##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939111_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1975982 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16192353978933 33.0 31.0 34.0 30.0 34.0 2 32.254703737179796 34.0 31.0 34.0 30.0 34.0 3 32.30707921428434 34.0 31.0 34.0 30.0 34.0 4 35.747723410435924 37.0 35.0 37.0 35.0 37.0 5 35.80595521619124 37.0 35.0 37.0 35.0 37.0 6 35.89141905138812 37.0 37.0 37.0 35.0 37.0 7 35.83017406029002 37.0 36.0 37.0 35.0 37.0 8 35.816659767143626 37.0 36.0 37.0 35.0 37.0 9 37.50996162920512 39.0 38.0 39.0 35.0 39.0 10-11 37.427488458902964 39.0 38.0 39.0 35.0 39.0 12-13 37.34785590152137 39.0 37.5 39.0 35.0 39.0 14-15 38.66112191305386 40.0 38.0 41.0 34.5 41.0 16-17 38.61002149817154 40.0 38.0 41.0 34.0 41.0 18-19 38.56846114994975 40.0 38.0 41.0 34.0 41.0 20-21 38.47183577583196 40.0 38.0 41.0 34.0 41.0 22-23 38.39807093384454 40.0 38.0 41.0 34.0 41.0 24-25 38.42444010117501 40.0 38.0 41.0 34.0 41.0 26-27 38.332401307299364 40.0 38.0 41.0 34.0 41.0 28-29 38.216758047391124 40.0 38.0 41.0 34.0 41.0 30-31 38.03683156020652 40.0 38.0 41.0 33.5 41.0 32-33 37.80725482317147 40.0 38.0 41.0 33.0 41.0 34-35 37.49277549086986 40.0 37.0 41.0 32.5 41.0 36-37 37.22603723110838 40.0 37.0 41.0 31.5 41.0 38-39 36.969392180698 40.0 36.5 41.0 30.5 41.0 40-41 36.81823265596549 40.0 36.5 41.0 30.5 41.0 42-43 36.614688038656226 40.0 36.0 41.0 30.0 41.0 44-45 36.33633580670269 40.0 36.0 41.0 30.0 41.0 46-47 35.95464027506324 39.0 35.0 41.0 28.5 41.0 48-49 35.874591215911884 39.5 35.0 41.0 28.0 41.0 50-51 34.84683084157649 38.0 34.0 40.0 25.5 40.5 52-53 34.98300313464394 38.5 35.0 40.0 26.5 40.5 54-55 35.47419637425847 39.0 35.0 41.0 27.0 41.0 56-57 35.33964681864511 39.0 35.0 41.0 26.0 41.0 58-59 35.138006064832574 39.0 35.0 41.0 26.0 41.0 60-61 34.83116546608218 38.5 35.0 40.5 26.0 41.0 62-63 34.470205700254354 38.0 34.5 40.0 25.0 41.0 64-65 34.10062237409045 37.0 34.0 40.0 23.5 41.0 66-67 33.70353652006952 37.0 34.0 39.5 22.5 41.0 68-69 33.30239572020393 36.0 34.0 39.0 22.0 41.0 70-71 32.87449000041498 35.5 33.0 39.0 20.5 41.0 72-73 32.438682892860356 35.0 33.0 38.5 19.5 40.0 74-75 32.0120046640101 35.0 32.5 37.0 18.0 39.5 76-77 31.589679713681605 35.0 32.0 37.0 16.0 39.0 78-79 31.18121774388633 35.0 32.0 36.0 11.5 39.0 80-81 30.7809851000667 35.0 31.0 36.0 7.0 37.5 82-83 30.363797342283483 35.0 31.0 35.5 4.5 37.0 84-85 30.005479047886062 34.5 31.0 35.0 2.0 37.0 86-87 29.66532944126009 34.0 30.5 35.0 2.0 36.0 88-89 29.268502698911224 34.0 30.0 35.0 2.0 36.0 90-91 28.90769222594133 34.0 29.5 35.0 2.0 36.0 92-93 28.53818076278023 34.0 29.0 35.0 2.0 35.0 94-95 28.108248202665813 34.0 29.0 35.0 2.0 35.0 96-97 27.582108035397084 34.0 28.0 35.0 2.0 35.0 98-99 26.92818684583159 34.0 26.5 35.0 2.0 35.0 100-101 24.399428486696742 31.5 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3149837754479563 1101 2 0.46370535226220255 1101 3 0.3592256925044879 1101 4 0.31530878886473346 1101 5 0.38293404349614946 1101 6 0.40072042286484333 1101 7 0.3250394879466185 1101 8 0.41645121248429007 1101 9 0.45474338219900545 1101 10-11 0.4424303683337669 1101 12-13 0.42453826957019913 1101 14-15 0.49523529786377907 1101 16-17 0.52602460883395 1101 18-19 0.45450908989396765 1101 20-21 0.44497164015105284 1101 22-23 0.4241138637751831 1101 24-25 0.5030062042079848 1101 26-27 0.47457668723118474 1101 28-29 0.4433521608632347 1101 30-31 0.42241469067594295 1101 32-33 