##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939108_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68516 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.606121197968356 33.0 31.0 34.0 30.0 34.0 2 31.56398505458579 33.0 31.0 34.0 30.0 34.0 3 31.614309066495416 34.0 31.0 34.0 30.0 34.0 4 34.98530270301827 37.0 35.0 37.0 33.0 37.0 5 35.02037480296573 37.0 35.0 37.0 33.0 37.0 6 35.22562613112266 37.0 35.0 37.0 33.0 37.0 7 35.221685445735304 37.0 35.0 37.0 33.0 37.0 8 35.23738980676046 37.0 35.0 37.0 33.0 37.0 9 36.79213614338257 39.0 37.0 39.0 33.0 39.0 10-11 36.715694144433414 39.0 37.0 39.0 33.0 39.0 12-13 36.67206200011676 39.0 37.0 39.0 33.0 39.0 14-15 37.63130509661977 40.0 38.0 41.0 33.0 41.0 16-17 37.63949296514683 40.0 38.0 41.0 32.5 41.0 18-19 37.64995037655438 40.0 38.0 41.0 33.0 41.0 20-21 37.64129546383326 40.0 38.0 41.0 33.0 41.0 22-23 37.675929709848795 40.0 38.0 41.0 33.0 41.0 24-25 37.509333586315606 40.0 37.5 41.0 33.0 41.0 26-27 37.24051316480822 40.0 37.0 41.0 33.0 41.0 28-29 37.10142886333119 40.0 36.5 41.0 32.5 41.0 30-31 36.88272812189854 40.0 36.0 41.0 32.0 41.0 32-33 36.44263383735186 39.0 35.0 41.0 31.5 41.0 34-35 35.93654037013252 39.0 35.0 41.0 30.0 41.0 36-37 35.50245927958433 38.5 35.0 41.0 29.5 41.0 38-39 35.23706141631152 38.0 35.0 40.5 28.5 41.0 40-41 35.1072669157569 38.0 35.0 41.0 27.0 41.0 42-43 34.701682818611715 38.0 35.0 41.0 24.0 41.0 44-45 34.216883647615155 38.0 35.0 40.5 18.0 41.0 46-47 33.58435255998599 38.0 35.0 41.0 2.0 41.0 48-49 33.09074522739215 38.0 34.0 41.0 2.0 41.0 50-51 31.742374044018916 36.5 32.5 39.0 2.0 40.5 52-53 31.930337439430208 37.5 33.0 39.5 2.0 40.5 54-55 32.301630276139875 38.0 33.5 40.0 2.0 41.0 56-57 31.91803374394302 38.0 33.0 40.5 2.0 41.0 58-59 31.61765864907467 38.0 33.0 40.0 2.0 41.0 60-61 30.92601728063518 37.0 31.0 40.0 2.0 41.0 62-63 30.53832681417479 37.0 31.0 40.0 2.0 41.0 64-65 30.028263471305973 36.5 30.0 39.5 2.0 40.5 66-67 29.344481580944596 36.0 28.5 39.0 2.0 40.0 68-69 28.72569180921245 35.0 27.5 38.0 2.0 39.0 70-71 27.896279701091714 35.0 26.0 37.0 2.0 39.0 72-73 27.261522855975247 34.5 25.0 36.5 2.0 37.5 74-75 26.817947632669743 34.0 23.5 36.0 2.0 37.0 76-77 26.34094955922704 34.0 20.0 35.0 2.0 36.5 78-79 25.8237054118746 34.0 14.5 35.0 2.0 36.0 80-81 25.256918092124465 34.0 4.5 35.0 2.0 36.0 82-83 24.790764201062526 33.5 2.0 35.0 2.0 35.5 84-85 24.349962052659233 33.0 2.0 35.0 2.0 35.0 86-87 24.101063985054587 33.0 2.0 35.0 2.0 35.0 88-89 23.564751298966662 33.0 2.0 35.0 2.0 35.0 90-91 23.01921449004612 32.0 2.0 35.0 2.0 35.0 92-93 22.560117344853758 32.0 2.0 35.0 2.0 35.0 94-95 21.945034736411934 31.0 2.0 35.0 2.0 35.0 96-97 21.274651176367563 31.0 2.0 34.5 2.0 35.0 98-99 20.77473874715395 31.0 2.0 34.0 2.0 35.0 100-101 18.411735944888786 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04885825893358842 1101 2 0.24663998569113232 1101 3 0.28336515471433543 1101 4 0.22781220280821657 1101 5 -0.5539599205092571 1101 6 -0.15547361788561886 1101 7 0.10323903017651048 1101 8 0.08552938894969486 1101 9 0.2491795681316873 1101 10-11 0.3022636897052422 1101 12-13 -0.028713245217552696 1101 14-15 0.3614331459532494 1101 16-17 0.49874342498020496 1101 18-19 0.42994013646101337 1101 20-21 0.49339970265396715 1101 22-23 0.10408602866430527 1101 24-25 -0.018779277571191244 1101 26-27 -0.5184235582323424 1101 28-29 -0.9050100190831003 1101 30-31 -0.25247612869346625 1101 32-33 0.06718806969542612 1101 34-35 0.007283449918801921 1101 36-37 -0.670732304762879 1101 38-39 -0.9374769200901696 1101 40-41 -1.0433048951236046 1101 42-43 -1.953348882511932 1101 44-45 -2.8106206948603933 