##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939109_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69360 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.594535755478663 33.0 31.0 34.0 30.0 34.0 2 31.55222029988466 33.0 31.0 34.0 30.0 34.0 3 31.56875720876586 34.0 31.0 34.0 30.0 34.0 4 34.938148788927336 37.0 35.0 37.0 33.0 37.0 5 34.99365628604383 37.0 35.0 37.0 33.0 37.0 6 35.20653114186851 37.0 35.0 37.0 33.0 37.0 7 35.19988465974625 37.0 35.0 37.0 33.0 37.0 8 35.224322376009226 37.0 35.0 37.0 33.0 37.0 9 36.76114475201845 39.0 37.0 39.0 33.0 39.0 10-11 36.695797289504036 39.0 37.0 39.0 33.0 39.0 12-13 36.66522491349481 39.0 37.0 39.0 33.0 39.0 14-15 37.633153114186854 40.0 38.0 41.0 33.0 41.0 16-17 37.62948385236447 40.0 38.0 41.0 32.5 41.0 18-19 37.674192618223756 40.0 38.0 41.0 33.0 41.0 20-21 37.60226355247981 40.0 38.0 41.0 33.0 41.0 22-23 37.53434256055363 40.0 38.0 41.0 33.0 41.0 24-25 37.43690888119954 40.0 37.5 41.0 32.5 41.0 26-27 37.18306660899654 40.0 36.5 41.0 32.5 41.0 28-29 37.071316320645906 40.0 36.0 41.0 32.5 41.0 30-31 36.88830017301038 40.0 35.5 41.0 33.0 41.0 32-33 36.40733852364475 39.0 35.0 41.0 31.0 41.0 34-35 35.92040801614763 39.0 35.0 41.0 30.0 41.0 36-37 35.523471741637834 38.5 35.0 41.0 30.0 41.0 38-39 35.183311707035756 38.0 35.0 40.5 28.5 41.0 40-41 35.08608708189158 38.0 35.0 41.0 27.5 41.0 42-43 34.70168685121107 38.0 35.0 41.0 24.5 41.0 44-45 34.22280132641292 38.0 35.0 40.5 18.0 41.0 46-47 33.47758794694349 38.0 35.0 40.0 4.5 41.0 48-49 32.944607843137256 38.0 34.0 40.5 2.0 41.0 50-51 31.723140138408304 36.5 32.0 39.0 2.0 40.5 52-53 31.90020905420992 37.5 33.0 39.5 2.0 40.5 54-55 32.240484429065745 38.0 33.0 40.0 2.0 41.0 56-57 31.84354815455594 38.0 33.0 40.5 2.0 41.0 58-59 31.55230680507497 38.0 32.5 40.0 2.0 41.0 60-61 30.86283881199539 37.0 31.0 40.0 2.0 41.0 62-63 30.49506920415225 37.0 31.0 40.0 2.0 41.0 64-65 29.987932525951557 36.0 29.5 39.5 2.0 40.5 66-67 29.28817041522491 36.0 28.5 39.0 2.0 40.0 68-69 28.670530565167244 35.0 27.5 38.0 2.0 39.0 70-71 27.871950692041523 35.0 26.0 37.0 2.0 39.0 72-73 27.232605247981546 34.5 25.0 36.5 2.0 37.5 74-75 26.782626874279124 34.0 22.5 36.0 2.0 37.0 76-77 26.315787197231835 34.0 20.0 35.0 2.0 36.5 78-79 25.802645617070358 34.0 12.5 35.0 2.0 36.0 80-81 25.26882929642445 34.0 2.0 35.0 2.0 36.0 82-83 24.722426470588236 33.5 2.0 35.0 2.0 35.5 84-85 24.271756055363323 33.0 2.0 35.0 2.0 35.0 86-87 24.093822087658594 33.0 2.0 35.0 2.0 35.0 88-89 23.56180795847751 33.0 2.0 35.0 2.0 35.0 90-91 23.06267301038062 32.0 2.0 35.0 2.0 35.0 92-93 22.612882064590544 32.0 2.0 35.0 2.0 35.0 94-95 22.03455161476355 31.0 2.0 35.0 2.0 35.0 96-97 21.416464821222608 31.0 2.0 35.0 2.0 35.0 98-99 20.93657006920415 31.0 2.0 35.0 2.0 35.0 100-101 18.891299019607843 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.103024012300125 1101 2 -0.982397768454291 1101 3 -0.9106973243488632 1101 4 -1.411259998929559 1101 5 -1.3068640091512407 1101 6 -1.282483099020702 1101 7 -1.3710021551975444 1101 8 -1.1754273721447106 1101 9 -1.8257160762809903 1101 10-11 -0.952552622561285 1101 12-13 -1.049485189173815 1101 14-15 -0.7857566530331894 1101 16-17 -1.0398522729008235 1101 18-19 -1.4657125749824402 1101 20-21 -1.0980222417168974 1101 22-23 -1.1862319012505154 1101 24-25 -0.6836729198493572 1101 26-27 -0.7585032057395011 1101 28-29 -0.7600809123032022 1101 30-31 -0.5412942842629462 1101 32-33 -0.6247245812258413 1101 34-35 -0.8343999241493663 1101 36-37 -0.08170142861758478 1101 38-39 -0.37258177361724876 1101 40-41 -0.10198513340483828 1101 42-43 -0.5250116199255146 1101 44-45 0.007727245699086893 1101 46-47 0.4899345312892507 1101 48-49 0.619584255419106 1101 50-51 0.6124107792935654 1101 52-53 0.5256428087481062 1101 54-55 1.198330175112055 1101 56-57 1.5747392939148703 1101 58-59 0.3591875654609886 1101 60-61 0.46751987435214204 1101 62-63 0.9859264497078222 1101 64-65 1.049466234380727 1101 66-67 1.6127956715619725 1101 68-69 0.2871116582544282 1101 70-71 0.6393830113724555 1101 72-73 0.810138507173189 1101 74-75 0.815202955202853 1101 76-77 0.11282688273799124 1101 78-79 -0.2554685511483825 1101 80-81 0.4132527589651538 1101 82-83 -0.002720975604987075 1101 84-85 -0.2596770665290329 