##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939112_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1560913 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27861386252789 33.0 31.0 34.0 31.0 34.0 2 32.37299516372789 34.0 31.0 34.0 31.0 34.0 3 32.41120036798976 34.0 31.0 34.0 30.0 34.0 4 35.85576069902679 37.0 35.0 37.0 35.0 37.0 5 35.90951129242949 37.0 35.0 37.0 35.0 37.0 6 36.0084418542225 37.0 37.0 37.0 35.0 37.0 7 35.95675671866401 37.0 37.0 37.0 35.0 37.0 8 35.96046672684512 37.0 37.0 37.0 35.0 37.0 9 37.650756320179276 39.0 38.0 39.0 35.0 39.0 10-11 37.575092590041855 39.0 38.0 39.0 35.0 39.0 12-13 37.495119522997115 39.0 38.0 39.0 35.0 39.0 14-15 38.769536482814864 40.0 38.0 41.0 35.0 41.0 16-17 38.70971059886105 40.0 38.0 41.0 35.0 41.0 18-19 38.69127939865963 40.0 38.5 41.0 35.0 41.0 20-21 38.7048823348899 40.0 38.5 41.0 34.5 41.0 22-23 38.729550269617846 40.5 39.0 41.0 35.0 41.0 24-25 38.68403492058815 41.0 39.0 41.0 35.0 41.0 26-27 38.56568815814847 40.0 38.0 41.0 34.5 41.0 28-29 38.436719727492815 40.0 38.0 41.0 34.0 41.0 30-31 38.21344399079257 40.0 38.0 41.0 34.0 41.0 32-33 37.989813333606676 40.0 38.0 41.0 34.0 41.0 34-35 37.69594942190884 40.0 37.5 41.0 33.0 41.0 36-37 37.43244082149357 40.0 37.0 41.0 33.0 41.0 38-39 37.23867089325286 40.0 37.0 41.0 32.5 41.0 40-41 37.02387769209431 40.0 36.5 41.0 31.5 41.0 42-43 36.77841237788397 40.0 36.0 41.0 31.0 41.0 44-45 36.439565177559544 40.0 36.0 41.0 30.0 41.0 46-47 36.153708118261555 40.0 36.0 41.0 29.5 41.0 48-49 35.94886422241342 40.0 36.0 41.0 28.0 41.0 50-51 34.815297521386526 38.5 34.5 40.0 25.5 40.5 52-53 34.92404189086771 38.5 35.0 40.0 26.0 40.5 54-55 35.443457771189046 39.0 35.0 41.0 26.0 41.0 56-57 35.38216319551442 39.0 35.0 41.0 26.0 41.0 58-59 35.20747889216119 39.0 35.0 41.0 26.0 41.0 60-61 34.93003549845507 39.0 35.0 41.0 25.5 41.0 62-63 34.57681658106505 38.5 35.0 40.5 24.5 41.0 64-65 34.214530214047805 38.0 34.5 40.0 23.0 41.0 66-67 33.84770003196847 37.0 34.0 40.0 22.0 41.0 68-69 33.45928632793756 37.0 34.0 39.5 21.0 41.0 70-71 33.03799058627867 36.0 34.0 39.0 19.5 41.0 72-73 32.596983944652905 35.5 33.0 39.0 18.0 40.5 74-75 32.15956718920273 35.0 33.0 37.5 14.5 40.0 76-77 31.71873032001143 35.0 32.5 37.0 10.0 39.0 78-79 31.292203665418892 35.0 32.0 37.0 7.0 39.0 80-81 30.856454523730662 35.0 32.0 36.0 2.0 38.5 82-83 30.476687041494305 35.0 31.0 36.0 2.0 37.0 84-85 30.092838934649144 35.0 31.0 35.0 2.0 37.0 86-87 29.68813508504318 34.5 31.0 35.0 2.0 36.5 88-89 29.267270180977413 34.0 30.0 35.0 2.0 36.0 90-91 28.858735240208777 34.0 29.5 35.0 2.0 36.0 92-93 28.47827617554598 34.0 29.0 35.0 2.0 35.0 94-95 28.042906299069838 34.0 29.0 35.0 2.0 35.0 96-97 27.482660148259384 34.0 28.0 35.0 2.0 35.0 98-99 26.837594728213553 34.0 26.5 35.0 2.0 35.0 100-101 24.099263059504274 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20261903922052937 1101 2 0.2554415421711056 1101 3 0.11571864607658 1101 4 0.22916537912244195 1101 5 0.16069741055556364 1101 6 0.09781069726531655 1101 7 0.22947255657423682 1101 8 0.15728041366740797 1101 9 0.24856411136216394 1101 10-11 0.13047037139718043 1101 12-13 0.1303860157839054 1101 14-15 0.28443275109459876 1101 16-17 0.2344565075902736 1101 18-19 0.44702234481430736 1101 20-21 0.37110663851768777 1101 22-23 0.311936524017959 