##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939110_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943079 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52342956719722 34.0 31.0 34.0 31.0 34.0 2 32.74006512344583 34.0 31.0 34.0 31.0 34.0 3 32.83770294465639 34.0 31.0 34.0 31.0 34.0 4 36.28087998480762 37.0 37.0 37.0 35.0 37.0 5 36.24958635238197 37.0 37.0 37.0 35.0 37.0 6 36.291495096184974 37.0 37.0 37.0 35.0 37.0 7 36.254636069866436 37.0 37.0 37.0 35.0 37.0 8 36.22476234882885 37.0 37.0 37.0 35.0 37.0 9 37.973662933931145 39.0 38.0 39.0 35.0 39.0 10-11 37.896996467976855 39.0 38.0 39.0 35.0 39.0 12-13 37.820960187413895 39.0 38.0 39.0 35.0 39.0 14-15 39.224637289580095 40.5 39.0 41.0 36.0 41.0 16-17 39.32294646795113 41.0 39.0 41.0 36.0 41.0 18-19 39.374332438362 41.0 39.0 41.0 36.0 41.0 20-21 39.34329329893432 41.0 39.0 41.0 36.0 41.0 22-23 39.275149903838184 41.0 39.0 41.0 36.0 41.0 24-25 39.196242664348695 41.0 39.0 41.0 36.0 41.0 26-27 39.03827816573593 40.5 39.0 41.0 35.0 41.0 28-29 38.88932616738691 40.0 38.0 41.0 35.0 41.0 30-31 38.68023070600835 40.0 38.0 41.0 35.0 41.0 32-33 38.54642528687717 40.0 38.0 41.0 35.0 41.0 34-35 38.42436694545101 40.0 38.0 41.0 34.0 41.0 36-37 38.24669892474778 40.0 38.0 41.0 34.0 41.0 38-39 38.09222064568657 40.0 38.0 41.0 33.5 41.0 40-41 37.89670620700445 40.0 38.0 41.0 33.0 41.0 42-43 37.689258131038414 40.0 37.0 41.0 33.0 41.0 44-45 37.4102146644578 40.0 37.0 41.0 32.5 41.0 46-47 37.07286399575107 40.0 37.0 41.0 31.5 41.0 48-49 36.67368696795138 40.0 36.0 41.0 31.0 41.0 50-51 36.38843402661446 39.5 36.0 41.0 30.0 41.0 52-53 36.094774839314304 39.0 35.0 41.0 30.0 41.0 54-55 35.82266264006765 39.0 35.0 40.0 29.0 41.0 56-57 35.55632632538358 39.0 35.0 40.0 28.0 41.0 58-59 35.200335652847876 38.0 35.0 40.0 27.5 41.0 60-61 34.79382953549495 38.0 34.0 40.0 26.5 41.0 62-63 34.390739131038934 37.0 34.0 40.0 26.0 41.0 64-65 33.92644972232215 36.5 34.0 39.5 25.5 41.0 66-67 33.48994662594779 36.0 33.0 39.0 24.5 40.5 68-69 32.984111814290614 35.5 33.0 39.0 23.0 40.0 70-71 32.45291133299264 35.0 32.0 38.0 22.0 40.0 72-73 31.875314127732324 35.0 31.5 37.0 20.0 39.5 74-75 31.344693396408484 35.0 31.0 37.0 18.0 39.0 76-77 29.368084365072136 32.5 28.5 35.0 13.0 37.0 78-79 30.339442451902368 34.0 30.0 35.0 13.0 37.0 80-81 30.360544012878528 34.0 30.5 35.0 11.5 37.0 82-83 29.978084781936296 34.0 30.0 35.0 7.0 37.0 84-85 29.50377750981818 34.0 29.5 35.0 2.0 36.0 86-87 28.822236512257092 34.0 29.0 35.0 2.0 36.0 88-89 28.11996115443582 33.0 28.0 35.0 2.0 35.0 90-91 27.5519958272412 33.0 26.5 35.0 2.0 35.0 92-93 27.117017887589746 33.0 26.0 35.0 2.0 35.0 94-95 26.581344350898753 32.5 25.0 34.0 2.0 35.0 96-97 25.76930325529739 32.0 23.5 34.0 2.0 35.0 98-99 24.538403482308233 32.0 10.5 34.0 2.0 35.0 100-101 18.625635653516916 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16252598021432618 1101 2 0.12092709276964086 1101 3 0.18066136168891234 1101 4 0.12797764278591472 1101 5 0.14097688887788706 1101 6 0.11763579210429498 1101 7 0.11689794922664731 1101 8 0.1791450339095917 1101 9 0.26142555961586567 1101 10-11 0.18301138208144607 1101 12-13 0.07986194586786155 1101 14-15 0.2593368884321876 1101 16-17 0.1751145348991301 1101 18-19 0.18552705195048702 1101 20-21 0.24302862087338895 1101 22-23 0.19415626529135466 1101 24-25 0.2773074142423866 1101 26-27 0.16111350372950284 1101 28-29 0.2510297084057669 1101 30-31 0.2099125939218922 1101 32-33 0.22200379678935178 1101 34-35 0.11887668704778065 