##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939112_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1560913 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.501178476955474 34.0 31.0 34.0 31.0 34.0 2 32.711731531481895 34.0 31.0 34.0 31.0 34.0 3 32.8033702070519 34.0 31.0 34.0 31.0 34.0 4 36.25682469170287 37.0 35.0 37.0 35.0 37.0 5 36.23246843353858 37.0 37.0 37.0 35.0 37.0 6 36.28371152011675 37.0 37.0 37.0 35.0 37.0 7 36.24416479329726 37.0 37.0 37.0 35.0 37.0 8 36.227594363042655 37.0 37.0 37.0 35.0 37.0 9 37.95152132117549 39.0 38.0 39.0 35.0 39.0 10-11 37.8825030607087 39.0 38.0 39.0 35.0 39.0 12-13 37.80313380694504 39.0 38.0 39.0 35.0 39.0 14-15 39.16144205346487 40.5 38.5 41.0 36.0 41.0 16-17 39.26544817039771 41.0 39.0 41.0 36.0 41.0 18-19 39.358887715074445 41.0 39.0 41.0 36.0 41.0 20-21 39.35058872595718 41.0 39.0 41.0 36.0 41.0 22-23 39.27501212431443 41.0 39.0 41.0 36.0 41.0 24-25 39.1954817469007 41.0 39.0 41.0 36.0 41.0 26-27 39.0333128752211 40.5 39.0 41.0 35.0 41.0 28-29 38.88095620960297 40.0 38.0 41.0 35.0 41.0 30-31 38.65995478287387 40.0 38.0 41.0 35.0 41.0 32-33 38.50411554007174 40.0 38.0 41.0 35.0 41.0 34-35 38.38831440317301 40.0 38.0 41.0 34.0 41.0 36-37 38.21228729596076 40.0 38.0 41.0 34.0 41.0 38-39 38.0521832414747 40.0 38.0 41.0 34.0 41.0 40-41 37.82563602199482 40.0 37.0 41.0 33.0 41.0 42-43 37.5881906935236 40.0 37.0 41.0 33.0 41.0 44-45 37.24738406304515 40.0 37.0 41.0 32.5 41.0 46-47 36.79837953812929 40.0 37.0 41.0 31.0 41.0 48-49 36.35398257301976 40.0 36.0 41.0 30.0 41.0 50-51 36.07133645501062 40.0 36.0 41.0 29.5 41.0 52-53 35.78153747197954 39.0 35.0 41.0 28.5 41.0 54-55 35.54472542672141 39.0 35.0 41.0 28.0 41.0 56-57 35.33509426854668 39.0 35.0 40.0 27.5 41.0 58-59 35.02788976707863 38.5 35.0 40.0 26.5 41.0 60-61 34.66750965620761 38.0 34.5 40.0 26.0 41.0 62-63 34.27777396946531 38.0 34.0 40.0 25.5 41.0 64-65 33.83767545020126 37.0 34.0 40.0 23.5 41.0 66-67 33.42971773570981 36.5 33.0 39.0 22.5 41.0 68-69 32.96505218420245 36.0 33.0 39.0 21.5 40.0 70-71 32.4717117481884 35.0 32.0 38.5 20.0 40.0 72-73 31.920375446933946 35.0 31.5 37.5 18.0 39.5 74-75 31.38861743095227 35.0 31.0 37.0 15.0 39.0 76-77 29.369170158746833 33.0 28.5 35.0 9.0 37.5 78-79 30.344560843557588 34.0 30.5 36.0 8.0 38.0 80-81 30.364757677077456 34.0 31.0 36.0 7.0 37.0 82-83 29.990659312850877 34.0 30.0 35.0 2.0 37.0 84-85 29.530867191188747 34.0 30.0 35.0 2.0 36.5 86-87 28.840466444958814 34.0 29.0 35.0 2.0 36.0 88-89 28.176476844000916 33.5 28.5 35.0 2.0 35.5 90-91 27.614043511713977 33.0 26.5 35.0 2.0 35.0 92-93 27.196660544181512 33.0 26.0 35.0 2.0 35.0 94-95 26.703528319643695 33.0 25.5 34.0 2.0 35.0 96-97 25.944570261122816 32.0 24.5 34.0 2.0 35.0 98-99 24.78756791698192 32.0 11.0 34.0 2.0 35.0 100-101 18.939531543397997 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14705738584589056 1101 2 0.10549847747123664 1101 3 0.13360504081325786 1101 4 0.062961914849339 1101 5 0.15493020956787973 1101 6 0.08365400297621761 1101 7 0.12423180088590868 1101 8 0.12835125420651394 1101 9 0.16244696532990588 1101 10-11 0.19288299097437545 1101 12-13 0.19425869191491074 1101 14-15 0.29739402147318117 1101 16-17 0.22301109342964054 1101 18-19 0.21777919845457916 1101 20-21 0.14875665268701965 1101 