0.3790849553482616 1101 34-35 0.39465159447938447 1101 36-37 0.2990513293157093 1101 38-39 0.32464807369964177 1101 40-41 0.23588219937528976 1101 42-43 0.12797648465046052 1101 44-45 0.19557988272037363 1101 46-47 0.11270771477136066 1101 48-49 0.12625210951892285 1101 50-51 0.07574134776640307 1101 52-53 0.24917035931026987 1101 54-55 0.1927848195109121 1101 56-57 0.1342280765730237 1101 58-59 0.1896749605288761 1101 60-61 -0.05189388508452453 1101 62-63 0.18935644332213286 1101 64-65 0.13133716176568555 1101 66-67 0.015887417415342497 1101 68-69 -0.1240987993820184 1101 70-71 -0.09509111829338224 1101 72-73 -0.19311224939726657 1101 74-75 -0.15737073935240176 1101 76-77 -0.28588403887513536 1101 78-79 -0.243981524741681 1101 80-81 -0.37143538706074963 1101 82-83 -0.3742962887384351 1101 84-85 -0.5022456721888844 1101 86-87 -0.4966147634208973 1101 88-89 -0.49325003170817894 1101 90-91 -0.6233909230446528 1101 92-93 -0.6433323584371777 1101 94-95 -0.8956803061557075 1101 96-97 -0.7684963784802932 1101 98-99 -0.7776159604926001 1101 100-101 -0.9795493561803603 1102 1 0.19694534557049082 1102 2 0.30370139377236427 1102 3 0.3620834868713203 1102 4 0.38724984335244983 1102 5 0.31996844948564984 1102 6 0.3943290534799573 1102 7 0.2897698279372989 1102 8 0.39618505940630655 1102 9 0.5052944046762349 1102 10-11 0.32297290289529457 1102 12-13 0.3835270691892987 1102 14-15 0.45431458219373155 1102 16-17 0.5706233636397684 1102 18-19 0.4751428911757003 1102 20-21 0.5564109145294154 1102 22-23 0.5872444756006132 1102 24-25 0.53955787513015 1102 26-27 0.45343682901016535 1102 28-29 0.5090444069636462 1102 30-31 0.43622736344897106 1102 32-33 0.524250286312963 1102 34-35 0.4879935806655027 1102 36-37 0.4894323720248437 1102 38-39 0.4653095844102282 1102 40-41 0.5330016367714663 1102 42-43 0.5747835854338135 1102 44-45 0.5915055101704212 1102 46-47 0.4897350134722913 1102 48-49 0.470351797026602 1102 50-51 0.5835514066904608 1102 52-53 0.5465986211975888 1102 54-55 0.5858426179608003 1102 56-57 0.4912295343630433 1102 58-59 0.5633984427127245 1102 60-61 0.511097660563891 1102 62-63 0.531424463603166 1102 64-65 0.4510966350650847 1102 66-67 0.5761817228301354 1102 68-69 0.4556530060734403 1102 70-71 0.3301897908859246 1102 72-73 0.22086879760252742 1102 74-75 0.28996733194263413 1102 76-77 0.23851724818769426 1102 78-79 0.30986656682676994 1102 80-81 0.27585728616902827 1102 82-83 0.22018670446119515 1102 84-85 0.26833683135524566 1102 86-87 0.12277090248602107 1102 88-89 0.015347745931958201 1102 90-91 -0.15268305578831587 1102 92-93 -0.2871907649166445 1102 94-95 -0.35891548578083743 1102 96-97 -0.6507543163077827 1102 98-99 -0.9582173879710929 1102 100-101 -0.9417921451717426 1103 1 0.3195507433868201 1103 2 0.40995793353633303 1103 3 0.43207496586239813 1103 4 0.3045607365572707 1103 5 0.3033723497236167 1103 6 0.29774143057654356 1103 7 0.31134608213709214 1103 8 0.31866595336168757 1103 9 0.42436248003198074 1103 10-11 0.4936204554231196 1103 12-13 0.5669186073417833 1103 14-15 0.5230919277890749 1103 16-17 0.5002345319009933 1103 18-19 0.4629397467909584 1103 20-21 0.4678167044004553 1103 22-23 0.5437365572433563 1103 24-25 0.559582246098671 1103 26-27 0.4368826720381733 1103 28-29 0.46330248315825173 1103 30-31 0.3345734930124564 1103 32-33 0.4763405825173592 1103 34-35 0.3978099595457607 1103 36-37 0.18453477865797652 1103 38-39 0.26512327012186176 1103 40-41 0.18863146295171163 1103 42-43 