1101 24-25 0.2946570490250977 1101 26-27 0.315871685434864 1101 28-29 0.16822008400856703 1101 30-31 0.25859955425811876 1101 32-33 0.15353871382134088 1101 34-35 0.2821945710289171 1101 36-37 0.07764164681073993 1101 38-39 -0.03266791182373652 1101 40-41 0.058001822538621184 1101 42-43 -0.007153396499795406 1101 44-45 -0.08187598777913507 1101 46-47 -0.05767961626160201 1101 48-49 -0.24243220878735627 1101 50-51 -0.05356425179606106 1101 52-53 0.023186615573258962 1101 54-55 -0.04177929324387719 1101 56-57 -0.0018221266230966648 1101 58-59 0.025749222179918263 1101 60-61 -0.13853753477298625 1101 62-63 -0.22725305511080052 1101 64-65 -0.2527145576492771 1101 66-67 -0.22814397773952777 1101 68-69 -0.19191506973562156 1101 70-71 -0.2419032534940655 1101 72-73 -0.18620238021302526 1101 74-75 -0.3980648925100283 1101 76-77 -0.394939994811498 1101 78-79 -0.4847644726012952 1101 80-81 -0.557204024249728 1101 82-83 -0.5871721157243712 1101 84-85 -0.5035133388206567 1101 86-87 -0.591149632231442 1101 88-89 -0.4039915816934396 1101 90-91 -0.6019780724177686 1101 92-93 -0.7686959201241663 1101 94-95 -0.9900343134161922 1101 96-97 -1.013987421606899 1101 98-99 -1.4119958453679402 1101 100-101 -1.6058166460236016 1102 1 0.13765876664432142 1102 2 0.11855298841690143 1102 3 0.19935569521570073 1102 4 0.1341302598005285 1102 5 0.15043740189791777 1102 6 0.23834034970272455 1102 7 0.2403644337473807 1102 8 0.2451345742248492 1102 9 0.1855867189892706 1102 10-11 0.1881554285795346 1102 12-13 0.2424047833261156 1102 14-15 0.2019948557404021 1102 16-17 0.2596788834158872 1102 18-19 0.2617190479268672 1102 20-21 0.32579538218155335 1102 22-23 0.302774430543316 1102 24-25 0.36470717958798815 1102 26-27 0.2792163295637238 1102 28-29 0.24801530275377104 1102 30-31 0.4085356692093143 1102 32-33 0.40938437033622677 1102 34-35 0.3611152977126224 1102 36-37 0.44754829783778405 1102 38-39 0.3584586992664285 1102 40-41 0.34765942660492044 1102 42-43 0.2260776509357214 1102 44-45 0.3504407703513621 1102 46-47 0.4105454266775297 1102 48-49 0.43578613145812284 1102 50-51 0.25073105771478055 1102 52-53 0.1907173013426089 1102 54-55 0.2658115073090954 1102 56-57 0.3014258870057134 1102 58-59 0.08399981913856891 1102 60-61 0.1290814729490748 1102 62-63 0.06813500292335561 1102 64-65 0.3154102939838097 1102 66-67 0.23025742982613195 1102 68-69 0.09596038583809019 1102 70-71 0.19480691086157265 1102 72-73 0.3094740332873158 1102 74-75 0.1870523348090316 1102 76-77 0.018823916768802462 1102 78-79 -0.11391525528042123 1102 80-81 0.0074054042059970016 1102 82-83 -0.3516509812934707 1102 84-85 -0.3007498740866019 1102 86-87 -0.15936350401450028 1102 88-89 -0.20301724742452265 1102 90-91 -0.39646545599337557 1102 92-93 -0.3998330739928804 1102 94-95 -0.5424148169560539 1102 96-97 -0.6313875444896198 1102 98-99 -0.707340266073679 1102 100-101 -0.7587113718415424 1103 1 0.1995239370244022 1103 2 0.18494387963577452 1103 3 0.1678121718430461 1103 4 0.16110710558471908 1103 5 0.11017691696999776 1103 6 0.1662155135297283 1103 7 0.1534064280680454 1103 8 0.20671031690724107 1103 9 0.18671302960526504 1103 10-11 0.2132380246913712 1103 12-13 0.26907445348775383 1103 14-15 0.2726350097377832 1103 16-17 0.28731786331978526 1103 18-19 0.3725309472278653 1103 20-21 0.3787968293200379 