22-23 0.27947316339701445 1101 24-25 0.1135550650738395 1101 26-27 0.1869909925253097 1101 28-29 0.27275789522575167 1101 30-31 0.28711804028552024 1101 32-33 0.32576663963916275 1101 34-35 0.3163488319133734 1101 36-37 0.2513453695603687 1101 38-39 0.1624898798179757 1101 40-41 0.24705996399988805 1101 42-43 0.32209949815896977 1101 44-45 0.39617871218757017 1101 46-47 0.5299094679245613 1101 48-49 0.5675143141366021 1101 50-51 0.4691502222842914 1101 52-53 0.5278524686084083 1101 54-55 0.44226449790956934 1101 56-57 0.45949520286492174 1101 58-59 0.3157799869239355 1101 60-61 0.41433103302350105 1101 62-63 0.3330070726941372 1101 64-65 0.31010275872102255 1101 66-67 0.40259217046459383 1101 68-69 0.4946003556013707 1101 70-71 0.4817388696094014 1101 72-73 0.32693094532713474 1101 74-75 0.237779050360583 1101 76-77 0.42497105707484906 1101 78-79 0.5471202325284636 1101 80-81 0.6639037989141663 1101 82-83 0.5349269929523714 1101 84-85 0.2232027402939174 1101 86-87 0.2947882777107651 1101 88-89 0.5655022040589692 1101 90-91 0.5339424859561852 1101 92-93 0.2696508751381721 1101 94-95 0.06290893623404514 1101 96-97 -0.008342799390561595 1101 98-99 -0.30446701847656854 1101 100-101 -0.6844797052496894 1102 1 0.1104194849061173 1102 2 0.12160588813689799 1102 3 0.1242526885867008 1102 4 0.09737605753293366 1102 5 0.12469128646732486 1102 6 0.0946191908610956 1102 7 0.10918914103365296 1102 8 0.22185033956810685 1102 9 0.15625046469455128 1102 10-11 0.1559256647905869 1102 12-13 0.2333996300127481 1102 14-15 0.28246481867481066 1102 16-17 0.1848999962824749 1102 18-19 0.15218880285600989 1102 20-21 0.19480799811313432 1102 22-23 0.16559719115733884 1102 24-25 0.19889047903499346 1102 26-27 0.21608138114190467 1102 28-29 0.2629796426738409 1102 30-31 0.2916667953998129 1102 32-33 0.18912420041723266 1102 34-35 0.2994960956783288 1102 36-37 0.24790849357415823 1102 38-39 0.23133212913024437 1102 40-41 0.22735509197944737 1102 42-43 0.20307083017969063 1102 44-45 0.14780725728412847 1102 46-47 0.12183411847683345 1102 48-49 0.011211687518539293 1102 50-51 -0.04453095292132758 1102 52-53 -0.0400751423002248 1102 54-55 -0.13939316201251728 1102 56-57 -0.11808149410350666 1102 58-59 -0.1426737854358393 1102 60-61 -0.19016291360630078 1102 62-63 -0.20259051433614417 1102 64-65 0.009041847992520502 1102 66-67 -0.069232079322461 1102 68-69 0.08530316095794177 1102 70-71 -0.05947047785544868 1102 72-73 0.06380658243212878 1102 74-75 -0.038076939473665306 1102 76-77 -0.07981333961290105 1102 78-79 0.0656870430237646 1102 80-81 0.2247708900847627 1102 82-83 0.07970587388181016 1102 84-85 -0.036006481495423515 1102 86-87 0.3091763776367564 1102 88-89 0.11134758129494671 1102 90-91 0.3855511324309866 1102 92-93 0.041375282717798 1102 94-95 0.026454312073628472 1102 96-97 0.21905306448890371 1102 98-99 0.3834283357189001 1102 100-101 -0.33431632709209325 1103 1 0.14528050500222633 1103 2 0.14021390473803308 1103 3 0.10247735001090774 1103 4 0.005619286016987246 1103 5 0.1101106835839758 1103 6 0.10767756788163041 1103 7 0.08656736296462242 1103 8 0.11087802633088728 1103 9 0.06908767526685011 1103 10-11 0.05240747999373241 1103 12-13 0.08750144849242503 1103 14-15 0.08321023151884788 1103 16-17 0.21203384294040006 1103 18-19 0.18203603417973113 