0.08346132794039818 1103 44-45 -0.03274448651830397 1103 46-47 -0.001666714570660588 1103 48-49 0.1320412526091843 1103 50-51 0.15792921851303277 1103 52-53 0.07630073908773483 1103 54-55 0.08348057662728081 1103 56-57 0.03264252073383744 1103 58-59 0.02950866836494015 1103 60-61 -0.03755156084547906 1103 62-63 -0.01602828453258809 1103 64-65 -0.03247503633302529 1103 66-67 -0.00437492421907848 1103 68-69 -0.058342640706463555 1103 70-71 -0.06895766671874526 1103 72-73 -0.045019793517077744 1103 74-75 -0.10684678202696674 1103 76-77 -0.1643520801537086 1103 78-79 -0.0449738518947278 1103 80-81 -0.10983315838039687 1103 82-83 -0.21785275708167973 1103 84-85 -0.09655219685238592 1103 86-87 -0.006635849861677201 1103 88-89 -0.02385110262807899 1103 90-91 -0.12891473893040484 1103 92-93 -0.1706803167468962 1103 94-95 -0.24814457730000328 1103 96-97 -0.3012251793137999 1103 98-99 -0.32109536977234043 1103 100-101 -0.5549670377761267 1104 1 0.06734814306054915 1104 2 0.16714831808688047 1104 3 0.19110290393800966 1104 4 0.10341216834474665 1104 5 0.24008600779082911 1104 6 0.14381163368003058 1104 7 0.1342147405025642 1104 8 0.0958557802918989 1104 9 0.20882503777161787 1104 10-11 0.21607222774803603 1104 12-13 0.20945414591225386 1104 14-15 0.23922313844766308 1104 16-17 0.31991438002077643 1104 18-19 0.3498402400904368 1104 20-21 0.3466396456791401 1104 22-23 0.3230857040842423 1104 24-25 0.32396398779336977 1104 26-27 0.3669437898205601 1104 28-29 0.3638518688681245 1104 30-31 0.13812838749253586 1104 32-33 0.1937655047747171 1104 34-35 0.11028519082852029 1104 36-37 -0.07571084661596927 1104 38-39 0.1190286873385844 1104 40-41 0.038947685553750944 1104 42-43 0.036598201687382925 1104 44-45 -0.03337780705663107 1104 46-47 -0.15670170200475297 1104 48-49 0.1349548188635623 1104 50-51 0.021569649359030052 1104 52-53 0.06982326565250929 1104 54-55 0.14105990136201996 1104 56-57 0.02050357990124496 1104 58-59 0.09370568211352293 1104 60-61 0.09972143580620951 1104 62-63 0.08936017583877032 1104 64-65 -0.02226118245030051 1104 66-67 -0.03771267745003826 1104 68-69 -0.16398040923955648 1104 70-71 -0.27128274900016436 1104 72-73 -0.20998950046813292 1104 74-75 -0.19613606248924498 1104 76-77 -0.0354338247013537 1104 78-79 -0.16301299943588177 1104 80-81 -0.21312730051393203 1104 82-83 -0.22260582204833668 1104 84-85 -0.11904553134418805 1104 86-87 -0.1822650206672698 1104 88-89 -0.1756719923826502 1104 90-91 -0.3110732548303652 1104 92-93 -0.45393637211470406 1104 94-95 -0.40725424565855306 1104 96-97 -0.479979779681571 1104 98-99 -0.42387792766759347 1104 100-101 -0.7128129828388516 1105 1 0.2031429824017934 1105 2 0.27600333709697367 1105 3 0.21777203441793347 1105 4 0.21914258597345082 1105 5 0.23464687221846958 1105 6 0.23547908316361799 1105 7 0.18791021318390477 1105 8 0.2320473842096078 1105 9 0.21699455636393594 1105 10-11 0.2668497503549645 1105 12-13 0.3074376919245836 1105 14-15 0.4383634200639932 1105 16-17 0.35681719371199705 1105 18-19 0.42031261242123463 1105 20-21 0.40141107961845535 1105 22-23 0.36916508913622437 1105 24-25 0.3728111456735306 1105 26-27 0.4472762562871466 1105 28-29 0.4185676816051327 1105 30-31 0.38572678757955003 1105 32-33 0.3432375977634763 1105 34-35 0.3805928007978139 1105 36-37 0.3732072591978053 1105 38-39 0.43292138044304096 1105 40-41 0.3759818888850859 1105 42-43 0.22998508793831718 1105 44-45 0.04472332217738284 1105 46-47 0.09603795121559244 1105 48-49 