##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939110_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943079 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.203799742573516 33.0 31.0 34.0 31.0 34.0 2 32.30108811839354 34.0 31.0 34.0 31.0 34.0 3 32.361309550460895 34.0 31.0 34.0 30.0 34.0 4 35.795643923896044 37.0 35.0 37.0 35.0 37.0 5 35.843945099504445 37.0 35.0 37.0 35.0 37.0 6 35.930268403909466 37.0 37.0 37.0 35.0 37.0 7 35.87362788646267 37.0 37.0 37.0 35.0 37.0 8 35.862228452883286 37.0 37.0 37.0 35.0 37.0 9 37.55699176410223 39.0 38.0 39.0 35.0 39.0 10-11 37.472360362085126 39.0 38.0 39.0 35.0 39.0 12-13 37.3910026303614 39.0 38.0 39.0 35.0 39.0 14-15 38.69179225342871 40.0 38.0 41.0 35.0 41.0 16-17 38.61488621924276 40.0 38.0 41.0 34.0 41.0 18-19 38.54081640530313 40.0 38.0 41.0 34.0 41.0 20-21 38.52234958022808 40.0 38.0 41.0 34.0 41.0 22-23 38.57103056540676 40.0 38.0 41.0 34.0 41.0 24-25 38.51638662143948 40.0 38.0 41.0 34.0 41.0 26-27 38.39289344385895 40.0 38.0 41.0 34.0 41.0 28-29 38.257189491523505 40.0 38.0 41.0 34.0 41.0 30-31 38.03691126300063 40.0 38.0 41.0 33.5 41.0 32-33 37.8136722696298 40.0 38.0 41.0 33.0 41.0 34-35 37.50435571585098 40.0 37.5 41.0 32.5 41.0 36-37 37.22777071853486 40.0 37.0 41.0 31.5 41.0 38-39 37.01955942089848 40.0 37.0 41.0 31.0 41.0 40-41 36.829178329856894 40.0 36.5 41.0 30.5 41.0 42-43 36.608152061753536 40.0 36.0 41.0 30.0 41.0 44-45 36.32361036272843 40.0 36.0 41.0 30.0 41.0 46-47 36.14545677247297 40.0 36.0 41.0 29.0 41.0 48-49 35.97889432184692 40.0 36.0 41.0 28.0 41.0 50-51 34.827969938432766 38.5 34.0 40.0 25.5 40.5 52-53 34.94381468792571 38.5 34.5 39.5 26.5 40.5 54-55 35.45629693903336 39.0 35.0 41.0 27.0 41.0 56-57 35.35196870533828 39.0 35.0 41.0 26.0 41.0 58-59 35.13167092022506 39.0 35.0 41.0 26.0 41.0 60-61 34.81432973131818 38.5 35.0 40.5 26.0 41.0 62-63 34.440320234020334 38.0 34.5 40.0 25.0 41.0 64-65 34.05898190449282 37.0 34.0 40.0 23.5 41.0 66-67 33.67005278735451 37.0 34.0 39.5 22.5 41.0 68-69 33.26167129591745 36.0 34.0 39.0 22.0 41.0 70-71 32.81101669052056 35.5 33.0 39.0 20.5 40.5 72-73 32.38121378492588 35.0 33.0 38.0 18.5 40.0 74-75 31.956146662076016 35.0 32.5 37.0 18.0 39.5 76-77 31.52576735171344 35.0 32.0 37.0 14.5 39.0 78-79 31.102827522710093 35.0 31.5 36.0 9.5 39.0 80-81 30.67657722614469 35.0 31.0 36.0 7.0 37.5 82-83 30.283758148793744 35.0 31.0 35.0 2.0 37.0 84-85 29.90682365462238 34.5 31.0 35.0 2.0 37.0 86-87 29.53978659642763 34.0 30.0 35.0 2.0 36.0 88-89 29.116268304067923 34.0 30.0 35.0 2.0 36.0 90-91 28.709333742992435 34.0 29.0 35.0 2.0 35.5 92-93 28.32610974643851 34.0 29.0 35.0 2.0 35.0 94-95 27.86018144398658 34.0 29.0 35.0 2.0 35.0 96-97 27.2940469739007 34.0 27.0 35.0 2.0 35.0 98-99 26.610407760055047 34.0 25.5 35.0 2.0 35.0 100-101 23.67103859390174 30.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09161980801583525 1101 2 0.2239129076540678 1101 3 0.2200143880154073 1101 4 0.230583394171191 1101 5 0.1937015665400068 1101 6 0.14339373978088332 1101 7 0.2713007421756899 1101 8 0.1507701200442213 1101 9 0.271367751275001 1101 10-11 0.2017286695083058 1101 12-13 0.1661899677642964 1101 14-15 0.25426267458184526 1101 16-17 0.3186733549481673 1101 18-19 0.3807860391438709 1101 20-21 0.36505404826149856 1101 22-23 0.38819737942736054 1101 24-25 0.4798343155520328 1101 26-27 0.45751372005080526 1101 28-29 0.40042958648975 1101 30-31 0.445009607400344 1101 32-33 0.3359743205713812 1101 34-35 