##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939111_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1975982 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.522251214839 34.0 31.0 34.0 31.0 34.0 2 32.72969085750781 34.0 31.0 34.0 31.0 34.0 3 32.81350639833764 34.0 31.0 34.0 31.0 34.0 4 36.26589968936964 37.0 37.0 37.0 35.0 37.0 5 36.23307398549177 37.0 37.0 37.0 35.0 37.0 6 36.2723638170793 37.0 37.0 37.0 35.0 37.0 7 36.23277337546597 37.0 37.0 37.0 35.0 37.0 8 36.218347130692486 37.0 37.0 37.0 35.0 37.0 9 37.96356545758008 39.0 38.0 39.0 35.0 39.0 10-11 37.91146579270459 39.0 38.0 39.0 35.0 39.0 12-13 37.853185150472015 39.0 38.0 39.0 35.0 39.0 14-15 39.208351341257156 40.5 39.0 41.0 36.0 41.0 16-17 39.1610065273874 40.0 39.0 41.0 36.0 41.0 18-19 39.20724227244985 40.5 38.5 41.0 36.0 41.0 20-21 39.27054092598009 41.0 39.0 41.0 36.0 41.0 22-23 39.237264054024784 41.0 39.0 41.0 36.0 41.0 24-25 39.17534142517493 41.0 39.0 41.0 36.0 41.0 26-27 39.027313507916574 40.5 39.0 41.0 35.0 41.0 28-29 38.879964240564945 40.0 38.0 41.0 35.0 41.0 30-31 38.669873510993526 40.0 38.0 41.0 35.0 41.0 32-33 38.498876507984384 40.0 38.0 41.0 34.0 41.0 34-35 38.424611914481005 40.0 38.0 41.0 34.0 41.0 36-37 38.29081565520333 40.0 38.0 41.0 34.0 41.0 38-39 38.13610093614213 40.0 38.0 41.0 34.0 41.0 40-41 37.96197789251117 40.0 38.0 41.0 33.0 41.0 42-43 37.77779858318547 40.0 37.5 41.0 33.0 41.0 44-45 37.54706647125328 40.0 37.0 41.0 33.0 41.0 46-47 37.2293710165376 40.0 37.0 41.0 32.0 41.0 48-49 36.86652105130513 40.0 36.5 41.0 31.0 41.0 50-51 36.58758961367057 40.0 36.0 41.0 30.5 41.0 52-53 36.33901067924708 39.0 36.0 41.0 30.0 41.0 54-55 36.08341017276473 39.0 35.0 41.0 30.0 41.0 56-57 35.7918657659837 39.0 35.0 40.5 29.0 41.0 58-59 35.479997034385946 38.5 35.0 40.0 28.0 41.0 60-61 35.10170563294605 38.0 35.0 40.0 28.0 41.0 62-63 34.715358490107704 37.5 34.0 40.0 27.0 41.0 64-65 34.30043947768755 37.0 34.0 40.0 26.0 41.0 66-67 33.93487314155696 36.0 34.0 39.0 26.0 41.0 68-69 33.470823620862944 36.0 33.0 39.0 25.5 40.0 70-71 33.01655480667334 35.0 33.0 38.5 24.5 40.0 72-73 32.553657877450306 35.0 32.5 37.5 23.0 40.0 74-75 32.084241911110524 35.0 32.0 37.0 21.5 39.0 76-77 30.122054502520772 33.5 29.5 35.0 18.5 37.5 78-79 31.078336492943762 34.5 31.0 36.0 19.5 38.0 80-81 31.12236574017375 35.0 31.0 36.0 19.5 37.0 82-83 30.836982320689156 35.0 31.0 35.0 18.0 37.0 84-85 30.47052857768947 34.0 31.0 35.0 15.0 36.5 86-87 29.957874363228004 34.0 30.5 35.0 7.5 36.0 88-89 29.41365660213504 34.0 30.0 35.0 2.0 36.0 90-91 28.966799039667364 34.0 29.0 35.0 2.0 35.0 92-93 28.719112319849067 34.0 29.0 35.0 2.0 35.0 94-95 28.410974897544612 34.0 29.0 35.0 2.0 35.0 96-97 27.87569218747944 34.0 29.0 35.0 2.0 35.0 98-99 27.125982169878064 33.5 27.0 35.0 2.0 35.0 100-101 23.499209253930452 29.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2599671550777103 1101 2 0.27372435057943534 1101 3 0.2537513534540423 1101 4 0.14132706740206658 1101 5 0.1648881606073047 1101 6 0.17983998166886295 1101 7 0.21402086892530292 1101 8 0.2765548481372022 1101 9 0.2814825784097579 1101 10-11 0.31832961327623366 1101 12-13 0.2905136380464981 1101 14-15 0.3498733970090271 1101 16-17 0.32215013608839627 1101 18-19 0.32396762928707545 1101 20-21 0.4016601114658229 1101 22-23 0.33655948472878094 1101 24-25 0.32267703439936923 1101 26-27 0.34593272262625874 1101 28-29 0.2954357630461004 1101 30-31 0.3774455505486287 1101 32-33 