0.3208686875777431 1105 50-51 0.25124318998229 1105 52-53 0.17924005880590954 1105 54-55 0.2926248965909579 1105 56-57 0.1460709161647884 1105 58-59 0.15386804658318454 1105 60-61 0.3038694796703254 1105 62-63 0.28076540225413993 1105 64-65 0.28967920645314393 1105 66-67 0.23379449644902905 1105 68-69 0.20910838444780921 1105 70-71 0.26678378384600876 1105 72-73 0.24786878659659095 1105 74-75 0.2064254792810445 1105 76-77 0.3456375385372361 1105 78-79 0.22650598840656144 1105 80-81 0.1341427490754583 1105 82-83 0.17346472541261448 1105 84-85 0.3462323151017408 1105 86-87 0.33186863584585424 1105 88-89 0.24228982427998247 1105 90-91 0.19372716393548473 1105 92-93 0.17691466936140898 1105 94-95 0.09131227235530304 1105 96-97 0.27631468637960666 1105 98-99 0.2203154042154658 1105 100-101 0.22461841767830037 1106 1 0.12608988709613556 1106 2 0.13343259795955476 1106 3 0.17976654010449522 1106 4 0.2281299414692768 1106 5 0.1955986787835684 1106 6 0.2591246555136024 1106 7 0.15841456556407252 1106 8 0.2190506728589412 1106 9 0.1363210438245872 1106 10-11 0.07134721973604741 1106 12-13 0.22714437947683308 1106 14-15 0.19675522255308664 1106 16-17 0.1952056429755018 1106 18-19 0.27081355233155335 1106 20-21 0.3115395982096061 1106 22-23 0.275365416520053 1106 24-25 0.2688658921169562 1106 26-27 0.23158964780230207 1106 28-29 0.15034829178554077 1106 30-31 0.08476190580445575 1106 32-33 0.08000879699187635 1106 34-35 0.01695770283446052 1106 36-37 -0.00921464056444421 1106 38-39 0.10412457821166043 1106 40-41 0.12797264440946776 1106 42-43 -0.0017343525351236622 1106 44-45 0.061499669732690165 1106 46-47 -0.04395462547759621 1106 48-49 -0.14400671876388316 1106 50-51 -0.1089902502079596 1106 52-53 -0.12318015841912455 1106 54-55 -0.1262283772769237 1106 56-57 -0.10259342180717823 1106 58-59 -0.15261603122348788 1106 60-61 -0.06756334888209636 1106 62-63 -0.031863194835665354 1106 64-65 -0.14223772478855068 1106 66-67 -0.10772738216301292 1106 68-69 0.06460272750884855 1106 70-71 -0.01569333157426911 1106 72-73 -0.06937997475386481 1106 74-75 -0.0765947579685573 1106 76-77 -0.04174251403612672 1106 78-79 -0.06313420337898279 1106 80-81 -0.08380639801443124 1106 82-83 -0.09224863185935206 1106 84-85 -0.06264970408510706 1106 86-87 -0.16879267829865796 1106 88-89 0.036956892227824056 1106 90-91 0.11384189638540931 1106 92-93 0.1690890939662637 1106 94-95 -0.11947394647998877 1106 96-97 0.05538110927586004 1106 98-99 -0.17153803494392506 1106 100-101 -0.31097627924875937 1107 1 0.09363252567415259 1107 2 0.0807445305558403 1107 3 0.13283018522515988 1107 4 0.11909252332279152 1107 5 0.10857493826644316 1107 6 0.07246351744119295 1107 7 0.15921621173121991 1107 8 0.09547502631664173 1107 9 0.13614192865352948 1107 10-11 0.16319141492362377 1107 12-13 0.2261873121140141 1107 14-15 0.21876114322621731 1107 16-17 0.18962472452368218 1107 18-19 0.20726391274795475 1107 20-21 0.192606419451252 1107 22-23 0.2625021265269538 1107 24-25 0.2891373573777756 1107 26-27 0.19406530153742096 1107 28-29 0.13467063703779303 1107 30-31 0.3049851369011023 1107 32-33 0.18932504245582038 1107 34-35 0.2657777287567953 1107 36-37 0.2661147127451713 1107 38-39 0.45445410781572804 1107 40-41 0.36500167376038206 1107 42-43 0.3946255194610018 1107 44-45 0.3340190303902588 1107 46-47 0.4304783057633301 1107 48-49 0.47247850564486527 1107 50-51 0.3166433873390915 1107 52-53 0.44271617197643565 1107 54-55 0.3096269540790857 1107 56-57 