##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939108_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68516 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.141733317765194 33.0 31.0 34.0 30.0 34.0 2 32.31499211862923 34.0 31.0 34.0 30.0 34.0 3 32.385384435752236 34.0 31.0 34.0 30.0 34.0 4 35.873591569852294 37.0 35.0 37.0 35.0 37.0 5 35.85947807811314 37.0 35.0 37.0 35.0 37.0 6 36.017966606340124 37.0 35.0 37.0 35.0 37.0 7 35.979099772315955 37.0 35.0 37.0 35.0 37.0 8 36.03718839395178 37.0 35.0 37.0 35.0 37.0 9 37.61134625488937 39.0 37.0 39.0 35.0 39.0 10-11 37.512778037246775 39.0 37.0 39.0 34.5 39.0 12-13 37.49235215132232 39.0 37.0 39.0 35.0 39.0 14-15 38.49383355712534 40.0 38.0 41.0 34.0 41.0 16-17 38.71249781073034 40.0 38.0 41.0 34.5 41.0 18-19 38.82792340475217 40.0 38.0 41.0 35.0 41.0 20-21 38.82955805943137 40.0 38.0 41.0 35.0 41.0 22-23 38.76050849436628 40.0 38.0 41.0 35.0 41.0 24-25 38.559124875941386 40.0 38.0 41.0 34.5 41.0 26-27 38.20574756261311 40.0 37.0 41.0 34.0 41.0 28-29 38.024476034794795 40.0 37.0 41.0 34.0 41.0 30-31 37.80182001284372 40.0 36.5 41.0 33.0 41.0 32-33 37.343160721583274 39.0 35.0 41.0 33.0 41.0 34-35 36.98791523147878 39.0 35.0 41.0 33.0 41.0 36-37 36.65614600969117 39.0 35.0 41.0 32.0 41.0 38-39 36.37626247883706 39.0 35.0 41.0 31.0 41.0 40-41 36.26435431140172 39.0 35.0 41.0 31.0 41.0 42-43 35.825726837527 39.0 35.0 41.0 30.0 41.0 44-45 35.347021133749784 39.0 35.0 41.0 28.0 41.0 46-47 34.70373343452624 38.0 35.0 40.0 23.5 41.0 48-49 33.99896374569444 38.0 34.0 40.0 18.5 41.0 50-51 33.510690933504584 38.0 34.0 40.0 10.0 41.0 52-53 33.263675637807225 38.0 34.0 40.0 2.0 41.0 54-55 33.007115126393835 38.0 33.5 40.0 2.0 41.0 56-57 32.484492673244205 37.5 33.0 40.0 2.0 41.0 58-59 31.947545098954986 37.5 32.5 40.0 2.0 41.0 60-61 31.159896958374688 36.0 31.0 39.5 2.0 41.0 62-63 30.70183606748789 36.0 31.0 39.0 2.0 41.0 64-65 30.105092241228327 35.5 29.5 39.0 2.0 40.5 66-67 29.35978457586549 35.0 28.5 38.5 2.0 39.5 68-69 28.62643762040983 35.0 27.5 37.0 2.0 39.0 70-71 27.79033364469613 34.0 26.0 36.5 2.0 38.0 72-73 27.08131677272462 34.0 25.0 36.0 2.0 37.0 74-75 26.631122657481463 34.0 24.5 35.0 2.0 37.0 76-77 24.86420690057797 31.0 20.5 34.0 2.0 35.0 78-79 25.72548747737755 32.5 21.0 35.0 2.0 36.0 80-81 25.640375970576216 33.0 20.0 35.0 2.0 35.5 82-83 25.2705499445385 33.0 19.0 35.0 2.0 35.0 84-85 24.86122219627532 32.0 14.5 35.0 2.0 35.0 86-87 24.45253663377897 32.0 4.5 35.0 2.0 35.0 88-89 23.776358806702085 31.5 2.0 34.0 2.0 35.0 90-91 23.258202463658122 31.0 2.0 34.0 2.0 35.0 92-93 22.86213439196684 31.0 2.0 34.0 2.0 35.0 94-95 22.359551053768463 31.0 2.0 34.0 2.0 35.0 96-97 21.57245023060307 30.0 2.0 34.0 2.0 35.0 98-99 20.60366921594956 29.0 2.0 34.0 2.0 35.0 100-101 15.663487360616498 13.5 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3467833441446828 1101 2 0.6772121233028514 1101 3 0.5492127524656283 1101 4 0.4504923753030283 1101 5 0.37964742838838106 1101 6 0.6564558933237095 1101 7 0.24186796608994143 1101 8 0.4428437906061973 1101 9 0.5396762885166595 1101 10-11 0.7011092609365264 1101 12-13 0.35621904064517906 1101 14-15 0.7730727073663246 1101 16-17 0.9272981184395803 1101 18-19 1.0211543440029516 1101 20-21 0.8913057097603954 1101 22-23 0.9550282332000606 1101 24-25 1.1073431571366683 1101 26-27 1.200585259695103 1101 28-29 1.1520582514757223 1101 30-31 0.9168870165587606 1101 32-33 1.0115575657982276 1101 34-35 0.7765037616788106 1101 36-37 0.9991089799278754 1101 38-39 0.3320523416445482 1101 40-41 -0.4174302291366274 1101 42-43 0.017895935209395475 1101 44-45 0.38561377923746676 1101 46-47 0.9337431199912487 1101 48-49 1.6365942717450395 1101 50-51 1.4067634328025704 1101 52-53 0.21273480660732247 