1103 20-21 0.24907227836651913 1103 22-23 0.2781702306942506 1103 24-25 0.22509255963787922 1103 26-27 0.10309877410826118 1103 28-29 0.0517383801137683 1103 30-31 0.13849537775018206 1103 32-33 0.39469636621014814 1103 34-35 0.3645012835332935 1103 36-37 0.3800486202670399 1103 38-39 0.20547476783711005 1103 40-41 0.3671268263612717 1103 42-43 0.38902471287955365 1103 44-45 0.47270350018495577 1103 46-47 0.5569279094335826 1103 48-49 0.3556238216382397 1103 50-51 0.5111702266330624 1103 52-53 0.2784079562144086 1103 54-55 0.1376075752089818 1103 56-57 0.2224056181726013 1103 58-59 0.266886885162684 1103 60-61 0.36514235070126233 1103 62-63 0.26701616434449704 1103 64-65 0.28587534515285284 1103 66-67 0.17136493356526472 1103 68-69 0.09638878786925176 1103 70-71 0.21537661694577537 1103 72-73 0.1731611040572929 1103 74-75 0.07564971517902208 1103 76-77 0.0892492969095997 1103 78-79 0.08512102074329064 1103 80-81 0.1262767332477175 1103 82-83 0.0830267836677443 1103 84-85 -0.04039226677283736 1103 86-87 -0.01666869663148418 1103 88-89 0.27396294280652356 1103 90-91 0.3624242211160045 1103 92-93 0.334325260874202 1103 94-95 0.315448550824172 1103 96-97 -0.07729291082782908 1103 98-99 -0.22659173308844416 1103 100-101 -0.6570009055106141 1104 1 0.1225003281166579 1104 2 0.10751795598816472 1104 3 0.1658818784889604 1104 4 0.11296639152585897 1104 5 0.10504013684676039 1104 6 0.09048342170463997 1104 7 0.07963912870796008 1104 8 0.12463561269876777 1104 9 0.20791010238584562 1104 10-11 0.28008118836596907 1104 12-13 0.21745285034946704 1104 14-15 0.060856382571856216 1104 16-17 0.20334356127217035 1104 18-19 0.23053772652333748 1104 20-21 0.3314224638110659 1104 22-23 0.3213922648782983 1104 24-25 0.2952932093421978 1104 26-27 0.24997036996882827 1104 28-29 0.25590569518794837 1104 30-31 0.1651335793271116 1104 32-33 0.19805949386861954 1104 34-35 0.20683937617775427 1104 36-37 0.22734540935304892 1104 38-39 0.30647720584935456 1104 40-41 0.3326639671435032 1104 42-43 0.30829392519411414 1104 44-45 0.4866896502005247 1104 46-47 0.3574380440429863 1104 48-49 0.39395605267880285 1104 50-51 0.34180618472000646 1104 52-53 0.15524375998035822 1104 54-55 0.20659732388622132 1104 56-57 0.3423480395856018 1104 58-59 0.4046718602638322 1104 60-61 0.35613921240890534 1104 62-63 0.3331140155220069 1104 64-65 0.3716645219346759 1104 66-67 0.42930549404871243 1104 68-69 0.3882370086390381 1104 70-71 0.2968944092478836 1104 72-73 0.18186921693207125 1104 74-75 0.08353516102402736 1104 76-77 0.2535541392168916 1104 78-79 0.512082777668283 1104 80-81 0.5833310827653015 1104 82-83 0.44887532215721393 1104 84-85 0.27547539973821245 1104 86-87 0.29126165143366833 1104 88-89 0.534536037785255 1104 90-91 0.6554071550303107 1104 92-93 0.5831127388283441 1104 94-95 0.6952021883903114 1104 96-97 0.3318497971812988 1104 98-99 0.14502114817517509 1104 100-101 -0.8566974507953979 1105 1 0.027846382476809595 1105 2 0.04586197857430818 1105 3 0.10078861271823314 1105 4 0.09742974615609512 1105 5 0.05530196234266782 1105 6 0.08931045980244079 1105 7 0.017061611225727802 1105 8 0.012404577962776386 1105 9 0.21553129484951938 1105 10-11 0.13874208406006971 1105 12-13 0.12607070344159865 1105 14-15 0.09620180455255678 1105 16-17 0.17331828649310665 1105 