1101 46-47 -2.5420615023426407 1101 48-49 -3.1605283699083273 1101 50-51 -3.029668663271025 1101 52-53 -2.962718700579245 1101 54-55 -2.4969906429823894 1101 56-57 -2.4238013542661108 1101 58-59 -2.4797170345840946 1101 60-61 -2.91661041570654 1101 62-63 -2.3357950516926813 1101 64-65 -2.3418289039985147 1101 66-67 -2.456349081827252 1101 68-69 -2.2223251604457133 1101 70-71 -2.5046994046393927 1101 72-73 -1.5702861902957501 1101 74-75 -1.8994301058767462 1101 76-77 -1.9462661028658097 1101 78-79 -1.3889006170829994 1101 80-81 -0.9014024774476326 1101 82-83 -0.5188737666276033 1101 84-85 -0.37457035352476353 1101 86-87 -0.4987638829692145 1101 88-89 -0.39114667199343245 1101 90-91 -1.1073381871597938 1101 92-93 -0.9370665743353079 1101 94-95 -1.229735494375987 1101 96-97 -2.1911577335973256 1101 98-99 -2.136212465167933 1101 100-101 -0.31686305088562605 1102 1 1.087724419073858 1102 2 0.7738170751125217 1102 3 0.9619157099451918 1102 4 0.4607169368818589 1102 5 0.8946081683276788 1102 6 0.5189857625936369 1102 7 0.6894459267282329 1102 8 0.7734312007556525 1102 9 0.7716810292655296 1102 10-11 0.8005103290974134 1102 12-13 0.43329727494609216 1102 14-15 0.5713980787410904 1102 16-17 1.2396843951730787 1102 18-19 1.1548086344154314 1102 20-21 1.3274148984459018 1102 22-23 1.2405559293071988 1102 24-25 1.0203791093370498 1102 26-27 1.2296769677758448 1102 28-29 0.9871583035352529 1102 30-31 1.3606156305116741 1102 32-33 1.5299291567789979 1102 34-35 1.742672111520207 1102 36-37 2.050483358591876 1102 38-39 1.843353003930872 1102 40-41 2.0883432638477615 1102 42-43 2.650829960270073 1102 44-45 3.0365447054902788 1102 46-47 3.4294463877800965 1102 48-49 3.9908450959011432 1102 50-51 3.9478298755951364 1102 52-53 3.8070066062588594 1102 54-55 3.841407954329128 1102 56-57 4.445281053682457 1102 58-59 4.309587465708137 1102 60-61 3.8154176380631846 1102 62-63 3.1389273328777456 1102 64-65 3.0230162158144616 1102 66-67 3.9723475867875955 1102 68-69 3.9253954707641014 1102 70-71 3.6276793212519003 1102 72-73 4.338100717945043 1102 74-75 4.462931086408467 1102 76-77 3.7296544114533035 1102 78-79 4.023285239141433 1102 80-81 3.77568694394337 1102 82-83 3.8469941468732713 1102 84-85 4.217334965002415 1102 86-87 4.845333136317752 1102 88-89 4.6887773490468945 1102 90-91 4.782638434698772 1102 92-93 4.025820626132255 1102 94-95 3.926898053256977 1102 96-97 3.4670830612594834 1102 98-99 3.284301853943699 1102 100-101 2.640764067758443 1103 1 0.5792498428026676 1103 2 0.6043255496887916 1103 3 0.21615299808078703 1103 4 0.12173388603439861 1103 5 0.3691844395141217 1103 6 0.23085016937329783 1103 7 0.7911408419824681 1103 8 0.5022447600776871 1103 9 0.924223402146886 1103 10-11 0.9361035494363961 1103 12-13 1.2638057495223691 1103 14-15 1.4019065533173674 1103 16-17 1.2396843951730787 1103 18-19 1.2226052445849263 1103 20-21 1.2172454069204761 1103 22-23 0.9778440649004239 1103 24-25 0.9610570754387453 1103 26-27 1.5517108660809242 1103 28-29 1.4532599984505126 1103 30-31 1.4029885118676049 1103 32-33 1.7587427161010325 1103 34-35 2.1070788911812244 1103 36-37 2.2199748840156133 1103 38-39 2.1569123259647753 1103 40-41 1.6052924163901352 1103 42-43 1.4982875873887167 1103 44-45 1.6890870783716352 1103 46-47 2.3616497776106016 1103 48-49 1.8128789942062227 1103 50-51 2.0410502145781813 1103 52-53 1.5951421994792057 1103 54-55 1.6380181238206433 1103 56-57 0.9791793587671975 1103 58-59 0.8095874657081374 1103 60-61 0.9001634007750461 1103 62-63 0.1389273328777456 1103 64-65 -0.036305818083846475 1103 66-67 -0.03612698948359139 1103 68-69 0.8237005555098662 1103 70-71 0.8988657619298657 1103 