1101 54-55 -0.10941726812554009 1101 56-57 -0.13902656731693952 1101 58-59 -0.35132205436577024 1101 60-61 -0.6505174851635154 1101 62-63 -0.2532526363654526 1101 64-65 -0.16676911217761514 1101 66-67 -0.38604334812532315 1101 68-69 0.3025289495130927 1101 70-71 -0.28284172908507443 1101 72-73 0.008099651384693374 1101 74-75 0.18870745001579792 1101 76-77 0.09378737249814506 1101 78-79 -0.29899957634549423 1101 80-81 -0.007242156959406998 1101 82-83 -0.25256680901443573 1101 84-85 0.10762180318646486 1101 86-87 0.06091912506389008 1101 88-89 -0.37585660224477024 1101 90-91 0.13978575210300193 1101 92-93 1.1635910007800874 1101 94-95 1.6088055675522313 1101 96-97 -0.016840286513218672 1101 98-99 0.11922204081212584 1101 100-101 0.2882924032052081 1102 1 0.5753046766987495 1102 2 0.23624193043318797 1102 3 0.24500468700682632 1102 4 0.15738892702716356 1102 5 0.3247087960096593 1102 6 0.3396820768421165 1102 7 0.37366223844529145 1102 8 0.24880521667282096 1102 9 0.6042584042384576 1102 10-11 0.4338678816261776 1102 12-13 0.4941500751279406 1102 14-15 0.7011849224452291 1102 16-17 0.4077189249854598 1102 18-19 0.19269145680247846 1102 20-21 0.5482314841613274 1102 22-23 0.2646424938663401 1102 24-25 0.5860106030747119 1102 26-27 0.3198137810276549 1102 28-29 0.5715497768994524 1102 30-31 1.2674130247411028 1102 32-33 0.5381502016836777 1102 34-35 0.22755577804350935 1102 36-37 0.5537553855386221 1102 38-39 0.6574468477813085 1102 40-41 1.167169420191243 1102 42-43 1.6280654267348211 1102 44-45 1.0311427096874937 1102 46-47 1.8864023835797852 1102 48-49 2.1500367030717555 1102 50-51 1.9662315800848589 1102 52-53 2.143038722446015 1102 54-55 2.625386939939915 1102 56-57 2.5978232281243265 1102 58-59 2.9387130128463923 1102 60-61 3.2859231928025885 1102 62-63 3.0817853531143875 1102 64-65 3.2696715657884887 1102 66-67 3.3120864005596538 1102 68-69 3.771845138876035 1102 70-71 3.802926827314696 1102 72-73 3.7550312703209876 1102 74-75 3.7116472513015957 1102 76-77 3.581806075011297 1102 78-79 3.916809891801787 1102 80-81 3.9936345233445074 1102 82-83 4.878058556269025 1102 84-85 4.536172358417325 1102 86-87 5.089703461709124 1102 88-89 4.806054560817763 1102 90-91 5.414625027380616 1102 92-93 4.631738283071144 1102 94-95 4.621809658726985 1102 96-97 4.809577013311447 1102 98-99 4.857532970093246 1102 100-101 3.777480012790246 1103 1 0.15157586313942772 1103 2 -0.001046205160029956 1103 3 0.34669960226106866 1103 4 0.20823638465428473 1103 5 0.17216642312830288 1103 6 0.01764817853703704 1103 7 0.30586562827579655 1103 8 0.3505001319270633 1103 9 0.6212075567808313 1103 10-11 0.5016644917956725 1103 12-13 0.3246585497042105 1103 14-15 -0.18864558602935233 1103 16-17 -0.09228107501454019 1103 18-19 0.0062507788363816985 1103 20-21 0.30246877229692615 1103 22-23 0.357862832849392 1103 24-25 0.0351631454475978 1103 26-27 0.48930530645138504 1103 28-29 0.012227743001147928 1103 30-31 0.41148082135127595 1103 32-33 -0.1482904762824262 1103 34-35 -0.12837642534632465 1103 36-37 -0.4547191907325612 1103 38-39 -1.020519253913605 1103 40-41 -1.290457698452819 1103 42-43 -1.2448159291973866 1103 44-45 -1.1807216970921672 1103 46-47 -0.08817388760665068 1103 48-49 0.2517316183259837 1103 50-51 -0.1608870639829334 1103 52-53 0.19388618007313596 1103 54-55 0.2609801602788906 1103 56-57 0.39443339761584895 1103 58-59 0.218374029795541 1103 60-61 -0.2818734173669064 1103 62-63 0.07331077684319709 1103 64-65 0.6510274979918762 1103 66-67 -0.2387610570674603 1103 68-69 -0.6688328272256534 1103 70-71 -1.0275816472615809 1103 72-73 -1.2534433059501957 1103 74-75 -0.923945969037387 1103 76-77 -0.4775159588870075 1103 78-79 -1.295054514977874 1103 80-81 -0.8961959851300705 1103 82-83 -1.2575346640699578 1103 84-85 -1.2180649297182704 1103 86-87 -0.5797880637146022 1103 88-89 -0.9227589985042712 1103 90-91 -0.22096819295836667 1103 92-93 -0.17334646269156906 1103 94-95 -0.2934445785611537 1103 96-97 -1.275168749400418 1103 98-99 -1.066195843466076 1103 100-101 -1.7055708346673804 1104 1 0.539219384189181 1104 2 0.5631965504441254 1104 3 0.6815820516869806 1104 4 0.3348082818658753 1104 5 0.23504231159193978 1104 6 0.32163943058733224 1104 7 0.4209558415070731 1104 8 0.47843889627916525 1104 9 -0.375785335510038 1104 10-11 0.44193239775520965 1104 12-13 0.4376984622247164 1104 14-15 0.8139514615376271 1104 16-17 0.5382547369045412 1104 18-19 0.8353923862721402 1104 20-21 0.8515393026413776 1104 22-23 0.39162445122008904 1104 24-25 0.7600127900621345 1104 26-27 0.7189389860577293 1104 28-29 0.34560663857249097 1104 30-31 0.654373112220604 1104 32-33 0.6611682443299287 1104 34-35 0.49341143687347255 1104 36-37 1.1022791690268647 1104 38-39 1.5464572359715802 1104 40-41 0.7997555328211021 1104 42-43 0.138863677144883 1104 44-45 0.8105303532085486 1104 46-47 1.3261235426831277 1104 48-49 0.4700203006660644 1104 50-51 -0.6975191033487036 1104 52-53 -1.149334158909916 1104 54-55 -0.9091947987150917 1104 56-57 -0.4632757330566477 1104 58-59 -0.9892530888485283 1104 60-61 -1.5779368400022271 1104 62-63 -1.4053660957647836 1104 64-65 -0.6667691121776151 1104 66-67 -0.6883236595824975 1104 68-69 -0.9110416845247293 1104 70-71 -0.8112065789182203 1104 72-73 -0.5600316055674739 1104 74-75 -1.8126829837995544 1104 76-77 -0.9276253082582464 1104 78-79 -0.7033377298493875 1104 80-81 -0.471647051286439 1104 82-83 -1.1908315476128806 1104 84-85 -1.1159599543218768 1104 86-87 -1.2811275935123057 1104 88-89 -1.4334205622002756 1104 90-91 -0.49147120006607636 1104 92-93 -0.6592677311442756 1104 94-95 -0.019114343460003624 1104 96-97 -0.4598325000838521 1104 98-99 -0.7397879703113475 1104 100-101 -0.29318701837432215 1105 1 0.17380425991630943 1105 2 0.02062648169742687 1105 3 -0.3662762243574349 1105 4 -0.44916845002201455 1105 5 -0.8289449967105398 1105 6 0.2618826881230305 1105 7 -0.047843735436345014 1105 8 0.1093220268978854 1105 9 0.23526701774220982 1105 10-11 0.21255640621634342 1105 12-13 0.12529761611154555 1105 14-15 0.0808737248903526 1105 16-17 0.5016339013677893 1105 18-19 0.2238112122901157 1105 20-21 0.24828705515327698 1105 22-23 0.4189909239858167 1105 24-25 0.2995421396126403 1105 26-27 0.4235926084797299 1105 28-29 0.4840254645904736 1105 30-31 -0.04961948428918106 1105 32-33 0.20347390271173538 1105 34-35 0.12627764522883922 1105 36-37 0.633082976536123 1105 38-39 0.4647544332217137 1105 40-41 1.0575556385852423 1105 42-43 1.5342893778323443 1105 44-45 0.10296821677279411 1105 46-47 -0.22821278730101113 1105 48-49 -0.8162039193233568 1105 50-51 -0.03543554967340157 1105 52-53 0.21541993945074012 1105 54-55 0.18665395855619238 1105 56-57 -0.009150931364423798 1105 58-59 0.14599281279081566 1105 60-61 1.2142366131971407 1105 62-63 0.7516936609776792 1105 64-65 1.255362035363369 1105 66-67 0.7134478898622412 1105 68-69 0.6807087120908193 1105 70-71 1.1135686722716223 1105 72-73 0.9787045128883669 1105 74-75 0.6978934307958973 1105 76-77 -0.30983049070139757 1105 78-79 -0.23837210319682 1105 80-81 -0.07027211738903105 1105 82-83 0.2821985951687189 1105 84-85 0.4494816109874833 1105 86-87 0.882145806804985 1105 88-89 0.4060823463137382 1105 90-91 0.5110406984003433 1105 92-93 1.1367396723459429 1105 94-95 0.5809649796494618 1105 96-97 -1.2582195968580443 1105 98-99 0.09759965528357739 1105 100-101 -0.9316058443923048 1106 1 0.07954196483434117 1106 2 -0.23065425600749023 1106 3 0.022811890396660317 1106 4 -0.04573607297283644 1106 5 -0.14298188195644457 