0.31721008743981827 1107 58-59 0.25895114190189616 1107 60-61 0.22221961148267866 1107 62-63 0.15013530736660385 1107 64-65 -0.02260162476048322 1107 66-67 -0.1340959560536632 1107 68-69 0.1199959981377745 1107 70-71 0.06472557226909004 1107 72-73 -0.007371007712471567 1107 74-75 0.23675707923126765 1107 76-77 0.1663925585478303 1107 78-79 0.0686419562507119 1107 80-81 0.08513503132463285 1107 82-83 -0.026689658541872774 1107 84-85 0.010686675616490504 1107 86-87 -0.08014336182753468 1107 88-89 -0.10055194012460689 1107 90-91 0.0761736938414117 1107 92-93 0.07695634000704388 1107 94-95 -0.11650430156523939 1107 96-97 -0.0460935586206368 1107 98-99 0.12112769242585841 1107 100-101 0.09284874638445828 1108 1 0.017250156081267676 1108 2 0.05859743917683602 1108 3 0.073218679498666 1108 4 -0.0066253799913056355 1108 5 0.1258519984197477 1108 6 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sequence quality scores pass #Quality Count 2 5960.0 3 1422.0 4 1421.0 5 1650.0 6 1991.0 7 2238.0 8 2791.0 9 3480.0 10 4327.0 11 5576.0 12 7474.0 13 11426.0 14 10498.0 15 11279.0 16 13951.0 17 18694.0 18 17085.0 19 11327.0 20 10880.0 21 11096.0 22 11739.0 23 13110.0 24 14488.0 25 16117.0 26 18109.0 27 21086.0 28 25074.0 29 30027.0 30 36223.0 31 44195.0 32 55707.0 33 73185.0 34 102410.0 35 157811.0 36 261860.0 37 428851.0 38 427225.0 39 84179.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54557553286507 19.056454221359694 15.17517255246646 22.222797693308777 2 15.255862124177014 20.726063309623726 39.793838879368785 24.224235686830475 3 19.460942990044803 24.640087387263694 30.404262358371888 25.494707264319622 4 12.716800788007212 17.101712314538105 37.862601376377825 32.318885521076865 5 13.487387058210029 37.74973381490748 34.2794201041834 14.483459022699089 6 30.78603582270088 36.793006666420155 17.87275297674072 14.54820453413824 7 28.10008160808969 33.41023034149263 20.65409061182765 17.83559743859004 8 26.79092689579426 33.37771198659319 20.078959553619647 19.7524015639929 9 26.103172283560927 17.470986879195703 20.85024108585128 35.57559975139209 10-11 24.588462071293815 26.54977774659794 27.53125869809729 21.33050148401095 12-13 26.59201774959096 23.961897139499424 27.553909823567853 21.892175287341768 14-15 23.07214582450019 25.480231741163944 26.565486330726067 24.8821361036098 16-17 21.703788664826202 28.689852498843276 27.42448236066683 22.18187647566369 18-19 21.754825623556403 27.20239085784107 29.708470458371867 21.334313060230663 20-21 22.635881990102934 25.888285011152245 29.672880091497824 21.802952907246997 22-23 22.128405466733785 25.91482314588757 29.89195380226398 22.06481758511466 24-25 22.33353755220123 26.167404743046554 29.744528783965325 21.754528920786896 26-27 21.770257523006652 26.148039686905296 29.97045956045833 22.111243229629725 28-29 21.65558813186412 27.2020678129969 29.55764570916642 21.58469834597256 30-31 21.863845409825185 26.38878685178927 29.330393989308313 22.416973749077233 32-33 21.50458562213359 26.418528421231287 29.44218924351484 22.634696713120288 34-35 22.719746448651232 26.431621208420776 28.971019003657343 21.877613339270656 36-37 22.646841638458532 25.807593412316105 29.42454900891811 22.121015940307252 38-39 22.033879346898765 26.3692114647596 29.356202926285626 22.240706262056005 40-41 22.331792543056263 26.385917005734566 29.38764960055264 21.894640850656526 42-43 21.91264748183514 26.52981060849708 28.77968800093357 22.777853908734212 