220.12953957075122058No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG19270.12345338913827997No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18990.12165956718920273No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA17880.11454834446250367No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG16950.10859029298878285No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16340.10468232374257885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT79650.023.82951412-13
GTATCAA198550.022.8876041
GTACTTT85150.022.34698314-15
GTACATG138950.022.1449531
GGTATCA150000.022.064791
AGTACTT83400.021.59460312-13
AGTATCG3101.0913936E-1121.3878295
TACATGG143950.021.055652
GTATAGG5200.021.003071
ACATGGG138900.020.7989713
ATGGGAG31800.020.1051375
ATGGGAT33450.019.9628875
CATGGGA91700.019.8324414
ACTTTTT97400.019.78034216-17
TATCAAC231350.019.5289652
ATCAACG230800.019.473533
TCAACGC232700.019.334884
CAACGCA234750.019.165425
TACCTGG11250.018.9435042
AACGCAG238450.018.856546
Produced by FastQC (version 0.11.5)