FastQCFastQC Report
Fri 27 May 2016
SRR939107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966724
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT130580.6639467459592704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64860.32978699604011547No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60990.3101096035844379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55150.28041555398723966No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44970.22865435109349352No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42020.21365478836888144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35330.17963883086798146No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34280.17430000345752633No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28590.14536864349039316No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27510.139877278153925No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26470.13458929671880754No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24980.12701324639349498No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22980.11684405132596135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG83700.027.5926741
TACATGG83100.026.7780322
ACATGGG85300.025.4755333
CATGGGG47550.021.5531144
TACCTGG15700.021.154622
GAGTACT65250.020.43354412-13
GTATAGG3603.6379788E-1219.8411241
CATGGGA42650.019.801944
GTACTTT68450.019.3049914-15
TACGCTA1751.0027218E-418.9787169
AGTACTT67950.018.5043712-13
AGAGTAC112500.018.03023710-11
GTACCTG19450.017.8723131
ATGGGAG20200.017.6163835
CCGACTA1650.001475347717.2533789
GTAAGGT6600.017.2533784
ACTTTTT77850.017.21758316-17
ATTCGCG3600.017.15384180-81
ATGGGGA28350.017.07085
CGTACAC1952.2959174E-417.032183
Produced by FastQC (version 0.11.5)