44-45 22.17861673215118 26.48396601975443 28.97669992125586 22.360717326838532 46-47 22.99483611242771 26.90935175258906 28.068759955002882 22.02705217998035 48-49 22.41232513854642 27.031505578477088 27.973407631649614 22.582761651326873 50-51 22.284404250596086 27.22368417372307 28.38923909895418 22.102672476726664 52-53 22.796698846681494 27.071466078755215 27.973752499958675 22.158082574604617 54-55 22.097401702569517 27.019252429499886 28.278432814304388 22.60491305362621 56-57 22.47306968253008 27.36666258265672 27.725936835448124 22.43433089936508 58-59 22.647356171339432 26.76585206921037 27.8171163565185 22.769675402931696 60-61 21.986649076798674 27.09088631950811 28.5546255077982 22.36783909589501 62-63 22.065148115089528 27.04548511018986 28.65561687736381 22.233749897356798 64-65 21.745374038875266 27.42248190150881 28.21635389530797 22.615790164307956 66-67 21.759992553460965 27.242710377443903 28.006234950514084 22.99106211858105 68-69 22.227227371081877 26.909960989513692 28.321989506822703 22.54082213258173 70-71 22.376082153505507 26.95068668535672 27.78410924705082 22.88912191408695 72-73 22.11354279942098 26.815619726175665 28.196923011192844 22.87391446321051 74-75 22.008225072993074 27.886100287902988 27.53970110990163 22.565973529202317 76-77 22.19798722992767 27.132170070654695 27.796050080489955 22.873792618927688 78-79 22.25618560684637 27.350667878773088 27.901530589417256 22.49161592496329 80-81 22.70344640338989 27.395153370505586 27.473275609819964 22.42812461628456 82-83 22.433357792921367 27.3519216032065 27.801881405068574 22.412839198803567 84-85 22.32567576535211 27.629715191152737 27.612349573194813 22.432259470300345 86-87 22.62976950461456 27.62664583713144 27.294675119511748 22.44890953874225 88-89 22.41588404432002 27.66161317811341 26.93686474846525 22.985638029101317 90-91 22.30368537470634 27.3911012124352 27.393263050699684 22.911950362158777 92-93 22.630596886818534 27.215096999861903 27.55514265972113 22.59916345359843 94-95 22.244752836644764 27.057651527212816 27.743980443211914 22.953615192930503 96-97 22.283348037608697 26.680464694098088 27.996906379841313 23.039280888451902 98-99 22.272304767717614 27.19171959538932 27.19716187606096 23.338813760832107 100-101 22.444961426783102 26.365777181855194 27.95953670946138 23.229724681900326 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2085.0 1 1187.5 2 317.0 3 334.0 4 284.0 5 295.0 6 533.5 7 1038.0 8 1634.0 9 1998.5 10 1934.5 11 1755.0 12 1816.5 13 2054.0 14 2245.0 15 2409.5 16 2660.0 17 2846.0 18 3114.5 19 3442.5 20 3771.5 21 4392.5 22 5151.0 23 6182.5 24 7643.0 25 9568.5 26 12555.0 27 16236.5 28 18795.0 29 21440.0 30 25086.0 31 29914.5 32 37183.5 33 40356.0 34 40125.5 35 42630.5 36 46778.5 37 50702.5 38 53444.0 39 54817.0 40 56100.0 41 59329.5 42 60129.5 43 59758.0 44 61451.0 45 65599.5 46 68676.5 47 74020.5 48 89065.0 49 86794.0 50 71182.5 51 65062.5 52 62572.0 53 63747.5 54 65439.5 55 62075.0 56 56387.5 57 50587.5 58 46829.5 59 41947.5 60 33316.0 61 27965.5 62 23998.0 63 17291.5 64 11164.0 65 8228.0 66 6389.5 67 5237.0 68 4192.0 69 3136.5 70 2384.0 71 1640.5 72 1124.0 73 892.0 74 684.5 75 539.0 76 410.5 77 259.5 78 176.0 79 123.5 80 84.0 81 67.0 82 55.5 83 40.5 84 26.0 85 17.0 86 12.5 87 10.0 88 9.5 89 6.5 90 5.5 91 6.0 92 4.5 93 4.0 94 3.5 95 2.5 96 2.0 97 1.