1101 36-37 0.2785883984524631 1101 38-39 0.33116455392291755 1101 40-41 0.4015609667961897 1101 42-43 0.4591115831999062 1101 44-45 0.3965120252577208 1101 46-47 0.30104795046361943 1101 48-49 0.3771312966759055 1101 50-51 0.3561656332821457 1101 52-53 0.31174049689653316 1101 54-55 0.3089526032064285 1101 56-57 0.20661030073572562 1101 58-59 0.32676053851572107 1101 60-61 0.462181183580725 1101 62-63 0.469489817989178 1101 64-65 0.3251988489445097 1101 66-67 0.3324984945616549 1101 68-69 0.4163532705625812 1101 70-71 0.39133134860928465 1101 72-73 0.4484651100862642 1101 74-75 0.22463206274028025 1101 76-77 0.47140248350093117 1101 78-79 0.6594540167759284 1101 80-81 0.5726666099052764 1101 82-83 0.1651848994559657 1101 84-85 0.13979186553698497 1101 86-87 0.4068880856289958 1101 88-89 0.5444331151421977 1101 90-91 0.6125087360421375 1101 92-93 0.5041464755919378 1101 94-95 0.27740652866937765 1101 96-97 0.5173934671094713 1101 98-99 0.33261325376901496 1101 100-101 -0.5647755211962462 1102 1 -0.01292235479096604 1102 2 0.0712487932467667 1102 3 0.07038303508466726 1102 4 0.051845503168344464 1102 5 0.10820806623319612 1102 6 0.07453236893167059 1102 7 0.142004272726723 1102 8 0.14630013590880964 1102 9 0.15088042326989637 1102 10-11 0.12913365010256683 1102 12-13 0.18259134036626534 1102 14-15 0.13807022283324244 1102 16-17 0.08480999323676741 1102 18-19 0.08905949645188116 1102 20-21 0.14879819043975573 1102 22-23 0.1208349098832997 1102 24-25 0.18768827877875083 1102 26-27 0.0949718055528308 1102 28-29 0.23190494627775848 1102 30-31 0.18010634220200217 1102 32-33 0.1502203283646253 1102 34-35 0.16888130980864702 1102 36-37 0.22879143593394957 1102 38-39 0.2672758146772054 1102 40-41 0.3643319079029439 1102 42-43 0.36100331158831267 1102 44-45 0.3060638868107688 1102 46-47 0.2362568628553845 1102 48-49 0.2432175581217848 1102 50-51 0.06612797416089933 1102 52-53 0.31046541568881736 1102 54-55 0.2250054497351286 1102 56-57 0.485053383993467 1102 58-59 0.23569087532935384 1102 60-61 0.12397763213558477 1102 62-63 0.26673316012153236 1102 64-65 0.3916415725732918 1102 66-67 0.42023849593028473 1102 68-69 0.40312180055374114 1102 70-71 0.20404255782160874 1102 72-73 0.26824568546347294 1102 74-75 -0.029401751117521968 1102 76-77 0.11188873242532082 1102 78-79 0.19392834698519934 1102 80-81 0.36187290006323636 1102 82-83 0.13612465942183505 1102 84-85 0.0740789557310535 1102 86-87 0.27677229092908107 1102 88-89 0.14582827893352857 1102 90-91 0.2983817608128554 1102 92-93 0.17809477929349882 1102 94-95 0.2700798166826637 1102 96-97 0.29553825489198715 1102 98-99 0.37633292372573024 1102 100-101 -0.24314110950990653 1103 1 0.07444762420580275 1103 2 0.10414167334229063 1103 3 0.14666627368236362 1103 4 0.1411831347831125 1103 5 0.16103454236033343 1103 6 0.1774778173996765 1103 7 0.14789137908851302 1103 8 0.14472153404488353 1103 9 0.16688009245614666 1103 10-11 0.18800350762866458 1103 12-13 0.22633370537444364 1103 14-15 0.20322950448496613 1103 16-17 0.24560632396092075 1103 18-19 0.2996674184073953 1103 20-21 0.3091091553648795 1103 22-23 0.30851898964387203 1103 24-25 0.18400676971668872 1103 26-27 0.3306936601085013 1103 28-29 0.32624113919730746 1103 30-31 0.259987869527734 1103 32-33 0.2933265660990969 1103 34-35 0.32109939877341986 1103 36-37 0.3339648805493596 1103 38-39 0.45932456835363666 1103 40-41 0.5648426016297137 1103 42-43 0.648675158132086 1103 44-45 0.49387495080772226 1103 46-47 0.5021146439566095 1103 48-49 