1103 22-23 0.4346449253008515 1103 24-25 0.3291601339348702 1103 26-27 0.4250428367721142 1103 28-29 0.19808561184070328 1103 30-31 0.3775860187069142 1103 32-33 0.3345427364349547 1103 34-35 0.3230118682674501 1103 36-37 0.3141077943428101 1103 38-39 0.06668622766310506 1103 40-41 0.12767389798980133 1103 42-43 0.11760028636603437 1103 44-45 0.10127014453222927 1103 46-47 0.11144852300780173 1103 48-49 0.01236842722047271 1103 50-51 -0.008617320397931394 1103 52-53 -0.010407644048392228 1103 54-55 -0.06495178998230244 1103 56-57 -0.029903978656150798 1103 58-59 0.026895870907907238 1103 60-61 0.007497948484243011 1103 62-63 -0.12073363512819668 1103 64-65 -0.12848578509002095 1103 66-67 -0.171183565149839 1103 68-69 -0.237454860994589 1103 70-71 -0.15119229730793649 1103 72-73 -0.05281230900189371 1103 74-75 -0.33978116235130074 1103 76-77 -0.20261557645968153 1103 78-79 -0.2763224199811596 1103 80-81 -0.2201844275982836 1103 82-83 -0.2544401359461581 1103 84-85 -0.3636867460831077 1103 86-87 -0.3399452263823477 1103 88-89 -0.35962561898415757 1103 90-91 -0.2511986910305133 1103 92-93 -0.2637576862689812 1103 94-95 -0.720266089345742 1103 96-97 -0.968751704603207 1103 98-99 -0.8974478458028159 1103 100-101 -0.9198557034273875 1104 1 0.1969286859610122 1104 2 0.23040697228023532 1104 3 0.30840475146749213 1104 4 0.2863275641385101 1104 5 0.3145906268984149 1104 6 0.2375824862447402 1104 7 0.2860527736433127 1104 8 0.2679637534670505 1104 9 0.28990870602397933 1104 10-11 0.40879529852683305 1104 12-13 0.3030688382787119 1104 14-15 0.3726332266171255 1104 16-17 0.3841267257536458 1104 18-19 0.4736784664207292 1104 20-21 0.42506799982527355 1104 22-23 0.4913716669234205 1104 24-25 0.4999449988067397 1104 26-27 0.4358242326637978 1104 28-29 0.3874230366254281 1104 30-31 0.4552425442801038 1104 32-33 0.4378275688864477 1104 34-35 0.504854512732642 1104 36-37 0.4200178667050878 1104 38-39 0.1748608632580897 1104 40-41 0.2884611295490984 1104 42-43 0.4218987418593443 1104 44-45 0.5881034195088901 1104 46-47 0.748527544431191 1104 48-49 0.7983080345448812 1104 50-51 0.8776690228430724 1104 52-53 0.880218976970383 1104 54-55 0.7630848644943029 1104 56-57 0.9416722675830869 1104 58-59 0.6243231520055303 1104 60-61 0.5738956881356643 1104 62-63 0.5026964081854288 1104 64-65 0.6985369221410238 1104 66-67 0.6709591947318927 1104 68-69 0.47589066239995503 1104 70-71 0.434728992504283 1104 72-73 0.44570040626939544 1104 74-75 0.3907876855582373 1104 76-77 0.4110632283955944 1104 78-79 0.4133886496318695 1104 80-81 0.5157110880153688 1104 82-83 0.5069475209684562 1104 84-85 0.4648937601934797 1104 86-87 0.3916748522046447 1104 88-89 0.3469659296723471 1104 90-91 0.44776029071033463 1104 92-93 0.2538441461306462 1104 94-95 0.17837079596393934 1104 96-97 0.20723500952689022 1104 98-99 0.17715468039284588 1104 100-101 0.06256110588893193 1105 1 0.009791794167256285 1105 2 0.14841030553197498 1105 3 0.1976827577178497 1105 4 0.21812288942633984 1105 5 0.18764147020702637 1105 6 0.1835543310792147 1105 7 0.1790133702831298 1105 8 0.26560712001050746 1105 9 0.2695363977165499 1105 10-11 0.3080539356062957 1105 12-13 0.3005334556279351 1105 14-15 0.3186280207062211 1105 16-17 0.2482679034481876 1105 18-19 0.294920092492724 1105 20-21 0.325856587211959 1105 22-23 