FastQCFastQC Report
Fri 27 May 2016
SRR939106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935322
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129720.6702760574209357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63090.3259922638196641No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60900.31467631742934765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51890.268120757165991No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43060.22249527468813976No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40120.20730400419155057No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34280.1771281471507067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.17661143726987033No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27610.142663598098921No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26950.13925331288540096No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26160.13517130482679368No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25970.13418955605320457No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22240.11491627749800808No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20010.1033936471553571No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19370.10008670391800434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG2150.033.1085438
GTACATG84150.028.9392171
TACATGG83950.027.9815482
ACATGGG86600.026.7965093
ATACCGT2751.8189894E-1224.15926
CCGTCGT2402.3283064E-1023.7284019
GTAAGCG4450.023.46118494-95
CATGGGG44700.023.3562134
TACCGTC2354.7039066E-922.2132477
GAGTACT63900.021.24099512-13
TACCTGG15950.020.8268992
GCACCGT6450.020.600876
GTACTTT68350.019.9275114-15
CATGGGA45900.019.6439194
CTAGATC2953.0540832E-919.3039634
ACCGTGC2953.0540832E-919.3039638
TTACGGT1507.772419E-418.982234
ATGGGGA25700.018.8345095
AGTACTT66350.018.27509512-13
AGAGTAC109100.017.74782410-11
Produced by FastQC (version 0.11.5)