0.27906071105250163 1103 50-51 0.12741156597118675 1103 52-53 0.26716103430191396 1103 54-55 0.3228269033514195 1103 56-57 0.33574438844394905 1103 58-59 0.28147567064696233 1103 60-61 0.5492755798842381 1103 62-63 0.490633764373527 1103 64-65 0.46924496479262245 1103 66-67 0.2986501419526846 1103 68-69 0.08494582486854796 1103 70-71 0.06942996312186978 1103 72-73 0.10459660304087848 1103 74-75 0.21126318452025217 1103 76-77 0.218417996519797 1103 78-79 0.1564983218468008 1103 80-81 0.2068086067554944 1103 82-83 0.2533366231632712 1103 84-85 0.07716655073231493 1103 86-87 0.23376598651495684 1103 88-89 0.39495015795563404 1103 90-91 0.46009592739481064 1103 92-93 0.3681564105843407 1103 94-95 0.22920950084168368 1103 96-97 -0.20429995650833987 1103 98-99 -0.14849430362429672 1103 100-101 -0.5799536156480549 1104 1 0.09904866572512105 1104 2 0.11365483050117575 1104 3 0.12715143877600354 1104 4 0.06155987133399776 1104 5 0.07336861806950878 1104 6 0.1128717097409222 1104 7 0.12277747319369325 1104 8 0.09429502431888181 1104 9 0.15568030562877055 1104 10-11 0.1629257884441131 1104 12-13 0.18828008982863054 1104 14-15 0.10470888299466452 1104 16-17 0.12549128311928115 1104 18-19 0.24748305698769002 1104 20-21 0.22416480065616895 1104 22-23 0.20164466608863307 1104 24-25 0.202508541220638 1104 26-27 0.24727737148329965 1104 28-29 0.1027725772242789 1104 30-31 0.15803378717335903 1104 32-33 0.14816851241776163 1104 34-35 0.15797447895988626 1104 36-37 0.20818283639498958 1104 38-39 0.13258258946693502 1104 40-41 0.17193601926711466 1104 42-43 0.2196603248705813 1104 44-45 0.17289899523184715 1104 46-47 0.1538930562811558 1104 48-49 0.2520989801434581 1104 50-51 0.1924073387054861 1104 52-53 0.19153689579235333 1104 54-55 0.11795633663464145 1104 56-57 0.01417612221502651 1104 58-59 0.024944627384101636 1104 60-61 0.05771267586784745 1104 62-63 0.17510045450672607 1104 64-65 0.19363546545220345 1104 66-67 0.2768438658612453 1104 68-69 0.22895873365501274 1104 70-71 0.14075660081724095 1104 72-73 -1.0191231708844839E-4 1104 74-75 -0.0876130536409363 1104 76-77 -0.051712176946132615 1104 78-79 0.12301591023284075 1104 80-81 0.20704743904325085 1104 82-83 0.1467919276415408 1104 84-85 0.03637096945860918 1104 86-87 0.0724015501558668 1104 88-89 0.22177964288715657 1104 90-91 0.46631395561386313 1104 92-93 0.2416917534863181 1104 94-95 0.22383232018117738 1104 96-97 -0.2476920987286917 1104 98-99 -0.21430131660874352 1104 100-101 -0.7525939954111287 1105 1 -0.07366927083666752 1105 2 0.022785168166656433 1105 3 0.029802436156138867 1105 4 0.01595474542985187 1105 5 0.019392024315912693 1105 6 0.07949882548208365 1105 7 0.1095990049606641 1105 8 0.06534426782602765 1105 9 0.08580248152011194 1105 10-11 0.06157827842668695 1105 12-13 0.10366574716957189 1105 14-15 0.12877546921362182 1105 16-17 0.18516526149738155 1105 18-19 0.23156245275721687 1105 20-21 0.17827443920803177 1105 22-23 0.24870641437412644 1105 24-25 0.19116473014815938 1105 26-27 0.23929663725574812 1105 28-29 0.11477965861995187 1105 30-31 0.13839063116579808 1105 32-33 0.22395162399891433 1105 34-35 0.2595461723928949 1105 36-37 0.20473851951783928 1105 38-39 0.25405353223553107 1105 40-41 0.28869943592005143 1105 42-43 0.2777523204969654 1105 44-45 0.24109031534491976 1105 46-47 0.2863528937489903 1105 48-49 0.04157007660061396 1105 50-51 0.05830446635750519 1105 52-53 0.16076483220435733 1105 54-55 0.07484457965532698 1105 56-57 0.07569420048288578 1105 58-59 -0.11485872289620858 