FastQCFastQC Report
Fri 27 May 2016
SRR939112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560913
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101360.6493635455659604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85180.54570626293714No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA54210.3472967423552754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.3152001424807148No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA43510.2787471178726809No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33860.21692432569912606No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21050.1348569715288424No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20090.1287067248462919No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG19250.12332525899906017No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18940.12133924184115322No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA16770.10743712173580462No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG15990.10244004630623231No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG1401.7931778E-523.7273945
GAGTACT86900.023.62044712-13
AGTACTT90500.022.49730312-13
GTACTTT93300.021.89844314-15
GTATCAA210950.021.7321051
CATTCCG3301.8189894E-1221.571749
GTACATG131300.021.5226751
TACATGG134000.021.0005152
GGTATCA163300.020.1358131
ACATGGG134950.019.8329013
TTTACGC1200.004790568319.7728273
TTCGCCG600.00479162219.77282792-93
ACTTTTT104100.019.60219416-17
TACTTTT103050.019.45810114-15
ATGGGAT33100.018.7811995
GTACACG2051.5460495E-518.596981
ATCAACG246950.018.5245673
CATGGGA90850.018.4909134
TCAACGC247250.018.4828954
TATCAAC248550.018.4625952
Produced by FastQC (version 0.11.5)