##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939106_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1935322 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51220107041619 34.0 31.0 34.0 31.0 34.0 2 32.726243488163725 34.0 31.0 34.0 31.0 34.0 3 32.83631871078818 34.0 31.0 34.0 31.0 34.0 4 36.27278561397018 37.0 37.0 37.0 35.0 37.0 5 36.23302427192994 37.0 37.0 37.0 35.0 37.0 6 36.280504226170116 37.0 37.0 37.0 35.0 37.0 7 36.23434291554584 37.0 37.0 37.0 35.0 37.0 8 36.20897659407582 37.0 37.0 37.0 35.0 37.0 9 37.95734508262708 39.0 38.0 39.0 35.0 39.0 10-11 37.86914477280783 39.0 38.0 39.0 35.0 39.0 12-13 37.783129370719706 39.0 38.0 39.0 35.0 39.0 14-15 39.19383931976178 40.5 38.5 41.0 36.0 41.0 16-17 39.30503761131223 41.0 39.0 41.0 36.0 41.0 18-19 39.321266951959416 41.0 39.0 41.0 36.0 41.0 20-21 39.27833869505953 41.0 39.0 41.0 36.0 41.0 22-23 39.20112105375746 41.0 39.0 41.0 36.0 41.0 24-25 39.11166358879814 41.0 39.0 41.0 35.0 41.0 26-27 38.95952275641986 40.0 38.5 41.0 35.0 41.0 28-29 38.80805287182184 40.0 38.0 41.0 35.0 41.0 30-31 38.626670910577154 40.0 38.0 41.0 34.5 41.0 32-33 38.538656099605134 40.0 38.0 41.0 34.0 41.0 34-35 38.42029750088099 40.0 38.0 41.0 34.0 41.0 36-37 38.26844111729211 40.0 38.0 41.0 34.0 41.0 38-39 38.1295763702371 40.0 38.0 41.0 33.5 41.0 40-41 37.947188116499476 40.0 37.5 41.0 33.0 41.0 42-43 37.73488236066143 40.0 37.0 41.0 33.0 41.0 44-45 37.46282530762323 40.0 37.0 41.0 33.0 41.0 46-47 37.144162056753345 40.0 36.5 41.0 31.5 41.0 48-49 36.77653072718648 40.0 36.0 41.0 31.0 41.0 50-51 36.48568326097673 39.0 35.5 41.0 30.5 41.0 52-53 36.1988930007513 39.0 35.0 40.5 30.0 41.0 54-55 35.92021715249452 39.0 35.0 40.0 29.0 41.0 56-57 35.615427561925095 38.0 35.0 40.0 28.5 41.0 58-59 35.23322992246251 38.0 34.5 40.0 28.0 41.0 60-61 34.78396644072666 37.5 34.0 40.0 27.0 41.0 62-63 34.36145871333039 37.0 34.0 39.5 26.0 41.0 64-65 33.86763649666567 36.0 33.0 39.0 26.0 40.5 66-67 33.39207119022054 35.5 33.0 39.0 25.5 40.0 68-69 32.87186731716996 35.0 32.5 38.0 24.5 40.0 70-71 32.32801724984266 35.0 32.0 37.0 22.5 39.5 72-73 31.73358593557041 35.0 31.0 37.0 21.0 39.0 74-75 31.188978629912746 34.5 31.0 36.0 19.5 39.0 76-77 29.243395155948207 32.5 28.5 34.5 16.5 36.0 78-79 30.240273711558075 34.0 30.0 35.0 18.0 37.0 80-81 30.258164791182036 34.0 30.0 35.0 16.0 37.0 82-83 29.887353887363446 34.0 30.0 35.0 10.0 36.0 84-85 29.416357071329735 34.0 29.5 35.0 6.0 36.0 86-87 28.726583999975198 34.0 29.0 35.0 2.0 35.0 88-89 28.046487354559087 33.0 27.5 35.0 2.0 35.0 90-91 27.46751005775783 33.0 26.5 35.0 2.0 35.0 92-93 26.99013497495507 33.0 25.5 34.0 2.0 35.0 94-95 26.42304019692847 32.0 25.0 34.0 2.0 35.0 96-97 25.56278309242596 32.0 23.5 34.0 2.0 35.0 98-99 24.290160242068247 32.0 10.0 34.0 2.0 35.0 100-101 18.388824185329366 24.