72-73 0.3296261416738595 1103 74-75 -1.7150350152864462 1103 76-77 -1.2703455885467 1103 78-79 -1.4767147608585667 1103 80-81 -1.3938045814803601 1103 82-83 -1.5513109378724899 1103 84-85 -1.8419870688958895 1103 86-87 -2.3411075416483484 1103 88-89 -1.2942734984107318 1103 90-91 -1.242785294114789 1103 92-93 -0.9063827636982538 1103 94-95 -0.5053053365735352 1103 96-97 -0.482069481113399 1103 98-99 -0.2241727223274914 1103 100-101 0.7255098304703047 1104 1 0.696800416886866 1104 2 0.9468624551015843 1104 3 0.9307511391414742 1104 4 0.5014495776691703 1104 5 0.6212347402248923 1104 6 0.7182749916805662 1104 7 0.8184781847927525 1104 8 0.38141370485625714 1104 9 0.705798033092762 1104 10-11 0.6342992848109219 1104 12-13 0.7782945412118139 1104 14-15 0.9671061159198828 1104 16-17 1.052970343778874 1104 18-19 0.7455686125455543 1104 20-21 1.0067478672813408 1104 22-23 0.567100489175985 1104 24-25 0.751242969369855 1104 26-27 1.0388623149600988 1104 28-29 1.184533918625469 1104 30-31 1.532840890216427 1104 32-33 1.3124605947232268 1104 34-35 1.5968273876273713 1104 36-37 1.614862800910089 1104 38-39 1.5266498874737877 1104 40-41 1.4013558390254488 1104 42-43 1.5802996158195555 1104 44-45 1.059234700022813 1104 46-47 1.1720653052213237 1104 48-49 1.1092157902696513 1104 50-51 1.3524225491872635 1104 52-53 1.6849453706109685 1104 54-55 1.6862685338807921 1104 56-57 0.9165768437316615 1104 58-59 0.2747323536250299 1104 60-61 -0.8479229852282266 1104 62-63 -0.3191098459084749 1104 64-65 -0.3401603834200948 1104 66-67 -0.2842133754868712 1104 68-69 -0.25680791906640366 1104 70-71 0.31370993907584577 1104 72-73 0.2503478475240506 1104 74-75 -1.0927004062104473 1104 76-77 -1.0588912419091905 1104 78-79 -0.6344512288738748 1104 80-81 -0.5579653250560845 1104 82-83 0.3979782912144394 1104 84-85 -0.016672689453571365 1104 86-87 0.18199251302633712 1104 88-89 0.14695121454716897 1104 90-91 1.3609599218502204 1104 92-93 0.8571492209928344 1104 94-95 -0.3109368291924497 1104 96-97 0.26601690488988083 1104 98-99 1.3676807495150491 1104 100-101 -0.9539598250791741 1105 1 0.9813059695045325 1105 2 0.6615636713892634 1105 3 0.3834216838268283 1105 4 0.8380439721694373 1105 5 0.6328687962798867 1105 6 0.34254786317713126 1105 7 0.8533803529577426 1105 8 0.9996051379604367 1105 9 0.9947985619134911 1105 10-11 0.9365203318759683 1105 12-13 0.5085959690277875 1105 14-15 0.8149379509278205 1105 16-17 0.2962278794742659 1105 18-19 0.6807880731484133 1105 20-21 1.0445585494600778 1105 22-23 0.4918757403658347 1105 24-25 0.6604180090314102 1105 26-27 0.7342615746110752 1105 28-29 0.46242921212097343 1105 30-31 1.2723966808034177 1105 32-33 0.9262892568067898 1105 34-35 1.1247226811228757 1105 36-37 1.6105000298894652 1105 38-39 0.6150951545282553 1105 40-41 0.42065779566215866 1105 42-43 0.20139956293748185 1105 44-45 -0.05737027089205071 1105 46-47 -0.24768614903568675 1105 48-49 -0.13113323141200084 1105 50-51 -0.5457794603315094 1105 52-53 -0.9095813348358845 1105 54-55 -0.564260286848981 1105 56-57 -0.38564420333338134 1105 58-59 0.16301897446056657 1105 60-61 0.3959955763793843 1105 62-63 0.19741580189691987 1105 64-65 0.8069839846391318 1105 66-67 1.369541251694514 1105 68-69 0.9931920809335963 1105 70-71 1.510563455733699 1105 72-73 1.78683647787836 1105 74-75 1.6929949930492008 1105 76-77 1.0887819468798092 1105 78-79 0.9628517854042826 1105 80-81 1.5468733846213354 1105 82-83 0.9279888676290362 1105 84-85 1.3757122920377007 1105 86-87 1.31935369758477 1105 88-89 0.7915836841399724 1105 90-91 0.28652840413472447 1105 92-93 -0.02016436969991986 1105 94-95 0.339512668427858 1105 96-97 0.7024262121347249 