1106 6 -0.12694927909008413 1106 7 0.14273003505545745 1106 8 -0.2866820714627707 1106 9 0.1156461161028659 1106 10-11 -0.12863211837382238 1106 12-13 -0.3574124248720594 1106 14-15 0.336380897021499 1106 16-17 -0.04818679535352288 1106 18-19 -0.4206560008246356 1106 20-21 -0.24851110058443027 1106 22-23 0.14414461251040933 1106 24-25 0.44247246748149394 1106 26-27 -0.5461819816842066 1106 28-29 -0.4026292485242777 1106 30-31 -0.7458285006826202 1106 32-33 -0.4507269169603916 1106 34-35 -0.5333022728039509 1106 36-37 -0.20644058903764773 1106 38-39 -0.6259217962864838 1106 40-41 -0.5276134187918018 1106 42-43 0.017895935209395475 1106 44-45 -0.6063043242108108 1106 46-47 0.03907717171538394 1106 48-49 -0.35936477997909577 1106 50-51 -0.2486783775422552 1106 52-53 -0.09464665890991597 1106 54-55 0.056531007736523975 1106 56-57 0.21011136371754446 1106 58-59 -0.28612808884852825 1106 60-61 -0.15832998516351537 1106 62-63 0.4358637429448926 1106 64-65 0.5910433878223849 1106 66-67 0.9598618242884704 1106 68-69 0.3437466219268863 1106 70-71 0.340003098501132 1106 72-73 -0.24324733137392585 1106 74-75 -0.14534427412213446 1106 76-77 0.08220332077400982 1106 78-79 -0.31624095565583943 1106 80-81 -0.26801801902837497 1106 82-83 0.22776724270970305 1106 84-85 0.48585456180715525 1106 86-87 -0.9439300128671455 1106 88-89 -1.0571604815551154 1106 90-91 -0.6290720065176885 1106 92-93 -1.4188443440474998 1106 94-95 -0.6786405531374236 1106 96-97 -1.0004070968580443 1106 98-99 -0.6407456316016678 1106 100-101 -1.86526363127755 1107 1 0.07196620725858338 1107 2 -0.1279080438862792 1107 3 0.16154295100272265 1107 4 -0.02442925479101632 1107 5 0.3200862998617353 1107 6 0.33043708454628273 1107 7 -0.16408814676272243 1107 8 0.28244671641601826 1107 9 0.41062717670892823 1107 10-11 0.5020496998079977 1107 12-13 0.6911197720976361 1107 14-15 1.107450972779077 1107 16-17 0.8251560076767817 1107 18-19 0.41457127190263776 1107 20-21 0.03629003577920997 1107 22-23 0.5631787034195028 1107 24-25 0.4017527705117985 1107 26-27 0.2274922607400356 1107 28-29 0.3111491605666288 1107 30-31 0.2039821053779889 1107 32-33 0.5199170224335461 1107 34-35 0.6461484847718069 1107 36-37 1.1702166079320477 1107 38-39 0.893964567349876 1107 40-41 -0.02453576727664597 1107 42-43 0.14668381399727082 1107 44-45 0.16879037275889175 1107 46-47 0.5194275505032593 1107 48-49 0.08121287153606005 1107 50-51 0.30174775882138505 1107 52-53 -0.05084931042506469 1107 54-55 -0.21051444680892928 1107 56-57 -0.15731287870669775 1107 58-59 -0.4665258161212549 1107 60-61 0.2434219087758791 1107 62-63 -0.6147991358429863 1107 64-65 -0.2652539606624664 1107 66-67 -0.5297215090448617 1107 68-69 -0.15175527201250816 1107 70-71 -0.6921938711958369 1107 72-73 -0.5138439222830158 1107 74-75 -0.20784427412213446 1107 76-77 -0.29492736104417006 1107 78-79 -0.672301561716445 1107 80-81 -0.28885135236171067 1107 82-83 -0.09870056032060148 1107 84-85 -1.1230469533443603 1107 86-87 -1.1347444068065364 1107 88-89 -1.0294616179187486 1107 90-91 0.12045450863382712 1107 92-93 0.11003823171007454 1107 94-95 0.38362270443833424 1107 96-97 -0.09912868776713779 1107 98-99 -0.48709979826833205 1107 100-101 -1.5769113585502765 1108 1 0.38691901401466566 1108 2 0.21734779317283426 1108 3 0.25667459531469206 1108 4 0.22296269751896602 1108 5 0.4005632000107653 1108 6 0.1799154750082792 1108 7 0.29641855964562325 1108 8 0.4371908793569048 1108 9 0.08772603413565605 1108 10-11 0.2453432914622411 1108 12-13 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8 42.0 9 87.0 10 167.0 11 265.0 12 407.0 13 555.0 14 605.0 15 862.0 16 1235.0 17 1552.0 18 1519.0 19 1042.0 20 1036.0 21 1174.0 22 1104.0 23 1081.0 24 1046.0 25 1146.0 26 1077.0 27 1064.0 28 1197.0 29 1486.0 30 1709.0 31 1977.0 32 2488.0 33 3264.0 34 4596.0 35 7207.0 36 9645.0 37 12914.0 38 4833.0 39 89.