1101 86-87 0.035397130254974485 1101 88-89 0.4673199816782194 1101 90-91 0.09012316962515499 1101 92-93 -0.34816309247259625 1101 94-95 0.08076511643589868 1101 96-97 0.775426404630803 1101 98-99 -0.6355205570148179 1101 100-101 -1.266220444813161 1102 1 -1.2434598476512129 1102 2 -1.4155091485995683 1102 3 -1.3144503509832468 1102 4 -1.5008483766535292 1102 5 -1.964854323921216 1102 6 -1.6659576752918852 1102 7 -1.2895856903065024 1102 8 -1.4108995271083913 1102 9 -1.680134962479542 1102 10-11 -1.5445623077913098 1102 12-13 -1.3470033489801025 1102 14-15 -1.282881350369756 1102 16-17 -1.0817711590993753 1102 18-19 -1.1406520422947963 1102 20-21 -1.1541663095135064 1102 22-23 -1.0628965501609287 1102 24-25 -0.8260760675491099 1102 26-27 -1.0526920677249691 1102 28-29 -0.9583254643613159 1102 30-31 -0.8011308460062878 1102 32-33 -0.5254509734776533 1102 34-35 -0.8531650573212843 1102 36-37 -1.0730755206272633 1102 38-39 -0.8477633716802089 1102 40-41 -1.9903023246881304 1102 42-43 -1.4099995133879801 1102 44-45 -1.7505959988529725 1102 46-47 -2.033975880333024 1102 48-49 -2.368309207050629 1102 50-51 -1.227177598430405 1102 52-53 -1.8555617917361502 1102 54-55 -1.8180136505538016 1102 56-57 -1.1717800765451862 1102 58-59 -0.9180521439821092 1102 60-61 -1.8480067600304224 1102 62-63 -1.1446728238999277 1102 64-65 -1.0824950246991776 1102 66-67 -0.9624161928448061 1102 68-69 -0.9946680027625199 1102 70-71 -0.4350721944386855 1102 72-73 -0.07076342987281592 1102 74-75 -0.19009365496663833 1102 76-77 -0.6224939405065619 1102 78-79 -0.33658235260116953 1102 80-81 -0.35445305217286105 1102 82-83 -0.7628602007865872 1102 84-85 -0.4303792457057831 1102 86-87 -0.44538374141572845 1102 88-89 0.06598826254989021 1102 90-91 -0.03790346475741657 1102 92-93 0.2226601520794631 1102 94-95 0.5553413876223416 1102 96-97 0.890438511168334 1102 98-99 1.209969757755161 1102 100-101 0.7858376665669837 1103 1 0.42001746235425585 1103 2 0.6771644435272783 1103 3 1.0144178830244073 1103 4 0.5150072821764269 1103 5 0.9892189401575209 1103 6 0.3909039977534903 1103 7 0.36459692314347336 1103 8 0.48931917163409366 1103 9 0.46748668869596344 1103 10-11 0.5336224926460886 1103 12-13 0.5939479905496867 1103 14-15 0.7277802133262554 1103 16-17 1.1464956533664505 1103 18-19 0.9029510194876025 1103 20-21 0.6257680808637431 1103 22-23 0.6533418314683743 1103 24-25 0.9565920571091695 1103 26-27 0.8267502504817017 1103 28-29 1.2013246176507124 1103 30-31 1.0286941949997228 1103 32-33 1.6851832528755466 1103 34-35 1.4282728869105412 1103 36-37 1.225038202718828 1103 38-39 1.2391693784564808 1103 40-41 1.1912176315721155 1103 42-43 1.0809791634846206 1103 44-45 0.4273701028419481 1103 46-47 1.3799114898145959 1103 48-49 1.2716579881379957 1103 50-51 1.224161931367295 1103 52-53 1.6630276013748393 1103 54-55 2.4010951520705817 1103 56-57 2.129750814652194 1103 58-59 2.605731344262832 1103 60-61 2.4395820863337114 1103 62-63 2.930914928970491 1103 64-65 2.3708948058092965 1103 66-67 3.1148118005942287 1103 68-69 3.412111658254428 1103 70-71 3.433161813215772 1103 72-73 3.1093896592469186 1103 74-75 2.563186826170597 1103 76-77 2.9875388643048133 1103 78-79 3.557895504151155 1103 80-81 3.1537481506702214 1103 82-83 4.300562434533262 1103 84-85 3.471889753747469 1103 86-87 3.9026206325591204 1103 88-89 4.163172516240429 1103 90-91 4.257749897735753 1103 92-93 3.3995327600619696 1103 94-95 3.508184471274607 1103 96-97 4.1798042848151375 1103 98-99 5.0946061710957835 1103 100-101 4.228019186523245 1104 1 0.6815193132268114 1104 2 0.9751549247541398 1104 3 0.7936352283443426 1104 4 -0.2004871652059066 1104 5 0.7089439533780393 1104 6 0.6785824747497884 1104 7 0.585379577823538 1104 8 0.7291393725859692 1104 9 1.255958396786923 1104 10-11 1.175082037860264 1104 12-13 0.7687232417395364 1104 14-15 1.1259822228450318 1104 16-17 1.337928757544141 1104 18-19 1.1074724367271571 1104 20-21 1.1775925123814588 1104 22-23 0.9005655226370735 1104 24-25 0.6332181808532198 1104 26-27 0.6115202134642459 1104 28-29 0.19630082071787314 1104 30-31 0.9032314768611727 1104 32-33 0.7921372454720554 1104 34-35 1.0315251343986418 1104 36-37 1.1863020842629837 1104 38-39 1.6209779453523012 1104 40-41 1.9059188478597946 