0.3974844853354469 1101 34-35 0.46063309206601843 1101 36-37 0.4431541488825772 1101 38-39 0.36364992801360785 1101 40-41 0.34664734091231963 1101 42-43 0.36186184167529944 1101 44-45 0.4978116767412999 1101 46-47 0.37948276338717335 1101 48-49 0.42974956983371726 1101 50-51 0.48586818100384477 1101 52-53 0.39434653987765245 1101 54-55 0.3445784729056456 1101 56-57 0.16801855156482048 1101 58-59 0.3198703797378073 1101 60-61 0.36813572270541073 1101 62-63 0.29751132365316835 1101 64-65 0.20915637565484957 1101 66-67 0.17665311207768042 1101 68-69 0.04437407600104848 1101 70-71 0.21832532865219179 1101 72-73 0.18717983880367228 1101 74-75 0.13597853920232694 1101 76-77 0.13761669918891428 1101 78-79 0.3450490438827458 1101 80-81 0.3365539303916556 1101 82-83 0.3336679356928549 1101 84-85 0.3246656077195702 1101 86-87 0.48209406917550623 1101 88-89 0.6032258912556756 1101 90-91 0.576614647460449 1101 92-93 0.3962470673336931 1101 94-95 0.27518789846880054 1101 96-97 0.30025703826180106 1101 98-99 0.26206453012969533 1101 100-101 -0.05645960174279807 1102 1 0.12329129691759988 1102 2 0.11512039644946981 1102 3 0.13601465462003404 1102 4 0.12989139165082264 1102 5 0.09708760344294376 1102 6 0.11420136895346644 1102 7 0.12549187350628443 1102 8 0.08626186798605318 1102 9 0.23827621548336708 1102 10-11 0.1797739914767149 1102 12-13 0.17338784076893177 1102 14-15 0.1446720659322196 1102 16-17 0.18179398965056492 1102 18-19 0.26917230293484806 1102 20-21 0.32024965032598374 1102 22-23 0.2848266246610507 1102 24-25 0.2835660129463591 1102 26-27 0.14960823580260296 1102 28-29 0.13181328117677538 1102 30-31 0.2268931785862307 1102 32-33 0.16989830946154427 1102 34-35 0.29241111490415506 1102 36-37 0.41521599959421707 1102 38-39 0.411397654563622 1102 40-41 0.3929948900915363 1102 42-43 0.30725509722523014 1102 44-45 0.4849097334480419 1102 46-47 0.3211266993025461 1102 48-49 0.32495149260788025 1102 50-51 0.45114644657630265 1102 52-53 0.40806838191799955 1102 54-55 0.29067553687573877 1102 56-57 0.369189247252109 1102 58-59 0.4995640189221504 1102 60-61 0.47205230929686337 1102 62-63 0.4004225437919118 1102 64-65 0.4156718976755158 1102 66-67 0.45679343085375734 1102 68-69 0.482551046102337 1102 70-71 0.48724640833721367 1102 72-73 0.33952529593484826 1102 74-75 0.49240577375081074 1102 76-77 0.49626297178976486 1102 78-79 0.5057652883277974 1102 80-81 0.35151816384428614 1102 82-83 0.33467485924382956 1102 84-85 0.4135518706871686 1102 86-87 0.43923788035000655 1102 88-89 0.5269396675269533 1102 90-91 0.8187375468051208 1102 92-93 0.6929274030163981 1102 94-95 0.5531389370706528 1102 96-97 0.4279608961630892 1102 98-99 0.39138015775621326 1102 100-101 -0.2414802471358115 1103 1 0.20190974556416563 1103 2 0.15831264012383883 1103 3 0.15386903808309427 1103 4 0.11287144895792522 1103 5 0.1700106627058986 1103 6 0.1602677975028186 1103 7 0.1777305147364885 1103 8 0.22255386044518133 1103 9 0.2789209865419551 1103 10-11 0.3028363449528939 1103 12-13 0.2537016611389973 1103 14-15 0.36031760962115555 1103 16-17 0.2755096351942612 1103 18-19 0.3357156430820467 1103 20-21 0.3223710724956206 1103 22-23 0.30405187510365295 1103 24-25 0.2561265982028047 1103 26-27 0.2999621004995703 1103 28-29 0.43792942296990134 1103 30-31 0.29729304790132716 1103 32-33 0.2646007143286724 1103 34-35 0.3841510342856935 1103 36-37 0.41341466713800656 1103 38-39 0.4122387178795748 1103 40-41 0.5036510077785934 1103 42-43 0.40795311355201136 