18-19 0.15986090710643452 1105 20-21 0.22859424867149158 1105 22-23 0.1256195685754733 1105 24-25 0.22391796761005622 1105 26-27 0.23234151755395516 1105 28-29 0.22136451708541216 1105 30-31 0.25546133548159133 1105 32-33 0.2315618357125615 1105 34-35 0.23509551841825527 1105 36-37 0.3208090536538748 1105 38-39 0.23914919266321988 1105 40-41 0.24227730790219937 1105 42-43 0.12439874310963006 1105 44-45 0.014704570275917206 1105 46-47 0.04885624677975642 1105 48-49 0.03987174833700635 1105 50-51 -0.006335792628568981 1105 52-53 0.0707392496868593 1105 54-55 0.1121970950056621 1105 56-57 -0.07541407150102941 1105 58-59 -0.22350737647455787 1105 60-61 -0.08993054924523847 1105 62-63 0.004212693236958387 1105 64-65 0.11401706532407019 1105 66-67 0.061542668985275384 1105 68-69 -0.05283874022063628 1105 70-71 -0.1497919223800679 1105 72-73 -0.22089989112729924 1105 74-75 -0.1383726564100627 1105 76-77 -0.3823347721112711 1105 78-79 -0.35425999851834433 1105 80-81 -0.1821351848903241 1105 82-83 -0.2821124953584757 1105 84-85 -0.23945844521050574 1105 86-87 -0.21026319903918989 1105 88-89 -0.29477786109930904 1105 90-91 -0.32296903731096194 1105 92-93 -0.03942072628470683 1105 94-95 -0.045577566083263577 1105 96-97 -0.27375910264163394 1105 98-99 -0.280635506496683 1105 100-101 -0.882104570572146 1106 1 -0.11076879187020694 1106 2 -0.07694852019058374 1106 3 -0.0729302096359703 1106 4 -0.02454976816567722 1106 5 -0.035203713711815965 1106 6 -0.015233868402049211 1106 7 -0.01227354314282536 1106 8 -0.021506556551194933 1106 9 0.016730003218022205 1106 10-11 0.01881970391155363 1106 12-13 0.07917719141192237 1106 14-15 0.029389590581565983 1106 16-17 -0.033667500139905826 1106 18-19 -0.02009852613991825 1106 20-21 -0.07306931733103283 1106 22-23 0.04683116034867396 1106 24-25 0.08055887392030314 1106 26-27 0.14950175768280616 1106 28-29 0.07900918678240743 1106 30-31 0.13353215452153933 1106 32-33 0.0443260858616199 1106 34-35 0.08770685194260608 1106 36-37 0.05554642965957868 1106 38-39 0.1037079239527614 1106 40-41 0.24287138676296394 1106 42-43 0.3109532986660426 1106 44-45 0.5203656215462544 1106 46-47 0.36995889586330577 1106 48-49 0.21485011467095916 1106 50-51 0.3758940624113478 1106 52-53 0.3572064962196677 1106 54-55 0.2655732536409303 1106 56-57 0.2624970165513787 1106 58-59 0.22791899356082723 1106 60-61 0.35866795701546295 1106 62-63 0.35476737656266977 1106 64-65 0.4833064066539592 1106 66-67 0.3362607053646798 1106 68-69 0.0444418547197003 1106 70-71 0.07392244740752574 1106 72-73 0.09546222643594149 1106 74-75 0.15511077036263643 1106 76-77 0.05888384697359328 1106 78-79 0.1704147221314969 1106 80-81 0.44472860141744874 1106 82-83 0.29300230342423106 1106 84-85 0.10423371982097507 1106 86-87 0.0813963693328752 1106 88-89 0.289565123978786 1106 90-91 0.41708480002710147 1106 92-93 0.4522919341044833 1106 94-95 0.5526312922012195 1106 96-97 0.29737403684372765 1106 98-99 0.09076662608913821 1106 100-101 -0.6255406646258415 1107 1 -0.05357808354852267 1107 2 -0.03436571340501615 1107 3 -0.04128638004013396 1107 4 0.012284418148539089 1107 5 0.024491700576930953 1107 6 0.043989312728818675 1107 7 0.061152634658107274 1107 8 0.09426163588054237 1107 9 0.018033086199203296 1107 10-11 0.01914138352800876 1107 12-13 0.0177078636568595 