0.31547608501192315 1105 24-25 0.27056847395626704 1105 26-27 0.295971475081366 1105 28-29 0.18683711665610758 1105 30-31 0.2981819256089935 1105 32-33 0.3150470167987862 1105 34-35 0.4186512476146973 1105 36-37 0.39421831467453927 1105 38-39 0.22666064580114664 1105 40-41 0.3504952596805495 1105 42-43 0.35177882435426966 1105 44-45 0.2873029495081525 1105 46-47 0.27644735259581665 1105 48-49 0.40643778249066287 1105 50-51 0.2104796231547681 1105 52-53 0.38597745493502345 1105 54-55 0.17812187909207466 1105 56-57 0.15649667008649715 1105 58-59 0.21394024624230923 1105 60-61 0.0675693436153324 1105 62-63 0.08777107996008482 1105 64-65 0.06683089495277983 1105 66-67 0.023635689799334614 1105 68-69 0.06446127062660167 1105 70-71 -0.01939136766641525 1105 72-73 0.021905956113144498 1105 74-75 0.07166045081081762 1105 76-77 0.03701791738729554 1105 78-79 -0.028629803608769322 1105 80-81 0.04508944880620902 1105 82-83 -0.08363522690389047 1105 84-85 -0.11664621638808015 1105 86-87 -0.05233738127800791 1105 88-89 -0.025151133965430006 1105 90-91 -0.08280148664313813 1105 92-93 0.051179278685538065 1105 94-95 -0.21715195745703042 1105 96-97 -0.26813891032819015 1105 98-99 -0.47752074574931314 1105 100-101 -0.6442654813835134 1106 1 0.07840826556628855 1106 2 0.15882785948546285 1106 3 0.1739055680230237 1106 4 0.2189325789856511 1106 5 0.11986953454080407 1106 6 0.14497913303679866 1106 7 0.20641020625058104 1106 8 0.15393626535001914 1106 9 0.1765789061764309 1106 10-11 0.2175446297420791 1106 12-13 0.1408598190812711 1106 14-15 0.13879079105444703 1106 16-17 0.19945204418026918 1106 18-19 0.2105313718119035 1106 20-21 0.2934126217948432 1106 22-23 0.3775591408664738 1106 24-25 0.28544464420692606 1106 26-27 0.2736011996417247 1106 28-29 0.19067769734823514 1106 30-31 0.20473416776667364 1106 32-33 0.15169880285876047 1106 34-35 -0.02291438128130352 1106 36-37 0.14365448422827143 1106 38-39 0.17037318616463892 1106 40-41 0.45089361920013715 1106 42-43 0.37659680149517527 1106 44-45 0.23245507241447427 1106 46-47 0.3345148709287784 1106 48-49 0.4321010213263392 1106 50-51 0.42329052845835946 1106 52-53 0.2764798883594679 1106 54-55 0.2858635857027707 1106 56-57 0.29730134566861466 1106 58-59 0.40264171326621323 1106 60-61 0.4760666798189561 1106 62-63 0.24203726794358005 1106 64-65 0.17805721891378568 1106 66-67 0.2674684010639439 1106 68-69 0.28974928393009947 1106 70-71 0.10949640055232379 1106 72-73 0.14532408502849847 1106 74-75 0.1830094623410332 1106 76-77 0.26542780905441177 1106 78-79 0.21731497294982205 1106 80-81 0.24439818783934442 1106 82-83 0.36907125603309865 1106 84-85 0.47007937580327663 1106 86-87 0.475201501243923 1106 88-89 0.31398489388053363 1106 90-91 0.4201860887601079 1106 92-93 0.21019833954244405 1106 94-95 0.45544888349729007 1106 96-97 0.6085869432094704 1106 98-99 0.6163224193673429 1106 100-101 0.33986716831951824 1107 1 0.12062965034093054 1107 2 0.18059266493008153 1107 3 0.0861436071987356 1107 4 0.18540121943107124 1107 5 0.16716672442622382 1107 6 0.134666683007012 1107 7 0.2767981948833196 1107 8 0.2011385410462836 1107 9 0.15696626828200522 1107 10-11 0.21444495675184783 1107 12-13 0.20317121804161076 1107 14-15 0.2658441870654329 1107 16-17 0.20782389646574018 1107 18-19 0.2550587505812345 1107 20-21 0.22928616430097293 