1105 98-99 1.1905258050412222 1105 100-101 1.3880549819012593 1106 1 0.17008670720944963 1106 2 -0.47803673844680006 1106 3 0.14597291333502582 1106 4 0.047316513153042195 1106 5 0.16155732087005248 1106 6 -0.0498701696097541 1106 7 0.033195926728232905 1106 8 -0.047013714498582715 1106 9 0.061140775028242444 1106 10-11 0.003502913843178135 1106 12-13 -0.3247800555623783 1106 14-15 0.35873918043601094 1106 16-17 0.5267606663595146 1106 18-19 0.040931515771362115 1106 20-21 0.2940462543781095 1106 22-23 -0.2504398334046627 1106 24-25 0.27594055001501516 1106 26-27 -0.02893002374958087 1106 28-29 0.12354601539966126 1106 30-31 0.2011876644099786 1106 32-33 0.048599707626458155 1106 34-35 0.3795894844015635 1106 36-37 1.209116833168153 1106 38-39 0.6705510971512112 1106 40-41 1.2864364841867442 1106 42-43 1.6697653416260039 1106 44-45 0.7346379258292615 1106 46-47 0.7612790148987401 1106 48-49 0.8408186128502919 1106 50-51 0.6583701298324236 1106 52-53 1.3338869028690326 1106 54-55 1.1091515983969202 1106 56-57 1.0004982146994053 1106 58-59 1.0267484826572861 1106 60-61 1.2730447567072538 1106 62-63 0.8915756379624931 1106 64-65 1.0980956649670013 1106 66-67 1.2898793664486128 1106 68-69 1.4931920809335963 1106 70-71 1.7275204229468137 1106 72-73 1.9620413959111467 1106 74-75 0.948630238950841 1106 76-77 1.4564817419617775 1106 78-79 1.2660024001583814 1106 80-81 0.9921858846213354 1106 82-83 1.004833129924119 1106 84-85 1.0712809395786849 1106 86-87 0.4670227549618211 1106 88-89 0.6290580693858772 1106 90-91 0.46378250249538056 1106 92-93 -0.2985967467491015 1106 94-95 0.6288313159688386 1106 96-97 -0.011200837045603151 1106 98-99 0.5881948624182733 1106 100-101 0.940949449114374 1107 1 0.2965071617549029 1107 2 0.3197852312501688 1107 3 0.0905751860622992 1107 4 0.3782824222439487 1107 5 0.4721633814761148 1107 6 0.44066013342055044 1107 7 1.3106580479403576 1107 8 1.1717362855014173 1107 9 1.2131294113918756 1107 10-11 1.0018457168734827 1107 12-13 0.6920286565588398 1107 14-15 0.8251217561935889 1107 16-17 0.949109151207999 1107 18-19 1.0002118188016595 1107 20-21 1.0563568604387186 1107 22-23 1.2315677423529152 1107 24-25 0.9582322166816866 1107 26-27 0.5285983853413256 1107 28-29 -0.4564729239942764 1107 30-31 -0.1709714264991149 1107 32-33 0.01285160156584908 1107 34-35 -0.17296733378024953 1107 36-37 -0.14173543955912038 1107 38-39 -0.7245720089093979 1107 40-41 -0.8745483642981 1107 42-43 -0.4204335220103559 1107 44-45 -0.4263469226555827 1107 46-47 -0.45794446994974436 1107 48-49 -0.5476757053315211 1107 50-51 -0.584527597440303 1107 52-53 -0.8292286274339986 1107 54-55 -1.2663218864515642 1107 56-57 -0.8146059519672626 1107 58-59 -0.6759030324942294 1107 60-61 0.01546899913149602 1107 62-63 0.17282563796249306 1107 64-65 -0.1720274410936078 1107 66-67 0.4579664876607339 1107 68-69 0.2886466263881431 1107 70-71 0.5783726956740871 1107 72-73 0.23666260803235772 1107 74-75 0.7904862995569033 1107 76-77 0.9936503025678398 1107 78-79 0.9679436880371703 1107 80-81 1.09990368765164 1107 82-83 0.8855149481059392 1107 84-85 0.6766313183665638 1107 86-87 0.015081467083032152 1107 88-89 1.006188751204057 1107 90-91 1.3179491691620449 1107 92-93 1.070247950220594 1107 94-95 0.7843710886961119 1107 96-97 0.675352193257428 1107 98-99 0.5832232715091834 1107 100-101 -0.47595395997653256 1108 1 0.7026174449143667 1108 2 0.2681210484384451 1108 3 0.25227414284322336 1108 4 0.39542102134976176 1108 5 0.5345081405421865 1108 6 0.22779376481647517 1108 7 0.3287901890233158 1108 8 0.1143592363210928 1108 9 0.5521756110938156 1108 10-11 0.41193016794153436 1108 12-13 0.7053172805031949 