##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939109_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69360 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15405132641292 33.0 31.0 34.0 30.0 34.0 2 32.31189446366782 34.0 31.0 34.0 30.0 34.0 3 32.364561707035755 34.0 31.0 34.0 30.0 34.0 4 35.852191464821225 37.0 35.0 37.0 35.0 37.0 5 35.843886966551324 37.0 35.0 37.0 35.0 37.0 6 35.99283448673587 37.0 35.0 37.0 35.0 37.0 7 35.96983852364475 37.0 35.0 37.0 35.0 37.0 8 36.02566320645906 37.0 35.0 37.0 35.0 37.0 9 37.582554786620534 39.0 37.0 39.0 35.0 39.0 10-11 37.53817762399078 39.0 37.0 39.0 34.5 39.0 12-13 37.5347029988466 39.0 37.0 39.0 35.0 39.0 14-15 38.48117791234141 40.0 38.0 41.0 34.0 41.0 16-17 38.51066897347174 40.0 38.0 41.0 34.5 41.0 18-19 38.64016724336794 40.0 38.0 41.0 34.0 41.0 20-21 38.772455305651675 40.0 38.0 41.0 35.0 41.0 22-23 38.71356689734718 40.0 38.0 41.0 35.0 41.0 24-25 38.5121107266436 40.0 38.0 41.0 34.0 41.0 26-27 38.17866926182238 40.0 37.0 41.0 34.0 41.0 28-29 37.98578431372549 40.0 37.0 41.0 33.5 41.0 30-31 37.774142156862744 40.0 36.5 41.0 33.0 41.0 32-33 37.299336793540945 39.0 35.0 41.0 33.0 41.0 34-35 36.96815888119954 39.0 35.0 41.0 33.0 41.0 36-37 36.69673442906574 39.0 35.0 41.0 32.0 41.0 38-39 36.42440888119954 39.0 35.0 41.0 31.0 41.0 40-41 36.29424740484429 39.0 35.0 41.0 31.0 41.0 42-43 35.895400807381776 39.0 35.0 41.0 30.0 41.0 44-45 35.45110294117647 39.0 35.0 41.0 29.0 41.0 46-47 34.862067474048445 38.5 35.0 41.0 24.0 41.0 48-49 34.2224336793541 38.0 35.0 40.5 21.0 41.0 50-51 33.72267156862745 38.0 34.0 40.0 11.5 41.0 52-53 33.51635668973472 38.0 34.0 40.0 2.0 41.0 54-55 33.21238465974625 38.0 34.0 40.0 2.0 41.0 56-57 32.595588235294116 38.0 33.0 40.0 2.0 41.0 58-59 32.13596453287197 38.0 33.0 40.0 2.0 41.0 60-61 31.245004325259515 36.0 31.0 39.5 2.0 41.0 62-63 30.829260380622838 36.0 31.0 39.0 2.0 41.0 64-65 30.282518742791233 36.0 30.5 39.0 2.0 40.5 66-67 29.54524221453287 35.0 29.0 38.5 2.0 40.0 68-69 28.85574538638985 35.0 28.5 37.0 2.0 39.0 70-71 28.110279700115342 34.5 27.0 36.5 2.0 38.5 72-73 27.529664071510958 34.0 26.0 36.0 2.0 37.0 74-75 27.1090830449827 34.0 26.0 35.0 2.0 37.0 76-77 25.375180219146483 31.5 22.0 34.0 2.0 35.5 78-79 26.241608996539792 33.5 24.5 35.0 2.0 36.0 80-81 26.16569348327566 34.0 23.5 35.0 2.0 36.0 82-83 25.894312283737023 33.5 22.0 35.0 2.0 35.0 84-85 25.57045126874279 33.0 20.0 35.0 2.0 35.0 86-87 25.294514129181085 33.0 17.5 35.0 2.0 35.0 88-89 24.762435121107266 33.0 7.0 35.0 2.0 35.0 90-91 24.318576989619377 32.0 2.0 35.0 2.0 35.0 92-93 24.090462802768165 32.0 2.0 35.0 2.0 35.0 94-95 23.786526816608998 32.0 2.0 35.0 2.0 35.0 96-97 23.259349769319492 32.0 2.0 34.0 2.0 35.0 98-99 22.65268886966551 32.0 2.0 34.0 2.0 35.0 100-101 19.607115051903115 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.525718890909765 1101 2 0.5528554515248274 1101 3 0.566865058550654 1101 4 0.40959689200745686 1101 5 0.4242889405025281 1101 6 0.6845857353906624 1101 7 0.4592353636079878 1101 8 0.48653456340890955 1101 9 0.5348337848949569 1101 10-11 0.693278174113324 1101 12-13 0.6696161874682147 1101 14-15 0.8389598582830544 1101 16-17 0.619922763533566 1101 18-19 0.85620232922561 1101 20-21 0.5991535783556898 1101 22-23 0.9480611770592375 1101 24-25 0.9724816852044214 1101 26-27 0.5393649820964868 1101 28-29 0.6364036300106477 1101 30-31 0.34960890580216386 1101 32-33 0.4944419319835802 1101 34-35 0.49755211371882524 1101 36-37 0.9747159511397356 1101 38-39 1.2645709157751313 1101 40-41 1.3615087160182853 1101 42-43 1.0019244000108927 1101 44-45 1.4924703512676416 1101 46-47 0.8393240752967301 1101 48-49 1.5227821239126555 1101 50-51 1.4370622073739483 1101 52-53 1.3598217733966393 1101 54-55 1.5883688645541199 1101 56-57 2.114257233919602 1101 58-59 