1105 60-61 -0.2633645376543967 1105 62-63 -0.2745652403185943 1105 64-65 -0.007118910927161437 1105 66-67 -0.18608088600662853 1105 68-69 -0.04478924185640665 1105 70-71 0.005331257964762415 1105 72-73 -0.3623297256480065 1105 74-75 -0.21763389927966514 1105 76-77 -0.38543874025507563 1105 78-79 -0.23972506658818915 1105 80-81 -0.08615581472585987 1105 82-83 -0.11784593066933269 1105 84-85 -0.13843446147824423 1105 86-87 -0.0858136568059642 1105 88-89 0.1484828995867744 1105 90-91 0.027599710182350634 1105 92-93 -0.10132254801546736 1105 94-95 0.05717449928956242 1105 96-97 -0.1268554468729981 1105 98-99 -0.421146090513993 1105 100-101 -0.9274083806601503 1106 1 0.13363574392626276 1106 2 0.10216363084808222 1106 3 0.1300381858586661 1106 4 0.03598266455896493 1106 5 0.0962686022838497 1106 6 0.06150016412195214 1106 7 0.029981566561119166 1106 8 0.08152195300637288 1106 9 0.15374957361158437 1106 10-11 0.15574426413261477 1106 12-13 0.18125737305950906 1106 14-15 0.09815181149450325 1106 16-17 0.14195575809394256 1106 18-19 0.07184492644091733 1106 20-21 0.11165330216594782 1106 22-23 0.13118689463075128 1106 24-25 0.16464625557119916 1106 26-27 0.13237551834826178 1106 28-29 0.18223736727817652 1106 30-31 0.21100291764977186 1106 32-33 0.2748490670840056 1106 34-35 0.12043646499876814 1106 36-37 0.20004498003688553 1106 38-39 0.2357241259758709 1106 40-41 0.3501832214332339 1106 42-43 0.3024931978416632 1106 44-45 0.514903400001522 1106 46-47 0.4671859449364604 1106 48-49 0.4846274561426043 1106 50-51 0.4474568479870058 1106 52-53 0.5315008930764549 1106 54-55 0.5146875322050235 1106 56-57 0.37455291133573354 1106 58-59 0.4287952189659734 1106 60-61 0.3853643339010091 1106 62-63 0.4655087325321361 1106 64-65 0.5404533870577026 1106 66-67 0.40257268470715957 1106 68-69 0.45841312672808954 1106 70-71 0.2245806737583962 1106 72-73 0.20949218701591477 1106 74-75 0.004820860549738626 1106 76-77 0.12370168945646398 1106 78-79 0.3650460538696656 1106 80-81 0.5151172712524641 1106 82-83 0.3086756133205242 1106 84-85 0.22567558261167875 1106 86-87 0.22362278332654384 1106 88-89 -0.029353089154120937 1106 90-91 0.24071452846214925 1106 92-93 0.26331462075808076 1106 94-95 0.13538016436086409 1106 96-97 -0.09968346923461269 1106 98-99 -0.09770028616651416 1106 100-101 -0.7167454908329702 1107 1 0.01727243358988062 1107 2 0.008217419891870748 1107 3 -0.036073220240403714 1107 4 -0.01828101873442023 1107 5 -0.046554572845401765 1107 6 0.05303449764116408 1107 7 0.011006587968360293 1107 8 0.01851060618422906 1107 9 0.034274905942531575 1107 10-11 0.010450055794649415 1107 12-13 0.08893620416810677 1107 14-15 0.0022947752138122723 1107 16-17 -0.014815820965338844 1107 18-19 0.05476017618096307 1107 20-21 0.086563743114219 1107 22-23 0.09900273208171484 1107 24-25 0.07979270775452818 1107 26-27 0.10748496258387519 1107 28-29 0.11331634977466365 1107 30-31 0.08264519152130134 1107 32-33 -0.00892325982781017 1107 34-35 -0.0901711068794313 1107 36-37 0.023718223302807928 1107 38-39 0.032993086036796626 1107 40-41 0.0300235826317774 1107 42-43 0.009037104418553099 1107 44-45 0.14425508242741358 1107 46-47 0.16374500544709036 1107 48-49 0.0764555088212262 1107 50-51 0.08336958095640767 1107 52-53 0.1004953630870844 1107 54-55 -0.022758555926472468 1107 56-57 -0.061145400892250734 1107 58-59 -0.06311894731172885 1107 60-61 -0.013331634207581544 1107 62-63 -0.16178824852438112 1107 64-65 -0.26284201045363886 1107 66-67 -0.2281464259389665 1107 68-69 -0.0028943642463374886 1107 70-71 -0.011220653717714413 1107 72-73 -0.21630018379778093 1107 74-75 -0.3599367174015491 1107 76-77 -0.252379690469283 1107 78-79 -0.19540217907504243 1107 80-81 0.02150021839725369 1107 82-83 -0.02608062885526863 1107 84-85 -0.03626544759532635 1107 86-87 -0.0959004690111378 1107 88-89 -0.3155605559876591 1107 90-91 -0.09866816152313973 1107 92-93 -0.028257605167897992 1107 94-95 -0.03626319993964344 1107 96-97 -0.2345704417282377 1107 98-99 -0.10185073144015533 1107 100-101 -0.6804867586929078 1108 1 -0.1255658773468724 1108 2 -0.04792931482134577 1108 3 -0.02782779313532302 1108 4 -0.05111797984506694 1108 5 -0.023639695176981945 1108 6 0.03397581466936117 1108 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-0.8367682525589402 2316 50-51 -0.7205158834325331 2316 52-53 -0.8258902584939847 2316 54-55 -0.8807496621038027 2316 56-57 -0.7136683888871076 2316 58-59 -0.700802889213513 2316 60-61 -0.6196276671116934 2316 62-63 -0.6022074420094796 2316 64-65 -0.5148496250575434 2316 66-67 -0.6134805080127776 2316 68-69 -0.44625738701859774 2316 70-71 -0.4172020855321108 2316 72-73 -0.18794901006035758 2316 74-75 -0.451610412544202 2316 76-77 -0.3732278125110895 2316 78-79 -0.5090888441950803 2316 80-81 -0.45143855905987706 2316 82-83 -0.5635705197747676 2316 84-85 -0.6706946450048008 2316 86-87 -0.5938986000508386 2316 88-89 -0.8642087036138442 2316 90-91 -1.0688852384432153 2316 92-93 -1.023329143851253 2316 94-95 -1.0191919171000343 2316 96-97 -0.861420809991472 2316 98-99 -0.655791267281046 2316 100-101 0.3089185997160051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 6.0 5 26.0 6 65.0 7 165.0 8 452.0 9 955.0 10 1657.0 11 2668.0 12 4019.0 13 5553.0 14 7049.0 15 10095.0 16 13410.0 17 19154.0 18 19064.0 19 13369.0 20 12304.0 21 12124.0 22 12835.0 23 13722.0 24 15593.0 25 17988.0 26 20853.0 27 24336.0 28 29527.0 29 36213.0 30 43481.0 31 54186.0 32 68453.0 33 89480.0 34 125879.0 35 192210.0 36 314210.0 37 462888.0 38 285941.0 39 13145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.029908697155385 19.51788873218401 15.491250854656021 21.960951716004587 2 15.598130595822404 21.2439638326594 39.0625908673811 24.095314704137095 3 19.427980025516202 24.763326658360263 30.586249967191247 25.222443348932288 4 13.006625052301013 17.65913789403313 37.490704186499876 31.843532867165976 5 13.414431425587948 37.920280132717195 34.1489460799072 14.516342361787657 6 30.670137446804784 37.234512853054355 17.493524452685662 14.6018252474552 7 28.107040423986877 33.56806388211699 20.502100017549466 17.822795676346665 8 26.73941718272906 33.77438591019717 19.793173617747915 19.693023289325858 9 25.994311090799705 17.535828445472365 20.642650144435713 35.82721031929222 10-11 24.5932100547636 26.829300301222958 27.284917391418468 21.29257225259498 12-13 26.574189726717236 24.114794097409316 27.480380365389156 21.83063581048429 14-15 22.988274449973225 25.70874512915037 26.490396999173214 24.81258342170319 16-17 21.632683310563966 28.901416830220978 27.34797040402525 22.11792945518981 18-19 21.62850815638479 27.351049545592332 29.73672197579203 21.283720322230852 20-21 22.543833781333646 25.939037990735326 29.875702428979984 21.641425798951047 22-23 22.078747184236978 25.910449343541874 30.065916002385904 21.944887469835244 24-25 22.34220654034475 26.23476772419841 29.896200770118337 21.5268249653385 26-27 21.76692243022472 26.242540229556738 30.111571995035526 21.878965345183016 28-29 21.646109087690206 27.192795557977828 