FastQCFastQC Report
Fri 27 May 2016
SRR939106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935322
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT119190.6158665069688661No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT64490.3332262021513733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60670.31348788470342404No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48720.2517410539434781No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48230.24920917552737992No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44040.22755903152033613No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31340.16193687665411752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31270.16157517973753205No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26310.1359463696480482No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25080.12959083811376093No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24010.12406204238881177No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23110.11941165346128448No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20570.10628722248804076No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19910.10287693727452073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81400.027.9420191
TACATGG82900.026.4534172
ACATGGG83200.025.220113
TACCTGG16200.022.5128782
GAGTACT55150.021.33018912-13
GTATACG1150.003731486420.6451871
GTACCTG18450.020.3318731
CATGGGA41050.020.1925624
CATGGGG46350.019.9274354
GTACTTT60100.019.57413514-15
AGTACTT57750.019.09931212-13
AGAGTAC98250.018.64579610-11
GTGTAGG9200.018.580671
GTCGGGA4800.017.7617862
ACTTTTT68200.017.52836216-17
TGCACCG7650.017.336125
ATGGGAT18750.017.1776185
GTACTAG3059.320138E-817.1253531
TAAGGTG6100.017.0823735
TACTTTT64350.016.84680714-15
Produced by FastQC (version 0.11.5)