1107 22-23 0.17505761429096367 1107 24-25 0.2510013135885458 1107 26-27 0.3039124901287025 1107 28-29 0.3434187069172694 1107 30-31 0.4335622253361038 1107 32-33 0.44047008023542844 1107 34-35 0.30825408252085396 1107 36-37 0.3941201715799991 1107 38-39 0.3380945068838912 1107 40-41 0.23557493662778484 1107 42-43 0.32452982293643373 1107 44-45 0.46671614908738235 1107 46-47 0.5494962107056764 1107 48-49 0.5941197759960701 1107 50-51 0.4245087497384006 1107 52-53 0.4780914190775363 1107 54-55 0.5233945263257667 1107 56-57 0.36546142242287516 1107 58-59 0.5158345985360029 1107 60-61 0.3817806542734772 1107 62-63 0.374758163185426 1107 64-65 0.37254901655112604 1107 66-67 0.39631932219913324 1107 68-69 0.47197283817191504 1107 70-71 0.5280308039067734 1107 72-73 0.4672169077390791 1107 74-75 0.48362514216507435 1107 76-77 0.5780912788740373 1107 78-79 0.4716665313660009 1107 80-81 0.4953218478426926 1107 82-83 0.4943675706507271 1107 84-85 0.5354579525018899 1107 86-87 0.557629097614555 1107 88-89 0.6137708927752321 1107 90-91 0.5983068252194066 1107 92-93 0.3634143876148457 1107 94-95 0.5886847934157764 1107 96-97 0.5216862234513258 1107 98-99 0.41383865997995883 1107 100-101 0.7087252150322634 1108 1 -0.06327158611443195 1108 2 -0.05610658984779349 1108 3 -0.02527342507177366 1108 4 0.11818289364641998 1108 5 0.12105468356959648 1108 6 0.19708824994823715 1108 7 -0.08560053034912585 1108 8 0.08622700034117514 1108 9 0.0789099611947961 1108 10-11 0.10509437110317776 1108 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sequence quality scores pass #Quality Count 2 4589.0 3 980.0 4 892.0 5 1083.0 6 1158.0 7 1393.0 8 1719.0 9 2249.0 10 2643.0 11 3816.0 12 5542.0 13 9900.0 14 8561.0 15 9753.0 16 13315.0 17 20659.0 18 16085.0 19 8938.0 20 8078.0 21 8050.0 22 8229.0 23 9211.0 24 9797.0 25 11226.0 26 12849.0 27 15428.0 28 17995.0 29 21689.0 30 26496.0 31 32848.0 32 41510.0 33 55012.0 34 77887.0 35 118014.0 36 190432.0 37 328879.0 38 379012.0 39 74979.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65101776301189 20.28919971568889 15.43545591087297 20.62432661042624 2 15.045403992267403 21.786540304301035 40.4002529641835 22.76780273924806 3 18.691753030926936 27.878306247089657 31.034113401757686 22.39582732022572 4 12.388607922981985 18.29787261339348 38.82775635252369 30.48576311110085 5 12.97416162726773 38.89644059239853 33.211408425135176 14.917989355198563 6 27.991878477195243 37.582558343794034 19.26290833603666 15.162654842974064 7 26.761653363528005 35.1773498916749 21.2444146373209 16.816582107476194 8 26.440330009642803 33.43258242547342 20.48160173160173 19.64548583328204 9 27.163394804044078 17.44340144629172 21.469911451658096 33.92329229800611 10-11 23.986602908238318 27.692761909585663 27.330362114491408 20.990273067684615 12-13 26.69819032811323 25.336656675268888 27.736932373731705 20.228220622886177 14-15 23.751246173326283 25.99163340866471 27.65850843871712 22.598611979291885 16-17 20.9010710015355 29.675718301912802 27.984907886174433 21.438302810377273 18-19 20.785333348294508 27.83259088511903 31.325522532328154 20.05655323425831 20-21 22.632561306678944 26.420315743983462 30.684853598765855 20.262269350571742 22-23 21.911037838447886 26.671719658961496 30.435814173002147 20.981428329588475 24-25 21.456718730509728 