29.772103964892832 21.388991389439134 30-31 21.814836144078548 26.360173724279868 29.537347683753467 22.28764244788812 32-33 21.546319012248087 26.49223217378192 29.54246842253969 22.418980391430303 34-35 22.69110872229944 26.44777385428186 29.166403951713292 21.694713471705406 36-37 22.705868332706586 25.859833658546755 29.50600763267022 21.92829037607644 38-39 22.01130388455213 26.437963756534096 29.506381598758423 22.04435076015535 40-41 22.40743933728444 26.440786138353968 29.45746468579428 21.694309838567307 42-43 21.923097906647342 26.62367106693458 28.83918571710916 22.614045309308914 44-45 22.23031391002306 26.5232945395518 29.0650995440577 22.181292006367443 46-47 22.976591339837952 27.029005739901773 28.11993448199992 21.87446843826035 48-49 22.452413651837436 27.109647067542518 28.032694456903567 22.405244823716476 50-51 22.344471838171096 27.275497030592653 28.432561039533173 21.94747009170308 52-53 22.881493752956004 27.107595728223092 27.96896060324876 22.04194991557214 54-55 22.158105769245616 27.058343999394772 28.233257628742837 22.550292602616775 56-57 22.51116850580433 27.349606332847078 27.75936910325924 22.379856058089352 58-59 22.6530522967816 26.790012583342126 27.850178457009488 22.706756662866788 60-61 22.001162098900895 27.1313809934556 28.565965839027175 22.30149106861633 62-63 22.130147762310138 27.098411623357677 28.622482248296883 22.148958366035306 64-65 21.742319106936396 27.478365502765158 28.22496120085259 22.55435418944586 66-67 21.760913909748993 27.265982908710157 27.990699093918202 22.982404087622648 68-69 22.266523862926938 26.94178517369852 28.22246400270099 22.569226960673557 70-71 22.351221646102186 27.02106197650631 27.773734930458165 22.853981446933343 72-73 22.103373048651136 26.868670805458763 28.118079604586327 22.909876541303774 74-75 21.96192228931505 27.954190231071408 27.39446002967455 22.68942744993899 76-77 22.13320235341449 27.093062279766617 27.814499260662316 22.95923610615658 78-79 22.126203010373008 27.34904704978555 27.91774169264959 22.60700824719185 80-81 22.542107924434113 27.35111530835828 27.487677376937814 22.619099390269792 82-83 22.367773003568047 27.273569422550498 27.80262665594142 22.55603091794003 84-85 22.090841391420525 27.57687669930044 27.660686981846855 22.671594927432185 86-87 22.446142277793506 27.481123489512076 27.358118013643175 22.714616219051244 88-89 22.17777509027678 27.51068152441259 27.01904973065555 23.29249365465509 90-91 22.026731347144434 27.251983298916272 27.406595207005008 23.31469014693429 92-93 22.26070067146009 27.113179649412096 27.6666568883715 22.959462790756323 94-95 21.86053680781893 26.94934174060859 27.788113607321165 23.402007844251315 96-97 21.809721017447316 26.560301089225163 28.036952701602125 23.5930251917254 98-99 21.74176073582375 27.086461966965746 27.237228227025835 23.934549070184666 100-101 22.04956893002421 25.691636748406648 28.37491353897334 23.883880782595806 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 413.0 1 278.5 2 188.0 3 238.5 4 211.0 5 199.0 6 354.5 7 816.0 8 1372.0 9 1672.0 10 1555.0 11 1348.5 12 1427.0 13 1574.0 14 1705.5 15 1915.0 16 2090.0 17 2291.0 18 2626.0 19 2985.5 20 3399.0 21 4090.0 22 4977.0 23 6065.0 24 7469.0 25 9314.5 26 12291.0 27 15956.0 28 18616.0 29 21591.5 30 25249.0 31 30011.5 32 37350.0 33 40360.0 34 40180.0 35 42347.5 36 45964.5 37 49949.5 38 52672.5 39 54140.0 40 55625.0 41 58500.5 42 59178.5 43 59234.5 44 60978.5 45 64744.0 46 67735.0 47 73595.0 48 88012.5 49 85944.5 50 71224.0 51 64748.5 52 62297.0 53 63333.0 54 64504.0 55 60781.5 56 55220.5 57 49498.0 58 45666.5 59 41115.5 60 32548.5 61 27233.5 62 23514.5 63 16774.0 64 10652.5 65 7843.0 66 6148.5 67 5063.5 68 3993.5 69 2940.5 70 2176.5 71 1440.0 72 1022.5 73 848.0 74 620.5 75 456.5 76 345.0 77 197.0 78 114.0 79 70.0 80 39.0 81 22.0 82 12.5 83 8.0 84 2.5 85 3.0 86 3.