1107 14-15 0.07027773491097378 1107 16-17 0.14328364102788527 1107 18-19 0.14696938285841554 1107 20-21 0.09531640271232078 1107 22-23 -0.0018516364311622624 1107 24-25 0.08404404813693844 1107 26-27 0.08228310812002348 1107 28-29 0.17115146081416555 1107 30-31 0.14657490705172904 1107 32-33 0.22802010869900613 1107 34-35 0.1678798856525674 1107 36-37 0.30689369683226886 1107 38-39 0.2844589055899007 1107 40-41 0.34654386454695896 1107 42-43 0.2458127087766968 1107 44-45 0.3357058585231343 1107 46-47 0.4321040781032224 1107 48-49 0.342165342927899 1107 50-51 0.18920860616398016 1107 52-53 0.3176410722898808 1107 54-55 0.09686819176904038 1107 56-57 0.19889492488578497 1107 58-59 0.22319806465483083 1107 60-61 0.14505165159936695 1107 62-63 0.14506756723265113 1107 64-65 0.05954050151390078 1107 66-67 0.1425717040957295 1107 68-69 0.21833587063722604 1107 70-71 0.4402748530380478 1107 72-73 0.1122844806806711 1107 74-75 0.14213698257580276 1107 76-77 0.18461358746978362 1107 78-79 0.2314641831778701 1107 80-81 0.2145296650174373 1107 82-83 0.3068261108952406 1107 84-85 0.3204784001845873 1107 86-87 0.3611449542698537 1107 88-89 -0.08810708449204796 1107 90-91 0.4505348045624089 1107 92-93 0.6060954682787347 1107 94-95 0.9433051081350214 1107 96-97 0.5886261747507717 1107 98-99 0.26238737704272097 1107 100-101 -0.6649401056139972 1108 1 -0.27835505837805385 1108 2 -0.130933814913206 1108 3 -0.11396715032444149 1108 4 -0.10296974818824367 1108 5 -0.14232057060784342 1108 6 0.005114271436291062 1108 7 -0.08741402536535503 1108 8 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-0.788131035219763 2316 94-95 -0.7920065786941741 2316 96-97 -0.8208418899318985 2316 98-99 -0.6500551253765217 2316 100-101 0.2903723076369751 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 8.0 5 29.0 6 56.0 7 156.0 8 379.0 9 794.0 10 1405.0 11 2444.0 12 3635.0 13 5324.0 14 6767.0 15 9807.0 16 13653.0 17 20494.0 18 19690.0 19 10861.0 20 8894.0 21 9023.0 22 9282.0 23 10087.0 24 11174.0 25 13153.0 26 15168.0 27 18390.0 28 21741.0 29 26849.0 30 33094.0 31 41384.0 32 51788.0 33 68163.0 34 96537.0 35 144887.0 36 234696.0 37 376388.0 38 262861.0 39 11846.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18292671940615 20.658612892473492 15.730534460192501 20.42792592792786 2 15.29226805081385 22.27049169300275 39.768071634998236 22.669168621185165 3 18.711805206311947 27.976767443156664 31.251133150918726 22.060294199612663 4 12.582571866593462 18.829428674115725 38.47350877339096 30.114490685899852 5 12.92134795469062 39.09641344520803 33.200633219148024 14.781605380953328 6 27.901106595947372 38.079508595290065 18.853004619732168 15.166380189030395 7 26.6926471878958 35.448996837107515 21.031857637164915 16.82649833783177 8 26.433503981323753 33.96492949959415 20.135587313322397 19.465979205759705 9 26.924626804953256 17.537748740640893 21.337832409621804 34.19979204478405 10-11 23.96033603410312 28.063799840221716 26.927477700550895 21.04838642512427 12-13 26.66557969598562 25.570002940586694 27.68956373609548 20.07485362733221 14-15 23.657854206814548 26.200496381565458 27.557409334126042 22.584240077493952 16-17 20.696061808663888 30.028801033262294 27.89632012423749 21.378817033836327 18-19 20.58971255925218 27.91901278290334 