1103 44-45 0.2161359198771251 1103 46-47 0.42332582600950275 1103 48-49 0.44709331221855564 1103 50-51 0.412492854286711 1103 52-53 0.49688996611924807 1103 54-55 0.559202108736983 1103 56-57 0.5592850092643076 1103 58-59 0.5410564758424883 1103 60-61 0.4926815394479931 1103 62-63 0.45957512283199264 1103 64-65 0.43349316840959773 1103 66-67 0.40805149380326355 1103 68-69 0.230598498850469 1103 70-71 0.3058637090758438 1103 72-73 0.16445167530076077 1103 74-75 0.10560053399257185 1103 76-77 0.22974965385017043 1103 78-79 0.29332284928833374 1103 80-81 0.2537980856099473 1103 82-83 0.47400660022281826 1103 84-85 0.3655317911283227 1103 86-87 0.5417151539803555 1103 88-89 0.5218403072648741 1103 90-91 0.5288731323926932 1103 92-93 0.4765335116526366 1103 94-95 0.3583014322693039 1103 96-97 0.3479097701095526 1103 98-99 0.5707385655196049 1103 100-101 0.41521139388688866 1104 1 0.01725117779255214 1104 2 0.04643357630354217 1104 3 0.08316213111232429 1104 4 -0.13215211374324554 1104 5 0.005284428354926263 1104 6 0.07202210998287484 1104 7 0.031234574990612884 1104 8 -0.021931533792397317 1104 9 0.10888374269069345 1104 10-11 0.05309797263368665 1104 12-13 0.10918186950745223 1104 14-15 0.04710486060476882 1104 16-17 0.14396375600853872 1104 18-19 0.11539790323397625 1104 20-21 0.1589139107053157 1104 22-23 0.18481319544486752 1104 24-25 0.1387992270844265 1104 26-27 0.028982770058952667 1104 28-29 0.01956033925682732 1104 30-31 0.08447189749166029 1104 32-33 0.04215980263239061 1104 34-35 -0.02310535695110616 1104 36-37 -0.03268143937525991 1104 38-39 0.029742128441164084 1104 40-41 0.19333075215505602 1104 42-43 -0.06691150141762847 1104 44-45 -0.08534086625861192 1104 46-47 0.019748756395145506 1104 48-49 -0.19753816557907555 1104 50-51 -0.2989699070491909 1104 52-53 -0.31734611347262387 1104 54-55 -0.10780501972577383 1104 56-57 -0.09018852581520065 1104 58-59 -0.22062015882800523 1104 60-61 -0.205539035704966 1104 62-63 -0.3476746547682694 1104 64-65 -0.04471635807995966 1104 66-67 -0.004039173966539522 1104 68-69 -0.15636975180375856 1104 70-71 -0.16264289937353027 1104 72-73 -0.3204196311129053 1104 74-75 -0.20612576779155134 1104 76-77 -0.23487111893380686 1104 78-79 -0.09419054008368377 1104 80-81 -0.11620991559347615 1104 82-83 0.07378254707511545 1104 84-85 0.020945279575204978 1104 86-87 -0.04776540322501077 1104 88-89 -0.1865856926261955 1104 90-91 -0.1260973396653391 1104 92-93 -0.035745158461761406 1104 94-95 -0.16590852994218963 1104 96-97 -0.01731290843918387 1104 98-99 -0.15539317682349107 1104 100-101 -0.7278762536555625 1105 1 0.12226029966972618 1105 2 0.19599589924271044 1105 3 0.1259348356871044 1105 4 0.10479915219531932 1105 5 0.0843728339299048 1105 6 0.09755780396243807 1105 7 0.1473642292492201 1105 8 0.16528448906915116 1105 9 0.15479101102220483 1105 10-11 0.1416623770418468 1105 12-13 0.14342320866824565 1105 14-15 0.17653910645542936 1105 16-17 0.25102715429095923 1105 18-19 0.13423742246902748 1105 20-21 0.15344109340490064 1105 22-23 0.23472889842270206 1105 24-25 0.28046735638270803 1105 26-27 0.23229340582165037 1105 28-29 0.28032705899023824 1105 30-31 0.28642104982454697 1105 32-33 0.19693568626789215 1105 34-35 0.2906223265865009 1105 36-37 0.31978919262991923 1105 38-39 0.24230504220110305 1105 40-41 0.2990357570744422 1105 42-43 0.3767967517799704 1105 44-45 0.2995183008571374 1105 46-47 0.10237837851705933 1105 48-49 0.046101858943700336 1105 50-51 