##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939107_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1966724 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50893923092411 34.0 31.0 34.0 31.0 34.0 2 32.71459238815411 34.0 31.0 34.0 31.0 34.0 3 32.80852575145267 34.0 31.0 34.0 31.0 34.0 4 36.258949400119185 37.0 35.0 37.0 35.0 37.0 5 36.21663080330539 37.0 37.0 37.0 35.0 37.0 6 36.2615349179651 37.0 37.0 37.0 35.0 37.0 7 36.21141248085649 37.0 37.0 37.0 35.0 37.0 8 36.200296025268415 37.0 37.0 37.0 35.0 37.0 9 37.94241744138984 39.0 38.0 39.0 35.0 39.0 10-11 37.881443201994784 39.0 38.0 39.0 35.0 39.0 12-13 37.81618468071778 39.0 38.0 39.0 35.0 39.0 14-15 39.17895368135031 40.5 39.0 41.0 36.0 41.0 16-17 39.127090532276014 40.0 38.0 41.0 36.0 41.0 18-19 39.14320794376842 40.5 38.5 41.0 36.0 41.0 20-21 39.20486733268115 41.0 39.0 41.0 36.0 41.0 22-23 39.16209595245698 41.0 39.0 41.0 36.0 41.0 24-25 39.09165139592541 41.0 39.0 41.0 35.0 41.0 26-27 38.94933656171379 40.0 38.0 41.0 35.0 41.0 28-29 38.80313201038885 40.0 38.0 41.0 35.0 41.0 30-31 38.626166915134 40.0 38.0 41.0 34.5 41.0 32-33 38.484848407809125 40.0 38.0 41.0 34.0 41.0 34-35 38.43151758965671 40.0 38.0 41.0 34.0 41.0 36-37 38.32175917922393 40.0 38.0 41.0 34.0 41.0 38-39 38.18372481344612 40.0 38.0 41.0 34.0 41.0 40-41 38.020558044748526 40.0 38.0 41.0 33.0 41.0 42-43 37.84704996735688 40.0 37.0 41.0 33.0 41.0 44-45 37.61755182730266 40.0 37.0 41.0 33.0 41.0 46-47 37.324049790412886 40.0 37.0 41.0 32.5 41.0 48-49 36.99577266561043 40.0 36.0 41.0 31.5 41.0 50-51 36.71874828394833 39.5 36.0 41.0 31.0 41.0 52-53 36.47648882100386 39.0 35.0 41.0 31.0 41.0 54-55 36.2085409543993 39.0 35.0 41.0 30.0 41.0 56-57 35.8914240127237 38.5 35.0 40.0 29.5 41.0 58-59 35.56018104217979 38.0 35.0 40.0 29.0 41.0 60-61 35.14130706698042 37.5 34.5 40.0 28.0 41.0 62-63 34.73928522761709 37.0 34.0 40.0 28.0 41.0 64-65 34.30222491818883 36.5 34.0 39.0 27.0 41.0 66-67 33.90821360801007 36.0 33.5 39.0 26.0 40.0 68-69 33.42329172776658 35.0 33.0 38.5 26.0 40.0 70-71 32.96250109318847 35.0 33.0 37.5 25.5 40.0 72-73 32.49196074283936 35.0 32.0 37.0 24.5 39.0 74-75 32.01180338471488 35.0 32.0 36.5 23.5 39.0 76-77 30.08062214118504 33.5 29.5 35.0 20.0 36.5 78-79 31.07175434885627 34.0 31.0 35.0 20.5 37.0 80-81 31.114972410973785 34.5 31.0 35.0 20.5 37.0 82-83 30.850624185193247 34.0 31.0 35.0 20.0 36.5 84-85 30.507748672411584 34.0 31.0 35.0 18.5 36.0 86-87 30.00276068223096 34.0 30.5 35.0 14.0 36.0 88-89 29.482349073891406 34.0 30.0 35.0 7.0 35.0 90-91 29.0397043509918 34.0 29.0 35.0 2.0 35.0 92-93 28.766003262277778 34.0 29.0 35.0 2.0 35.0 94-95 28.43911219876302 34.0 29.0 35.0 2.0 35.0 96-97 27.86807528661876 34.0 29.0 35.0 2.0 35.0 98-99 27.10418442038639 33.0 27.0 35.0 2.0 35.0 100-101 23.434502248409032 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21380490076737146 1101 2 0.22145748962640255 1101 3 0.1765208872838997 1101 4 0.15190473122800796 1101 5 0.15665303649134188 1101 6 0.16531074743288343 1101 7 0.11877160903092943 1101 8 0.17974360366789455 1101 9 0.2157618755135715 1101 10-11 0.1625717116803358 1101 12-13 0.2393933594386013 1101 14-15 0.3253871804263966 1101 16-17 0.3422466376041413 1101 18-19 0.32578940426935077 1101 20-21 0.34037167884277153 1101 22-23 