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0852763885631802 1101 2 0.16616619978121605 1101 3 0.18371353466988438 1101 4 0.13301238491511214 1101 5 0.1756367519849391 1101 6 0.12637845333576792 1101 7 0.16454676990478134 1101 8 0.17154271933637233 1101 9 0.13434554722274328 1101 10-11 0.1909342195207131 1101 12-13 0.20804287826816648 1101 14-15 0.3626826627509061 1101 16-17 0.23489123819213376 1101 18-19 0.36816753809958414 1101 20-21 0.27236472982213655 1101 22-23 0.21492126441321346 1101 24-25 0.2999644313822287 1101 26-27 0.3288527923465949 1101 28-29 0.3271514430391207 1101 30-31 0.25971209839665477 1101 32-33 0.3256531837984298 1101 34-35 0.3824650286001159 1101 36-37 0.3877468667814412 1101 38-39 0.25307281313250485 1101 40-41 0.3349010104602357 1101 42-43 0.37781104759893225 1101 44-45 0.5009750449761157 1101 46-47 0.4625736690074831 1101 48-49 0.5684560760204604 1101 50-51 0.5225341252627658 1101 52-53 0.5235709847574057 1101 54-55 0.4549406609810873 1101 56-57 0.561232362290994 1101 58-59 0.4591627323106593 1101 60-61 0.6559228089206286 1101 62-63 0.47607186175112304 1101 64-65 0.3534363535156899 1101 66-67 0.42864889963492203 1101 68-69 0.5994693751894573 1101 70-71 0.4793201044562849 1101 72-73 0.40279252197960247 1101 74-75 0.38728122985512314 1101 76-77 0.3748445549177859 1101 78-79 0.46520842256429873 1101 80-81 0.4744714219854096 1101 82-83 0.4839040507885777 1101 84-85 0.5660406022828788 1101 86-87 0.772394747658776 1101 88-89 0.737810707566684 1101 90-91 0.7371949619337634 1101 92-93 0.34282378946092606 1101 94-95 0.24331148525151391 1101 96-97 0.13205124087403064 1101 98-99 -0.12593225713862566 1101 100-101 -0.8593671491465642 1102 1 0.13850098548856948 1102 2 0.16124962685282895 1102 3 0.1391013227348381 1102 4 0.18954089577432853 1102 5 0.20229880384384558 1102 6 0.22380065056110965 1102 7 0.1938197934691459 1102 8 0.20873158034412853 1102 9 0.32118541339331586 1102 10-11 0.30328117374759245 1102 12-13 0.272250176875076 1102 14-15 0.3844750108227828 1102 16-17 0.4487527867485639 1102 18-19 0.3661164723674233 1102 20-21 0.3511748304826341 1102 22-23 0.31564304919012187 1102 24-25 0.3964310389216692 1102 26-27 0.4783922128997915 1102 28-29 0.4816281671024569 1102 30-31 0.4514380725537279 1102 32-33 0.37346259223776457 1102 34-35 0.4037407345214916 1102 36-37 0.4865057358651157 1102 38-39 0.4482137450352468 1102 40-41 0.3319500572140086 1102 42-43 0.42078554357539844 1102 44-45 0.4387259751483015 1102 46-47 0.39098051478637785 1102 48-49 0.24034162157726513 1102 50-51 0.18834903541823422 1102 52-53 0.16020931362015034 1102 54-55 0.17203744215266425 1102 56-57 0.11058574840619428 1102 58-59 -0.06076674765818524 1102 60-61 -0.09755938801629327 1102 62-63 -0.11335211650159494 1102 64-65 0.2117071345719097 1102 66-67 0.2071974344334535 1102 68-69 0.16115493372540612 1102 70-71 0.18313489203091393 1102 72-73 0.19903451817238604 1102 74-75 0.2025722872229494 1102 76-77 0.25752712824996493 1102 78-79 0.2877283239227886 1102 80-81 0.3337702596307004 1102 82-83 0.35177345172884245 1102 84-85 0.259210686791544 1102 86-87 