0.2701533421189666 1105 52-53 0.1954390378912052 1105 54-55 0.30073302023249937 1105 56-57 0.28947778405174773 1105 58-59 0.16267976058305322 1105 60-61 0.05083360904611567 1105 62-63 0.12291223543795127 1105 64-65 0.1987212670677465 1105 66-67 0.14800910770912168 1105 68-69 0.12042143631455815 1105 70-71 0.15587531471619087 1105 72-73 0.05303657151636543 1105 74-75 0.035738086337524066 1105 76-77 0.0358276934917221 1105 78-79 0.2057962344304336 1105 80-81 0.019955113749798414 1105 82-83 0.06599341570525397 1105 84-85 0.11382187219562923 1105 86-87 0.14103026178208466 1105 88-89 0.3366321422505223 1105 90-91 0.12983311652740426 1105 92-93 0.19892563818217823 1105 94-95 -0.08487987142670761 1105 96-97 0.1788973718422362 1105 98-99 0.30637435374345046 1105 100-101 -0.09188303202608594 1106 1 0.11430409066306169 1106 2 0.054056111562324816 1106 3 0.11198903115882786 1106 4 0.007179409161409467 1106 5 0.0400311651255052 1106 6 0.06051887988574833 1106 7 0.055733193072889264 1106 8 0.028363283353506574 1106 9 0.13145647108061098 1106 10-11 0.13138023806309462 1106 12-13 0.14710523994092028 1106 14-15 0.08844673042258222 1106 16-17 0.07565641847121896 1106 18-19 0.0855746248480429 1106 20-21 0.10412379473024913 1106 22-23 0.06013337385321904 1106 24-25 0.15051194361900144 1106 26-27 0.1672780052741203 1106 28-29 0.13428134729772978 1106 30-31 0.11915492990348042 1106 32-33 0.10328797519981947 1106 34-35 -0.12713803864255624 1106 36-37 -0.044030303118411496 1106 38-39 0.11831212070627828 1106 40-41 -0.09670751262856925 1106 42-43 -0.12508306885207787 1106 44-45 0.023925813741286106 1106 46-47 0.05596656686880408 1106 48-49 -0.02880896362150054 1106 50-51 -0.04382039608180577 1106 52-53 0.012240208794921159 1106 54-55 0.0737868904531922 1106 56-57 0.13607926622485422 1106 58-59 0.06373531571072988 1106 60-61 0.07860243435669645 1106 62-63 -0.07708293615418427 1106 64-65 0.03569525942642571 1106 66-67 -0.10124856160951623 1106 68-69 -0.40419100249351203 1106 70-71 -0.28718190873008353 1106 72-73 -0.307458580228932 1106 74-75 -0.23378008595310718 1106 76-77 -0.17305545133622147 1106 78-79 -0.003440894900396785 1106 80-81 -0.21580226793791724 1106 82-83 -0.21153843253226157 1106 84-85 -0.2320940056829741 1106 86-87 -0.11761851272060397 1106 88-89 -0.02136053160326412 1106 90-91 0.07356868684530937 1106 92-93 0.12018082279620401 1106 94-95 -0.02093412614270207 1106 96-97 0.08555758285847403 1106 98-99 -0.016744329706721572 1106 100-101 -0.2807821965041697 1107 1 0.0038106213150257418 1107 2 0.008341406770917104 1107 3 9.810640596015219E-4 1107 4 0.009149393971881636 1107 5 0.0470767379485153 1107 6 0.04101478914910217 1107 7 0.09179034632636984 1107 8 0.053252958280623375 1107 9 0.14826029483692338 1107 10-11 0.04503769933824486 1107 12-13 -0.005266625719876572 1107 14-15 0.13602945130895705 1107 16-17 0.07232374800505426 1107 18-19 -0.0062003218757737955 1107 20-21 0.05069430334682323 1107 22-23 0.08703887015032308 1107 24-25 0.10578618022874053 1107 26-27 0.15898215214291866 1107 28-29 0.052215289058501924 1107 30-31 0.20839363401227473 1107 32-33 0.05028539847999269 1107 34-35 0.06061020158614383 1107 36-37 0.07453096484776012 1107 38-39 -0.07017415864851273 1107 40-41 -0.09154913673775411 1107 42-43 -0.1499508832662002 1107 44-45 0.03436653578697957 1107 46-47 0.09495796510952914 1107 48-49 0.022488493084111383 1107 50-51 -0.005931613909829991 1107 52-53 0.030794989124970584 1107 54-55 -0.002378098595400502 1107 