0.384927880407254 1101 36-37 0.4082098171616124 1101 38-39 0.3758557458826033 1101 40-41 0.35344089487265506 1101 42-43 0.2682658595887375 1101 44-45 0.18739256131227222 1101 46-47 0.43645818376912615 1101 48-49 0.5520448706728232 1101 50-51 0.5234402152303375 1101 52-53 0.4363308842636613 1101 54-55 0.533557275345423 1101 56-57 0.596587736067498 1101 58-59 0.5279965056852234 1101 60-61 0.45269846229786026 1101 62-63 0.3013770662163111 1101 64-65 0.3662593291824834 1101 66-67 0.28128332818246093 1101 68-69 0.37985532905042163 1101 70-71 0.38510026796458163 1101 72-73 0.4153349089092231 1101 74-75 0.3099708189900916 1101 76-77 0.09242770017689494 1101 78-79 0.16658017933383107 1101 80-81 0.09253538365626923 1101 82-83 -0.07207116578572581 1101 84-85 -0.14921561889560664 1101 86-87 -0.11457618150765114 1101 88-89 -0.07361750552985313 1101 90-91 0.005910407656845251 1101 92-93 -0.16739830077968065 1101 94-95 -0.22847663881258384 1101 96-97 -0.14292175523593897 1101 98-99 -0.21880906134150635 1101 100-101 -0.19296170997978024 1102 1 0.14551683183142217 1102 2 0.2419409470647409 1102 3 0.25215385998691175 1102 4 0.18348401282035098 1102 5 0.24990960679515695 1102 6 0.23542890479524914 1102 7 0.2732543136406633 1102 8 0.2179300996021496 1102 9 0.33792495193485195 1102 10-11 0.36697380687346737 1102 12-13 0.28625471553627335 1102 14-15 0.4276618889308921 1102 16-17 0.4355522196854551 1102 18-19 0.43430741813892126 1102 20-21 0.4236302524725062 1102 22-23 0.4886643510749096 1102 24-25 0.4402794983440117 1102 26-27 0.473042920611654 1102 28-29 0.41779933747218223 1102 30-31 0.42035136410771656 1102 32-33 0.5227205739510978 1102 34-35 0.36989608163639076 1102 36-37 0.315192406460973 1102 38-39 0.1758470827559293 1102 40-41 0.4035146770481717 1102 42-43 0.3103257035942235 1102 44-45 0.2782347264241949 1102 46-47 0.4970343690522938 1102 48-49 0.45538676278705026 1102 50-51 0.3468687677310669 1102 52-53 0.25242344285624796 1102 54-55 0.2905227203898946 1102 56-57 0.2465573787244395 1102 58-59 0.3142797185841104 1102 60-61 0.18086265111616484 1102 62-63 0.19685917285576693 1102 64-65 0.28120580707550147 1102 66-67 0.3178535526520818 1102 68-69 0.3112055101024893 1102 70-71 0.2441519249513533 1102 72-73 0.3224711049084661 1102 74-75 0.2934005872405301 1102 76-77 0.2591133553495091 1102 78-79 0.19401062316255846 1102 80-81 0.32801955051827747 1102 82-83 0.23991077559912455 1102 84-85 0.2406059716544533 1102 86-87 0.15127188035435424 1102 88-89 0.15476107747382173 1102 90-91 0.25365289876440045 1102 92-93 0.3107385882696896 1102 94-95 0.052865133910650286 1102 96-97 -0.16662286862971598 1102 98-99 -0.058948348402580564 1102 100-101 -0.30128027717142913 1103 1 0.20710091279809006 1103 2 0.2938893883633895 1103 3 0.30689423149765105 1103 4 0.24791378123349972 1103 5 0.31837357997167715 1103 6 0.32907056113834443 1103 7 0.31954963038621287 1103 8 0.3538383814899291 1103 9 0.3574667404311498 1103 10-11 0.4415123475368503 1103 12-13 0.4042776898620133 1103 14-15 0.5485710409277473 1103 16-17 0.49267014272646037 1103 18-19 0.5872493344809513 1103 20-21 0.5663219392263485 1103 22-23 0.5665053433783171 1103 24-25 0.5965309305780124 1103 26-27 0.6599221925457286 1103 28-29 0.5598142344288632 1103 30-31 0.5744902404092258 1103 32-33 0.546914086211018 1103 34-35 0.3731642458096118 1103 36-37 0.2897164740227893 1103 38-39 0.31023241530127876 1103 40-41 0.17846621800273255 1103 42-43 0.11095580040893083 1103 44-45 0.153506570900376 