1104 42-43 2.2396253824164063 1104 44-45 2.91917338153047 1104 46-47 3.1535762174719224 1104 48-49 3.6664226629132486 1104 50-51 2.3190852523614787 1104 52-53 2.4411873052352284 1104 54-55 2.1473934069621734 1104 56-57 2.5052135327907443 1104 58-59 2.442325738763099 1104 60-61 2.6719987970687704 1104 62-63 2.6497145059139093 1104 64-65 2.6290915271207744 1104 66-67 2.5871809174636127 1104 68-69 2.6416198549757404 1104 70-71 2.224277624955594 1104 72-73 2.2370998654870036 1104 74-75 1.7547521355307225 1104 76-77 1.5558889436279202 1104 78-79 1.7257960858539576 1104 80-81 1.569136833906608 1104 82-83 1.9385846450039104 1104 84-85 2.1864587648527056 1104 86-87 2.7439744136273383 1104 88-89 1.9842989043948442 1104 90-91 1.2771840595548944 1104 92-93 1.4709235585812692 1104 94-95 0.9332241328293414 1104 96-97 0.663014226644858 1104 98-99 0.7738763984886958 1104 100-101 -1.0648152925883352 1105 1 -0.17222044087155197 1105 2 -0.6408799113114334 1105 3 -1.0959651814917208 1105 4 -1.7773314275009895 1105 5 -1.0077568662940948 1105 6 -0.1262330990207019 1105 7 -0.4625200123403985 1105 8 -0.2959630864304259 1105 9 -0.03553750485242091 1105 10-11 -0.43915976541842383 1105 12-13 -0.32515483203095386 1105 14-15 -0.7187923673189047 1105 16-17 -0.7574862014722541 1105 18-19 -0.0025429321253014336 1105 20-21 -0.05895974171689744 1105 22-23 -1.036678329821946 1105 24-25 -0.21603899127792658 1105 26-27 -0.46609249145378584 1105 28-29 -0.3750362694460634 1105 30-31 -0.20089249854866154 1105 32-33 -0.6436977955115495 1105 34-35 -0.752926709863651 1105 36-37 0.3691914285252764 1105 38-39 0.6207217978113206 1105 40-41 0.8600684380237311 1105 42-43 1.239497308645916 1105 44-45 1.169557602841948 1105 46-47 1.60823810271782 1105 48-49 0.8628878268476754 1105 50-51 1.3869643507221348 1105 52-53 2.0077856658909674 1105 54-55 2.6659641036834856 1105 56-57 2.3124625082005785 1105 58-59 2.034410779746704 1105 60-61 1.8637252314949997 1105 62-63 1.9747657354221069 1105 64-65 2.0271448058092965 1105 66-67 1.6284206715619725 1105 68-69 1.1074241582544282 1105 70-71 0.9329097970867402 1105 72-73 1.2409420786017584 1105 74-75 1.346452955202853 1105 76-77 0.6072465255951336 1105 78-79 0.8784600202801869 1105 80-81 0.4478509732508691 1105 82-83 0.12339509582358232 1105 84-85 0.922242576328113 1105 86-87 0.05660248739782858 1105 88-89 -0.09741216117892648 1105 90-91 0.6425785267680091 1105 92-93 -0.31468094961545034 1105 94-95 -0.8801723835641013 1105 96-97 -1.1006896667977664 1105 98-99 -1.0986901998719603 1105 100-101 -2.6668900876703034 1106 1 -0.23472044087155197 1106 2 -0.1877549113114334 1106 3 0.12278481850827916 1106 4 -0.2460814275009895 1106 5 0.03911813370590522 1106 6 0.4518919009792981 1106 7 -0.4937700123403985 1106 8 -0.3428380864304259 1106 9 0.1363374951475791 1106 10-11 -0.11103476541842383 1106 12-13 0.12015766796904614 1106 14-15 0.3749576326810953 1106 16-17 0.1956387985277459 1106 18-19 0.13808206787469857 1106 20-21 -0.05895974171689744 1106 22-23 0.025821670178054035 1106 24-25 0.11208600872207342 1106 26-27 -0.6379674914537858 1106 28-29 -0.10159876944606339 1106 30-31 0.010045001451338464 1106 32-33 -0.026510295511549486 1106 34-35 -1.096676709863651 1106 36-37 -0.4667460714747236 1106 38-39 -0.48084070218867936 1106 40-41 -0.5539940619762689 1106 42-43 -1.041752691354084 1106 44-45 -0.2835673971580519 1106 46-47 0.3348006027178201 1106 48-49 0.6050753268476754 1106 50-51 0.7229018507221348 1106 52-53 1.3749731658909674 1106 54-55 1.5722141036834856 1106 56-57 1.6796500082005785 1106 58-59 0.4172232797467039 1106 60-61 0.3324752314949997 1106 62-63 0.20132823542210687 1106 64-65 0.7146448058092965 1106 66-67 0.34717067156197245 1106 68-69 0.5683616582544282 1106 70-71 0.5579097970867402 1106 72-73 -0.15749542139824158 1106 74-75 -0.07542204479714698 1106 76-77 0.3494340255951336 1106 78-79 0.4956475202801869 1106 80-81 0.7447259732508691 1106 82-83 0.5140200958235823 1106 84-85 0.875367576328113 1106 86-87 0.2831649873978286 1106 88-89 0.4729003388210735 1106 90-91 0.3378910267680091 1106 92-93 -0.21311844961545034 1106 94-95 -1.3879848835641013 1106 96-97 -1.3194396667977664 1106 98-99 -1.3252526998719603 1106 100-101 -0.7606400876703034 1107 1 0.9453276360515268 