26.880180904993132 31.027630531309015 20.63546983318813 26-27 21.46593165069789 26.723170671875184 31.035141788582326 20.775755888844603 28-29 20.814242853034003 26.833094932794133 31.542395375510505 20.810266838661352 30-31 21.154785191474026 27.120824806259215 31.08086054863326 20.6435294536335 32-33 20.863132900485503 26.993109723430308 30.869246974943838 21.274510401140354 34-35 21.3427150668111 26.870759013953666 30.76200333653701 21.024522582698225 36-37 21.788305439910022 26.494865681909975 30.773152743789122 20.94367613439088 38-39 21.538152399641785 26.643451200022156 30.83247541943858 20.985920980897475 40-41 21.167377304341247 26.84529989231787 31.079927825609676 20.907394977731204 42-43 21.13765478652389 27.0839626122961 30.412190869728466 21.36619173145154 44-45 21.190448892518802 27.323591397794054 29.96624224170581 21.519717467981337 46-47 21.417309141499043 27.234169999806774 29.286869038187803 22.061651820506377 48-49 21.60153947728627 27.38073076861141 29.123335319409332 21.89439443469299 50-51 21.953103965351325 27.574721513328843 28.80766996157161 21.664504559748224 52-53 21.689973375570002 27.727313277143022 28.88523604987113 21.697477297415848 54-55 21.178111448388506 27.5858881935092 29.082221187418245 22.153779170684047 56-57 21.641364354581782 28.098926910679765 28.293024339927086 21.966684394811363 58-59 21.687137152523494 27.371950149766377 28.746388626446763 22.194524071263366 60-61 21.786518730098877 27.484710500907443 29.00605145327859 21.722719315715086 62-63 22.143106223478263 27.296328984028435 28.96780024644952 21.59276454604378 64-65 21.7340766044175 27.333731851686753 29.164361122043886 21.767830421851862 66-67 21.512548361109598 27.611805924866058 28.81988746046476 22.05575825355958 68-69 21.43692238005753 27.781362747337397 28.97076007189412 21.810954800710952 70-71 21.51565573017926 27.78471297156857 28.626944382251416 22.07268691600075 72-73 21.45526203986764 27.69837110657236 28.41043824000531 22.43592861355469 74-75 21.35862566139015 28.020104070098174 28.399720916388947 22.22154935212273 76-77 21.466005510635476 27.585348510981984 28.736811122260626 22.21183485612192 78-79 21.208184715969804 27.56081440222579 28.898684569324345 22.33231631248006 80-81 21.111392655302655 28.43876805563113 28.41668096746598 22.033158321600236 82-83 21.431138349298845 27.998959247051307 28.241307845190576 22.328594558459272 84-85 21.240300692225805 27.642117519321335 28.286237206316006 22.831344582136854 86-87 21.34954833233621 27.567569830261572 28.536013307564843 22.546868529837372 88-89 21.702010084072583 27.22979763906337 28.54355450516264 22.524637771701414 90-91 21.241368603393486 27.545001794080758 28.754605183057436 22.45902441946832 92-93 21.426525996793664 27.63590927817518 28.52642907231777 22.411135652713384 94-95 21.215482782056363 27.516725128115933 28.405178106106728 22.862613983720983 96-97 21.421222838515998 27.329880370291633 28.141938684137628 23.10695810705474 98-99 21.443770240349213 27.248092003842082 28.15324267546575 23.154895080342953 100-101 21.90749818217885 26.8125560722955 28.733328052152306 22.546617693373342 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2082.0 1 1229.0 2 433.5 3 483.0 4 402.0 5 406.5 6 791.5 7 1675.0 8 2753.5 9 3262.0 10 2901.0 11 2542.5 12 2617.0 13 2758.5 14 3037.0 15 3249.0 