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.93272753920563 19.214421808588597 14.540524695881576 13.312325956324198 2 14.507560277891296 28.46926265397863 31.74441006480238 25.27876700332769 3 31.564889952711777 26.255181271527817 31.36493665713118 10.814992118629226 4 12.087687547434175 18.95761573938934 39.279876233288576 29.67482047988791 5 8.859244556016113 44.00577967190145 32.28880845349991 14.84616731858252 6 22.994628991768344 37.83495825792515 13.894564773191664 25.275847977114836 7 19.434876525191196 39.47691050265632 21.95983419931111 19.12837877284138 8 23.698114309066494 23.7141689532372 18.77955514040516 33.80816159729114 9 19.105026563138537 25.506451047930412 26.72952303111682 28.658999357814235 10-11 25.621752583338196 25.143762040983127 22.569180921244673 26.665304454434 12-13 34.37810146535116 24.89418529978399 23.35877751182206 17.368935723042792 14-15 25.42071079325695 28.009924833977962 32.03532073268627 14.534043640078814 16-17 16.16093061272149 31.257845111947923 31.90734740345039 20.6738768718802 18-19 15.55913947107245 34.77873781306556 31.572187518243915 18.089935197618072 20-21 20.832360324595715 26.375591102808105 28.454667522914356 24.337381049681824 22-23 16.679315780255706 27.530065969992414 32.57560277891295 23.215015470838928 24-25 24.39138303461965 27.9511355011968 28.990308832973323 18.667172631210228 26-27 21.454165967291118 23.30124719945704 38.97917928583418 16.265407547417663 28-29 15.416219687515964 35.26866183564303 32.201472659471214 17.113645817369793 30-31 17.68054177126511 28.125547317414913 31.139441882188102 23.054469029131884 32-33 17.977683881749385 29.54805846852172 34.48416781604163 17.990089833687268 34-35 21.569901105718355 27.000693354742182 31.960004379082584 19.469401160456883 36-37 18.550041241779017 26.060424680832426 38.103024153813585 17.286509923574968 38-39 18.27139947883592 29.47350749264604 35.03259100298538 17.22250202553266 40-41 16.46329003065246 31.7720040869946 33.06670559042475 18.698000291928185 42-43 15.656108597285067 31.820902058093708 28.583418479054153 23.93957086556707 44-45 19.337794741182396 26.07588285520372 30.686652995395068 23.89966940821882 46-47 23.028250731628997 30.864161491136528 31.078724009837767 15.028863767396713 48-49 20.1719369175418 28.39075510665781 32.474621789866234 18.962686185934157 50-51 12.800116762752682 31.21214332627892 37.659636575932275 18.328103335036122 52-53 17.536049973728765 33.74321326405511 28.167873197501315 20.55286356471481 54-55 14.430206083250626 27.950405744643586 31.15987506567809 26.459513106427696 56-57 21.029394593963456 26.038443575223308 28.906386829353725 24.02577500145951 58-59 25.454651604051726 26.00636366290101 28.656916834515574 19.882067898531687 60-61 18.920812382780287 29.35437024279532 33.80160404002073 17.923213334403666 62-63 15.307090521645211 29.531190705549232 32.96844440552296 22.1932743672826 64-65 14.695643924190124 35.39375451553344 28.80819108642822 21.10241047384822 66-67 18.700877903540075 27.767439484496208 25.981712167319802 27.54997044464391 68-69 15.703703703703702 28.01021711366539 29.82302499543879 26.46305418719212 70-71 22.623912662735712 27.404547842839627 26.6280868702201 23.343452624204566 72-73 19.724589876817095 22.438554498219393 35.35232646389165 22.48452916107187 74-75 15.233668048339075 32.174966431198555 28.907116585906937 23.684248934555434 76-77 17.32540557913267 25.048391927130886 29.49095344879221 28.13524904494423 78-79 18.3781613809715 25.869858764278675 31.779132148461738 23.972847706288093 80-81 21.20891470605406 23.992206200011676 29.682118045420047 25.116761048514213 82-83 