1108 14-15 0.011659262403220794 1108 16-17 0.19786722373655863 1108 18-19 0.5250503682303815 1108 20-21 0.6920995330666315 1108 22-23 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2316 88-89 -1.9256294306141228 2316 90-91 -2.2128479322872288 2316 92-93 -2.115852181531711 2316 94-95 -1.8850953144659428 2316 96-97 -1.6701682283499508 2316 98-99 -1.7117372027991173 2316 100-101 -1.1592543552334504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1129.0 3 190.0 4 153.0 5 107.0 6 139.0 7 146.0 8 159.0 9 196.0 10 206.0 11 300.0 12 465.0 13 721.0 14 726.0 15 808.0 16 1134.0 17 1634.0 18 1232.0 19 936.0 20 976.0 21 1039.0 22 971.0 23 889.0 24 920.0 25 925.0 26 957.0 27 1056.0 28 1134.0 29 1323.0 30 1509.0 31 1820.0 32 2181.0 33 2620.0 34 3614.0 35 6326.0 36 8306.0 37 12098.0 38 8920.0 39 551.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.91625399244 18.75787499633721 14.183784100565536 13.14208691065725 2 14.653963058218377 28.013210194061255 31.816986205284074 25.515840542436287 3 31.51934064970554 26.375473104294837 30.7448378658795 11.36034838012012 4 12.54329431364812 18.492700249901358 39.395267949785904 29.56873748666462 5 9.051982404606369 43.19639908223362 32.718079120814885 15.033539392345126 6 23.337130838664446 37.12937808547308 14.617474366839014 24.916016709023456 7 19.62313759859772 39.173239848086475 22.75927548933684 18.444347063978967 8 23.399500460102537 23.433095248528403 19.37396842089888 33.79343587047018 9 19.670766264497093 25.57769273465572 27.235546726651283 27.515994274195904 10-11 25.44183804627249 24.98904533769572 22.920805094648284 26.6483115213835 12-13 34.09993865209898 25.020449300341795 23.752592679150478 17.127019368408753 14-15 25.502081355437085 27.672533411232013 32.55312933615716 14.27225589717374 16-17 16.101775372638375 30.892652396497454 32.42045147485193 20.58512075601224 18-19 15.439055207823603 34.874633907638824 31.636222876299126 18.050088008238447 20-21 20.96010867501205 26.561838126816728 28.455617066651083 24.022436131520138 22-23 17.018230688325346 27.52424915274045 32.296657707140355 23.160862451793854 24-25 24.219138056975893 27.876552227903577 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18.92238849765258 50-51 12.953345070422534 31.487676056338028 37.091402582159624 18.467576291079812 52-53 17.812972227520135 34.12435263567142 27.495928756913777 20.56674637989466 54-55 14.746246323340644 28.058503810522765 31.10178753493285 26.093462331203742 56-57 20.865598967499707 26.01636747624076 29.275196527044468 23.842837029215065 58-59 25.725912890453145 25.64012318521777 28.744683971256784 19.889279953072297 60-61 19.24663808581584 28.358799604265144 34.560111392034 17.834450917885018 62-63 16.006952848247487 28.54481580086104 33.73451561090453 21.713715739986945 64-65 15.534265467509794 34.326354553323846 29.53021611232559 20.609163866840767 66-67 19.159348197712383 27.361169194198048 26.903352188937557 26.576130419152012 68-69 16.29405161091504 27.636859907989642 30.736889991121807 25.33219848997351 70-71 23.116827847321076 27.532541860499244 27.601514462233833 21.74911582994585 72-73 20.652516819145582 22.768391011202983 35.80625518147069 20.772836988180746 74-75 16.397038863087772 32.58277756990147 29.766909515110164 21.2532740519006 76-77 18.680955943840498 25.72730073500286 29.50721065680795 26.084532664348693 78-79 19.81520184798152 26.520734792652075 31.995746709199572 21.668316650166833 80-81 22.697037922512227 24.81280755663442 30.401079519203268 22.08907500165008 82-83 19.61241674735205 24.111727254056277 34.42918757151659 21.84666842707508 84-85 19.050343249427918 27.457753916563988 