2.273508339915381 1101 60-61 2.514340550517172 1101 62-63 1.7186350284252114 1101 64-65 1.9031157644256886 1101 66-67 2.538439274248283 1101 68-69 2.0593086375107 1101 70-71 2.854772933939497 1101 72-73 1.8724559635403573 1101 74-75 1.3248444671655406 1101 76-77 2.3108082232181175 1101 78-79 2.0915167439177083 1101 80-81 2.480074547095164 1101 82-83 2.653012226827162 1101 84-85 2.793437291340908 1101 86-87 2.9470027473279465 1101 88-89 3.381122472351965 1101 90-91 3.2891616148800544 1101 92-93 2.4635000554516395 1101 94-95 3.042171161983987 1101 96-97 3.5846496082668544 1101 98-99 3.4411175126536477 1101 100-101 2.887438527561539 1102 1 0.2512031524109801 1102 2 0.3040661052778475 1102 3 0.14676820625040676 1102 4 0.310323284259276 1102 5 0.3767707806962264 1102 6 0.42974312037855356 1102 7 0.4204944435111315 1102 8 0.10427064089074634 1102 9 0.7176425016019792 1102 10-11 0.32508810147410117 1102 12-13 0.37285468625755414 1102 14-15 1.042501020510663 1102 16-17 0.8169566618386526 1102 18-19 0.5105606827365037 1102 20-21 0.3954610844089643 1102 22-23 -0.027120421003722583 1102 24-25 0.42208822273468627 1102 26-27 0.03966764553474178 1102 28-29 -0.19062421502567162 1102 30-31 -0.05505211114698483 1102 32-33 -0.5299224747960807 1102 34-35 -0.9640096296225806 1102 36-37 -1.0320939762210344 1102 38-39 -1.1476566871304428 1102 40-41 -1.2736305091633184 1102 42-43 -1.483547754952788 1102 44-45 -1.054139818223888 1102 46-47 -1.8324374259139233 1102 48-49 -1.8074236872253593 1102 50-51 -0.8613639427471185 1102 52-53 -0.8397847641336256 1102 54-55 -0.5741614017897021 1102 56-57 -0.3394352600271233 1102 58-59 -1.0415642993097975 1102 60-61 -1.591894316310917 1102 62-63 -2.2946821628580807 1102 64-65 -1.743676003128794 1102 66-67 -1.9093512826524446 1102 68-69 -2.2009819193900277 1102 70-71 -2.1303965575859287 1102 72-73 -1.8289665546194485 1102 74-75 -1.6674376151589136 1102 76-77 -1.5322607840458033 1102 78-79 -1.519258074484231 1102 80-81 -1.2638721841397036 1102 82-83 -1.0886645286207788 1102 84-85 -0.8468472122910526 1102 86-87 -0.6472769136890015 1102 88-89 -1.1595857600935524 1102 90-91 -1.329028457759172 1102 92-93 -0.29164522299872075 1102 94-95 -0.03924530290705164 1102 96-97 0.12323919664457605 1102 98-99 0.025863275365509253 1102 100-101 0.30133077937751906 1103 1 -0.15803686945889694 1103 2 0.6438692764096174 1103 3 0.44474524288244766 1103 4 -0.18256900661005204 1103 5 0.36610921700022203 1103 6 0.2963368874643919 1103 7 0.5306639350365572 1103 8 0.5977096786162832 1103 9 0.38276005217606723 1103 10-11 0.4683357777999575 1103 12-13 0.620941072260841 1103 14-15 0.9602156186517234 1103 16-17 0.9223421183722778 1103 18-19 1.0532069375205353 1103 20-21 0.7759968963280457 1103 22-23 0.8985220065523194 1103 24-25 0.8765715469555744 1103 26-27 0.3867152125112341 1103 28-29 0.6752861184899146 1103 30-31 0.8455766477376514 1103 32-33 1.0448451577900286 1103 34-35 1.0949023441335655 1103 36-37 1.2036906055176146 1103 38-39 1.4800087037935654 1103 40-41 1.3865663197049187 1103 42-43 1.7231225566929211 1103 44-45 1.1940832544934494 1103 46-47 1.335003798799029 1103 48-49 1.684648483359659 1103 50-51 0.6674769539177277 1103 52-53 1.4637964277745183 1103 54-55 1.5935531963513583 1103 56-57 1.6620682938274456 1103 58-59 1.8109507362287474 1103 60-61 1.707312900747585 1103 62-63 2.0239345675957168 1103 64-65 2.4382540132736175 1103 66-67 2.484579827243678 1103 68-69 2.7977879001835113 1103 70-71 2.649127772649173 1103 72-73 2.674587299945884 1103 74-75 3.1863076008060958 1103 76-77 3.069160757780331 1103 78-79 3.1468162830882136 1103 80-81 2.9512727037771924 1103 82-83 2.8543371116197918 1103 84-85 2.2603151715252423 1103 86-87 2.662728553779562 1103 88-89 2.3681616428588796 1103 90-91 2.314219218566688 1103 92-93 2.308546138400949 1103 94-95 2.1291527288042644 