17.503940685387352 23.341993111098137 34.40510245782007 24.748963745694436 84-85 17.006976472648724 26.520812656897657 31.46929184424076 25.002919026212854 86-87 16.81592403214025 27.283418553688826 29.70197224251278 26.19868517165814 88-89 16.2090105398853 26.54234631470237 26.404646631851108 30.843996513561223 90-91 18.96899060121459 21.976894957767733 28.27327536316819 30.780839077849485 92-93 20.78200712242396 25.672835542063165 29.135530387062875 24.409626948449997 94-95 18.62411699457061 23.54778445910444 29.96891237083309 27.859186175491857 96-97 13.318786852705939 25.283145542646974 33.569531204390216 27.828536400256876 98-99 17.812627707396814 26.464621402300192 26.654358106135795 29.0683927841672 100-101 16.67213988075691 22.481774197079492 28.38992636702644 32.45615955513716 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 851.0 1 524.5 2 223.0 3 227.0 4 171.5 5 150.5 6 247.0 7 420.5 8 565.5 9 606.5 10 450.0 11 267.5 12 223.5 13 230.0 14 219.5 15 216.0 16 217.0 17 200.0 18 208.5 19 222.5 20 239.0 21 259.5 22 271.0 23 302.5 24 358.0 25 404.0 26 459.0 27 499.5 28 540.0 29 601.0 30 685.5 31 758.0 32 753.5 33 822.5 34 928.5 35 950.0 36 963.0 37 985.5 38 908.5 39 730.5 40 614.0 41 578.0 42 556.0 43 594.5 44 614.5 45 734.0 46 1652.5 47 5341.5 48 11675.0 49 11149.0 50 6034.0 51 4029.0 52 2653.0 53 1457.5 54 602.5 55 343.5 56 276.0 57 244.0 58 210.5 59 167.0 60 116.5 61 80.0 62 61.0 63 45.5 64 38.5 65 31.0 66 22.0 67 24.0 68 21.0 69 14.5 70 17.0 71 14.5 72 9.5 73 8.0 74 5.5 75 7.0 76 8.0 77 7.0 78 8.0 79 8.5 80 4.5 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.417420748438321 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014595131064276958 16-17 0.0029190262128553916 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0036487827660692394 28-29 7.297565532138479E-4 30-31 0.0 32-33 7.297565532138479E-4 34-35 0.012405861404635413 36-37 0.025541479362484677 38-39 0.022622453149629282 40-41 0.008757078638566174 42-43 0.008757078638566174 44-45 0.0036487827660692394 46-47 0.006567808978924631 48-49 0.0036487827660692394 50-51 0.0014595131064276958 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0029190262128553916 60-61 0.0021892696596415434 62-63 0.0029190262128553916 64-65 0.0036487827660692394 66-67 7.297565532138479E-4 68-69 0.005108295872496935 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09413859536458638 78-79 0.019703426936773894 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.09632786502422792 88-89 0.3670675462665655 90-91 0.38312219043727014 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.297565532138479E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 68516.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.247416661801623 #Duplication Level Percentage of deduplicated Percentage of total 1 80.42265164823809 22.717321501547083 2 9.899762323033997 5.59285422383093 3 3.6788260824635732 3.117519995329558 4 1.896248837449623 2.1425652402358573 5 0.8938720677896042 1.262478837059957 6 0.6406944300919706 1.0858777511822058 7 0.4030174640901106 0.796894156109522 8 0.2531776376976336 0.5721291377196567 9 0.23251007543660224 0.5911028081032168 >10 1.2658881884881679 6.867009165742307 >50 0.10850470187041439 2.2564072625372176 >100 0.19634184147979744 12.207367622161247 >500 0.04133512452206262 9.186175491855916 >1k 0.06716957734835176 31.604296806585324 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4726 6.89765894097729 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2956 4.314320742600269 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1935 2.824157860937591 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1400 2.043318348998774 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1357 1.980559285422383 