FastQCFastQC Report
Fri 27 May 2016
SRR939111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975982
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72070.36473004308743706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70060.3545578856487559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61490.31118704522612045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39190.19833176618005627No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35920.1817830324365303No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28860.1460539620300185No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28710.14529484580325125No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24750.1252541774165959No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24620.12459627668673094No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24140.12216710476107577No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23210.11746058415511881No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22470.11371561076973372No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22420.11346257202747799No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22330.11300710229141762No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21240.1074908577102423No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21170.10713660347108425No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA20210.10227825961977385No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT20040.1014179278961043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG13500.024.6018035
GTGTAAG9550.024.433841
TAAGGTA7800.022.5065924
TATTCCG1703.1819563E-622.3276845
GAGTACT83400.021.90379112-13
GTAAGGT15000.021.5094
GGTGTGC18550.020.2062558
TACATGG87100.020.1551572
GTACTTT89900.020.05619214-15
GTACATG89900.019.9701211
AGTACTT89250.019.90985912-13
ACATGGG85250.019.4794313
GTATAGG6400.019.3460541
AGGTAAG15850.019.158142
GGTAAGG16000.018.9785333
AAGGTAA15100.018.6069031
GTATCAA207400.018.1621281
AGAGTAC153900.018.0206510-11
ACCGTGC2654.0574378E-717.9042768
ACTTTTT101200.017.81604216-17
Produced by FastQC (version 0.11.5)