1103 96-97 1.36777172808252 1103 98-99 1.2314400932988079 1103 100-101 0.8767818455338876 1104 1 0.21980554196363045 1104 2 0.15180158735620353 1104 3 -0.32891948477487176 1104 4 -0.36792067239534987 1104 5 -0.3025845957972422 1104 6 -0.2890676603938829 1104 7 0.12902459077426442 1104 8 -0.326404546661351 1104 9 0.15192980363032405 1104 10-11 -0.40844899216302366 1104 12-13 -0.2951086368877611 1104 14-15 -0.2045120386724406 1104 16-17 -0.15261822007299486 1104 18-19 -0.3785751883387931 1104 20-21 0.28445802800440845 1104 22-23 0.4498176173402655 1104 24-25 0.11255779761659568 1104 26-27 0.05897856523466061 1104 28-29 0.538189344296363 1104 30-31 0.20001874186773705 1104 32-33 0.9852206205081657 1104 34-35 0.6032312706274894 1104 36-37 0.02931196987510276 1104 38-39 0.20660838650112368 1104 40-41 1.0179782710534155 1104 42-43 0.6073108168727188 1104 44-45 0.5949293676610807 1104 46-47 0.9914025296292834 1104 48-49 0.7555104611491856 1104 50-51 1.3767577577252368 1104 52-53 1.83859812000086 1104 54-55 1.6624320964042383 1104 56-57 2.3364469294699575 1104 58-59 2.2000305881589455 1104 60-61 1.8594810657396543 1104 62-63 2.5441619605095127 1104 64-65 2.5708558112640922 1104 66-67 2.7127659721405593 1104 68-69 3.2490041878619884 1104 70-71 2.3568221142673664 1104 72-73 2.3483049096761874 1104 74-75 3.443843296205351 1104 76-77 3.0993035393773702 1104 78-79 2.314584659608574 1104 80-81 2.163329816416006 1104 82-83 1.9956644516515212 1104 84-85 2.2476234740107017 1104 86-87 2.2840929112623733 1104 88-89 2.2553906222348736 1104 90-91 2.19972953057092 1104 92-93 1.9292494699715483 1104 94-95 2.2415271338809397 1104 96-97 1.2206273600232933 1104 98-99 0.8181666929815172 1104 100-101 0.5655708460627089 1105 1 0.012326033766910882 1105 2 0.3006233086676815 1105 3 0.23204362997922345 1105 4 0.01450760629317216 1105 5 0.2501817976453822 1105 6 0.16226430681923176 1105 7 0.17128893503655718 1105 8 0.29680242055176365 1105 9 0.15090521346638752 1105 10-11 0.21001924554189344 1105 12-13 0.10153583032535352 1105 14-15 -0.19452228457408438 1105 16-17 -0.294139736466434 1105 18-19 0.17316661493989471 1105 20-21 -0.037007135930018364 1105 22-23 0.17573974848780693 1105 24-25 0.11422275663299075 1105 26-27 0.0036506963822020566 1105 28-29 -0.500873155703637 1105 30-31 -0.33007859419783614 1105 32-33 -0.20868306801641978 1105 34-35 -0.21003717199546657 1105 36-37 -0.36233762028883376 1105 38-39 -0.10038444136772995 1105 40-41 -0.30925021255314533 1105 42-43 0.35795118572517737 1105 44-45 0.3909078512676416 1105 46-47 1.029056218153869 1105 48-49 0.7292553381983709 1105 50-51 0.5654104216596636 1105 52-53 0.76495570196807 1105 54-55 1.1263152931255505 1105 56-57 1.173409019633894 1105 58-59 1.1942672684868114 1105 60-61 0.7141173362314568 1105 62-63 0.25100109985378083 1105 64-65 1.037044335854258 1105 66-67 -0.7550875114660016 1105 68-69 -1.2185931482035848 1105 70-71 -0.26241456606050306 1105 72-73 -0.4143743936025004 1105 74-75 -0.8280573185487441 1105 76-77 -0.18361141963902483 1105 78-79 -0.04799218465372235 1105 80-81 0.39860133280944865 1105 82-83 0.20211936968430777 1105 84-85 0.5188837199123384 1105 86-87 -0.03737225267205346 1105 88-89 0.23156890092339566 1105 90-91 0.7121526863086238 1105 92-93 0.7112679125944972 1105 94-95 0.1571265191268445 1105 96-97 0.7185781796954238 1105 98-99 1.3942425126536477 1105 100-101 1.4075278132758235 1106 1 0.4185760337669109 1106 2 0.3787483086676815 1106 3 0.5132936299792235 1106 4 0.23325760629317216 1106 5 0.2970567976453822 1106 6 -0.16586069318076824 1106 7 0.3275389350365572 1106 8 -0.37507257944823635 1106 9 0.22903021346638752 1106 10-11 0.03033174554189344 1106 12-13 0.3359108303253535 1106 14-15 0.18829021542591562 1106 16-17 0.471485263533566 1106 18-19 0.2981666149398947 1106 20-21 0.26768036406998164 1106 22-23 