56-57 -0.07384597082067756 1107 58-59 0.04181260602257453 1107 60-61 -0.01167626602875771 1107 62-63 0.02591237723390094 1107 64-65 0.029607318108496372 1107 66-67 0.03548288269342237 1107 68-69 0.06859096639121987 1107 70-71 0.04906028318828959 1107 72-73 -0.03640570740494553 1107 74-75 0.05190861187959683 1107 76-77 0.1211790146537517 1107 78-79 0.06233259351564868 1107 80-81 0.055424501741129006 1107 82-83 0.02104120672696652 1107 84-85 0.1804528004960737 1107 86-87 0.14117625373154752 1107 88-89 0.1492091533655895 1107 90-91 0.15454482221688792 1107 92-93 0.08367447125941041 1107 94-95 0.2186637250393879 1107 96-97 0.35028077940140534 1107 98-99 0.5677249746071347 1107 100-101 0.029301282448390253 1108 1 -0.03396216855998091 1108 2 -0.012105068526246043 1108 3 0.05576750999149738 1108 4 0.06590735532054026 1108 5 0.06420924707330045 1108 6 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-0.8061094091410652 2316 90-91 -0.8752589851234198 2316 92-93 -0.9811076308584958 2316 94-95 -0.6552380157632989 2316 96-97 -0.9000286687910766 2316 98-99 -0.8626087784231409 2316 100-101 -0.5814304309800384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 10.0 5 20.0 6 67.0 7 164.0 8 415.0 9 901.0 10 1630.0 11 2518.0 12 3872.0 13 5595.0 14 6830.0 15 9587.0 16 12758.0 17 18557.0 18 17833.0 19 12326.0 20 10591.0 21 10353.0 22 10849.0 23 11537.0 24 13078.0 25 15336.0 26 17774.0 27 21233.0 28 25973.0 29 31687.0 30 38916.0 31 47761.0 32 59748.0 33 78353.0 34 109253.0 35 163890.0 36 275822.0 37 469228.0 38 426101.0 39 45409.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.924165415358864 19.58438469425011 15.529030256343331 21.96241963404769 2 15.589210832892203 21.32656066705061 39.01346267324298 24.07076582681421 3 19.391775836014702 24.793950552181144 30.658629481442645 25.15564413036151 4 12.972891453464658 17.70709449782437 37.48035154166384 31.83966250704713 5 13.374615760669883 37.97327101157804 34.130472848436874 14.521640379315196 6 30.703417338821914 37.25884142669316 17.51544295443987 14.522298280045062 7 28.073231436318753 33.61796817987208 20.498061217156838 17.81073916665233 8 26.74771328888623 33.85380028765444 19.73762918892986 19.660857234529463 9 25.95226069873106 17.581384850671718 20.626200036235147 35.840154414362075 10-11 24.579803864610103 26.85879729673651 27.27292050231227 21.28847833634112 12-13 26.611679661049543 24.153762534274097 27.465609504540023 21.768948300136337 14-15 22.931656178120562 25.692863993036312 26.533592447234643 24.84188738160848 16-17 21.60948102113037 28.89816151491879 27.37016039140716 22.122197072543678 18-19 21.641042691948282 27.402078410147055 29.7128034852553 21.24407541264936 20-21 22.559416027069073 25.935281799125697 29.860317553499982 21.644984620305248 22-23 22.038409256764485 25.938520695026572 30.053335506092665 21.969734542116274 24-25 22.282187054175335 26.29747471245811 29.90516570823589 21.51517252513066 26-27 21.750110263110685 26.258612605395214 30.155309671700604 21.8359674597935 28-29 21.61391146275624 27.24389695857553 29.73946625019864 21.40272532846959 30-31 21.82218594091455 26.432827678071664 29.48804049560761 22.256945885406175 32-33 21.526992654791393 26.52638030103513 29.554925095471518 22.39170194870196 34-35 22.670497724571266 26.47861112172576 29.125589039071375 21.725302114631603 36-37 22.70997631424552 25.846301271255296 29.585552449088787 21.858169965410397 38-39 22.020049909646332 26.46370414614516 29.498448549026357 