1107 2 1.3151297040731826 1107 3 1.2052367415852032 1107 4 0.9601685724990077 1107 5 0.6090700567828264 1107 6 0.6117476702100646 1107 7 0.7430088338134482 1107 8 0.7064407597234208 1107 9 0.6322509566860433 1107 10-11 0.7815133115046535 1107 12-13 0.8095807448921235 1107 14-15 0.5143807096041755 1107 16-17 0.8383070677585138 1107 18-19 0.38868302941315847 1107 20-21 0.23370852751386906 1107 22-23 0.5111582086395927 1107 24-25 0.8285523548759244 1107 26-27 1.0403978931616038 1107 28-29 0.7154685382462418 1107 30-31 0.46653538606672385 1107 32-33 0.8609897044884534 1107 34-35 0.4837319439825052 1107 36-37 0.48024912083297266 1107 38-39 -0.16653781757329256 1107 40-41 0.5329049764852698 1107 42-43 -0.042594037507932114 1107 44-45 1.1581393336111745 1107 46-47 1.0248246411793573 1107 48-49 1.6140897499245952 1107 50-51 1.7646085814913697 1107 52-53 1.6414395120448155 1107 54-55 2.0999785267604096 1107 56-57 2.86498654666212 1107 58-59 2.7119348182082454 1107 60-61 3.308316577648842 1107 62-63 2.12260227388364 1107 64-65 2.013202498116989 1107 66-67 2.084911056177358 1107 68-69 0.889034735177507 1107 70-71 0.7981742201636628 1107 72-73 0.9729132324479117 1107 74-75 1.6392414167413136 1107 76-77 1.8412609486720548 1107 78-79 2.2313446356648043 1107 80-81 2.5304230886354837 1107 82-83 2.550438365054351 1107 84-85 1.8985646917127283 1107 86-87 2.1108092181670592 1107 88-89 1.9667705311287662 1107 90-91 1.4722660267680077 1107 92-93 1.5831555888460898 1107 94-95 2.14230357797436 1107 96-97 2.950151679356079 1107 98-99 2.1898915308972704 1107 100-101 2.0678454892527753 1108 1 -1.7055084843498136 1108 2 -1.2683708533404179 1108 3 -2.1700140945351976 1108 4 -1.99925171735606 1108 5 -2.827050344554962 1108 6 -2.33139614249896 1108 7 -2.9582174761085156 1108 8 -2.3715971444014414 1108 9 -2.9626208381857566 1108 10-11 -2.3635257074474083 1108 12-13 -2.494878563915016 1108 14-15 -3.3813829470290457 1108 16-17 -3.2990396435012386 1108 18-19 -2.5076380408209573 1108 20-21 -2.412333835919796 1108 22-23 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1.0650015539149145 2314 68-69 0.7179940111956036 2314 70-71 1.186862002969093 2314 72-73 0.748478843307641 2314 74-75 0.9777029552028544 2314 76-77 0.7946546138304278 2314 78-79 1.4865482555743057 2314 80-81 1.785626708544985 2314 82-83 2.063560537000054 2314 84-85 2.383180076328113 2314 86-87 2.4447458697507685 2314 88-89 2.356815779997543 2314 90-91 1.8016777914738924 2314 92-93 1.6976352268551373 2314 94-95 2.2337062929064864 2314 96-97 2.1098801861434104 2314 98-99 2.488534064833921 2314 100-101 2.5615106476238125 2315 1 -0.008339626918065335 2315 2 -0.6049060741021322 2315 3 -0.2852820419568367 2315 4 -0.5723895670358701 2315 5 -0.5535417500150217 2315 6 -0.3667854246020994 2315 7 -0.34733105885202775 2315 8 -1.7820270949897576E-4 2315 9 -0.45486890020125514 2315 10-11 -0.5779679049533115 2315 12-13 -0.9975748901704904 2315 14-15 -0.7660307394119243 2315 16-17 -0.49603998054201526 2315 18-19 -0.753087990264838 2315 20-21 -0.49482456729828783 2315 22-23 -0.7063730972638069 2315 24-25 -0.9106247470918802 2315 26-27 -0.5093337705235541 2315 28-29 -1.2480377229344342 2315 30-31 -1.5099404636649396 2315 32-33 -1.0890102955115495 2315 34-35 -1.6840676982357436 2315 36-37 -1.7492678738003065 2315 38-39 -1.4523159928863478 2315 40-41 -1.5102077247669712 2315 42-43 -2.6836494936796633 2315 44-45 -2.7528624552975884 2315 46-47 -2.639218292631014 2315 48-49 -2.4427081033848808 2315 50-51 -1.9673234399755408 2315 52-53 -2.0954120085276386 2315 54-55 -2.466121652130468 2315 56-57 -2.7502191778459313 2315 58-59 -1.9904802086253888 2315 60-61 -2.325046570830583 2315 62-63 -1.9623708924848664 2315 64-65 -1.7977098453534914 2315 66-67 -1.879936886577564 2315 68-69 -1.5181208998851083 2315 70-71 -1.5458329354713989 2315 72-73 -1.3262817586075464 2315 74-75 -1.8257854168901702 2315 76-77 -1.3155369046374226 2315 78-79 -1.492179514603535 2315 80-81 -1.5919382709351773 2315 82-83 -1.3397226367345567 2315 84-85 -0.8435641097183968 2315 86-87 -0.850192570741708 2315 88-89 -0.5011185565277643 2315 90-91 -0.8637804848598982 2315 92-93 -0.7808873449642846 2315 94-95 -0.8727232556571245 2315 96-97 -0.8773975156349749 2315 98-99 -0.9455288626626555 