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1308 1.909043143207426 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1224 1.7864440422674994 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1223 1.7849845291610718 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1175 1.7149279000525424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1122 1.6375737054118746 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1116 1.6288166267733086 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1084 1.5821122073676221 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1028 1.5003794734076712 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 977 1.4259443049798588 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 888 1.296047638507794 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 885 1.2916690991885107 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 758 1.1063109346721933 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 715 1.0435518710958025 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 701 1.0231186876058147 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 694 1.012902095860821 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 676 0.9866308599451223 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 492 0.7180804483624262 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 492 0.7180804483624262 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 478 0.6976472648724386 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 432 0.6305096619767646 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 403 0.5881837818903614 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 397 0.5794267032517952 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 333 0.48601786444042266 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 290 0.4232588008640317 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 284 0.41450172222546555 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 280 0.4086636697997548 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 262 0.38239243388405625 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 242 0.3532021717555024 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 231 0.3371475275847977 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 216 0.3152548309883823 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 212 0.30941677856267147 RNA PCR Primer, Index 27 (95% over 23bp) CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 211 0.3079572654562438 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 198 0.28898359507268373 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 195 0.28460505575340067 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 183 0.2670908984762683 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 181 0.26417187226341293 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 179 0.26125284605055754 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 171 0.24957674119913598 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 163 0.2379006363477144 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148 0.21600793975129895 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.21162940043201586 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 144 0.2101698873255882 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 141 0.2057913480063051 RNA PCR Primer, Index 27 (96% over 30bp) ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 134 0.1955747562613112 RNA PCR Primer, Index 27 (96% over 25bp) GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 132 0.19265573004845585 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 127 0.18535816451631734 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 119 