0.49306007314693545 1102 88-89 0.18488481128449052 1102 90-91 0.36949933734837614 1102 92-93 0.13208030066625653 1102 94-95 -0.05813424749343099 1102 96-97 0.0033911214658779443 1102 98-99 0.21155296105525778 1102 100-101 -0.4718159536229294 1103 1 0.03627793221839681 1103 2 0.032033097309074776 1103 3 0.07654418818817277 1103 4 0.023522446509808503 1103 5 0.11942670212357598 1103 6 0.08163535512356646 1103 7 0.05708955163419205 1103 8 0.10986803841775838 1103 9 0.1388306390228351 1103 10-11 0.07238125269714146 1103 12-13 0.08162699479246527 1103 14-15 -0.005613703546039517 1103 16-17 0.05745333939545105 1103 18-19 0.19600822837173837 1103 20-21 0.191840084367783 1103 22-23 0.18293507944026288 1103 24-25 0.11051678229406292 1103 26-27 0.1476003280830369 1103 28-29 0.16746399357963782 1103 30-31 0.19519534422330054 1103 32-33 0.0390239651289761 1103 34-35 0.05453854055490126 1103 36-37 0.023626050832291412 1103 38-39 0.05100866317701502 1103 40-41 0.1465172889932731 1103 42-43 0.15272842980493095 1103 44-45 0.07106724498957817 1103 46-47 0.07522332622160377 1103 48-49 -0.0020298776332410284 1103 50-51 0.027954183760286355 1103 52-53 0.10445326774630104 1103 54-55 0.047659689306321695 1103 56-57 -0.06894294352432695 1103 58-59 -0.053459758544910585 1103 60-61 0.13004958314633797 1103 62-63 0.12169992787636374 1103 64-65 -0.020129793043821564 1103 66-67 -0.07137347473300082 1103 68-69 -0.04374824918824771 1103 70-71 -0.11297891998604115 1103 72-73 -0.20189573612828937 1103 74-75 -0.1341054947100666 1103 76-77 -0.041543136854333085 1103 78-79 -0.007045423272426632 1103 80-81 0.03760554592671994 1103 82-83 -0.14703814970055973 1103 84-85 -0.2859733903297048 1103 86-87 -0.272693479583058 1103 88-89 -0.21376006114964952 1103 90-91 -0.09301001194107883 1103 92-93 -0.31400026943263626 1103 94-95 -0.2817463974144836 1103 96-97 -0.7020169230782862 1103 98-99 -0.9578852505295536 1103 100-101 -1.050359334325993 1104 1 0.07712425181883731 1104 2 0.10720910792377936 1104 3 0.1915388003315499 1104 4 0.08290676557331977 1104 5 0.020458563404879726 1104 6 0.07118739333002111 1104 7 0.0435392353860351 1104 8 0.09036295321753585 1104 9 0.13310189394037053 1104 10-11 0.1339479989605934 1104 12-13 0.132567924414154 1104 14-15 0.09298929377974474 1104 16-17 0.11156281377037658 1104 18-19 0.09973553377310651 1104 20-21 0.16958481752318733 1104 22-23 0.2299694340578995 1104 24-25 0.15717858490010883 1104 26-27 0.21333841054503466 1104 28-29 0.18283277459686786 1104 30-31 0.12845143869925835 1104 32-33 -0.01904850600582364 1104 34-35 0.06789685441010107 1104 36-37 0.13828162717126702 1104 38-39 0.08916678085292773 1104 40-41 0.13677434651281573 1104 42-43 0.0555955981704912 1104 44-45 0.18314342076001822 1104 46-47 0.17456149060461712 1104 48-49 0.2968139311148619 1104 50-51 0.2188631736154889 1104 52-53 0.221881426008828 1104 54-55 0.21066449980838797 1104 56-57 0.25437055966711597 1104 58-59 0.20236709302342604 1104 60-61 0.21973474301191231 