0.15230224848780693 1106 24-25 0.48922275663299075 1106 26-27 0.9255256963822021 1106 28-29 0.608501844296363 1106 30-31 0.7324214058021639 1106 32-33 0.9944419319835802 1106 34-35 0.5399628280045334 1106 36-37 0.37203737971116624 1106 38-39 0.96211555863227 1106 40-41 1.1204372874468547 1106 42-43 1.1313886857251774 1106 44-45 0.9534078512676416 1106 46-47 0.6618687181538689 1106 48-49 1.1745678381983709 1106 50-51 1.3232229216596636 1106 52-53 1.47589320196807 1106 54-55 1.6497527931255505 1106 56-57 1.212471519633894 1106 58-59 1.9989547684868114 1106 60-61 2.003179836231457 1106 62-63 0.6728760998537808 1106 64-65 1.380794335854258 1106 66-67 0.3386624885339984 1106 68-69 1.2345318517964152 1106 70-71 0.8703979339394969 1106 72-73 1.5543756063974996 1106 74-75 1.546942681451256 1106 76-77 1.4179510803609752 1106 78-79 1.3582578153462777 1106 80-81 1.3986013328094486 1106 82-83 1.4677443696843078 1106 84-85 2.1048212199123384 1106 86-87 1.5563777473279465 1106 88-89 2.2706314009233957 1106 90-91 2.704340186308624 1106 92-93 2.062830412594497 1106 94-95 2.8680640191268445 1106 96-97 1.7420156796954238 1106 98-99 1.4645550126536477 1106 100-101 1.9856528132758235 1107 1 0.239729879920759 1107 2 0.15230600097537206 1107 3 -0.26939867771308457 1107 4 -0.2761173937068264 1107 5 0.04873948995307131 1107 6 0.23678353758846526 1107 7 -0.6924129880403669 1107 8 -5.533486790056941E-4 1107 9 -0.01351786345669126 1107 10-11 0.22912982246497648 1107 12-13 0.4230502534022733 1107 14-15 0.6009104077336076 1107 16-17 0.8179996866104915 1107 18-19 0.9842243072475867 1107 20-21 0.5752524794545977 1107 22-23 0.031028210026271097 1107 24-25 0.2583333335560667 1107 26-27 -0.31365699592549134 1107 28-29 0.14588165198866676 1107 30-31 0.33999352118677706 1107 32-33 1.1280957781374283 1107 34-35 0.7791455203122268 1107 36-37 1.1638643027880917 1107 38-39 0.7805049817091927 1107 40-41 0.7396680566776226 1107 42-43 0.45686945495594244 1107 44-45 0.17323958203687084 1107 46-47 0.29468121815386894 1107 48-49 1.1159139920445256 1107 50-51 1.262886383198122 1107 52-53 0.9085855096603765 1107 54-55 0.5765556777409344 1107 56-57 1.4788176734800516 1107 58-59 0.9001566915637369 1107 60-61 1.094285605462229 1107 62-63 1.2900635998537808 1107 64-65 0.9726212589311807 1107 66-67 1.4498403731493852 1107 68-69 1.5351328133348794 1107 70-71 1.2336190877856517 1107 72-73 0.5393515679359595 1107 74-75 0.5227840276051019 1107 76-77 0.4612203111302051 1107 78-79 0.3437145461155069 1107 80-81 0.40809652511714134 1107 82-83 -0.30509216877723233 1107 84-85 -0.5962605108568937 1107 86-87 0.2902719780971772 1107 88-89 0.6625785163080131 1107 90-91 -0.08652519830676297 1107 92-93 -0.577434010482424 1107 94-95 -0.2795321347193074 1107 96-97 0.6205012566184998 1107 98-99 -1.3511901796540435 1107 100-101 -2.4778087251857137 1108 1 -0.01801816913163634 1108 2 0.09478091736333027 1108 3 0.044090731428497065 1108 4 0.08040434542360941 1108 5 0.19991005851494492 1108 6 -0.1968842439054086 1108 7 -0.27368389105039626 1108 8 0.07012669591408383 1108 9 -0.37717449667854197 1108 10-11 -0.19996716750158328 1108 12-13 -0.5145194232978341 1108 14-15 0.14628387484620475 1108 16-17 0.21956044469298774 1108 18-19 0.3919166149398947 1108 20-21 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60-61 1.5898275635041834 2316 62-63 0.7673363271265075 2316 64-65 0.6336352449451645 2316 66-67 0.20158862489763507 2316 68-69 0.38723071543277854 2316 70-71 0.20704566121222356 2316 72-73 1.0153131063974996 2316 74-75 0.6357210905421624 2316 76-77 0.4009056258155219 2316 78-79 0.7424907698917309 2316 80-81 0.816925196445812 2316 82-83 0.5970057333206746 2316 84-85 0.4151905380941585 2316 86-87 0.5890482018733998 2316 88-89 -0.38348791725842446 2316 90-91 0.15604473176317057 2316 92-93 0.3717792762308605 2316 94-95 0.0243140191268445 2316 96-97 0.9913054524226972 2316 98-99 0.16839023992637436 2316 100-101 -0.0938926412696297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 5.0 5 7.0 6 15.0 7 35.0 8 48.0 9 81.0 10 148.0 11 259.0 12 411.0 13 547.0 14 627.0 15 884.0 16 1103.0 17 1553.0 18 1522.0 19 1031.0 20 1093.0 21 1110.0 22 1065.0 23 993.0 24 1045.0 25 1038.0 26 1032.0 27 976.0 28 1098.0 29 1345.0 30 1585.0 31 1879.0 32 2279.0 33 2791.0 34 3751.0 35 6446.0 36 8678.0 37 14383.0 38 8245.0 39 251.