31.91632928475034 21.575573549257758 86-87 19.37334057003711 28.378636076924206 29.767789822651864 22.48023353038682 88-89 19.216465807483257 27.992166125092606 26.486272381189895 26.305095686234235 90-91 22.227846986838053 23.292394829129435 28.132382503558272 26.34737568047424 92-93 24.876924995781334 27.13886382145137 28.422805743255637 19.561405439511663 94-95 22.716368198258344 25.364067876191243 29.301503224339175 22.61806070121124 96-97 18.233641225798404 26.35598629523928 32.409411384929165 23.000961094033144 98-99 22.662910302123226 28.323869055479744 25.624715704831917 23.388504937565113 100-101 21.90754921808526 23.924683859988853 27.408092010679812 26.759674911246073 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1167.0 1 688.5 2 247.5 3 246.0 4 174.0 5 169.5 6 259.5 7 411.0 8 536.5 9 566.5 10 413.5 11 255.0 12 237.0 13 222.5 14 219.5 15 248.5 16 293.0 17 289.0 18 282.5 19 287.0 20 302.0 21 326.5 22 353.5 23 421.0 24 480.5 25 513.5 26 541.0 27 629.0 28 694.5 29 744.5 30 834.5 31 854.5 32 813.5 33 818.0 34 838.5 35 786.0 36 741.0 37 724.0 38 635.5 39 508.5 40 449.0 41 426.0 42 414.5 43 421.5 44 437.5 45 603.5 46 1821.0 47 5494.0 48 12172.0 49 12216.0 50 5990.0 51 2829.0 52 1501.0 53 930.5 54 640.0 55 500.0 56 395.0 57 309.0 58 244.5 59 211.0 60 186.0 61 138.5 62 118.5 63 115.0 64 97.5 65 88.5 66 84.5 67 73.5 68 57.5 69 52.5 70 48.0 71 42.0 72 36.5 73 31.5 74 27.0 75 22.0 76 19.5 77 17.0 78 15.0 79 14.5 80 13.0 81 6.5 82 5.0 83 4.5 84 3.0 85 1.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38239243388405625 2 0.12259910093992644 3 0.12405861404635413 4 0.1298966664720649 5 0.1298966664720649 6 0.0729756553213848 7 0.08173273395995095 8 0.07735419464066787 9 0.07881370774709558 10-11 0.07589468153424019 12-13 0.07881370774709558 14-15 0.07443516842781248 16-17 0.07516492498102632 18-19 0.0824624905131648 20-21 0.08027322085352326 22-23 0.08757078638566174 24-25 0.09632786502422792 26-27 0.0977873781306556 28-29 0.09267908225815867 30-31 0.08392200361959251 32-33 0.08465176017280635 34-35 0.32839044894623154 36-37 0.5268842314203982 38-39 0.5064510479304105 40-41 0.47653102924864266 42-43 0.5071808044836243 44-45 0.5195866658882596 46-47 0.5173973962286181 48-49 0.5195866658882596 50-51 0.5195866658882596 52-53 0.518127152781832 54-55 0.5100998306964797 56-57 0.484558351333995 58-59 0.4758012726954288 60-61 0.4210695312043902 62-63 0.4998832389514858 64-65 0.5210461789946874 66-67 0.5349115535057505 68-69 0.5422091190378889 70-71 0.5443983886975305 72-73 0.531992527292895 74-75 0.5349115535057505 76-77 0.5152081265689765 78-79 0.4867476209936365 80-81 0.4925856734193473 82-83 0.5108295872496935 84-85 0.5020725086111274 86-87 0.5035320217175551 88-89 0.5115593438029074 90-91 0.5327222838461089 92-93 0.5349115535057505 94-95 0.5290735010800397 96-97 0.531992527292895 98-99 0.5327222838461089 100-101 0.5108295872496935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 68516.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.786969466985813 #Duplication Level Percentage of deduplicated Percentage of total 1 81.93325116146771 25.224765018389867 2 9.187446667298758 5.657072800513749 3 3.6029202616857874 3.3276898826551466 4 1.6592395941974023 2.043318348998774 5 0.9339148573053948 1.4376204098312801 6 0.4788091400398218 0.8844649424951836 7 0.41718024082677535 0.8990600735594606 8 0.25125628140703515 0.6188335571253429 9 0.15644259031004076 0.43347539260902557 >10 1.01924717929269 6.151847743592738 >50 0.12799848298094246 2.9292428046003853 >100 0.118517113871243 7.801097553856033 >500 0.05214753010334692 11.438204215073851 >1k 0.06162889921304636 31.153307256699165 