1104 62-63 0.158396132595378 1104 64-65 0.2546406209369252 1104 66-67 0.3146896105163961 1104 68-69 0.33649615301567337 1104 70-71 0.10500291254543015 1104 72-73 -0.15101459672545658 1104 74-75 -0.12208597648786323 1104 76-77 -0.27196352440947535 1104 78-79 -0.03205929291896936 1104 80-81 0.2656435880008452 1104 82-83 0.22223971582568325 1104 84-85 0.25235045572749115 1104 86-87 0.24091797426916983 1104 88-89 -0.06472864585285265 1104 90-91 -0.08113415866728602 1104 92-93 -0.4726171515630426 1104 94-95 -0.32058427093071984 1104 96-97 -0.3785077121845468 1104 98-99 -0.8276052268290464 1104 100-101 -0.9975869713525434 1105 1 -0.025527328539745042 1105 2 0.013469704072910815 1105 3 0.07048806662157858 1105 4 0.022729441462878697 1105 5 -0.04092835188330923 1105 6 0.0559113326717906 1105 7 0.08405467120402221 1105 8 0.0388474104599581 1105 9 0.15720536191326318 1105 10-11 0.11197647619289341 1105 12-13 0.09820738408227925 1105 14-15 0.14404708289485058 1105 16-17 0.11136539936117629 1105 18-19 0.16764478639573355 1105 20-21 0.15409471465251556 1105 22-23 0.15207322937029488 1105 24-25 0.14393747392810496 1105 26-27 0.16313320389078 1105 28-29 0.17692256864685163 1105 30-31 0.15115654102219622 1105 32-33 0.19744907872424733 1105 34-35 0.2251451746759372 1105 36-37 0.2000112056205836 1105 38-39 0.2120972714187701 1105 40-41 0.3104330044469492 1105 42-43 0.38434731963717894 1105 44-45 0.3784058335781637 1105 46-47 0.45537884168470555 1105 48-49 0.5100327412683825 1105 50-51 0.5129548912740844 1105 52-53 0.6977817074425374 1105 54-55 0.5229222555374804 1105 56-57 0.45411856693900887 1105 58-59 0.35362353548210024 1105 60-61 0.3340204060634946 1105 62-63 0.22311023871075975 1105 64-65 0.35815179351656923 1105 66-67 0.39809190032791975 1105 68-69 0.11696252703299592 1105 70-71 0.11896179035780818 1105 72-73 0.03967158380945435 1105 74-75 -0.007015872365212061 1105 76-77 -0.1855713273484909 1105 78-79 -0.18450462331016126 1105 80-81 -0.09391635555591549 1105 82-83 -0.05446046075506672 1105 84-85 -0.11163069029982964 1105 86-87 -0.12880286121599838 1105 88-89 -0.021333013683335622 1105 90-91 -0.06112324952421133 1105 92-93 -0.09849402365806625 1105 94-95 -0.1588340544432434 1105 96-97 -0.6136214267966658 1105 98-99 -0.6067707197684236 1105 100-101 -0.9633056576126329 1106 1 -0.004181300015360989 1106 2 0.02487305247929328 1106 3 0.03173496371193352 1106 4 -0.07316321403082782 1106 5 -0.007882364150688659 1106 6 0.002672491953660483 1106 7 0.05674937722976381 1106 8 0.03207635171484924 1106 9 0.06855137800113908 1106 10-11 0.0355211556044992 1106 12-13 0.05772436240793155 1106 14-15 0.10250602499543504 1106 16-17 0.11316356348333301 1106 18-19 -0.019631667308104284 1106 20-21 0.09175685567260672 1106 22-23 0.08927179942019592 1106 24-25 0.06439681026609634 1106 26-27 0.00919126498887124 1106 28-29 0.1486797171537262 1106 30-31 0.07381636263287561 1106 32-33 0.025441241234631207 1106 34-35 -0.021771347727202794 