0.30406099604690695 1101 24-25 0.286373493698008 1101 26-27 0.25184047796496856 1101 28-29 0.18290025291182843 1101 30-31 0.17171195867283728 1101 32-33 0.35294800698601847 1101 34-35 0.4425858712969273 1101 36-37 0.28067395497861725 1101 38-39 0.3665294804944992 1101 40-41 0.4016686871838573 1101 42-43 0.37059463800001424 1101 44-45 0.42589801008791284 1101 46-47 0.3553931189669868 1101 48-49 0.4427128392670241 1101 50-51 0.4032956764838005 1101 52-53 0.43484858511505564 1101 54-55 0.4135949962903993 1101 56-57 0.3847463958467614 1101 58-59 0.5342375761018658 1101 60-61 0.6053445472251653 1101 62-63 0.5399970333953732 1101 64-65 0.42919787909138307 1101 66-67 0.3846838750799506 1101 68-69 0.21944118001535884 1101 70-71 0.30411246079653864 1101 72-73 0.2933342575966691 1101 74-75 0.3422751286846122 1101 76-77 0.4370152421418787 1101 78-79 0.6361466156741642 1101 80-81 0.37601329575414866 1101 82-83 0.5107957852046887 1101 84-85 0.4357947205695005 1101 86-87 0.4940464845619026 1101 88-89 0.8015132804367049 1101 90-91 0.8971194604859605 1101 92-93 0.7254389430070418 1101 94-95 0.47529648837099714 1101 96-97 0.4274922357716271 1101 98-99 0.6125176407397035 1101 100-101 0.348757444878391 1102 1 0.1590770390854388 1102 2 0.15344870032211588 1102 3 0.19322159703053643 1102 4 0.12164971761077936 1102 5 0.10077080491587509 1102 6 0.13328652526568163 1102 7 0.11306371928924364 1102 8 0.14322260011915944 1102 9 0.2295297223403452 1102 10-11 0.181527001768643 1102 12-13 0.2203338768934202 1102 14-15 0.2635185248185721 1102 16-17 0.30677890631751836 1102 18-19 0.22796506656199256 1102 20-21 0.24967730317222703 1102 22-23 0.22846988438561056 1102 24-25 0.11837244145735326 1102 26-27 0.3144910928036282 1102 28-29 0.16498998221776873 1102 30-31 0.21525821599725248 1102 32-33 0.33839820092841677 1102 34-35 0.2505921847408459 1102 36-37 0.38205269971741274 1102 38-39 0.43194480607013475 1102 40-41 0.31470925002944483 1102 42-43 0.27400874627763727 1102 44-45 0.2615375041871033 1102 46-47 0.3374111514832876 1102 48-49 0.3735037734916702 1102 50-51 0.46961778591682446 1102 52-53 0.5031859417214761 1102 54-55 0.4018047429273537 1102 56-57 0.32175122377446996 1102 58-59 0.4326169191735758 1102 60-61 0.3888847180595292 1102 62-63 0.39593084539503565 1102 64-65 0.3956241809812866 1102 66-67 0.5518843991370588 1102 68-69 0.4383278681394813 1102 70-71 0.40002935441706455 1102 72-73 0.11146374509357315 1102 74-75 0.3037589943279215 1102 76-77 0.30434265404251093 1102 78-79 0.4209303905359292 1102 80-81 0.37006762025691486 1102 82-83 0.35664201364407333 1102 84-85 0.40302309805568015 1102 86-87 0.41977428434733 1102 88-89 0.43855111983589623 1102 90-91 0.663947573880364 1102 92-93 0.6188500596200655 1102 94-95 0.36334633569294184 1102 96-97 0.42915364123502187 1102 98-99 0.3880654248446831 1102 100-101 -0.008910500982796776 1103 1 0.12766040018333769 1103 2 0.1035219098040514 1103 3 0.1473535415093039 1103 4 0.13445437401723837 1103 5 0.13255489181419478 1103 6 0.12075209967257905 1103 7 0.16406912621236103 1103 8 0.16266471849146313 1103 9 0.1158630625942223 1103 10-11 0.22459141260120674 1103 12-13 0.24526102167748576 1103 14-15 0.21129806788463412 1103 16-17 0.1414109953038647 1103 18-19 0.19887669680187514 1103 20-21 0.1411623302430911 1103 22-23 