22.01779739518215 40-41 22.354472114230703 26.463073480516737 29.493713940379234 21.68874046487333 42-43 21.88665675331235 26.650001568995833 28.838016870248097 22.62532480744372 44-45 22.199107913064665 26.508094802013332 29.06889090369414 22.223906381227863 46-47 22.938148895091235 27.009218327340452 28.1616231138867 21.89100966368161 48-49 22.45947817074331 27.07199292297664 28.080205589957753 22.388323316322296 50-51 22.31327687697323 27.29528454354686 28.417689344606384 21.973749234873523 52-53 22.891706503399323 27.130738033038764 27.9455480869765 22.032007376585412 54-55 22.129553811725007 27.101841008673155 28.23444241900989 22.53416276059195 56-57 22.583125757218436 27.351817982147608 27.726694878799503 22.338361381834453 58-59 22.672177061412967 26.831264910911575 27.790953195047123 22.70560483262834 60-61 21.981122430387128 27.153398347187753 28.575390832196234 22.29008839022889 62-63 22.149389063520893 27.145514881565425 28.55707345661549 22.148022598298194 64-65 21.82307153714513 27.480287285251542 28.169526942924282 22.527114234679043 66-67 21.756983577386244 27.286175665967495 27.98345162099262 22.973389135653637 68-69 22.234191339369513 26.98679120621625 28.202074124008657 22.57694333040558 70-71 22.343554329835577 27.027327938039907 27.79254259212447 22.836575140000047 72-73 22.1292501652343 26.891338078990596 28.105392660459454 22.874019095315646 74-75 21.95300868125317 27.991677049689724 27.441596127899953 22.613718141157158 76-77 22.137590025431923 27.143411042409614 27.837165703597517 22.881833228560943 78-79 22.125760700413718 27.371980920811247 27.894153519148773 22.60810485962626 80-81 22.58786435651331 27.37288800378559 27.50854667331338 22.530700966387723 82-83 22.35764653369066 27.33866725237042 27.78480245529981 22.51888375863911 84-85 22.10700805979002 27.592407218284375 27.615585567075 22.6849991548506 86-87 22.48283419552881 27.593641217587876 27.272084777010463 22.65143980987285 88-89 22.182269977463342 27.599579247981993 27.040405913903363 23.177744860651302 90-91 22.042800686534434 27.257454477462545 27.446236204021453 23.25350863198157 92-93 22.31176701002337 27.20090567626628 27.656451323949305 22.83087598976104 94-95 21.891292532016994 26.976333792514307 27.79731799176308 23.335055683705622 96-97 21.876135511355873 26.646345968738583 28.015234956593734 23.46228356331181 98-99 21.78589494018195 27.164243233825946 27.288132977015366 23.76172884897673 100-101 21.853980207139294 26.22645137945257 28.181900995209936 23.737667418198196 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 456.0 1 311.5 2 199.0 3 236.0 4 213.5 5 217.0 6 390.5 7 902.5 8 1504.5 9 1790.5 10 1635.0 11 1386.5 12 1472.5 13 1636.0 14 1741.0 15 1939.0 16 2164.0 17 2404.0 18 2636.5 19 2977.5 20 3446.0 21 4073.0 22 5059.5 23 6121.0 24 7528.0 25 9575.5 26 12568.0 27 16181.0 28 19039.5 29 22018.0 30 25732.5 31 30664.0 32 37966.0 33 41066.5 34 40821.5 35 43140.0 36 47059.0 37 50728.5 38 53613.5 39 55285.0 40 56629.0 41 59625.0 42 60492.0 43 60499.5 44 62148.0 45 66024.5 46 68637.5 47 74911.5 48 89964.5 49 87285.0 50 72083.0 51 65769.0 52 63048.5 53 63959.0 54 65457.5 55 61839.5 56 56379.5 57 50555.0 58 46397.5 59 41447.0 60 32793.0 61 27516.0 62 23655.5 63 16870.0 64 10718.5 65 7878.0 66 6144.0 67 5126.5 68 4016.0 69 2931.5 70 2224.0 71 1502.5 72 1046.5 73 849.0 74 635.5 75 436.0 76 304.5 77 193.0 78 129.5 79 79.0 80 46.0 81 26.5 82 14.5 83 11.0 84 8.0 85 3.