FastQCFastQC Report
Fri 27 May 2016
SRR939111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975982
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67400.34109622456075006No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62150.31452715662389635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57020.28856538166845647No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40910.20703629891365405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35360.1789489985232659No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33040.16720800088259913No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28740.1454466690486047No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25020.12662058662477696No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24800.12550721615885166No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24560.12429263019602406No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24450.12373594496306141No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23580.11933307084781136No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20770.10511229353303826No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20750.10501107803613596No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20650.10450500055162447No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19800.10020334193327671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG12600.027.070552
TAAGGTG13400.025.45445
GTAAGGT14350.024.4295224
GGTAAGG14900.023.8457033
TAAGGTA6600.022.9689524
TACATGG80900.021.8421522
GTACATG84650.021.7597711
GAGTACT72450.021.43959412-13
GTGTAGC12450.021.3534561
ACATGGG81200.021.236383
GTATTAG4750.020.9882011
AAGGTAA14600.020.8102191
AGTACTT77300.020.15568212-13
GTACTTT76500.020.15081814-15
TATTCCG1656.4567896E-520.0978345
GGTGTGC18700.020.006298
TATATCG1200.004838773519.7389435
AAGGTGT18700.019.2465576
GTACTAG3003.747118E-918.9893231
CATGGGG37850.018.899324
Produced by FastQC (version 0.11.5)