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.80260303687636 19.350686912509037 14.456977584960232 13.389732465654374 2 14.24163783160323 28.885524798154556 31.829584775086506 25.043252595155707 3 31.601787773933104 26.3941753171857 31.16926182237601 10.83477508650519 4 12.45242214532872 19.152249134948097 38.84659746251442 29.548731257208765 5 8.92156862745098 44.24884659746252 32.33419838523645 14.495386389850056 6 23.117070357554788 37.99019607843137 13.861014994232987 25.031718569780853 7 19.55017301038062 39.36707035755479 22.01845444059977 19.064302191464822 8 23.95040369088812 23.718281430219147 18.647635524798154 33.68367935409458 9 18.9777970011534 25.70790080738178 26.696943483275664 28.617358708189155 10-11 25.52047289504037 25.275374855824683 22.836649365628606 26.367502883506344 12-13 34.1219723183391 24.731113033448672 23.75360438292964 17.393310265282583 14-15 25.01676050144537 28.136736856522898 32.206114519279986 14.64038812275175 16-17 16.276303553277536 31.271581709523705 32.000403699618644 20.451711037580107 18-19 15.378460207612457 34.7873414071511 31.60899653979239 18.22520184544406 20-21 20.8628892733564 26.615484429065745 28.212946943483274 24.30867935409458 22-23 16.78056516724337 27.831603229527108 32.37673010380623 23.011101499423297 24-25 24.34778220719584 28.306864957215666 29.079650228159082 18.265702607429407 26-27 21.362764745743164 23.70925186349284 38.580429360284896 16.347554030479102 28-29 15.345299884659747 35.645184544406 31.982410611303347 17.02710495963091 30-31 17.81612924154934 28.34908482738237 30.7965137654361 23.038272165632186 32-33 18.004613610149942 30.00576701268743 34.38581314878893 17.603806228373703 34-35 21.61168464155949 26.97603668031605 32.070765326720114 19.34151335140435 36-37 18.450123303673152 26.617008696153793 37.836200804718715 17.096667195454348 38-39 17.90606794716502 30.082338351502568 34.78470900386456 17.226884697467842 40-41 16.497483886782113 31.960405461912245 32.672703415858 18.86940723544764 42-43 15.663953513521308 32.21538927379296 28.508294462427997 23.612362750257738 44-45 19.500994838374808 26.332218345396353 30.497563366879092 23.66922344934975 46-47 22.937902795599708 31.42490520336222 30.769618939142724 14.867573061895357 48-49 19.95314302191465 28.741349480968857 32.53243944636679 18.77306805074971 50-51 12.711216839677048 31.580882352941174 37.41565743944636 18.29224336793541 52-53 17.512975778546714 34.14504036908881 27.94405997693195 20.397923875432525 54-55 14.50980392156863 28.375144175317185 30.840542099192618 26.27450980392157 56-57 20.914071510957324 26.712081891580162 28.74639561707036 23.627450980392155 58-59 25.45381143936445 26.305924420029704 28.638781954236777 19.601482186369076 60-61 18.555980102371855 29.981255857544518 33.583014923221114 17.87974911686252 62-63 15.375299172409814 29.868219960206467 32.818852908100006 21.937627959283716 64-65 14.70104816966796 35.55486670799752 28.777808215228017 20.966276907106504 66-67 18.806770668127683 28.068860117073736 25.997721964301164 27.12664725049742 68-69 15.851205709548355 28.385538694445444 29.72785927981833 26.03539631618787 70-71 22.229671280276815 28.00965974625144 26.767589388696656 22.993079584775085 72-73 19.75994809688581 22.964965397923876 34.98702422145329 22.288062283737023 74-75 15.1138985005767 