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4551 6.642244147352443 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2890 4.217992877576041 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1938 2.8285364002568745 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1472 2.148403292661568 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1370 1.9995329558059431 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1315 1.91925973495242 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1231 1.7966606340124935 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1146 1.6726020199661393 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1139 1.6623854282211452 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1110 1.6200595481347422 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1081 1.5777336680483391 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1079 1.5748146418354838 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1023 1.4930819078755326 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 987 1.4405394360441357 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 904 1.3193998482106368 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 848 1.2376671142506859 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 778 1.1355011968007473 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 750 1.0946348298207718 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 689 1.0056045303286825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 652 0.9516025453908576 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 638 0.93116936190087 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 552 0.8056512347480881 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 534 0.7793799988323895 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 505 0.7370541187459864 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 476 0.6947282386595832 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 444 0.648023819253897 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 374 0.5458579018039582 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 290 0.4232588008640317 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 265 0.38677097320333936 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 256 0.37363535524549013 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 242 0.3532021717555024 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 230 0.33568801447837004 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 221 0.32255239652052076 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 218 0.31817385720123764 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 215 0.3137953178819546 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 205 0.2992001868176776 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 195 0.28460505575340067 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 190 0.27730749022126217 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 174 0.2539552805184191 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 172 0.2510362543055637 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 166 0.2422791756669975 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 163 0.2379006363477144 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 142 0.20725086111273278 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 0.2043318348998774 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 121 0.1766010858777512 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 117 0.17076303345204039 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 113 0.1649249810263296 