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41634951538254494 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.462383155805811E-4 16-17 0.002290179658161094 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.146471141935042E-5 26-27 0.003782656289322256 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01160529242506352 36-37 0.02616980575673969 38-39 0.01976244918503056 40-41 0.00844021267277347 42-43 0.008594606807031521 44-45 0.0036025297993545293 46-47 0.005223668209064068 48-49 0.0030106856180319996 50-51 7.462383155805811E-4 52-53 0.0 54-55 0.0 56-57 1.8012648996772648E-4 58-59 0.0017498001882579142 60-61 0.0022129825910320684 62-63 0.0013380824969031109 64-65 0.0030621503294513503 66-67 0.002187250235322393 68-69 0.004425965182064137 70-71 2.0585884567740168E-4 72-73 0.0 74-75 0.0 76-77 0.09338271887041134 78-79 0.015259286935837401 80-81 3.345206242257777E-4 82-83 0.0 84-85 0.0 86-87 0.10851334402770037 88-89 0.4218047747929961 90-91 0.42378616618264103 92-93 0.0 94-95 0.0 96-97 2.573235570967521E-5 98-99 2.573235570967521E-4 100-101 3.6025297993545297E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1943079.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.10449851562359 #Duplication Level Percentage of deduplicated Percentage of total 1 72.10004034310535 34.683362836613114 2 14.785355083003592 14.22484183286628 3 5.4768113658233215 7.903777926527949 4 2.629160548684778 5.05897798846172 5 1.389265604638206 3.3414962608062746 6 0.8200810998964055 2.366975403159456 7 0.5576957021021158 1.877937045175862 8 0.39012201187623907 1.50132989929701 9 0.269125719729382 1.1651542006714366 >10 1.4124037239240077 12.47184344905846 >50 0.1016460234178836 3.347748001068375 >100 0.05739323198219039 5.075325796311967 >500 0.0057703456675964535 1.9585809874976747 >1k 0.004808621389663711 4.027988194328849 >5k 3.2057475931091407E-4 0.9946601781554775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6815 0.3507320083228731 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6653 0.34239472507293833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5882 0.30271543256861916 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3696 0.19021357340591916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3569 0.18367755505566163 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2853 0.14682882167940675 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2844 0.1463656392766326 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2518 0.12958814335392435 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2423 0.12469899576908607 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2395 0.12325798384934426 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2266 0.11661903607624806 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2199 0.11317090041115159 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2170 0.11167842377999042 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2130 0.10961983532321641 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2116 0.1088993293633455 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2091 0.10761271157786173 