2315 100-101 -0.58931014580984 2316 1 -0.7588681681442786 2316 2 -0.20480036585688666 2316 3 -0.006476545128084155 2316 4 -0.3511950638646226 2316 5 -0.1412795935668214 2316 6 -0.08930128083888178 2316 7 -0.05769046688585178 2316 8 0.22392327720594096 2316 9 -0.07246932303424103 2316 10-11 -0.15790976541842383 2316 12-13 0.3531122134235929 2316 14-15 0.0681394508629154 2316 16-17 0.3440762985277459 2316 18-19 0.1260082042383388 2316 20-21 -0.018476787171444187 2316 22-23 -0.11480332982194597 2316 24-25 -0.5391924003688331 2316 26-27 -0.36239930963559885 2316 28-29 0.04754895782666324 2316 30-31 0.1591927287240651 2316 32-33 0.08144424994299726 2316 34-35 -0.7167051189545575 2316 36-37 -0.6336494805656301 2316 38-39 -0.9147895658250391 2316 40-41 -0.4964656528853624 2316 42-43 -0.533940191354084 2316 44-45 -0.22035716988532528 2316 46-47 0.3113631027178201 2316 48-49 0.8160128268476754 2316 50-51 -0.16843337655059187 2316 52-53 0.03051293861824078 2316 54-55 0.774628876410759 2316 56-57 0.8969795536551253 2316 58-59 0.9371096433830637 2316 60-61 1.3587536405859062 2316 62-63 0.33414073542210687 2316 64-65 -0.31660519419070354 2316 66-67 -0.22243160116530092 2316 68-69 0.4064298400726081 2316 70-71 0.08418820617765022 2316 72-73 0.5974761695108484 2316 74-75 0.377702955202853 2316 76-77 0.5170476619587703 2316 78-79 0.6114145657347336 2316 80-81 1.1673112005235957 2316 82-83 1.3357530503690356 2316 84-85 1.4059073490553864 2316 86-87 1.3300399873978286 2316 88-89 1.3983264751847102 2316 90-91 1.5324932994952825 2316 92-93 1.7662849594754597 2316 94-95 2.8080378437086253 2316 96-97 2.7664978332022336 2316 98-99 2.460258663764403 2316 100-101 3.35796786687515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1170.0 3 169.0 4 142.0 5 141.0 6 129.0 7 154.0 8 174.0 9 187.0 10 223.0 11 282.0 12 456.0 13 678.0 14 700.0 15 848.0 16 1104.0 17 1558.0 18 1515.0 19 887.0 20 1094.0 21 1064.0 22 1010.0 23 916.0 24 904.0 25 853.0 26 994.0 27 1021.0 28 1191.0 29 1396.0 30 1550.0 31 1847.0 32 2120.0 33 2640.0 34 3706.0 35 6234.0 36 8338.0 37 12129.0 38 9273.0 39 563.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.90767361814133 18.70679431926648 14.112168455152865 13.27336360743933 2 14.773547325578038 27.86710157896256 31.727910400369485 25.631440695089918 3 31.55661398571119 26.37078732770441 30.751244858194415 11.321353828389984 4 12.610961158181896 18.31382341478905 39.810337610600314 29.264877816428747 5 8.903872253183563 43.07701192573128 32.8997141289596 15.119401692125553 6 23.19313180867181 37.09256186422336 14.447731043936225 25.266575283168603 7 19.214118733585384 39.257987243499095 22.91552425755433 18.612369765361194 8 23.262525975525282 23.484761025167398 19.172823828215193 34.07988917109213 9 19.787593252622617 25.496024588389776 27.467136116361957 27.249246042625646 10-11 25.358737762515243 24.972765509230868 22.913044419274083 26.755452308979805 12-13 33.87684426968724 25.022185346848957 23.976047040150068 17.124923343313732 14-15 25.726551226551226 27.58802308802309 32.58008658008658 14.105339105339104 16-17 16.13496666955242 31.186333073615558 32.22808992006464 20.45061033676738 18-19 15.102208689001293 34.88227781425654 32.07758191487059 17.937931581871577 20-21 20.74604354446457 26.511319107172497 28.705140325176266 24.037497023186667 22-23 16.926363498581832 27.272858493493747 32.88418651982188 22.91659148810254 24-25 24.3814239519575 27.610722947222545 29.264349232012936 18.74350386880702 26-27 20.88737792598688 23.341489645813933 39.1264805872545 16.64465184094469 28-29 15.263693513579838 35.31407207454295 32.237948481064734 17.184285930812482 30-31 17.459836314034554 28.439353916771314 31.009396786905125 23.091412982289008 32-33 18.215210974879305 29.91318529851123 33.99412575502811 17.877477971581353 34-35 21.86464947424753 27.21112541402103 31.39761928867933 19.526605823052112 36-37 18.609451385116785 26.36465688937172 37.87361940974108 17.152272315770414 38-39 18.383348416289593 30.09158371040724 34.483981900452484 17.041085972850677 40-41 16.641331885631562 32.236699239956565 32.89250814332247 18.229460731089393 42-43 15.669507418053858 32.220926949004046 27.969212723283782 24.140352909658315 44-45 19.263410960491253 26.586577453365773 