1103 46-47 0.30598942834893705 1103 48-49 0.3326884597582094 1103 50-51 0.2604100772366209 1103 52-53 0.35370827521810355 1103 54-55 0.19091694010880644 1103 56-57 0.17617382430299955 1103 58-59 0.13134505602275226 1103 60-61 0.03270082116476658 1103 62-63 0.0536493835255385 1103 64-65 0.09302188841432724 1103 66-67 0.09255784478818896 1103 68-69 -0.007293008989080363 1103 70-71 -0.048576719901127774 1103 72-73 0.010165694897551703 1103 74-75 0.03212578267048016 1103 76-77 -0.04740367552816238 1103 78-79 -0.08750343556033258 1103 80-81 0.01177194331825504 1103 82-83 -0.0828909067266359 1103 84-85 -0.15784196050115895 1103 86-87 -0.16280252172563436 1103 88-89 -0.16860040374474394 1103 90-91 -0.11640759535418255 1103 92-93 -0.2774179348777963 1103 94-95 -0.29812825498330753 1103 96-97 -0.2652499915666553 1103 98-99 -0.46241948059541116 1103 100-101 -0.5198406515423173 1104 1 0.11770098714533361 1104 2 0.24328753757765043 1104 3 0.29199616288106256 1104 4 0.2359395988064179 1104 5 0.2721723621243868 1104 6 0.24457854030795545 1104 7 0.24413192061020794 1104 8 0.2526297453670381 1104 9 0.3091308356482543 1104 10-11 0.3004782629454752 1104 12-13 0.33564465929082843 1104 14-15 0.3894343900406838 1104 16-17 0.5167351274786895 1104 18-19 0.4511881263053823 1104 20-21 0.4815659198960631 1104 22-23 0.45170284057385146 1104 24-25 0.43087442141126786 1104 26-27 0.49387503139315214 1104 28-29 0.5067457658833376 1104 30-31 0.45932965365816614 1104 32-33 0.46003818359932325 1104 34-35 0.5618504708448953 1104 36-37 0.37932891435583826 1104 38-39 0.24367765329469648 1104 40-41 0.3237489513494509 1104 42-43 0.3800546526322819 1104 44-45 0.6116095977542031 1104 46-47 0.6951700954344986 1104 48-49 0.6745624049978503 1104 50-51 0.47616182324539835 1104 52-53 0.5367939488995077 1104 54-55 0.3878270885072155 1104 56-57 0.49743088482362907 1104 58-59 0.3976086794967415 1104 60-61 0.3625374217194093 1104 62-63 0.31772749418449564 1104 64-65 0.3487788650475778 1104 66-67 0.4153750459764325 1104 68-69 0.3123353205994732 1104 70-71 0.3718324202039369 1104 72-73 0.3561591681309011 1104 74-75 0.22759076544889822 1104 76-77 0.22753061269569486 1104 78-79 0.26612691390459986 1104 80-81 0.30169493141940507 1104 82-83 0.29659270891786704 1104 84-85 0.19647363366817316 1104 86-87 0.31139602488449114 1104 88-89 0.2118653521133922 1104 90-91 0.3394934701799528 1104 92-93 0.22002201985591796 1104 94-95 0.2584726944243805 1104 96-97 0.2545656864900536 1104 98-99 0.13827020520525224 1104 100-101 0.1009512452663408 1105 1 0.08878441984439434 1105 2 0.19229582308410187 1105 3 0.23299440913832825 1105 4 0.21964047137971932 1105 5 0.3024320762506463 1105 6 0.2865921730040242 1105 7 0.2505002274246664 1105 8 0.2714972951351058 1105 9 0.31504296095913276 1105 10-11 0.27338316554535425 1105 12-13 0.24055246894884874 1105 14-15 0.3263908225906178 1105 16-17 0.3049065462259648 1105 18-19 0.43274663899087074 1105 20-21 0.3385113402700881 1105 22-23 0.3304961314170711 1105 24-25 0.3684775181054647 1105 26-27 0.37973213489782864 1105 28-29 0.2890664370595104 1105 30-31 0.4171779617490188 1105 32-33 0.5699015701006473 1105 34-35 0.4817017250672606 1105 36-37 0.4413731805678651 1105 38-39 0.5096824280595555 1105 40-41 0.4819289922853116 1105 42-43 0.6452035579594764 1105 44-45 0.571491348735762 1105 46-47 0.34075044231477847 1105 48-49 0.6221005552518548 1105 50-51 0.5981654661851934 1105 52-53 0.494881581333793 1105 54-55 0.6009538822187466 1105 56-57 