31.453322510607574 20.037952147236908 20-21 22.451956002672794 26.409896003172502 30.924145035629792 20.214002958524915 22-23 21.79125293978588 26.663946036710566 30.676853866935573 20.867947156567983 24-25 21.350569843325516 26.916676570068194 31.282330868854512 20.450422717751778 26-27 21.40924820939313 26.82401006885015 31.28064228961911 20.486099432137607 28-29 20.727484491448276 26.861010190830626 31.780727048848973 20.630778268872128 30-31 20.954338902936932 27.10564265913603 31.370998896158852 20.56901954176818 32-33 20.84405088560349 27.112209328771048 31.013804100548846 21.029935685076616 34-35 21.23269348996185 26.904272738136008 30.949391643154556 20.91364212874759 36-37 21.683384198848117 26.523794647505987 31.02764360496319 20.765177548682704 38-39 21.478904601496474 26.69378656043409 31.090041592950406 20.73726724511903 40-41 21.031329698165298 26.858319747637626 31.332837638309 20.77751291588807 42-43 21.027274824126398 27.141845743794768 30.569683107164362 21.261196324914465 44-45 21.129124742528933 27.287928731368382 30.09683795099481 21.486108575107874 46-47 21.331336106002542 27.30732132669114 29.480170973081314 21.881171594224995 48-49 21.595749792582957 27.4280286892204 29.20451160093922 21.771709917257418 50-51 21.909536309923265 27.661112721646614 28.849976215682915 21.579374752747206 52-53 21.676448335044938 27.89985732708998 28.794750251935884 21.6289440859292 54-55 21.182154290469743 27.72922001418401 28.938896658558168 22.149729036788084 56-57 21.620219980440915 28.137918133090828 28.230748508163337 22.01111337830492 58-59 21.68461427824142 27.41267697086045 28.72907718086945 22.17363157002868 60-61 21.646489910767826 27.57035810751939 28.98190883044862 21.801243151264167 62-63 22.01201874565554 27.412766393639547 28.952491999782175 21.622722860922742 64-65 21.654908766962944 27.396306728542598 29.10138637826257 21.84739812623189 66-67 21.351893316932383 27.67167344166931 28.85632189430996 22.12011134708835 68-69 21.220958727966178 27.80084941496208 28.991779421195908 21.98641243587583 70-71 21.35541577228902 27.836224865198695 28.592322996511328 22.216036366000953 72-73 21.284594336775978 27.731750584433595 28.345301756087622 22.6383533227028 74-75 21.147431022741177 27.99742842810586 28.309521414710492 22.54561913444247 76-77 21.086822547556583 27.559923891511417 28.883710102843935 22.469543458088065 78-79 20.885630868705356 27.475080278458847 28.914213620314204 22.725075232521593 80-81 20.71315724733872 28.30401485293138 28.46020639321829 22.522621506511612 82-83 20.959303945831703 27.86417308331726 28.34001638784481 22.836506583006226 84-85 20.715632453570443 27.475458273459186 28.338286630965335 23.470622642005033 86-87 20.762207352389904 27.371315860443058 28.56561986025835 23.300856926908686 88-89 20.972844128773257 26.978306183895622 28.68479631488201 23.364053372449114 90-91 20.571199336523964 27.278213736180128 28.826592572504158 23.32399435479175 92-93 20.512450085302643 27.41193775694097 28.60034479820464 23.47526735955175 94-95 20.18915217861347 27.1090467915402 28.581582888214424 24.120218141631895 96-97 20.194559209898312 26.911493465683222 28.442168141337792 24.45177918308067 98-99 20.1266953657467 26.97702845680675 28.39424707197951 24.502029105467038 100-101 20.45720198025828 