0.23487517853678952 1103 24-25 0.16869716332558227 1103 26-27 0.20891372654563867 1103 28-29 0.19736066771094585 1103 30-31 0.27923783268473557 1103 32-33 0.26198285880467154 1103 34-35 0.18785282862178576 1103 36-37 0.2922230341082681 1103 38-39 0.340675502280277 1103 40-41 0.24469458836006908 1103 42-43 0.3452780039305239 1103 44-45 0.3543334472975843 1103 46-47 0.4685464207897141 1103 48-49 0.5275481895508918 1103 50-51 0.4910126514654607 1103 52-53 0.6477867795029084 1103 54-55 0.6377613913138873 1103 56-57 0.707517903194713 1103 58-59 0.7936965308189414 1103 60-61 0.6505162596637035 1103 62-63 0.7281547494196019 1103 64-65 0.5303357244177676 1103 66-67 0.520769463051586 1103 68-69 0.5032200081467195 1103 70-71 0.5320896699346136 1103 72-73 0.38057178238410927 1103 74-75 0.3766977511277716 1103 76-77 0.28709962130141164 1103 78-79 0.3541273561734428 1103 80-81 0.3661555349087955 1103 82-83 0.46668280201939893 1103 84-85 0.5622488604114046 1103 86-87 0.5313130412130853 1103 88-89 0.4218617307124255 1103 90-91 0.4852787045030169 1103 92-93 0.521615904949833 1103 94-95 0.6086325381360282 1103 96-97 0.6687027116904609 1103 98-99 0.5660208189647875 1103 100-101 0.09265871616842247 1104 1 0.12296781790273315 1104 2 0.13730394631931375 1104 3 0.15786228210954079 1104 4 0.023633265161265626 1104 5 0.11620496749996789 1104 6 0.10394498065372915 1104 7 0.08369943055323859 1104 8 0.1342711627230102 1104 9 0.153547777275854 1104 10-11 0.14480596364884235 1104 12-13 0.11528152966732819 1104 14-15 0.1394901050608155 1104 16-17 0.1387830080578354 1104 18-19 0.140389141802153 1104 20-21 0.1383633361356118 1104 22-23 0.19089545423963017 1104 24-25 0.18317467480036953 1104 26-27 0.15782566731629544 1104 28-29 0.21530323378922134 1104 30-31 0.26681882031513027 1104 32-33 0.23688126532872644 1104 34-35 0.2496677985560254 1104 36-37 0.2658047198830005 1104 38-39 0.3009173695083618 1104 40-41 0.44120280791876354 1104 42-43 0.3054395011421178 1104 44-45 0.2039281000766593 1104 46-47 0.24102772714096687 1104 48-49 0.17685466528877214 1104 50-51 0.08200771247929595 1104 52-53 0.09789929752600557 1104 54-55 0.19809537124352516 1104 56-57 0.13767571718159388 1104 58-59 0.18170195555442348 1104 60-61 0.1409065082300387 1104 62-63 0.17473644096941854 1104 64-65 0.20134354838272372 1104 66-67 0.3091425364972693 1104 68-69 0.08727020138768893 1104 70-71 -0.0151695039578712 1104 72-73 -0.06820492500551012 1104 74-75 -0.08055951698467823 1104 76-77 0.14196228001214095 1104 78-79 0.313583811024742 1104 80-81 0.027360783568170177 1104 82-83 0.10272726252002329 1104 84-85 0.013445170513257665 1104 86-87 0.10544740319707202 1104 88-89 0.2622791222064862 1104 90-91 0.32357006041096525 1104 92-93 0.2621234199474216 1104 94-95 0.19230061285543698 1104 96-97 0.3179379613059332 1104 98-99 0.30381404118964994 1104 100-101 0.021803001683789347 1105 1 -0.07761113933288044 1105 2 0.03638543448855813 1105 3 -0.002348160335152727 1105 4 -0.02317358957399307 1105 5 0.02632722195500037 1105 6 -0.020732485649823218 1105 7 0.027496546374287334 1105 8 -0.03082970460278034 1105 9 0.009135935663948658 1105 10-11 0.0507765988983806 1105 12-13 -0.02446159043608276 1105 14-15 0.013482418521633122 1105 16-17 0.032973454646743505 1105 18-19 0.04237775013400835 1105 20-21 -2.3765699683053754E-4 1105 22-23 -0.06741456786286903 1105 24-25 -0.10137713286840722 1105 26-27 -0.024490975669117176 1105 28-29 -0.003636401223509722 1105 30-31 -0.06970408142741746 1105 32-33 -0.05723369727463279 1105 34-35 0.08197340262524477 1105 36-37 -0.09060580692614906 1105 38-39 0.03623499427895638 1105 40-41 0.08235477740942088 1105 42-43 0.003136931233093776 1105 44-45 -0.015290899686526416 1105 46-47 -0.04390982589517023 1105 48-49 -0.058720431409668095 1105 50-51 0.022734899541440257 1105 52-53 -0.0829850615014891 1105 54-55 0.01022720180418446 1105 56-57 -0.12441713798781961 1105 58-59 0.022129180111882363 1105 60-61 0.006895299104861863 1105 62-63 -0.12620754053946115 1105 64-65 -0.12417774747503074 1105 66-67 -0.1798524657721785 1105 68-69 -0.18034735381922928 1105 70-71 -0.022414794842568142 1105 72-73 0.039504370378622866 1105 74-75 -0.13307731492440666 1105 76-77 -0.11013388066514196 1105 78-79 0.11244674098744412 1105 80-81 0.017750451142621415 1105 82-83 0.0040288679114546255 1105 84-85 -0.08456868795180128 1105 86-87 -0.011569116941856805 1105 88-89 0.13983564885775834 1105 90-91 0.2217200243957329 1105 92-93 0.1560937049118003 1105 94-95 -0.08258258431070686 1105 96-97 -0.3044626514464177 1105 98-99 -0.12017346201718482 1105 100-101 -0.5227729435927877 1106 1 0.01728583275473028 1106 2 5.757215015904649E-4 1106 3 0.04679084751638385 1106 4 0.04656160896839623 1106 5 0.028848916984870243 1106 6 0.011832364645080418 1106 7 0.10436480776652957 1106 8 0.10826149904874427 1106 9 0.06651388938121983 1106 10-11 0.06669567925451503 1106 12-13 0.08724374691693271 1106 14-15 0.048502047358184086 1106 16-17 -0.02844648975386832 1106 18-19 0.08904906573564375 1106 20-21 0.07018138109626193 1106 22-23 0.05650185461731638 1106 24-25 0.11468681581486351 1106 26-27 0.12714765651090687 1106 28-29 0.04181926484208276 1106 30-31 0.040226795014227434 1106 32-33 0.18178965070989506 1106 34-35 -0.04358599573682653 1106 36-37 0.10016194824873281 1106 38-39 0.16868737791470778 1106 40-41 0.09908507343857309 1106 42-43 0.14595726163619105 1106 44-45 0.20313684459387105 1106 46-47 0.29952613619957447 1106 48-49 0.25530397315097986 1106 50-51 0.1309646884140463 1106 52-53 0.20329013196934653 1106 54-55 0.14191532870601975 1106 56-57 0.207290242079047 1106 58-59 0.11447745577964241 1106 60-61 0.1426488961595993 1106 62-63 0.10652859248581592 1106 64-65 0.3242537065615565 1106 66-67 0.24900591545927142 1106 68-69 0.0820903081891089 1106 70-71 0.06034270450000889 1106 72-73 -0.043119295987509076 1106 74-75 -0.06485243326093837 1106 76-77 0.07872205870147653 1106 78-79 0.21943916181467316 1106 80-81 0.20286082812898343 1106 82-83 0.2547344259714883 1106 84-85 0.34139467468390805 1106 86-87 0.3166075378911586 1106 88-89 0.5044665281169323 1106 90-91 0.7158554398884291 1106 92-93 0.5664033051973121 1106 94-95 0.3532280678550386 1106 96-97 0.47826655513534533 1106 98-99 0.3698019784576658 1106 100-101 -0.15542196778603667 1107 1 -0.049460181934122716 1107 2 -0.0644303941661093 1107 3 0.008156087343955676 1107 4 0.013992162101565953 1107 5 0.05245978273161711 1107 6 0.06541928533043517 1107 7 0.04510753225991948 1107 8 0.05394036066812191 1107 9 0.06542719093033611 1107 10-11 0.09813257422819532 1107 12-13 0.09779847792319885 1107 14-15 