32.42214532871973 28.737745098039213 23.72621107266436 76-77 17.389545467679948 25.1842112867339 29.2614210997775 28.16482214580865 78-79 18.356401384083046 26.1310553633218 31.83246828143022 23.680074971164935 80-81 21.098807618553284 24.21601280332194 29.864325158239254 24.820854419885517 82-83 17.182814302191467 23.848039215686274 34.397347174163784 24.57179930795848 84-85 16.80795847750865 26.70487312572088 31.59818339100346 24.88898500576701 86-87 16.938597314128607 27.59476680834482 29.468815171348783 25.997820706177794 88-89 16.26476377952756 26.739810097267252 26.472759379342286 30.522666743862896 90-91 19.138974121461242 22.160307995136918 28.180541886180745 30.520175997221095 92-93 20.76268742791234 26.058246828143023 29.02681660899654 24.152249134948097 94-95 18.518598615916957 23.796136101499425 30.06559976931949 27.61966551326413 96-97 13.261245674740485 25.553633217993077 33.27782583621684 27.9072952710496 98-99 17.733819691748728 26.872503928834035 26.352744416730346 29.04093196268689 100-101 16.339506484331633 23.025685017913915 28.53682624586394 32.097982251890514 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1049.0 1 630.0 2 238.0 3 234.5 4 156.0 5 145.5 6 268.0 7 428.0 8 563.0 9 606.5 10 460.5 11 273.5 12 220.5 13 223.5 14 222.5 15 208.5 16 205.0 17 217.0 18 224.5 19 242.0 20 246.0 21 257.0 22 308.5 23 364.5 24 411.0 25 434.5 26 472.5 27 521.0 28 547.5 29 609.5 30 682.5 31 758.5 32 809.0 33 867.0 34 910.0 35 895.0 36 958.0 37 975.5 38 851.5 39 712.5 40 637.5 41 584.0 42 584.0 43 619.0 44 618.0 45 742.0 46 1639.5 47 5501.0 48 11931.5 49 11210.0 50 5961.0 51 4001.5 52 2640.0 53 1437.5 54 616.5 55 345.5 56 250.0 57 221.0 58 204.5 59 160.0 60 106.0 61 79.5 62 67.0 63 49.5 64 42.0 65 32.5 66 25.0 67 26.0 68 17.0 69 14.0 70 15.0 71 13.0 72 16.5 73 13.0 74 7.5 75 8.5 76 8.0 77 7.5 78 8.5 79 8.5 80 5.0 81 2.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3027681660899654 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.208765859284891E-4 16-17 0.002162629757785467 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.208765859284891E-4 26-27 0.0014417531718569781 28-29 0.0 30-31 0.002162629757785467 32-33 0.0 34-35 0.0057670126874279125 36-37 0.02739331026528258 38-39 0.017301038062283735 40-41 0.010092272202998848 42-43 0.009371395617070358 44-45 0.0028835063437139563 46-47 0.0014417531718569781 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.004325259515570934 60-61 0.00720876585928489 62-63 0.0028835063437139563 64-65 0.0014417531718569781 66-67 0.0028835063437139563 68-69 0.003604382929642445 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2104959630911188 78-79 0.0 80-81 0.004325259515570934 82-83 0.0 84-85 0.0 86-87 0.10308535178777394 88-89 0.39215686274509803 90-91 0.3863898500576701 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014417531718569781 100-101 7.208765859284891E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 69360.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.030565167243367 #Duplication Level Percentage of deduplicated Percentage of total 1 80.19236704042794 22.478373702422143 2 10.184137434420327 5.709342560553633 3 3.6107396358399346 3.036332179930796 4 1.8619483592223023 2.0876585928489044 5 0.9258306758563933 1.2975778546712802 6 0.7149470219113261 1.2024221453287198 7 0.4320543153996503 0.8477508650519031 8 0.21088365394506736 0.4728950403690888 9 0.19545314268079417 0.4930795847750865 >10 1.2395844048966156 6.634948096885814 >50 0.13887460137845903 2.8186274509803924 >100 0.18002263141652092 11.34083044982699 >500 0.046291533792819674 10.069204152249135 >1k 0.06686554881185064 31.510957324106116 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4903 7.068915801614764 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2800 4.036908881199539 