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3330495925570172 2 0.12044644131373666 3 0.11331074878212453 4 0.12125616528895505 5 0.13820976102009028 6 0.0890190295255726 7 0.09681262278704968 8 0.10425196180936872 9 0.0905372619791071 10-11 0.08965162638121196 12-13 0.09372555013152954 14-15 0.09529439033351518 16-17 0.08393295080623205 18-19 0.09931770633538159 20-21 0.10954047152251388 22-23 0.1181943965076605 24-25 0.13203561570904998 26-27 0.13084633362044795 28-29 0.12631694013407005 30-31 0.11634721368919353 32-33 0.10878135529574662 34-35 0.32991191215304594 36-37 0.48816234155979155 38-39 0.4615426658744867 40-41 0.4403633231476805 42-43 0.47275228215641646 44-45 0.48009040568183314 46-47 0.4875550485783777 48-49 0.4986887532376307 50-51 0.4869730594711895 52-53 0.49086985610192807 54-55 0.47697802915208737 56-57 0.4534960338707539 58-59 0.4346699514469261 60-61 0.3949175650385479 62-63 0.46647692134847385 64-65 0.495753503827464 66-67 0.5044580365610618 68-69 0.5176413550325863 70-71 0.5087343913051839 72-73 0.5012191406601882 74-75 0.508203009946447 76-77 0.4852017882753992 78-79 0.4634657603156304 80-81 0.46521172763719504 82-83 0.4794072010777426 84-85 0.478369742234494 86-87 0.4829750473435487 88-89 0.4950702992233735 90-91 0.5092404687896954 92-93 0.5021553840065345 94-95 0.5068366007382659 96-97 0.5061027883857242 98-99 0.5007383670499023 100-101 0.48869372291852864 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1975982.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.99304705102326 #Duplication Level Percentage of deduplicated Percentage of total 1 72.89518963451526 35.713574555570695 2 14.425901238738978 14.13537716285907 3 5.313824271325948 7.810213276378248 4 2.500517814554723 4.900319477616057 5 1.3609859777644173 3.3339425022197493 6 0.815584808636015 2.3974790942162456 7 0.548488700594167 1.881049290061524 8 0.35365934386800163 1.3861479099327216 9 0.2470756188523581 1.0894488677634844 >10 1.3790961236607349 12.33655208261406 >50 0.09561741397867239 3.2145103834931312 >100 0.05317702004974339 4.8176054987341095 >500 0.005803751179189141 1.9601419144935655 >1k 0.004767367040048223 4.076317299695833 >5k 3.109152417422754E-4 0.9473206843515297 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6740 0.34109622456075006 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6215 0.31452715662389635 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5702 0.28856538166845647 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4091 0.20703629891365405 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3536 0.1789489985232659 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3304 0.16720800088259913 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2874 0.1454466690486047 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2502 0.12662058662477696 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2480 0.12550721615885166 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2456 0.12429263019602406 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2445 0.12373594496306141 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2358 0.11933307084781136 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2077 0.10511229353303826 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2075 0.10501107803613596 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2065 0.10450500055162447 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1980 0.10020334193327671 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.060774845114986E-5 2 0.0 0.0 0.0 5.060774845114986E-5 