0.10486225737257371 1107 16-17 0.1403414377943193 1107 18-19 0.08277305164316573 1107 20-21 0.11207159501523734 1107 22-23 0.06610182131027642 1107 24-25 0.017066103111716302 1107 26-27 0.13350257790741438 1107 28-29 0.06095041806459989 1107 30-31 0.12632872918555194 1107 32-33 0.10996825071146787 1107 34-35 0.15865792544701662 1107 36-37 0.1149605137955163 1107 38-39 0.2373440464680172 1107 40-41 0.23936073983255568 1107 42-43 0.08779132618658281 1107 44-45 0.07108371311320383 1107 46-47 0.09326033364055775 1107 48-49 0.040318280550209806 1107 50-51 -0.06771813629302414 1107 52-53 -0.008650245052848504 1107 54-55 -0.053965935071111915 1107 56-57 0.04247510740495386 1107 58-59 -0.05772655321776199 1107 60-61 -0.08096919924032164 1107 62-63 -0.13619969858170577 1107 64-65 -0.10060938012338738 1107 66-67 -0.06521900383304313 1107 68-69 -0.09996051655397054 1107 70-71 -0.11828388081946173 1107 72-73 -0.15650121521568394 1107 74-75 -0.20318692371385794 1107 76-77 -0.1045583696990775 1107 78-79 -0.02291864469167848 1107 80-81 -0.0901042692434757 1107 82-83 -0.12510789444899117 1107 84-85 -0.08101848789833 1107 86-87 -0.17947761898387782 1107 88-89 -0.24546736415954484 1107 90-91 0.005716006040596966 1107 92-93 0.015170382088189882 1107 94-95 0.019599969090666747 1107 96-97 0.1075054667602906 1107 98-99 0.4051054719836067 1107 100-101 0.11872873081788171 1108 1 -0.024534427769793865 1108 2 0.028176400212217345 1108 3 0.03136415552644678 1108 4 7.191176928955656E-5 1108 5 0.09811415331217432 1108 6 0.07280903451749765 1108 7 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0.0234102540691552 2316 84-85 -0.07558857206378988 2316 86-87 -0.20439928263386165 2316 88-89 -0.4289557875323631 2316 90-91 -0.4006903685738692 2316 92-93 -0.42160521653712024 2316 94-95 -0.1364251233506124 2316 96-97 -0.3528267507973588 2316 98-99 -0.2203832546611899 2316 100-101 -0.03224072361977903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 9.0 5 43.0 6 79.0 7 198.0 8 446.0 9 840.0 10 1471.0 11 2189.0 12 3451.0 13 4870.0 14 5809.0 15 7795.0 16 10299.0 17 14646.0 18 14663.0 19 10114.0 20 9783.0 21 10372.0 22 10828.0 23 11612.0 24 13588.0 25 15522.0 26 18832.0 27 22560.0 28 27386.0 29 33447.0 30 41264.0 31 50852.0 32 64071.0 33 84377.0 34 117814.0 35 178519.0 36 295710.0 37 483835.0 38 370234.0 39 29188.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.67593206778526 18.217289609946956 13.690176116777117 23.416602205490662 2 16.36945499215955 20.233952501723678 38.4024397932806 24.99415271283617 3 20.917780024039978 23.16690089712639 28.5240328587031 27.391286220130535 4 13.75292110128315 15.560444678561913 34.95503181941137 35.731602400743576 5 14.736333110288987 36.80724900901194 32.79865400534086 15.65776387535821 6 34.12980163968101 34.265357009931236 16.141258254844097 15.463583095543656 7 30.74056146159807 31.454337263388254 19.34623261830333 18.458868656710347 8 27.245510808837437 33.93557001389112 18.576882165469076 20.24203701180237 9 26.600071997901075 17.09355252694328 19.816507044201423 36.48986843095422 10-11 26.537836524087773 25.315880621785265 25.688937542837735 22.45734531128923 12-13 27.56223038921577 22.53170755022057 26.562929012916914 23.343133047646745 14-15 23.632159092613605 24.290545066910187 25.3103225778568 26.766973262619402 