30.38972888425443 23.760282701888542 46-47 22.754100735054493 31.22352174385342 31.130101024731143 14.892276496360937 48-49 19.785019448206924 28.822459962769543 32.5534735149465 18.839047074077026 50-51 12.926352378883339 31.32522268086031 37.56970091969006 18.178724020566296 52-53 17.656474064632008 33.80143980763938 27.914741370569406 20.627344757159204 54-55 14.579381055131565 28.07046761183439 31.423689050439517 25.92646228259453 56-57 20.883042798401576 26.027972432964614 29.360195749116812 23.728789019516995 58-59 25.55949623624783 25.54791546033584 29.17486971627099 19.717718587145338 60-61 19.221335494280485 28.213383884061326 34.807649174088894 17.757631447569295 62-63 16.17768445442039 28.530157121135325 33.99391789153573 21.29824053290855 64-65 15.557551351507954 34.04916417996929 29.86933974563259 20.52394472289017 66-67 19.08018167462278 27.388825706814245 26.982448261124674 26.548544357438303 68-69 16.166775338694485 27.570817938129395 31.116423965804536 25.145982757371588 70-71 23.114758848499775 27.166306883212844 27.829129420627908 21.889804847659473 72-73 20.198464435752573 22.98348544111256 36.44719687092569 20.370853252209184 74-75 16.3072375998783 32.411639851350664 30.124671298073793 21.15645125069725 76-77 18.30677867902665 25.700318655851678 29.987688296639632 26.005214368482036 78-79 19.795113122171944 26.232760180995474 32.49085972850679 21.48126696832579 80-81 22.6631623151889 24.767945320532313 30.408937544347424 22.15995481993136 82-83 19.301485230967536 23.911422012701586 34.978601377332666 21.808491378998212 84-85 19.09524085392347 27.46067838832083 32.02864756828781 21.41543318946789 86-87 19.11451619911075 28.28001216562631 30.50385968977653 22.101611945486408 88-89 19.011508738384432 27.84260043891098 26.76487843035004 26.381012392354545 90-91 21.784107620199798 23.531414579726313 28.416195188386062 26.26828261168783 92-93 24.80984048333116 26.67376595528897 28.945538314426045 19.570855246953826 94-95 22.508928377389655 25.037850524836465 29.724071484972075 22.72914961280181 96-97 18.44924628211722 26.113191692804726 32.68114944693551 22.756412578142545 98-99 22.517837093911847 27.94755713302669 25.792618883778207 23.741986889283258 100-101 21.646774240273473 23.739100205683826 27.96286103305426 26.651264520988445 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1163.0 1 689.5 2 248.5 3 259.0 4 197.5 5 180.5 6 276.0 7 431.5 8 570.5 9 616.0 10 478.0 11 305.5 12 248.5 13 217.5 14 219.0 15 263.0 16 302.0 17 311.5 18 302.0 19 307.0 20 324.5 21 367.0 22 400.0 23 425.5 24 478.5 25 527.5 26 593.5 27 663.0 28 723.5 29 761.5 30 785.0 31 833.0 32 854.0 33 881.0 34 862.0 35 756.5 36 718.0 37 701.5 38 610.5 39 501.0 40 427.0 41 393.0 42 417.0 43 451.5 44 453.5 45 589.5 46 1727.0 47 5609.5 48 12422.0 49 12242.0 50 5971.0 51 2834.0 52 1489.0 53 957.5 54 676.5 55 464.0 56 348.0 57 291.5 58 254.5 59 220.0 60 165.0 61 150.0 62 139.0 63 108.5 64 95.5 65 88.5 66 74.0 67 72.0 68 69.5 69 56.0 70 47.0 71 45.5 72 39.0 73 34.0 74 29.5 75 27.0 76 28.5 77 19.0 78 14.0 79 15.0 80 14.0 81 9.5 82 3.5 83 2.5 84 1.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3085351787773933 2 0.10668973471741638 3 0.10813148788927336 4 0.11389850057670127 5 0.14129181084198386 6 0.07929642445213379 7 0.08938869665513263 8 0.0922722029988466 9 0.0850634371395617 10-11 0.07857554786620531 12-13 0.08290080738177624 14-15 0.08650519031141869 16-17 0.07785467128027682 18-19 0.09443483275663206 20-21 0.10741061130334487 22-23 0.11606113033448674 24-25 0.12687427912341406 26-27 0.12759515570934257 28-29 0.12182814302191464 30-31 0.11678200692041521 32-33 0.10741061130334487 34-35 0.31862745098039214 36-37 0.4649653979238754 38-39 0.428921568627451 40-41 0.4108996539792387 42-43 0.4404555940023068 44-45 0.4498269896193772 46-47 0.45775663206459055 48-49 0.47649942329873124 50-51 0.4541522491349481 52-53 0.46568627450980393 54-55 0.44261822376009224 56-57 0.4209919261822376 58-59 0.40369088811995385 60-61 0.36692618223760093 62-63 0.4397347174163784 64-65 0.4700115340253748 66-67 0.4837081891580161 68-69 0.49740484429065746 70-71 0.48587081891580164 72-73 0.4757785467128028 74-75 0.48803344867358706 76-77 0.461361014994233 78-79 0.428921568627451 80-81 0.4368512110726644 82-83 0.45198961937716264 84-85 0.4527104959630911 86-87 0.45126874279123413 88-89 0.46929065743944637 90-91 0.4894752018454441 92-93 0.4887543252595156 94-95 0.48803344867358706 96-97 0.4837081891580161 98-99 0.4793829296424452 100-101 0.46424452133794697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 69360.