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1846 2.6614763552479817 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1450 2.0905420991926182 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1372 1.978085351787774 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1333 1.9218569780853518 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1310 1.8886966551326414 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1219 1.7574971164936564 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1171 1.6882929642445212 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1158 1.6695501730103806 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1138 1.640715109573241 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1085 1.5643021914648212 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1071 1.5441176470588236 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 970 1.3985005767012688 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 967 1.3941753171856979 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 910 1.31199538638985 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 767 1.1058246828143021 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 735 1.0596885813148789 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 734 1.058246828143022 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 696 1.0034602076124566 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 689 0.9933679354094579 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 516 0.7439446366782008 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 482 0.6949250288350635 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 454 0.654555940023068 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 435 0.6271626297577855 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 421 0.6069780853517878 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 384 0.5536332179930795 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 371 0.5348904267589388 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 335 0.48298731257208766 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 299 0.43108419838523643 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 284 0.40945790080738176 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 257 0.37053056516724336 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 256 0.3690888119953864 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 254 0.3662053056516724 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.3344867358708189 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 227 0.327277970011534 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 210 0.3027681660899654 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 203 0.2926758938869665 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 193 0.2782583621683968 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 186 0.2681660899653979 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 185 0.2667243367935409 RNA PCR Primer, Index 27 (95% over 23bp) ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 182 0.26239907727797 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 165 0.2378892733564014 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164 0.2364475201845444 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 160 0.2306805074971165 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 159 0.2292387543252595 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 155 0.22347174163783162 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 147 0.21193771626297578 RNA PCR Primer, Index 27 (96% over 30bp) ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 143 0.20617070357554787 RNA PCR Primer, Index 27 (96% over 25bp) GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 132 0.1903114186851211 