16-17 23.391248792068495 27.54335523902235 25.7324939867193 23.332901982189856 18-19 23.347257507219496 26.374195457571947 27.492069932555342 22.78647710265322 20-21 24.08291656582215 25.04970194089257 27.385591470892713 23.481790022392566 22-23 23.641751460804873 24.919688781954154 27.665371450188232 23.77318830705274 24-25 24.114679437865234 25.162872950200764 27.509016294667717 23.213431317266288 26-27 23.52814359626757 25.07789029233361 28.24175519656319 23.152210914835624 28-29 23.04454005747629 26.20596484305881 27.760834768884703 22.988660330580192 30-31 23.302091741719792 25.246680607374167 27.496990459725552 23.95423719118049 32-33 23.334235001962654 25.52048482654404 27.69966706055349 23.445613110939817 34-35 24.14599802899152 25.265470840160166 27.426816902669 23.16171422817932 36-37 23.93455946601078 24.941409289679186 27.741607847086385 23.382423397223658 38-39 23.403343683544435 25.54393279372549 27.83114212473195 23.221581397998122 40-41 23.439025580941223 25.63156508331706 27.569434273904836 23.35997506183688 42-43 23.31686433364878 25.71139518836037 26.842782921938873 24.128957556051976 44-45 23.58756196732381 25.26540535826831 27.09866441174549 24.048368262662382 46-47 24.121486569146434 26.020298947019455 26.700955863696755 23.15725862013736 48-49 24.082796099681143 25.790749123790942 26.59928174500473 23.527173031523187 50-51 23.238447092334027 26.129499139050306 27.162791312556138 23.469262456059532 52-53 24.03446035132535 26.321181823173966 26.17614367852327 23.46821414697741 54-55 23.358005495433016 25.67520912949656 26.588250308635068 24.37853506643535 56-57 23.795001230472604 25.83397568748843 26.4759823950895 23.895040686949464 58-59 24.490196995691132 25.419242546431935 26.297617970123692 23.792942487753237 60-61 23.74768056033696 25.78080386223253 27.21825895447364 23.253256622956872 62-63 23.332671460456826 25.966374654455326 27.09492299435357 23.60603089073428 64-65 23.073457657661322 26.393818223974975 26.592096137457773 23.940627980905933 66-67 23.424975891677423 25.919783672137996 26.285813221454106 24.36942721473047 68-69 23.12342927893972 25.836084412472466 26.767845361597942 24.272640946989874 70-71 23.665705931718414 25.756238739202537 26.506293335082436 24.071761993996617 72-73 23.569702713751393 25.508993636117726 26.960595386032814 23.960708264098063 74-75 23.00505815762659 26.705679088677414 26.221930479314842 24.067332274381155 76-77 23.418106920812477 25.81728488419791 26.336790418880955 24.427817776108657 78-79 23.422753033225913 25.93523830055961 26.560747397440814 24.081261268773662 80-81 23.619552511030108 26.067404532449128 26.391049203651058 23.921993752869707 82-83 23.477328837176074 25.856039749501264 26.62023225004722 24.04639916327544 84-85 23.1736634118463 26.074655111749284 26.44244947435431 24.30923200205011 86-87 23.179779270010574 26.131371628319506 26.404681827378614 24.284167274291306 88-89 22.959435797168776 26.14974449555101 26.003844052948892 24.886975654331323 90-91 23.20315283447823 25.716671617854086 26.22835386800032 24.851821679667363 92-93 23.424995067940394 25.907753197703386 26.29237757814518 24.37487415621104 94-95 23.13151209829137 25.477265747507023 26.64359615279012 24.747626001411486 96-97 22.745749466053567 25.495424242401132 26.97478827094912 