FastQCFastQC Report
Fri 27 May 2016
SRR939107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966724
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121440.6174735245006416No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66180.33649866478468765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63550.3231261732708809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50720.2578907869126527No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48020.24416237357148232No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43070.2189936157793366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32380.16463926814336938No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32140.16341896473526535No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27330.13896205059784697No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25550.12991146698774206No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24040.12223372471175417No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23750.1207591914269618No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20450.10398001956553131No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG80650.025.8401761
TACATGG81400.024.8521732
ACATGGG84300.022.9294433
GAGTACT58500.020.68273712-13
GGTCGAC1404.953117E-420.295857
TACCTGG16550.020.0383012
CATGGGG47600.019.6074074
GTACTTT63100.019.28925314-15
AGTACTT60200.018.64338712-13
GAGTACA66900.018.3074951
AGTACAT63000.018.0481032
CATGGGA39200.018.0078184
GTACACG3701.4370016E-1017.9622861
AGAGTAC103550.017.92705310-11
GCACCGT7950.017.870566
GTACCTG19800.017.2624591
ACTTTTT70550.017.18738716-17
GTATAGG4255.820766E-1116.754741
TACTTTT67500.016.27780514-15
TATAGCG2053.4004383E-416.1772635
Produced by FastQC (version 0.11.5)