0.5427201677167233 1105 58-59 0.6736097227555149 1105 60-61 0.6195118540648679 1105 62-63 0.4993984404697045 1105 64-65 0.4219090581416296 1105 66-67 0.5244436151685221 1105 68-69 0.45994731950802503 1105 70-71 0.44058021011689164 1105 72-73 0.5752117628300795 1105 74-75 0.5363798949790457 1105 76-77 0.3827646849280164 1105 78-79 0.3687390984116199 1105 80-81 0.4228467750634408 1105 82-83 0.32501450977005675 1105 84-85 0.17181964457094168 1105 86-87 0.24058760353298325 1105 88-89 0.22383970956823163 1105 90-91 0.23607230426989645 1105 92-93 0.2811513181699681 1105 94-95 0.38701461330417075 1105 96-97 0.2936760055696439 1105 98-99 0.2860725239488069 1105 100-101 0.18207829034983547 1106 1 0.09454583312736986 1106 2 0.1780778640745453 1106 3 0.2345328740497692 1106 4 0.13410846225792739 1106 5 0.09894534848596237 1106 6 0.05964604777761906 1106 7 0.1474393650386645 1106 8 0.10354559892429194 1106 9 0.13793345213723285 1106 10-11 0.25569802278789666 1106 12-13 0.24458263939835945 1106 14-15 0.2621287486008512 1106 16-17 0.2111867216084562 1106 18-19 0.2707997166567324 1106 20-21 0.37620028534106353 1106 22-23 0.3272403613123416 1106 24-25 0.37793350225476274 1106 26-27 0.24887382369507094 1106 28-29 0.30652654173901084 1106 30-31 0.3160221711919746 1106 32-33 0.3066778033234101 1106 34-35 0.21125873048540456 1106 36-37 0.40025917787492915 1106 38-39 0.4386944247658846 1106 40-41 0.6172760066392016 1106 42-43 0.6184409397753328 1106 44-45 0.48407509594392906 1106 46-47 0.3717211316140876 1106 48-49 0.5044294440962034 1106 50-51 0.4851644041056957 1106 52-53 0.5220819040606344 1106 54-55 0.37513310398477273 1106 56-57 0.4139675363998734 1106 58-59 0.4729866319381557 1106 60-61 0.6665175634860532 1106 62-63 0.6254166320655798 1106 64-65 0.4137671272366177 1106 66-67 0.5745311090962488 1106 68-69 0.6222287452064634 1106 70-71 0.5006999861249213 1106 72-73 0.6557461100301651 1106 74-75 0.6925343966958799 1106 76-77 0.6060575824005419 1106 78-79 0.5897304060222304 1106 80-81 0.6909499687249152 1106 82-83 0.7904773506119156 1106 84-85 0.7900924232947766 1106 86-87 0.6871780965589913 1106 88-89 0.7494174717344606 1106 90-91 0.7599300989969606 1106 92-93 0.5445650440206578 1106 94-95 0.7321451933323786 1106 96-97 0.6221514187893504 1106 98-99 0.6497663180657476 1106 100-101 0.30195468070774467 1107 1 0.07715106785281023 1107 2 0.08591299000242714 1107 3 0.08076330652904318 1107 4 0.046232305911011906 1107 5 0.06689006858344726 1107 6 0.10790358771533448 1107 7 0.14007563761319375 1107 8 0.2298348418727798 1107 9 0.12336350369331939 1107 10-11 0.11661698786176089 1107 12-13 0.18487408077280776 1107 14-15 0.17644662621413687 1107 16-17 0.2052585742139641 1107 18-19 0.28499164972377855 1107 20-21 0.22609683089236654 1107 22-23 0.26844443115674466 1107 24-25 0.379808282843797 1107 26-27 0.3410017443409217 1107 28-29 0.43039957582431043 1107 30-31 0.30041086308006015 1107 32-33 0.2807502798966013 1107 34-35 0.25414257587105027 1107 36-37 0.2777984007564882 1107 38-39 0.2243291134923382 1107 40-41 0.2368065587930559 1107 42-43 0.39673461834655654 1107 44-45 0.3598135965374567 1107 46-47 0.38565439069728313 1107 48-49 0.2610182854336358 1107 50-51 0.05205676937637804 1107 52-53 0.15413815323334035 1107 54-55 0.13005328609852285 1107 56-57 0.1076742164586264 1107 58-59 0.1940921572932126 1107 60-61 0.02147794002328851 1107 62-63 0.08978444223886584 1107 64-65 0.00813709100711435 1107 66-67 -0.1010991361321345 1107 68-69 -0.08853618163029608 1107 70-71 -0.03797374329058556 1107 72-73 0.024298229354592138 1107 74-75 -0.1445061427272165 1107 76-77 -0.08049406622205169 1107 78-79 0.01637709764558437 1107 80-81 -0.03029716094798829 1107 82-83 -0.013539244505448522 1107 84-85 0.11579386444811135 1107 86-87 -0.08540294578811825 1107 88-89 -0.042464920654587957 1107 90-91 0.09496318897279821 1107 92-93 0.17949351339459696 1107 94-95 0.2720471698257576 1107 96-97 0.25175950347206566 1107 98-99 0.3127109366927563 1107 100-101 0.017511739978317564 1108 1 0.006266816877058545 1108 2 0.1253885107624697 1108 3 0.051074312206928596 1108 4 0.09411609449951897 1108 5 0.17467655481377875 1108 6 0.11188758509609897 1108 7 -0.01669603786563556 1108 8 0.12546111810463856 1108 9 0.1941489588862737 1108 10-11 0.19045247565157553 1108 12-13 0.2127754171006515 1108 14-15 0.270237282545537 1108 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Count 2 5357.0 3 1354.0 4 1366.0 5 1528.0 6 1751.0 7 2209.0 8 2781.0 9 3562.0 10 4401.0 11 5516.0 12 7443.0 13 12150.0 14 10715.0 15 11295.0 16 13755.0 17 19080.0 18 15781.0 19 11645.0 20 10929.0 21 10877.0 22 11716.0 23 12861.0 24 14552.0 25 16017.0 26 18003.0 27 20902.0 28 25079.0 29 29596.0 30 35455.0 31 43800.0 32 55406.0 33 72625.0 34 102691.0 35 156844.0 36 259735.0 37 423782.0 38 413668.0 39 76838.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.559007687221026 19.09458174904943 15.179730120681104 22.166680443048435 2 15.279674124849423 20.724511635527644 39.73298517259821 24.262829067024718 3 19.468682293961102 24.637488503659508 30.390527788297305 25.503301414082085 4 12.725815843308617 17.06229420342878 37.894961945347646 32.31692800791496 5 13.454018901565512 37.664255016541446 34.34565954508446 14.536066536808583 6 30.728021284726477 36.856702000438794 17.885031163830796 14.53024555100393 7 28.134870027257502 33.39430044234204 20.646010989531717 17.824818540868737 8 26.72027722423466 33.37326741291547 20.055646749244023 19.850808613605846 9 26.109865251434165 17.518549688855558 20.853270235719854 35.518314823990416 10-11 24.60605395728103 26.592355785746918 27.49528346345978 21.30630679351227 12-13 26.569937458231447 23.919515941905818 27.578924054952285 21.93162254491045 14-15 23.061188858009878 25.446609752228543 26.58871833327496 24.90348305648662 16-17 21.747973446600916 28.696248500589977 27.38691396824367 22.16886408456544 18-19 21.799898527620318 27.21666928503179 29.676550351161396 21.30688183618649 20-21 22.690593376673647 25.93498746945914 29.616656360830127 21.757762793037084 22-23 22.106589218167496 25.932995646612717 29.89328508942963 22.067130045790154 24-25 22.314506456518963 26.188469353688642 29.75233180695977 21.744692382832625 26-27 21.81050611954067 26.18188628326939 29.927852507619317 22.07975508957062 28-29 21.680022111248118 27.17725859161344 29.576705089167522 21.56601420797092 30-31 21.86837420335903 26.40435981282414 29.3250418796709 22.402224104145933 32-33 21.567320981250376 26.451757799722763 29.392773676041905 22.58814754298495 34-35 22.67624052909274 26.478778325581036 28.957138375619184 21.88784276970704 36-37 22.71260994791621 25.774957873023574 29.364895410759363 22.147536768300853 38-39 22.065706328548195 26.423618151797427 29.307037835556237 22.20363768409814 40-41 22.380881952161957 26.39544859605747 29.327652783502167 21.896016668278403 42-43 21.90972600298622 26.560865335946943 28.70028131901556 22.829127342051283 44-45 22.158263779173172 26.46976812520887 