16 3326.0 17 3443.5 18 3572.0 19 3683.0 20 3783.0 21 4068.5 22 4694.5 23 5424.5 24 6209.5 25 7652.0 26 9727.5 27 11772.5 28 13338.0 29 15618.0 30 19018.5 31 22604.5 32 26944.0 33 32003.0 34 37119.5 35 41306.0 36 46722.0 37 52344.0 38 53748.0 39 53117.5 40 52086.0 41 51843.5 42 51647.0 43 52416.5 44 54970.0 45 57056.0 46 56428.5 47 57013.0 48 73152.5 49 72506.5 50 55038.0 51 48453.0 52 43292.5 53 40775.5 54 38602.0 55 35762.5 56 32115.5 57 28513.5 58 26150.5 59 23665.0 60 20526.0 61 17765.5 62 15186.0 63 11338.0 64 7827.5 65 5937.0 66 4355.5 67 3488.5 68 2749.0 69 1889.0 70 1428.0 71 1159.5 72 986.0 73 939.0 74 807.0 75 695.0 76 494.5 77 278.0 78 200.0 79 150.5 80 114.5 81 83.5 82 58.0 83 38.0 84 25.0 85 18.0 86 12.5 87 11.0 88 11.5 89 8.5 90 6.5 91 4.0 92 2.5 93 2.5 94 3.5 95 2.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4022005070109609 2 0.1152530602282126 3 0.11653436162041063 4 0.12845046456785228 5 0.1300520913080998 6 0.07092003205816083 7 0.08046572743003613 8 0.07694214860149157 9 0.07687808353188166 10-11 0.07505222904799948 12-13 0.07931255617705792 14-15 0.07290604921606778 16-17 0.07418735060826581 18-19 0.08459792441987478 20-21 0.0829642651448223 22-23 0.0923177653078679 24-25 0.10516281176465311 26-27 0.10714882892256007 28-29 0.10010167126547091 30-31 0.09094036631125502 32-33 0.08946686971022728 34-35 0.3464638964503467 36-37 0.5455781327979202 38-39 0.5261343841713151 40-41 0.4952550206193427 42-43 0.5259742214972903 44-45 0.5369293484005835 46-47 0.5332135743632093 48-49 0.5405810573683478 50-51 0.5391075607673201 52-53 0.5376020316314875 54-55 0.5307150366484231 56-57 0.5014372998366982 58-59 0.49077046574664956 60-61 0.4371480024831621 62-63 0.5163324285210001 64-65 0.5442968314057222 66-67 0.5547074052173312 68-69 0.5707236726198064 70-71 0.5666875732343827 72-73 0.5510556962495669 74-75 0.5567254549100431 76-77 0.5303947113003735 78-79 0.5027506337637011 80-81 0.510566572256109 82-83 0.5247569851747023 84-85 0.5186708035617617 86-87 0.51969584467552 88-89 0.5295938979302498 90-91 0.5500626876706133 92-93 0.5556683812614797 94-95 0.5514721192020311 96-97 0.5524330952461796 98-99 0.552881550733449 100-101 0.5272555228894884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1560913.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.118976478195044 #Duplication Level Percentage of deduplicated Percentage of total 1 74.16345110777448 34.94505908288994 2 13.703339715325088 12.913746834382378 3 4.854285411619339 6.86186880385611 4 2.299002352922841 4.333065511627456 5 1.2416452616820737 2.925252693972998 6 0.7727164637923332 2.1845765329047 7 0.514494736085673 1.6969725757443197 8 0.36862562256239034 1.389540963102182 9 0.26916866186327504 1.1414656662302602 >10 1.5547077603558932 14.08019353192217 >50 0.16099587531954349 5.273898990320029 >100 0.09129884119394198 7.601558394659028 >500 0.003951684686751829 1.2572307701882952 >1k 0.001907709848776745 1.9072590791299378 >5k 4.0879496759501667E-4 1.4883105690702563 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9848 0.6309128055183089 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8071 0.5170691768215141 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5261 0.3370463312176912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4717 0.3021949333499048 