25.978188970198424 29.28341365519738 24.281195394345918 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 940.0 1 630.0 2 362.0 3 412.0 4 347.5 5 343.0 6 704.5 7 1430.0 8 2330.5 9 2924.5 10 2635.0 11 2170.0 12 2254.0 13 2414.0 14 2583.5 15 2753.0 16 2862.0 17 3023.5 18 3191.5 19 3342.5 20 3638.0 21 4083.0 22 4551.5 23 5283.0 24 6191.5 25 7580.0 26 9770.5 27 11829.5 28 13583.0 29 16198.5 30 19493.5 31 22934.0 32 27143.0 33 32136.5 34 37185.0 35 41286.5 36 47222.0 37 52839.0 38 54076.0 39 53525.0 40 52552.0 41 52589.5 42 52362.0 43 53000.0 44 55772.5 45 58017.5 46 57163.0 47 57438.0 48 72654.5 49 72615.0 50 56234.0 51 49021.5 52 43225.0 53 40771.5 54 38719.5 55 35452.0 56 31724.0 57 28411.5 58 26232.5 59 23676.0 60 20464.0 61 17425.5 62 14483.0 63 10970.0 64 7806.5 65 5996.5 66 4310.0 67 3340.5 68 2563.5 69 1666.0 70 1259.0 71 1024.0 72 880.5 73 871.5 74 785.0 75 645.5 76 450.5 77 258.0 78 153.5 79 90.0 80 46.5 81 27.0 82 15.0 83 9.5 84 4.5 85 1.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4394223124543136 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010891061833683234 16-17 0.0020180496927118935 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.609760441485208E-5 26-27 0.0038118716417891324 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012332525899906016 36-37 0.02725968711901304 38-39 0.019988301718289234 40-41 0.009065207349801046 42-43 0.008520654258116885 44-45 0.0037157740373742806 46-47 0.005285368242816864 48-49 0.0030751233412752666 50-51 6.726832309039646E-4 52-53 0.0 54-55 0.0 56-57 9.609760441485208E-5 58-59 0.001761789414272288 60-61 0.0026266678540059567 62-63 0.0011531712529782249 64-65 0.003043090806470316 66-67 0.0017938219490772386 68-69 0.0047408151511327025 70-71 2.882928132445562E-4 72-73 0.0 74-75 0.0 76-77 0.09994150859144615 78-79 0.016112365006890197 80-81 5.765856264891124E-4 82-83 0.0 84-85 0.0 86-87 0.10753321934021946 88-89 0.4198184011536838 90-91 0.4276023071112868 92-93 0.0 94-95 3.203253480495069E-5 96-97 0.0 98-99 2.2422774363465482E-4 100-101 3.523578828544576E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1560913.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.06424007688306 #Duplication Level Percentage of deduplicated Percentage of total 1 73.25465100834434 33.74419830796658 2 14.101701639812164 12.991683396577663 3 5.1113165343266544 7.063467358384948 4 2.3744477601410097 4.375085266926109 5 1.2984931999814335 2.9907051251072443 6 0.7933818016128528 2.192791786927468 7 0.5385278039850935 1.7364811835592175 8 0.37024313904518924 1.3643975075037116 9 0.2614038949005103 1.0837234594556477 >10 1.6249352541934585 14.333685345241387 >50 0.16660955678239353 5.387790008077886 >100 0.09803117895102174 8.024190685452517 >500 0.0036152872449391198 1.098749720746202 >1k 0.002224792150731766 2.070996544297945 >5k 2.780990188414707E-4 0.8928222197435766 >10k+ 1.3904950942073536E-4 0.649232084031917 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10136 0.6493635455659604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8518 0.54570626293714 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5421 0.3472967423552754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4920 0.3152001424807148 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4351 0.2787471178726809 