0.1736820596648958 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 112 0.1634654679199019 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 111 0.16200595481347424 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 110 0.16054644170704652 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 108 0.15762741549419115 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 104 0.15178936306848037 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 103 0.15032984996205265 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 101 0.14741082374919728 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 100 0.1459513106427696 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 97 0.1415727713234865 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 96 0.14011325821705878 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 94 0.13719423200420341 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 90 0.13135617957849263 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 89 0.1298966664720649 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 84 0.12259910093992644 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 80 0.11676104851421566 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 80 0.11676104851421566 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 75 0.10946348298207717 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 74 0.10800396987564947 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 73 0.1065444567692218 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0036487827660692394 0.0 22-23 0.0 0.0 0.0 0.008757078638566174 0.0 24-25 0.0 0.0 0.0 0.02043318348998774 0.0 26-27 0.0 0.0 0.0 0.048163932512113955 0.0 28-29 0.0 0.0 0.0 0.11238250919493256 0.0 30-31 0.0 0.0 0.0 0.2700099246891237 0.0 32-33 0.0 0.0 0.0 0.5349115535057505 0.0 34-35 0.0 0.0 0.0 0.9151147177301653 0.0 36-37 0.0 0.0 0.0 1.5164341175783758 0.0 38-39 0.0 0.0 0.0 2.176134041683694 0.0 40-41 0.0 0.0 0.0 2.7686963628933388 0.0 42-43 0.0 0.0 0.0 3.320392317123008 0.0 44-45 0.0 0.0 0.0 3.9217117169712186 0.0 46-47 0.0 0.0 0.0 4.624467277716154 0.0 48-49 0.0 0.0 0.0 5.259355479012202 0.0 50-51 0.0 0.0 0.0 5.875999766477903 0.0 52-53 0.0 0.0 0.0 6.377342518535816 0.0 54-55 0.0 0.0 0.0 6.999824858427228 0.0 56-57 0.0 0.0 0.0 7.844153190495651 0.0 58-59 0.0 0.0 0.0 8.458608208301712 0.0 60-61 0.0 0.0 0.0 9.062846634362778 0.0 62-63 0.0 0.0 0.0 9.629137719656722 0.0 64-65 0.0 0.0 0.0 10.240673711249928 0.0 66-67 0.0 0.0 0.0 10.965321968591278 0.0 68-69 0.0 0.0 0.0 11.821326405511122 0.0 70-71 0.0 0.0 0.0 12.67076303345204 0.0 72-73 0.0 0.0 0.0 13.336301009983071 0.0 74-75 0.0 0.0 0.0 14.210549360733259 0.0 76-77 0.0 0.0 0.0 14.919142973903906 0.0 78-79 0.0 0.0 0.0 16.130538852238892 0.0 80-81 0.0 0.0 0.0 17.325880086403174 0.0 82-83 0.0 0.0 0.0 18.17239768813124 0.0 84-85 0.0 0.0 0.0 19.175083192247065 0.0 86-87 0.0 0.0 0.0 20.140551112148987 0.0 88-89 0.0 0.0 0.0 21.25124058614046 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGAAC 20 0.002072042 71.19526 3 CACGAGC 25 2.8266839E-5 47.4635 94-95 GTAAGCG 65 0.0 43.81247 94-95 TCAACGA 35 4.3050804E-6 40.683002 94-95 CTCCGAC 45 5.683378E-7 36.916058 94-95 CCGCAGA 30 0.0038557844 31.804844 88-89 TCACCGC 30 0.0038557844 31.804844 84-85 CATGGGG 135 4.1560088E-7 28.126522 4 CACCGCA 35 0.008190286 27.261292 86-87 CAGGTAC 35 0.008397674 27.122002 20-21 AAGCAGG 45 8.9472777E-4 26.368612 94-95 GTACTTT 940 0.0 23.22682 14-15 CATGGGC 185 2.488414E-7 23.090353 4 GAGTACT 950 0.0 22.982328 12-13 ACTTTTT 1020 0.0 21.405111 16-17 GCTTGAT 135 3.7653995E-4 21.094893 9 GGGCTTG 135 3.7653995E-4 21.094893 7 GGCTTGA 140 4.8128678E-4 20.341501 8 TGGGCTT 145 6.0970127E-4 19.64007 6 ATGGGCT 150 7.6597376E-4 18.985401 5 >>END_MODULE