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.752595155709344 #Duplication Level Percentage of deduplicated Percentage of total 1 82.40506329113924 25.341695501730104 2 8.949835911861229 5.504613610149942 3 3.5114861697140176 3.2396193771626294 4 1.640881387716831 2.0184544405997693 5 0.8813877168307548 1.3552479815455594 6 0.46882325363338023 0.8650519031141869 7 0.30473511486169713 0.655997693194925 8 0.2156586966713549 0.530565167243368 9 0.19690576652601968 0.5449826989619377 >10 1.0595405532114393 6.172145328719723 >50 0.12658227848101267 2.840253748558247 >100 0.13127051101734646 8.88840830449827 >500 0.04688232536333802 10.628604382929643 >1k 0.06094702297233943 31.414359861591695 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4463 6.434544405997693 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3088 4.4521337946943484 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1941 2.7984429065743943 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1464 2.110726643598616 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1450 2.0905420991926182 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1398 2.0155709342560555 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1236 1.782006920415225 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1220 1.7589388696655135 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1178 1.69838523644752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1133 1.633506343713956 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1108 1.5974625144175316 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1094 1.5772779700115342 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1016 1.4648212226066897 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1000 1.441753171856978 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 864 1.245674740484429 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 809 1.1663783160322954 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 763 1.1000576701268743 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 748 1.0784313725490196 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 731 1.053921568627451 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 718 1.0351787773933103 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 706 1.0178777393310265 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 531 0.7655709342560554 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 502 0.7237600922722031 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 467 0.6732987312572087 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 456 0.657439446366782 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 420 0.6055363321799307 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 382 0.5507497116493656 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 323 0.46568627450980393 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 321 0.46280276816608995 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 270 0.38927335640138405 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 269 0.38783160322952714 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 251 0.36188004613610153 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 236 0.3402537485582468 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 227 0.327277970011534 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 218 0.31430219146482125 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 207 0.2984429065743945 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 198 0.28546712802768165 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 184 0.265282583621684 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 178 0.2566320645905421 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 163 0.2350057670126874 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 161 0.23212226066897348 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 