28.988978937686298 22.382989157931657 46-47 22.960457279071576 26.91315158426692 28.040183406174528 22.08620773048697 48-49 22.416009192098457 27.02571619913351 27.980751845158046 22.577522763609988 50-51 22.294940588281552 27.253599675522207 28.346177245994724 22.105282490201517 52-53 22.783500754656952 27.073987421090816 27.994061037326745 22.148450786925487 54-55 22.061108783255996 27.025052894041206 28.25547951228849 22.658358810414306 56-57 22.5252527342045 27.292915167775796 27.74496134775027 22.43687075026944 58-59 22.673798976021146 26.757614039297167 27.79850234948385 22.770084635197836 60-61 21.981443110346408 27.071744106776542 28.577243104867822 22.369569678009228 62-63 22.108014027392723 27.054557868167333 28.612389829105805 22.225038275334132 64-65 21.77954809488419 27.43437644135392 28.189345175533788 22.596730288228102 66-67 21.800228751431636 27.230397603723976 27.964694356059027 23.00467928878536 68-69 22.260022578439116 26.915098496694096 28.2707880899898 22.554090834876988 70-71 22.369344343904046 26.993095796726013 27.754462479158537 22.8830973802114 72-73 22.096113836963173 26.832758239773913 28.195649778111676 22.875478145151234 74-75 22.04491953595961 27.922831721586117 27.51160527849693 22.52064346395734 76-77 22.251049914555825 27.16366976207282 27.73028867380344 22.854991649567914 78-79 22.21101411110097 27.37756833751418 27.84317183396216 22.568245717422688 80-81 22.697232997155417 27.422498060512023 27.411274890095683 22.468994052236877 82-83 22.47576118805418 27.301384251024785 27.79478603349918 22.428068527421857 84-85 22.320805343091934 27.562259564095765 27.645871452863247 22.47106363994905 86-87 22.627097274755116 27.564038405586267 27.322354766753953 22.486509552904664 88-89 22.39426488811364 27.605954960945756 26.971418846340768 23.028361304599837 90-91 22.30189827277821 27.38810414345932 27.332923900935295 22.97707368282717 92-93 22.66526822761303 27.218310168883765 27.554716860073132 22.56170474343007 94-95 22.247275654789682 27.050799944949134 27.708538782403174 22.993385617858003 96-97 22.270240204892055 26.675678122857622 27.962281235043783 23.091800437206537 98-99 22.311190792179872 27.13505165040707 27.17214991423937 23.381607643173687 100-101 22.60258790745938 26.200927123576218 27.95280427336567 23.243680695598737 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1813.0 1 1017.0 2 262.0 3 304.5 4 271.5 5 293.0 6 504.0 7 1030.5 8 1655.0 9 1955.5 10 1830.0 11 1696.0 12 1825.5 13 2090.0 14 2319.5 15 2515.0 16 2714.0 17 2855.5 18 3037.0 19 3278.0 20 3708.5 21 4280.5 22 4940.5 23 5978.0 24 7413.0 25 9371.0 26 12393.0 27 15998.5 28 18678.0 29 21248.0 30 24658.5 31 29461.0 32 36547.0 33 39433.0 34 39177.5 35 41720.5 36 45815.0 37 49820.0 38 52652.5 39 53975.5 40 55201.5 41 58305.5 42 59179.0 43 59052.5 44 60401.5 45 64104.0 46 67129.0 47 72665.0 48 87591.0 49 85608.0 50 70253.5 51 63903.0 52 61594.0 53 62771.0 54 64423.5 55 61375.0 56 55723.5 57 49967.5 58 46256.0 59 41353.0 60 32729.5 61 27377.0 62 23553.5 63 16859.0 64 10820.0 65 8039.0 66 6381.5 67 5282.5 68 4169.0 69 3090.0 70 2370.0 71 1607.5 72 1078.0 73 869.5 74 639.0 75 475.5 76 382.5 77 260.0 78 169.0 79 119.5 80 87.0 81 58.5 82 45.0 83 34.0 84 21.5 85 16.0 86 13.0 87 11.0 88 7.5 89 7.5 90 7.0 91 2.0 92 1.0 93 2.0 94 1.0 95 0.0 96 2.0 97 2.