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3395273843587644 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.603317236695476E-4 16-17 0.0017459673215646703 18-19 2.530387422557493E-5 20-21 0.0 22-23 0.0 24-25 4.5546973606034875E-4 26-27 0.0013411053339554713 28-29 0.0 30-31 0.003238895900873591 32-33 0.0 34-35 0.010146853564455547 36-37 0.026973929924462875 38-39 0.02085039236187374 40-41 0.013208622345750112 42-43 0.009919118696425372 44-45 0.0027075145421365174 46-47 0.0013664092081810463 48-49 5.060774845114986E-4 50-51 7.59116226767248E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.004048619876091989 60-61 0.010020334193327672 62-63 0.004099227624543139 64-65 0.0018471828184669697 66-67 0.003972708253415264 68-69 0.003213592026648016 70-71 2.530387422557493E-5 72-73 0.0 74-75 0.0 76-77 0.214956411546259 78-79 3.54254239158049E-4 80-81 0.003390719146227041 82-83 5.819891071882233E-4 84-85 0.0 86-87 0.10855362042771644 88-89 0.40875878423993744 90-91 0.3965623168632103 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0015941440762112205 100-101 8.603317236695476E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1975982.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.57705671803749 #Duplication Level Percentage of deduplicated Percentage of total 1 71.65331313769431 34.09053743187383 2 15.015523121476098 14.287887904029434 3 5.586852567160599 7.974180043893396 4 2.5777260341544608 4.905624709221145 5 1.4430599187109676 3.4328271800019143 6 0.8316381632935577 2.3740137638341268 7 0.5741378680956682 1.9121052940052514 8 0.39599162800501886 1.507209291637023 9 0.28269705270129686 1.2104904339352474 >10 1.4625903343205502 12.714356397835816 >50 0.10697466326597972 3.488277610595013 >100 0.058535606699312785 5.0970195532835705 >500 0.005852376147808427 1.9574249883945862 >1k 0.004788307757297804 4.01721421645124 >5k 3.192205171531869E-4 1.03083118100842 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7207 0.36473004308743706 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7006 0.3545578856487559 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6149 0.31118704522612045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3919 0.19833176618005627 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3592 0.1817830324365303 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2886 0.1460539620300185 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2871 0.14529484580325125 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2475 0.1252541774165959 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2462 0.12459627668673094 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2414 0.12216710476107577 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2321 0.11746058415511881 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2247 0.11371561076973372 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2242 0.11346257202747799 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2233 0.11300710229141762 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2124 0.1074908577102423 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2117 0.10713660347108425 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2021 0.10227825961977385 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2004 0.1014179278961043 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.060774845114986E-5 0.0 12-13 0.0 0.0 0.0 5.060774845114986E-5 2.0243099380459943E-4 14-15 0.0 0.0 0.0 5.060774845114986E-5 