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 110 0.16054644170704652 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 106 0.15470838928133573 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 98 0.14303228442991417 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 0.1415727713234865 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 92 0.134275205791348 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 92 0.134275205791348 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 91 0.1328156926849203 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 87 0.12697764025920955 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 87 0.12697764025920955 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 86 0.12551812715278185 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 83 0.12113958783349875 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 83 0.12113958783349875 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 81 0.11822056162064334 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 78 0.11384202230136026 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 77 0.11238250919493258 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 75 0.10946348298207717 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 72 0.1050849436627941 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 71 0.10362543055636639 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 69 0.10070640434351101 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0036487827660692394 0.0 22-23 0.0 0.0 0.0 0.008027322085352326 0.0 24-25 0.0 0.0 0.0 0.018973670383560043 0.0 26-27 0.0 0.0 0.0 0.04670441940568626 0.0 28-29 0.0 0.0 0.0 0.10946348298207717 0.0 30-31 0.0 0.0 0.0 0.26490162881662677 0.0 32-33 0.0 0.0 0.0 0.5290735010800397 0.0 34-35 0.0 0.0 0.0 0.9027088563255299 0.0 36-37 0.0 0.0 0.0 1.5040282561737404 0.0 38-39 0.0 0.0 0.0 2.149862805767996 0.0 40-41 0.0 0.0 0.0 2.7314787786794326 0.0 42-43 0.0 0.0 0.0 3.270039114951252 0.0 44-45 0.0 0.0 0.0 3.860412166501255 0.0 46-47 0.0 0.0 0.0 4.559518944480121 0.0 48-49 0.0 0.0 0.0 5.182731040924748 0.0 50-51 0.0 0.0 0.0 5.791348006305096 0.0 52-53 0.0 0.0 0.0 6.286852705937299 0.0 54-55 0.0 0.0 0.0 6.902767236849787 0.0 56-57 0.0 0.0 0.0 7.736878977173215 0.0 58-59 0.0 0.0 0.0 8.344036429447137 0.0 60-61 0.0 0.0 0.0 8.94389631618892 0.0 62-63 0.0 0.0 0.0 9.512376671142507 0.0 64-65 0.0 0.0 0.0 10.122453149629283 0.0 66-67 0.0 0.0 0.0 10.847831163523848 0.0 68-69 0.0 0.0 0.0 11.703835600443693 0.0 70-71 0.0 0.0 0.0 12.55473174149104 0.0 72-73 0.0 0.0 0.0 13.223918500788137 0.0 74-75 0.0 0.0 0.0 14.100356121197969 0.0 76-77 0.0 0.0 0.0 14.806760464708972 0.0 78-79 0.0 0.0 0.0 16.024724152022884 0.0 80-81 0.0 0.0 0.0 17.2164166034211 0.0 82-83 0.0 0.0 0.0 18.060744935489524 0.0 84-85 0.0 0.0 0.0 19.06707922237142 0.0 86-87 0.0 0.0 0.0 20.0464125167844 0.0 88-89 0.0 0.0 0.0 21.169507852180512 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCAT 40 7.562503E-6 59.25192 2 CAGACCC 25 0.005042376 56.881844 4 GTACCCA 45 1.493224E-5 52.823055 1 ACCCATG 45 1.519418E-5 52.668377 3 GGGCGCC 35 0.008301342 27.186102 90-91 GAGTACC 65 1.02718095E-5 25.467972 12-13 GTACTTT 670 0.0 24.001799 14-15 TAGTGTG 50 0.001631008 23.78784 94-95 AGTACTC 120 1.7376037E-4 23.64883 7 AGTACTT 615 0.0 22.687496 12-13 ACTTTTT 750 0.0 22.387562 16-17 CATGGGG 170 3.1336695E-6 22.306604 4 GAGTACT 725 0.0 22.180973 12-13 GTACATG 800 0.0 20.799076 1 TACATGG 780 0.0 20.662209 2 TACTTTT 685 0.0 20.369066 14-15 GCTTGAT 165 6.170872E-5 20.168802 1 ACATGGG 800 0.0 20.145653 3 TGGGCTT 145 6.2423793E-4 19.571445 6 ATGGGCT 150 7.727021E-4 18.960615 5 >>END_MODULE