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3807873997917738 2 0.11389140637102248 3 0.11548681242502234 4 0.12603707826598917 5 0.1286103138369567 6 0.07230791954418735 7 0.08208621471386393 8 0.07956444385431576 9 0.0791012614515416 10-11 0.07750585539754173 12-13 0.08113411755260594 14-15 0.07467529626947747 16-17 0.07627070232347734 18-19 0.0856372798017991 20-21 0.08350149427789606 22-23 0.09428335132024997 24-25 0.10781857042353914 26-27 0.10933677941040998 28-29 0.10218318452312027 30-31 0.09279087468908881 32-33 0.09057789209805674 34-35 0.33742838042097106 36-37 0.5265869272427935 38-39 0.508008166420408 40-41 0.480989192925249 42-43 0.5076736457961822 44-45 0.5173747438987298 46-47 0.5142611288578591 48-49 0.5205398236510198 50-51 0.5211831325437617 52-53 0.5188414881741813 54-55 0.5130517081395044 56-57 0.4851578345502165 58-59 0.4760485806289914 60-61 0.4257160928608667 62-63 0.5001855302846667 64-65 0.5258406889272129 66-67 0.5359277723654056 68-69 0.5509040033884366 70-71 0.547224276521953 72-73 0.5326597631902769 74-75 0.5375746431308248 76-77 0.5118422874211497 78-79 0.4868304376713453 80-81 0.49298047068595774 82-83 0.5078280399304403 84-85 0.5013434862916022 86-87 0.502630104077086 88-89 0.5112761755955368 90-91 0.5312187512705351 92-93 0.5377033049093732 94-95 0.5311672865591157 96-97 0.5328656220359542 98-99 0.5338949162643413 100-101 0.5092690518501821 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1943079.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.314569842243046 #Duplication Level Percentage of deduplicated Percentage of total 1 73.07358275944628 36.03592300613641 2 14.498277567028083 14.29952643342864 3 5.2317044567540085 7.739977644797093 4 2.4502515433249044 4.833324034574392 5 1.2970847458752375 3.198258814588624 6 0.7943256928227843 2.3503097913718376 7 0.5174142053668885 1.7861241277553879 8 0.34871926381614893 1.3757552392637644 9 0.265996825360636 1.180576711985581 >10 1.3632833635274446 12.250037042248938 >50 0.09744555958153242 3.307944653705093 >100 0.051143268271679955 4.679421301301537 >500 0.005646800354581058 1.933764615396142 >1k 0.004810237339087567 4.084941738286087 >5k 3.1371113081005874E-4 0.9441148451604727 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6493 0.3341603712458423 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6362 0.3274184940499074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5453 0.2806370713697178 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4021 0.20693960461720803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3389 0.1744139070001786 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3130 0.16108454674256684 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2738 0.14091037986618146 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2525 0.12994839633385982 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2443 0.12572828999747307 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2408 0.12392702509779582 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2351 0.12099353654689285 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2340 0.12042742472127999 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2193 0.11286211214263549 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2062 0.10612023494670057 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2029 0.10442189946986201 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 1991 0.1024662404359267 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1957 0.10071644024766876 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5439413425805126E-4 2 0.0 0.0 0.0 