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3951 0.25312109002872035 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3725 0.23864238429688267 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2022 0.12953957075122058 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1927 0.12345338913827997 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1899 0.12165956718920273 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1788 0.11454834446250367 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1695 0.10859029298878285 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1634 0.10468232374257885 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2813013921980277E-4 0.0 3 0.0 0.0 0.0 1.2813013921980277E-4 0.0 4 0.0 0.0 0.0 1.2813013921980277E-4 0.0 5 0.0 0.0 0.0 1.2813013921980277E-4 0.0 6 0.0 0.0 0.0 1.2813013921980277E-4 0.0 7 0.0 0.0 0.0 1.2813013921980277E-4 0.0 8 0.0 0.0 0.0 1.2813013921980277E-4 0.0 9 0.0 0.0 0.0 1.2813013921980277E-4 0.0 10-11 0.0 0.0 0.0 1.2813013921980277E-4 0.0 12-13 0.0 0.0 0.0 1.2813013921980277E-4 0.0 14-15 0.0 0.0 0.0 1.2813013921980277E-4 0.0 16-17 0.0 0.0 0.0 1.2813013921980277E-4 0.0 18-19 0.0 0.0 0.0 1.6016267402475348E-4 0.0 20-21 0.0 0.0 0.0 2.882928132445562E-4 0.0 22-23 0.0 0.0 0.0 5.445530916841617E-4 0.0 24-25 0.0 0.0 0.0 0.0016016267402475346 0.0 26-27 0.0 0.0 0.0 0.0033634161545198224 0.0 28-29 0.0 0.0 0.0 0.008552686792921834 0.0 30-31 0.0 0.0 0.0 0.020532854809973394 0.0 32-33 0.0 0.0 0.0 0.03526782082025071 0.0 34-35 0.0 0.0 0.0 0.059548482202403344 0.0 36-37 0.0 0.0 0.0 0.0993328904301521 0.0 38-39 0.0 0.0 0.0 0.15833681954087125 0.0 40-41 0.0 0.0 0.0 0.23041002285201034 0.0 42-43 0.0 0.0 0.0 0.30517395908676526 0.0 44-45 0.0 0.0 0.0 0.3968510736985341 0.0 46-47 0.0 0.0 0.0 0.5127127520880408 0.0 48-49 0.0 0.0 0.0 0.642540615652506 0.0 50-51 0.0 0.0 0.0 0.7857260462306355 0.0 52-53 0.0 0.0 0.0 0.9303209083401829 0.0 54-55 0.0 0.0 0.0 1.083052034290188 0.0 56-57 0.0 0.0 0.0 1.270090005016295 0.0 58-59 0.0 0.0 0.0 1.4963678308784667 0.0 60-61 0.0 0.0 0.0 1.7565681110990812 0.0 62-63 0.0 0.0 0.0 2.023078800676271 0.0 64-65 0.0 0.0 0.0 2.2837275363841547 0.0 66-67 0.0 0.0 0.0 2.576953359988673 0.0 68-69 0.0 0.0 0.0 2.933923927855044 0.0 70-71 0.0 0.0 0.0 3.3413777705740166 0.0 72-73 0.0 0.0 0.0 3.753764623652952 0.0 74-75 0.0 0.0 0.0 4.165574891105398 0.0 76-77 0.0 0.0 0.0 4.607015253252424 0.0 78-79 0.0 0.0 0.0 5.117037272416848 0.0 80-81 0.0 0.0 0.0 5.677574598968681 0.0 82-83 0.0 0.0 0.0 6.263641855760058 0.0 84-85 0.0 0.0 0.0 6.8629385494258806 0.0 86-87 0.0 0.0 0.0 7.482928260575701 0.0 88-89 0.0 0.0 0.0 8.141068720678218 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 7965 0.0 23.829514 12-13 GTATCAA 19855 0.0 22.887604 1 GTACTTT 8515 0.0 22.346983 14-15 GTACATG 13895 0.0 22.144953 1 GGTATCA 15000 0.0 22.06479 1 AGTACTT 8340 0.0 21.594603 12-13 AGTATCG 310 1.0913936E-11 21.387829 5 TACATGG 14395 0.0 21.05565 2 GTATAGG 520 0.0 21.00307 1 ACATGGG 13890 0.0 20.798971 3 ATGGGAG 3180 0.0 20.105137 5 ATGGGAT 3345 0.0 19.962887 5 CATGGGA 9170 0.0 19.832441 4 ACTTTTT 9740 0.0 19.780342 16-17 TATCAAC 23135 0.0 19.528965 2 ATCAACG 23080 0.0 19.47353 3 TCAACGC 23270 0.0 19.33488 4 CAACGCA 23475 0.0 19.16542 5 TACCTGG 1125 0.0 18.943504 2 AACGCAG 23845 0.0 18.85654 6 >>END_MODULE