5.060774845114986E-5 3 0.0 0.0 0.0 5.060774845114986E-5 5.060774845114986E-5 4 0.0 0.0 0.0 5.060774845114986E-5 5.060774845114986E-5 5 0.0 0.0 0.0 5.060774845114986E-5 5.060774845114986E-5 6 0.0 0.0 0.0 5.060774845114986E-5 5.060774845114986E-5 7 0.0 0.0 0.0 5.060774845114986E-5 5.060774845114986E-5 8 0.0 0.0 0.0 5.060774845114986E-5 1.0121549690229972E-4 9 0.0 0.0 0.0 5.060774845114986E-5 1.0121549690229972E-4 10-11 0.0 0.0 0.0 5.060774845114986E-5 1.0121549690229972E-4 12-13 0.0 0.0 0.0 5.060774845114986E-5 2.0243099380459943E-4 14-15 0.0 0.0 0.0 5.060774845114986E-5 3.2895036493247405E-4 16-17 0.0 0.0 0.0 1.5182324535344957E-4 4.0486198760919886E-4 18-19 0.0 0.0 0.0 2.2773486803017438E-4 4.0486198760919886E-4 20-21 0.0 0.0 0.0 3.2895036493247405E-4 4.3016586183477383E-4 22-23 0.0 0.0 0.0 6.072929814137983E-4 5.060774845114986E-4 24-25 0.0 0.0 0.0 6.325968556393732E-4 5.566852329626484E-4 26-27 0.0 0.0 0.0 0.0011892820886020218 6.325968556393732E-4 28-29 0.0 0.0 0.0 0.0029099455359411167 7.08508478316098E-4 30-31 0.0 0.0 0.0 0.008249062997537426 7.591162267672479E-4 32-33 0.0 0.0 0.0 0.01811757394551165 8.603317236695476E-4 34-35 0.0 0.0 0.0 0.032768517122119534 9.109394721206975E-4 36-37 0.0 0.0 0.0 0.055415484554009097 9.109394721206975E-4 38-39 0.0 0.0 0.0 0.09197958280996488 9.109394721206975E-4 40-41 0.0 0.0 0.0 0.13390810240174253 9.109394721206975E-4 42-43 0.0 0.0 0.0 0.1757354064966179 9.109394721206975E-4 44-45 0.0 0.0 0.0 0.2336559745989589 0.0010121549690229972 46-47 0.0 0.0 0.0 0.30341875583886896 0.0011639782143764467 48-49 0.0 0.0 0.0 0.3783688312950219 0.0012145859628275965 50-51 0.0 0.0 0.0 0.464680346278458 0.0012651937112787464 52-53 0.0 0.0 0.0 0.5495748443052619 0.0012651937112787464 54-55 0.0 0.0 0.0 0.646716417457244 0.0012651937112787464 56-57 0.0 0.0 0.0 0.7636456202536257 0.0013158014597298962 58-59 0.0 0.0 0.0 0.8951245507297131 0.0013664092081810463 60-61 0.0 0.0 0.0 1.0426714413390406 0.0013917130824066212 62-63 0.0 0.0 0.0 1.191053359797812 0.001417016956632196 64-65 0.0 0.0 0.0 1.3487471039715948 0.001467624705083346 66-67 0.0 0.0 0.0 1.5413601945766713 0.0014929285793089208 68-69 0.0 0.0 0.0 1.7606941763639548 0.0015182324535344958 70-71 0.0 0.0 0.0 2.0010809815069166 0.0015182324535344958 72-73 0.0 0.0 0.0 2.2557138678388773 0.0016194479504367954 74-75 0.0 0.0 0.0 2.5197597953827513 0.0017206634473390951 76-77 0.0 0.0 0.0 2.808249265428531 0.0017206634473390951 78-79 0.0 0.0 0.0 3.1558232817910286 0.0017206634473390951 80-81 0.0 0.0 0.0 3.5366212850117056 0.0017206634473390951 82-83 0.0 0.0 0.0 3.925086362122732 0.0018724866926925448 84-85 0.0 0.0 0.0 4.335110340073948 0.0018724866926925448 86-87 0.0 0.0 0.0 4.763201284222225 0.0020243099380459944 88-89 0.0 0.0 0.0 5.223402844762756 0.0022267409318505937 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 1260 0.0 27.07055 2 TAAGGTG 1340 0.0 25.4544 5 GTAAGGT 1435 0.0 24.429522 4 GGTAAGG 1490 0.0 23.845703 3 TAAGGTA 660 0.0 22.968952 4 TACATGG 8090 0.0 21.842152 2 GTACATG 8465 0.0 21.759771 1 GAGTACT 7245 0.0 21.439594 12-13 GTGTAGC 1245 0.0 21.353456 1 ACATGGG 8120 0.0 21.23638 3 GTATTAG 475 0.0 20.988201 1 AAGGTAA 1460 0.0 20.810219 1 AGTACTT 7730 0.0 20.155682 12-13 GTACTTT 7650 0.0 20.150818 14-15 TATTCCG 165 6.4567896E-5 20.097834 5 GGTGTGC 1870 0.0 20.00629 8 TATATCG 120 0.0048387735 19.738943 5 AAGGTGT 1870 0.0 19.246557 6 GTACTAG 300 3.747118E-9 18.989323 1 CATGGGG 3785 0.0 18.89932 4 >>END_MODULE