5.1464711419350426E-5 1.5439413425805126E-4 3 0.0 0.0 0.0 5.1464711419350426E-5 1.5439413425805126E-4 4 0.0 0.0 0.0 5.1464711419350426E-5 1.5439413425805126E-4 5 0.0 0.0 0.0 5.1464711419350426E-5 1.5439413425805126E-4 6 0.0 0.0 0.0 5.1464711419350426E-5 1.5439413425805126E-4 7 0.0 0.0 0.0 5.1464711419350426E-5 1.5439413425805126E-4 8 0.0 0.0 0.0 5.1464711419350426E-5 1.5439413425805126E-4 9 0.0 0.0 0.0 5.1464711419350426E-5 1.5439413425805126E-4 10-11 0.0 0.0 0.0 5.1464711419350426E-5 1.5439413425805126E-4 12-13 0.0 0.0 0.0 5.1464711419350426E-5 4.117176913548034E-4 14-15 0.0 0.0 0.0 5.1464711419350426E-5 4.374500470644786E-4 16-17 0.0 0.0 0.0 1.2866177854837607E-4 4.631824027741538E-4 18-19 0.0 0.0 0.0 1.5439413425805126E-4 4.631824027741538E-4 20-21 0.0 0.0 0.0 2.0585884567740168E-4 6.17576537032205E-4 22-23 0.0 0.0 0.0 5.146471141935043E-4 6.690412484515555E-4 24-25 0.0 0.0 0.0 8.234353827096068E-4 7.205059598709059E-4 26-27 0.0 0.0 0.0 0.0016726031211288888 7.719706712902563E-4 28-29 0.0 0.0 0.0 0.0037054592221932303 7.719706712902563E-4 30-31 0.0 0.0 0.0 0.00918645098835405 7.719706712902563E-4 32-33 0.0 0.0 0.0 0.019556590339353162 8.234353827096068E-4 34-35 0.0 0.0 0.0 0.03461001842951316 8.749000941289572E-4 36-37 0.0 0.0 0.0 0.058206588615285326 8.749000941289572E-4 38-39 0.0 0.0 0.0 0.09569863088428211 9.520971612579828E-4 40-41 0.0 0.0 0.0 0.13663880881837537 9.77829516967658E-4 42-43 0.0 0.0 0.0 0.18048674294766193 9.77829516967658E-4 44-45 0.0 0.0 0.0 0.23882199334149562 0.0010035618726773332 46-47 0.0 0.0 0.0 0.30845374789187674 0.0010550265840966835 48-49 0.0 0.0 0.0 0.3839010148326445 0.0011064912955160342 50-51 0.0 0.0 0.0 0.4710050389098951 0.0011836883626450598 52-53 0.0 0.0 0.0 0.5588553013027262 0.0011836883626450598 54-55 0.0 0.0 0.0 0.6530614555558472 0.00123515307406441 56-57 0.0 0.0 0.0 0.7745439068612239 0.0012608854297740855 58-59 0.0 0.0 0.0 0.9060105121819546 0.0012866177854837606 60-61 0.0 0.0 0.0 1.057291031399135 0.0012866177854837606 62-63 0.0 0.0 0.0 1.209883900757509 0.0013895472083224614 64-65 0.0 0.0 0.0 1.3677004383249471 0.0013895472083224614 66-67 0.0 0.0 0.0 1.558016941153705 0.0013895472083224614 68-69 0.0 0.0 0.0 1.7774367382901057 0.0013895472083224614 70-71 0.0 0.0 0.0 2.021739723397762 0.0014410119197418119 72-73 0.0 0.0 0.0 2.2739940064197084 0.0015439413425805126 74-75 0.0 0.0 0.0 2.5409929292632984 0.0016726031211288888 76-77 0.0 0.0 0.0 2.8315626899369506 0.001698335476838564 78-79 0.0 0.0 0.0 3.183941569025243 0.001698335476838564 80-81 0.0 0.0 0.0 3.5655524041997264 0.001698335476838564 82-83 0.0 0.0 0.0 3.957198858100983 0.0017498001882579144 84-85 0.0 0.0 0.0 4.365391216723561 0.0017498001882579144 86-87 0.0 0.0 0.0 4.795430345343653 0.0018527296110966152 88-89 0.0 0.0 0.0 5.256142441969678 0.0019813913896449915 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1395 0.0 25.464819 5 AGGTAAG 1180 0.0 24.484442 2 GTGTAAG 875 0.0 23.87729 1 GTAAGGT 1380 0.0 23.682281 4 TACATGG 8295 0.0 22.782368 2 GTACATG 8600 0.0 22.526936 1 GAGTACT 7445 0.0 22.097172 12-13 ACATGGG 8435 0.0 21.394072 3 GGTAAGG 1495 0.0 21.226929 3 GTACTTT 7845 0.0 20.910404 14-15 AAGGTAA 1325 0.0 20.785118 1 AGTACTT 7830 0.0 20.164183 12-13 GTATCAA 18385 0.0 19.473682 1 TATAGGG 570 0.0 19.111525 2 CATGGGG 3835 0.0 18.772917 4 TAAGGTA 755 0.0 18.19301 4 ACTTTTT 9185 0.0 18.117737 16-17 CATGGGA 4465 0.0 17.82138 4 GTATAGG 560 0.0 17.80622 1 AGAGTAC 14345 0.0 17.70535 10-11 >>END_MODULE