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 128 0.1845444059976932 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 119 0.1715686274509804 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 114 0.1643598615916955 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 112 0.16147635524798157 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 110 0.15859284890426759 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 107 0.15426758938869664 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 101 0.14561707035755478 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 99 0.14273356401384082 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 95 0.13696655132641292 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 95 0.13696655132641292 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 94 0.13552479815455595 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 91 0.131199538638985 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 91 0.131199538638985 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 89 0.12831603229527105 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 89 0.12831603229527105 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 86 0.12399077277970012 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 81 0.11678200692041521 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 80 0.11534025374855825 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 80 0.11534025374855825 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 75 0.10813148788927336 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.208765859284891E-4 0.0 20-21 0.0 0.0 0.0 0.004325259515570935 0.0 22-23 0.0 0.0 0.0 0.01513840830449827 0.0 24-25 0.0 0.0 0.0 0.022347174163783158 0.0 26-27 0.0 0.0 0.0 0.0526239907727797 0.0 28-29 0.0 0.0 0.0 0.11966551326412919 0.0 30-31 0.0 0.0 0.0 0.24653979238754326 0.0 32-33 0.0 0.0 0.0 0.5312860438292965 0.0 34-35 0.0 0.0 0.0 0.9515570934256056 0.0 36-37 0.0 0.0 0.0 1.484284890426759 0.0 38-39 0.0 0.0 0.0 2.110726643598616 0.0 40-41 0.0 0.0 0.0 2.7277970011534025 0.0 42-43 0.0 0.0 0.0 3.287918108419839 0.0 44-45 0.0 0.0 0.0 3.8819204152249136 0.0 46-47 0.0 0.0 0.0 4.571799307958478 0.0 48-49 0.0 0.0 0.0 5.175173010380623 0.0 50-51 0.0 0.0 0.0 5.77782583621684 0.0 52-53 0.0 0.0 0.0 6.242070357554787 0.0 54-55 0.0 0.0 0.0 6.811562860438293 0.0 56-57 0.0 0.0 0.0 7.658592848904267 0.0 58-59 0.0 0.0 0.0 8.217272202998846 0.0 60-61 0.0 0.0 0.0 8.872549019607844 0.0 62-63 0.0 0.0 0.0 9.449971164936564 0.0 64-65 0.0 0.0 0.0 10.013696655132641 0.0 66-67 0.0 0.0 0.0 10.714388696655133 0.0 68-69 0.0 0.0 0.0 11.567185697808537 0.0 70-71 0.0 0.0 0.0 12.416378316032294 0.0 72-73 0.0 0.0 0.0 13.07237600922722 0.0 74-75 0.0 0.0 0.0 13.907151095732411 0.0 76-77 0.0 0.0 0.0 14.604238754325259 0.0 78-79 0.0 0.0 0.0 15.97318339100346 0.0 80-81 0.0 0.0 0.0 17.07900807381776 0.0 82-83 0.0 0.0 0.0 17.9808246828143 0.0 84-85 0.0 0.0 0.0 18.985005767012687 0.0 86-87 0.0 0.0 0.0 20.028114186851212 0.0 88-89 0.0 0.0 0.0 21.128171856978085 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGTAC 20 1.743058E-5 94.881035 4 GCTTATG 25 5.1794137E-5 76.17945 1 TACACCA 20 0.0020761357 71.160774 5 GCTTACA 20 0.0020761357 71.160774 2 CTTATGT 30 1.300697E-4 63.25403 2 CACCACA 25 0.0050263205 56.928627 7 TTATGTA 35 2.7868623E-4 54.217735 3 GTAAGCG 25 2.8349696E-5 47.44052 94-95 GTTGATC 50 0.0016584005 23.72026 94-95 AGTAATG 50 0.0016584005 23.72026 12-13 AAGCAGG 70 1.7820887E-5 23.72026 94-95 TAATGGG 55 0.0028856702 21.563873 14-15 AGTACTT 770 0.0 19.407484 12-13 GAGTACT 845 0.0 19.088493 12-13 GTACTTT 850 0.0 17.580898 14-15 ACTTTTT 910 0.0 16.421717 16-17 TACTTTT 895 0.0 16.166882 14-15 GGGCTTG 135 1.6779435E-4 14.05645 20-21 CATGGGA 490 7.2486728E-9 13.554435 4 GCTTGAT 140 2.2843182E-4 13.554434 22-23 >>END_MODULE