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2050 0.10550265840966837 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.1464711419350426E-5 2 0.0 0.0 0.0 0.0 5.1464711419350426E-5 3 0.0 0.0 0.0 0.0 5.1464711419350426E-5 4 0.0 0.0 0.0 0.0 5.1464711419350426E-5 5 0.0 0.0 0.0 0.0 5.1464711419350426E-5 6 0.0 0.0 0.0 0.0 1.0292942283870085E-4 7 0.0 0.0 0.0 0.0 1.0292942283870085E-4 8 0.0 0.0 0.0 0.0 1.0292942283870085E-4 9 0.0 0.0 0.0 0.0 1.0292942283870085E-4 10-11 0.0 0.0 0.0 0.0 1.5439413425805126E-4 12-13 0.0 0.0 0.0 0.0 3.345206242257778E-4 14-15 0.0 0.0 0.0 0.0 4.117176913548034E-4 16-17 0.0 0.0 0.0 5.1464711419350426E-5 4.889147584838289E-4 18-19 0.0 0.0 0.0 5.1464711419350426E-5 5.146471141935042E-4 20-21 0.0 0.0 0.0 1.0292942283870085E-4 5.661118256128547E-4 22-23 0.0 0.0 0.0 3.087882685161025E-4 5.661118256128547E-4 24-25 0.0 0.0 0.0 5.661118256128547E-4 5.661118256128547E-4 26-27 0.0 0.0 0.0 0.0015182089868708375 6.947736041612307E-4 28-29 0.0 0.0 0.0 0.0036025297993545298 7.719706712902563E-4 30-31 0.0 0.0 0.0 0.0091349862769347 7.719706712902563E-4 32-33 0.0 0.0 0.0 0.019633787406482188 7.719706712902563E-4 34-35 0.0 0.0 0.0 0.03481587727519056 7.719706712902563E-4 36-37 0.0 0.0 0.0 0.05872123572947883 7.719706712902563E-4 38-39 0.0 0.0 0.0 0.09672792511266912 7.719706712902563E-4 40-41 0.0 0.0 0.0 0.13789969424814946 7.719706712902563E-4 42-43 0.0 0.0 0.0 0.1820821490016618 7.977030269999316E-4 44-45 0.0 0.0 0.0 0.24067472295259223 8.234353827096068E-4 46-47 0.0 0.0 0.0 0.31092405404000556 8.234353827096068E-4 48-49 0.0 0.0 0.0 0.38683450338354747 0.0010292942283870083 50-51 0.0 0.0 0.0 0.474813427554927 0.0010807589398063588 52-53 0.0 0.0 0.0 0.5632040694176614 0.0010807589398063588 54-55 0.0 0.0 0.0 0.6582593914092016 0.001209420718354735 56-57 0.0 0.0 0.0 0.7804880810301589 0.00123515307406441 58-59 0.0 0.0 0.0 0.9128295864450184 0.00123515307406441 60-61 0.0 0.0 0.0 1.0649592734006181 0.00123515307406441 62-63 0.0 0.0 0.0 1.2185042399202501 0.00123515307406441 64-65 0.0 0.0 0.0 1.3776588599845914 0.00123515307406441 66-67 0.0 0.0 0.0 1.5693649100216718 0.00123515307406441 68-69 0.0 0.0 0.0 1.7901999867221043 0.00123515307406441 70-71 0.0 0.0 0.0 2.0369732779778897 0.00123515307406441 72-73 0.0 0.0 0.0 2.2916978671479646 0.0014410119197418119 74-75 0.0 0.0 0.0 2.5602407313341353 0.001698335476838564 76-77 0.0 0.0 0.0 2.852611756907465 0.0017498001882579144 78-79 0.0 0.0 0.0 3.2058398037341767 0.0017498001882579144 80-81 0.0 0.0 0.0 3.5860353593446277 0.00182699725538694 82-83 2.5732355709675213E-5 0.0 0.0 3.9770899690645622 0.0018527296110966152 84-85 5.1464711419350426E-5 0.0 0.0 4.38605429835843 0.0018527296110966152 86-87 5.1464711419350426E-5 0.0 0.0 4.81707125649549 0.0018784619668062905 88-89 5.1464711419350426E-5 0.0 0.0 5.278452394369967 0.001955659033935316 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 245 0.0 26.147427 94-95 TAAGGTG 1280 0.0 25.20862 5 AGGTAAG 1220 0.0 23.336813 2 GTAAGGT 1325 0.0 22.91998 4 GAGTACT 7620 0.0 22.32578 12-13 GGTGTGC 1570 0.0 21.761208 8 ACCGTAT 110 0.0028951776 21.568872 8 TACATGG 8330 0.0 21.532621 2 GTACATG 8815 0.0 21.299074 1 GTACTTT 8125 0.0 20.908945 14-15 ACATGGG 8360 0.0 20.830992 3 GGTAAGG 1440 0.0 20.76004 3 AGTACTT 8070 0.0 20.551622 12-13 TAAGGTA 605 0.0 20.392387 4 AAGGTAA 1320 0.0 19.855259 1 GTGTAAG 870 0.0 19.718327 1 CATGGGA 4405 0.0 19.282228 4 GTATCAA 19535 0.0 18.636602 1 AGAGTAC 15290 0.0 17.814632 10-11 TACTTTT 9195 0.0 17.804913 14-15 >>END_MODULE