1106 36-37 -0.0780486736955055 1106 38-39 0.009644387142998312 1106 40-41 -0.008921534382331231 1106 42-43 -0.013122814080190892 1106 44-45 0.05775354476256922 1106 46-47 -0.01110709137701349 1106 48-49 -0.08654811968644083 1106 50-51 0.028532865714566924 1106 52-53 0.025729875793111034 1106 54-55 -0.22892394461076293 1106 56-57 -0.30021415824889885 1106 58-59 -0.30878875601978706 1106 60-61 -0.4787620582064065 1106 62-63 -0.4666186950822251 1106 64-65 -0.3272375750071177 1106 66-67 -0.29105547109097785 1106 68-69 -0.4531386688619534 1106 70-71 -0.4895784865286821 1106 72-73 -0.4850128707997676 1106 74-75 -0.5501940415614648 1106 76-77 -0.4191047641535981 1106 78-79 -0.4229968082062676 1106 80-81 -0.33749134274630777 1106 82-83 -0.3504931202782018 1106 84-85 -0.5855974960066987 1106 86-87 -0.4852388803383789 1106 88-89 -0.42188351586967343 1106 90-91 -0.4326784912651256 1106 92-93 -0.4621010908301564 1106 94-95 -0.7588803493576997 1106 96-97 -1.0377128463498515 1106 98-99 -0.8535636543374494 1106 100-101 -1.4578217778189781 1107 1 -0.017180892092319766 1107 2 -0.040662845051407714 1107 3 -0.012001599224291226 1107 4 -8.77796400672537E-4 1107 5 -0.009864700416258643 1107 6 0.08208171271154896 1107 7 0.06207419731630637 1107 8 0.05631534491472223 1107 9 0.050738233423636814 1107 10-11 0.10569316214338187 1107 12-13 0.13955945239647605 1107 14-15 0.10467716962264006 1107 16-17 0.04054523094907836 1107 18-19 0.09151569352679445 1107 20-21 0.13714132465439377 1107 22-23 0.09395442862663117 1107 24-25 0.11976130132300256 1107 26-27 0.08311458757962953 1107 28-29 0.08186534759484942 1107 30-31 0.09029875678351829 1107 32-33 0.049624319277647544 1107 34-35 0.05173874821654323 1107 36-37 0.09357312648766936 1107 38-39 0.02789560954833803 1107 40-41 -0.01711363522235132 1107 42-43 0.06171682003768808 1107 44-45 -0.015223766109336623 1107 46-47 -0.06529039368678013 1107 48-49 -0.13147996380771332 1107 50-51 -0.1989199912923425 1107 52-53 -0.08477984010939821 1107 54-55 -0.1328453397196867 1107 56-57 -0.0010004859777907882 1107 58-59 -0.07827890070821297 1107 60-61 -0.031051821762567045 1107 62-63 -0.22057109355454685 1107 64-65 -0.23212649932072793 1107 66-67 -0.3132239366396732 1107 68-69 -0.41684412539329685 1107 70-71 -0.48555532280364844 1107 72-73 -0.5024385491006278 1107 74-75 -0.6632256977229503 1107 76-77 -0.40923035959698595 1107 78-79 -0.20589916549230125 1107 80-81 -0.21897136391223881 1107 82-83 -0.32160483482178037 1107 84-85 -0.4212242094501555 1107 86-87 -0.5281326245688156 1107 88-89 -0.4781428009678734 1107 90-91 -0.05071523585741744 1107 92-93 -0.0449915983458169 1107 94-95 -0.03627574558553448 1107 96-97 -0.1240708512485611 1107 98-99 -0.19125922858459532 1107 100-101 -0.948740186543322 1108 1 -0.12483638335727676 1108 2 -0.07356171293731961 1108 3 -0.08840069502142 1108 4 -0.03877326887457855 1108 5 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-0.5586338820857364 2316 50-51 -0.5874923107356196 