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3386 0.21692432569912606 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2105 0.1348569715288424 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2009 0.1287067248462919 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1925 0.12332525899906017 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1894 0.12133924184115322 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1677 0.10743712173580462 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1599 0.10244004630623231 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2813013921980277E-4 0.0 3 0.0 0.0 0.0 1.2813013921980277E-4 0.0 4 0.0 0.0 0.0 1.2813013921980277E-4 0.0 5 0.0 0.0 0.0 1.2813013921980277E-4 0.0 6 0.0 0.0 0.0 1.2813013921980277E-4 0.0 7 0.0 0.0 0.0 1.2813013921980277E-4 0.0 8 0.0 0.0 0.0 1.2813013921980277E-4 0.0 9 0.0 0.0 0.0 1.2813013921980277E-4 0.0 10-11 0.0 0.0 0.0 1.2813013921980277E-4 0.0 12-13 0.0 0.0 0.0 1.2813013921980277E-4 0.0 14-15 0.0 0.0 0.0 1.2813013921980277E-4 0.0 16-17 0.0 0.0 0.0 1.2813013921980277E-4 0.0 18-19 0.0 0.0 0.0 1.2813013921980277E-4 0.0 20-21 0.0 0.0 0.0 2.5626027843960554E-4 0.0 22-23 0.0 0.0 0.0 5.445530916841617E-4 0.0 24-25 0.0 0.0 0.0 0.0016016267402475346 0.0 26-27 0.0 0.0 0.0 0.0033634161545198224 0.0 28-29 0.0 0.0 0.0 0.008680816932141637 0.0 30-31 0.0 0.0 0.0 0.021045375366852605 0.0 32-33 0.0 0.0 0.0 0.036100666725179434 0.0 34-35 0.0 0.0 0.0 0.06076571852499146 0.0 36-37 0.0 0.0 0.0 0.10080638703117983 0.0 38-39 0.0 0.0 0.0 0.16009860895514355 0.0 40-41 0.0 0.0 0.0 0.2327163653579668 0.0 42-43 0.0 0.0 0.0 0.3083131474976504 0.0 44-45 0.0 0.0 0.0 0.4006309128055183 0.0 46-47 0.0 0.0 0.0 0.5172934045651487 0.0 48-49 0.0 0.0 0.0 0.6479541140345426 0.0 50-51 0.0 0.0 0.0 0.792516943609285 0.0 52-53 0.0 0.0 0.0 0.9386813999242751 0.0 54-55 0.0 0.0 0.0 1.0923094368488186 0.0 56-57 0.0 0.0 0.0 1.280564643897514 0.0 58-59 0.0 0.0 0.0 1.5081878362214933 0.0 60-61 0.0 0.0 0.0 1.7699577106475504 0.0 62-63 0.0 0.0 0.0 2.0387427101958915 0.0 64-65 0.0 0.0 0.0 2.3013133979920726 0.0 66-67 0.0 0.0 0.0 2.5963010110108637 0.0 68-69 0.0 0.0 0.0 2.9561865395444844 0.0 70-71 0.0 0.0 0.0 3.367003798417977 0.0 72-73 0.0 0.0 0.0 3.782369677233773 0.0 74-75 0.0 0.0 0.0 4.196422222122566 0.0 76-77 0.0 0.0 0.0 4.6390157555225695 0.0 78-79 0.0 0.0 0.0 5.150895661705681 0.0 80-81 0.0 0.0 0.0 5.712393964301661 0.0 82-83 0.0 0.0 0.0 6.299486262206798 0.0 84-85 0.0 0.0 0.0 6.89644458083186 0.0 86-87 0.0 0.0 0.0 7.516530389586094 0.0 88-89 0.0 0.0 0.0 8.172620767461094 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACGG 140 1.7931778E-5 23.727394 5 GAGTACT 8690 0.0 23.620447 12-13 AGTACTT 9050 0.0 22.497303 12-13 GTACTTT 9330 0.0 21.898443 14-15 GTATCAA 21095 0.0 21.732105 1 CATTCCG 330 1.8189894E-12 21.57174 9 GTACATG 13130 0.0 21.522675 1 TACATGG 13400 0.0 21.000515 2 GGTATCA 16330 0.0 20.135813 1 ACATGGG 13495 0.0 19.832901 3 TTTACGC 120 0.0047905683 19.772827 3 TTCGCCG 60 0.004791622 19.772827 92-93 ACTTTTT 10410 0.0 19.602194 16-17 TACTTTT 10305 0.0 19.458101 14-15 ATGGGAT 3310 0.0 18.781199 5 GTACACG 205 1.5460495E-5 18.59698 1 ATCAACG 24695 0.0 18.524567 3 CATGGGA 9085 0.0 18.490913 4 TCAACGC 24725 0.0 18.482895 4 TATCAAC 24855 0.0 18.462595 2 >>END_MODULE