24.784038020596185 98-99 22.759747556564353 25.913968489315593 26.152515536193157 25.1737684179269 100-101 22.793065576521197 24.830147810302485 26.79243508193887 25.58435153123745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 910.0 1 622.5 2 489.0 3 648.0 4 512.0 5 455.5 6 827.5 7 1604.5 8 2414.0 9 2690.5 10 2307.5 11 1910.0 12 1884.5 13 1988.0 14 2154.5 15 2219.5 16 2245.5 17 2325.0 18 2383.0 19 2511.5 20 2781.0 21 3111.5 22 3562.0 23 4264.0 24 4979.5 25 5865.0 26 7155.0 27 8687.0 28 10113.5 29 11934.5 30 14531.5 31 17414.0 32 20816.5 33 23209.0 34 24796.0 35 26897.5 36 30872.0 37 35045.0 38 37711.0 39 39508.5 40 40996.0 41 44740.0 42 49016.5 43 52734.0 44 56939.5 45 61867.5 46 64924.5 47 74832.0 48 96399.0 49 97383.0 50 81838.0 51 76349.0 52 75994.5 53 78717.0 54 80308.0 55 76562.0 56 70775.0 57 65616.0 58 61479.5 59 55985.5 60 48237.0 61 40667.5 62 34151.0 63 27287.5 64 20748.5 65 15792.0 66 11832.5 67 9672.5 68 7607.0 69 5295.5 70 3902.5 71 2967.0 72 2328.5 73 2033.5 74 1631.0 75 1221.0 76 815.0 77 443.5 78 272.5 79 184.0 80 112.0 81 61.5 82 41.5 83 24.5 84 12.5 85 8.5 86 5.0 87 4.5 88 3.0 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35022707812585807 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010423424944221965 16-17 0.0018050321244872183 18-19 2.5422987668834062E-5 20-21 0.0 22-23 0.0 24-25 3.5592182736367685E-4 26-27 7.881126177338559E-4 28-29 0.0 30-31 0.003127027483266589 32-33 0.0 34-35 0.01108442262361165 36-37 0.027558518633016123 38-39 0.021329886654151777 40-41 0.013219953587793713 42-43 0.010220041042871292 44-45 0.0031778734586042573 46-47 0.0010677654820910305 48-49 6.609976793896855E-4 50-51 2.5422987668834062E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.004169369977688786 60-61 0.009813273240169947 62-63 0.00419479296535762 64-65 0.0020338390135067248 66-67 0.004042255039344616 68-69 0.003635487236643271 70-71 2.5422987668834062E-5 72-73 0.0 74-75 0.0 76-77 0.2176716204205572 78-79 3.0507585202600875E-4 80-81 0.0031016044955977555 82-83 3.813448150325109E-4 84-85 0.0 86-87 0.10710704704879791 88-89 0.39934429030204543 90-91 0.3863277206156024 92-93 0.0 94-95 0.0 96-97 2.5422987668834062E-5 98-99 0.001321995358779371 100-101 7.118436547273537E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1966724.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.42950596504581 #Duplication Level Percentage of deduplicated Percentage of total 1 74.50174919388887 35.335811577978994 2 13.306877754878595 12.622772757022997 3 4.6567458426578785 6.626014641661325 4 2.202394908962183 4.178340098880335 5 1.2984505366393295 3.0792433736426013 6 0.7978411806505816 2.270472782209558 7 0.5503663692715253 1.8272523496027093 8 0.38632357049908445 1.46585088731393 9 0.30052467195414684 1.2828363048983382 >10 1.7878697450760133 15.938763713465992 >50 0.13576269765447466 4.425140778723731 >100 0.06834585384239068 5.752254277071418 >500 0.003534495917887311 1.0849316859794065 >1k 0.0027847543595475783 2.527494081763328 >5k 3.213178107170283E-4 0.9194766828780849 >10k+ 1.0710593690567609E-4 0.6633440069072947 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13058 0.6639467459592704 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6486 0.32978699604011547 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6099 