2.530387422557493E-4 16-17 0.0 0.0 0.0 1.5182324535344957E-4 3.54254239158049E-4 18-19 0.0 0.0 0.0 1.7712711957902448E-4 3.54254239158049E-4 20-21 0.0 2.530387422557493E-5 0.0 2.783426164813242E-4 4.0486198760919886E-4 22-23 0.0 5.060774845114986E-5 0.0 5.566852329626484E-4 4.0486198760919886E-4 24-25 0.0 5.060774845114986E-5 0.0 5.819891071882233E-4 4.0486198760919886E-4 26-27 0.0 5.060774845114986E-5 0.0 0.0011892820886020218 4.5546973606034875E-4 28-29 0.0 5.060774845114986E-5 0.0 0.002935249410166692 5.060774845114986E-4 30-31 0.0 5.060774845114986E-5 0.0 0.008274366871763002 5.313813587370735E-4 32-33 0.0 5.060774845114986E-5 0.0 0.018345308813541822 6.072929814137983E-4 34-35 0.0 5.060774845114986E-5 0.0 0.033299898480856606 6.579007298649482E-4 36-37 0.0 5.060774845114986E-5 0.0 0.056225208529227494 6.579007298649482E-4 38-39 0.0 5.060774845114986E-5 0.0 0.0932447765212436 6.579007298649482E-4 40-41 0.0 5.060774845114986E-5 0.0 0.13578058909443508 6.579007298649482E-4 42-43 0.0 5.060774845114986E-5 0.0 0.17839231329030325 6.579007298649482E-4 44-45 0.0 5.060774845114986E-5 0.0 0.23686956662560693 7.08508478316098E-4 46-47 0.0 5.060774845114986E-5 0.0 0.30744207184073535 7.844201009928228E-4 48-49 0.0 5.060774845114986E-5 0.0 0.3833283906432346 8.097239752183977E-4 50-51 0.0 5.060774845114986E-5 0.0 0.47014598311118216 9.109394721206975E-4 52-53 0.0 5.060774845114986E-5 0.0 0.5557489896163021 9.615472205718473E-4 54-55 0.0 5.060774845114986E-5 0.0 0.6532195131332168 9.615472205718473E-4 56-57 0.0 5.060774845114986E-5 0.0 0.771312694143975 9.615472205718473E-4 58-59 0.0 5.060774845114986E-5 0.0 0.9031205749849949 0.0010121549690229972 60-61 0.0 5.060774845114986E-5 0.0 1.0509964159592546 0.001062762717474147 62-63 0.0 5.060774845114986E-5 0.0 1.2009218707457863 0.001062762717474147 64-65 0.0 5.060774845114986E-5 0.0 1.3595518582659154 0.001062762717474147 66-67 0.0 5.060774845114986E-5 0.0 1.5526457224812775 0.001062762717474147 68-69 0.0 5.060774845114986E-5 0.0 1.7738521909612537 0.001062762717474147 70-71 0.0 5.060774845114986E-5 0.0 2.014795681337178 0.001062762717474147 72-73 0.0 5.060774845114986E-5 0.0 2.270314203267034 0.0011133704659252969 74-75 0.0 5.060774845114986E-5 0.0 2.53663747949121 0.0011892820886020216 76-77 0.0 5.060774845114986E-5 0.0 2.8262150161286894 0.0012145859628275965 78-79 0.0 5.060774845114986E-5 0.0 3.1747758835859843 0.0012651937112787464 80-81 0.0 5.060774845114986E-5 0.0 3.5564848262787816 0.0013158014597298962 82-83 0.0 5.060774845114986E-5 0.0 3.9451523343836126 0.0015435363277600707 84-85 0.0 5.060774845114986E-5 0.0 4.356112555681175 0.0015688402019856456 86-87 0.0 5.060774845114986E-5 0.0 4.784254107577903 0.0015941440762112205 88-89 0.0 5.060774845114986E-5 0.0 5.245341303716329 0.0016194479504367954 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1350 0.0 24.601803 5 GTGTAAG 955 0.0 24.43384 1 TAAGGTA 780 0.0 22.506592 4 TATTCCG 170 3.1819563E-6 22.327684 5 GAGTACT 8340 0.0 21.903791 12-13 GTAAGGT 1500 0.0 21.509 4 GGTGTGC 1855 0.0 20.206255 8 TACATGG 8710 0.0 20.155157 2 GTACTTT 8990 0.0 20.056192 14-15 GTACATG 8990 0.0 19.970121 1 AGTACTT 8925 0.0 19.909859 12-13 ACATGGG 8525 0.0 19.479431 3 GTATAGG 640 0.0 19.346054 1 AGGTAAG 1585 0.0 19.15814 2 GGTAAGG 1600 0.0 18.978533 3 AAGGTAA 1510 0.0 18.606903 1 GTATCAA 20740 0.0 18.162128 1 AGAGTAC 15390 0.0 18.02065 10-11 ACCGTGC 265 4.0574378E-7 17.904276 8 ACTTTTT 10120 0.0 17.816042 16-17 >>END_MODULE