153 0.22058823529411764 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.21914648212226065 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 0.18021914648212226 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 125 0.18021914648212226 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 120 0.17301038062283738 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 118 0.1701268742791234 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 116 0.16724336793540945 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 114 0.1643598615916955 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 107 0.15426758938869664 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 104 0.14994232987312572 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 99 0.14273356401384082 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 0.13985005767012687 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 92 0.132641291810842 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 90 0.12975778546712802 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 88 0.12687427912341406 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 87 0.12543252595155707 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 82 0.1182237600922722 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 82 0.1182237600922722 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 81 0.11678200692041521 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 81 0.11678200692041521 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 77 0.11101499423298732 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 75 0.10813148788927336 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 73 0.1052479815455594 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 73 0.1052479815455594 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 73 0.1052479815455594 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 70 0.10092272202998846 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.208765859284891E-4 0.0 20-21 0.0 0.0 0.0 0.004325259515570935 0.0 22-23 0.0 0.0 0.0 0.01513840830449827 0.0 24-25 0.0 0.0 0.0 0.022347174163783158 0.0 26-27 0.0 0.0 0.0 0.054065743944636674 0.0 28-29 0.0 0.0 0.0 0.12254901960784315 0.0 30-31 0.0 0.0 0.0 0.25086505190311414 0.0 32-33 0.0 0.0 0.0 0.5327277970011535 0.0 34-35 0.0 0.0 0.0 0.9479527104959631 0.0 36-37 0.0 0.0 0.0 1.4763552479815456 0.0 38-39 0.0 0.0 0.0 2.0963091118800463 0.0 40-41 0.0 0.0 0.0 2.706170703575548 0.0 42-43 0.0 0.0 0.0 3.2547577854671284 0.0 44-45 0.0 0.0 0.0 3.847318339100346 0.0 46-47 0.0 0.0 0.0 4.53215109573241 0.0 48-49 0.0 0.0 0.0 5.135524798154556 0.0 50-51 0.0 0.0 0.0 5.726643598615917 0.0 52-53 0.0 0.0 0.0 6.182958477508651 0.0 54-55 0.0 0.0 0.0 6.749567474048443 0.0 56-57 0.0 0.0 0.0 7.6023644752018456 0.0 58-59 0.0 0.0 0.0 8.161043829296425 0.0 60-61 0.0 0.0 0.0 8.81632064590542 0.0 62-63 0.0 0.0 0.0 9.388696655132641 0.0 64-65 0.0 0.0 0.0 9.95242214532872 0.0 66-67 0.0 0.0 0.0 10.649509803921568 0.0 68-69 0.0 0.0 0.0 11.495098039215687 0.0 70-71 0.0 0.0 0.0 12.350778546712803 0.0 72-73 0.0 0.0 0.0 12.995963091118801 0.0 74-75 0.0 0.0 0.0 13.827133794694348 0.0 76-77 0.0 0.0 0.0 14.527104959630911 0.0 78-79 0.0 0.0 0.0 15.897491349480969 0.0 80-81 0.0 0.0 0.0 17.00331603229527 0.0 82-83 0.0 0.0 0.0 17.90801614763552 0.0 84-85 0.0 0.0 0.0 18.90138408304498 0.0 86-87 0.0 0.0 0.0 19.94088811995386 0.0 88-89 0.0 0.0 0.0 21.033737024221452 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 30 1.3470526E-6 79.23249 1 ACCCATG 30 1.3704794E-6 79.00349 3 TACCCAT 30 1.3704794E-6 79.00349 2 ATGGGCA 30 1.3059021E-4 63.20279 5 CTTGGTA 25 0.0016331077 37.8943 16-17 TTGGTAT 25 0.0016331077 37.8943 16-17 CAAATCT 30 0.003923028 31.692995 88-89 TGCTTGG 30 0.003993149 31.57858 14-15 GCTTGGT 35 0.00848101 27.067354 14-15 GTCGCCG 55 9.318374E-5 25.949436 92-93 CATGGGC 185 8.853021E-9 25.622753 4 CGGTGGT 85 1.0990698E-7 25.167965 88-89 CATGGGG 135 1.340428E-5 24.578865 4 GGTCGCC 50 0.0016314583 23.786985 92-93 GAGTACT 695 0.0 23.172771 12-13 GCTTGAT 145 2.3567089E-5 22.85075 9 GGTGGTC 85 3.0934316E-6 22.371525 88-89 GTACTTT 685 0.0 21.782307 14-15 AAGCAGG 55 0.002838934 21.624529 94-95 AGTACTT 620 0.0 21.391941 12-13 >>END_MODULE