0.3101096035844379 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5515 0.28041555398723966 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4497 0.22865435109349352 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4202 0.21365478836888144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3533 0.17963883086798146 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3428 0.17430000345752633 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2859 0.14536864349039316 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2751 0.139877278153925 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2647 0.13458929671880754 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2498 0.12701324639349498 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2298 0.11684405132596135 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7796091368183842E-4 0.0 12-13 0.0 0.0 0.0 2.033839013506725E-4 0.0 14-15 0.0 0.0 0.0 2.033839013506725E-4 0.0 16-17 0.0 0.0 0.0 2.2880688901950655E-4 0.0 18-19 0.0 0.0 0.0 2.542298766883406E-4 0.0 20-21 0.0 0.0 0.0 3.5592182736367685E-4 0.0 22-23 0.0 0.0 0.0 5.338827410455153E-4 0.0 24-25 0.0 0.0 0.0 9.152275560780262E-4 0.0 26-27 0.0 0.0 0.0 0.0014999562724612096 0.0 28-29 0.0 0.0 0.0 0.0032795654092795936 0.0 30-31 0.0 0.0 0.0 0.009152275560780262 0.0 32-33 0.0 0.0 0.0 0.021889192382866126 0.0 34-35 0.0 0.0 0.0 0.03935478491135513 0.0 36-37 0.0 0.0 0.0 0.06782853110044929 0.0 38-39 0.0 0.0 0.0 0.10820023551855776 0.0 40-41 0.0 0.0 0.0 0.15253792601300437 0.0 42-43 0.0 0.0 0.0 0.2001297589290617 0.0 44-45 0.0 0.0 0.0 0.25961955007413345 0.0 46-47 0.0 0.0 0.0 0.3341089039438172 0.0 48-49 0.0 0.0 0.0 0.4137082783349367 0.0 50-51 0.0 0.0 0.0 0.5027904271265312 0.0 52-53 0.0 0.0 0.0 0.5924827276221778 0.0 54-55 0.0 0.0 0.0 0.6960559793850077 0.0 56-57 0.0 0.0 0.0 0.824721719976977 0.0 58-59 0.0 0.0 0.0 0.9629973499077654 0.0 60-61 0.0 0.0 0.0 1.114594625377023 0.0 62-63 0.0 0.0 0.0 1.2718358041087616 0.0 64-65 0.0 0.0 0.0 1.4327124700771434 0.0 66-67 0.0 0.0 0.0 1.6299185854242892 0.0 68-69 0.0 0.0 0.0 1.8535137619716848 0.0 70-71 0.0 0.0 0.0 2.1002184343100505 0.0 72-73 0.0 0.0 0.0 2.352465317960222 0.0 74-75 0.0 0.0 0.0 2.619940571224025 0.0 76-77 0.0 0.0 0.0 2.9104490513157923 0.0 78-79 0.0 0.0 0.0 3.249947628645402 0.0 80-81 0.0 0.0 0.0 3.629029797775387 5.0845975337668125E-5 82-83 0.0 0.0 0.0 3.9964936615407147 5.0845975337668125E-5 84-85 0.0 0.0 0.0 4.37710120993083 5.0845975337668125E-5 86-87 0.0 0.0 0.0 4.778225109369693 5.0845975337668125E-5 88-89 0.0 0.0 0.0 5.226025614168536 5.0845975337668125E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8370 0.0 27.592674 1 TACATGG 8310 0.0 26.778032 2 ACATGGG 8530 0.0 25.475533 3 CATGGGG 4755 0.0 21.553114 4 TACCTGG 1570 0.0 21.15462 2 GAGTACT 6525 0.0 20.433544 12-13 GTATAGG 360 3.6379788E-12 19.841124 1 CATGGGA 4265 0.0 19.80194 4 GTACTTT 6845 0.0 19.30499 14-15 TACGCTA 175 1.0027218E-4 18.978716 9 AGTACTT 6795 0.0 18.50437 12-13 AGAGTAC 11250 0.0 18.030237 10-11 GTACCTG 1945 0.0 17.872313 1 ATGGGAG 2020 0.0 17.616383 5 CCGACTA 165 0.0014753477 17.253378 9 GTAAGGT 660 0.0 17.253378 4 ACTTTTT 7785 0.0 17.217583 16-17 ATTCGCG 360 0.0 17.153841 80-81 ATGGGGA 2835 0.0 17.0708 5 CGTACAC 195 2.2959174E-4 17.03218 3 >>END_MODULE