2316 52-53 -0.4980667852809475 2316 54-55 -0.6378755615103344 2316 56-57 -0.33093210873666123 2316 58-59 -0.3238333685373078 2316 60-61 -0.2633732616426627 2316 62-63 -0.1892793143037892 2316 64-65 -0.11412276469549454 2316 66-67 -0.16409949780098287 2316 68-69 -0.06125234649437061 2316 70-71 -0.018490565844537343 2316 72-73 0.07433786066322767 2316 74-75 -0.05321411387594921 2316 76-77 -0.07941769775736063 2316 78-79 -0.11825267424571351 2316 80-81 0.014316276114218596 2316 82-83 -0.052576365120973634 2316 84-85 -0.09754869965534141 2316 86-87 -0.2836397894904259 2316 88-89 -0.7068677070305291 2316 90-91 -0.8426114685124304 2316 92-93 -0.9678796352311814 2316 94-95 -0.7137489452956309 2316 96-97 -0.5072945470322878 2316 98-99 -0.37354891623412456 2316 100-101 0.5514097789778027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 21.0 5 38.0 6 76.0 7 193.0 8 470.0 9 927.0 10 1504.0 11 2376.0 12 3627.0 13 5143.0 14 6268.0 15 8506.0 16 10834.0 17 15832.0 18 16307.0 19 11834.0 20 11562.0 21 12543.0 22 13406.0 23 14292.0 24 16399.0 25 19064.0 26 21794.0 27 26507.0 28 31725.0 29 38357.0 30 47082.0 31 58361.0 32 73283.0 33 96440.0 34 136243.0 35 207518.0 36 330885.0 37 455472.0 38 232620.0 39 7807.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69113441867465 18.183186378049605 13.657084732392613 23.468594470883133 2 16.38006491942943 20.22991522857695 38.38219169729895 25.00782815469467 3 20.87735270926492 23.12974275081873 28.554524776755496 27.43837976316086 4 13.737972285748832 15.559994667554031 34.90984962709048 35.79218341960666 5 14.752790491711457 36.77202036663667 32.80606534726521 15.669123794386671 6 34.05319631565187 34.334338161814934 16.154211030515853 15.458254492017348 7 30.709411663795482 31.384699807060528 19.457795653643167 18.44809287550082 8 27.24575031958506 33.95006102343693 18.5409973120752 20.26319134490281 9 26.665795149334322 17.06863250663197 19.809675082492735 36.455897261540976 10-11 26.571805622010185 25.29338787033889 25.71794254392809 22.416863963722832 12-13 27.573525232493612 22.54598976294384 26.56033466265562 23.320150341906928 14-15 23.61529434925694 24.290046571518776 25.31125617022244 26.783402909001847 16-17 23.452021922471463 27.568078843595572 25.683669164245178 23.296230069687788 18-19 23.300211360231362 26.43865631627613 27.486854251347903 22.7742780721446 20-21 24.126966985338875 25.038314037664016 27.381774195715238 23.452944781281875 22-23 23.60418576340268 24.948768215315074 27.726445521727133 23.720600499555115 24-25 24.103423696488516 25.17967954145063 27.488082723249196 23.22881403881166 26-27 23.515823354681924 25.09514888594871 28.25555682153761 23.133470937831753 28-29 23.026076811030315 26.172944953317824 27.792469569647622 23.008508666004243 30-31 23.26594747540719 25.258897485793064 27.560349130532284 23.91480590826746 32-33 23.3355543252116 25.48411207130185 27.728028650536874 23.452304952949678 34-35 24.158710554262193 25.240905485260413 27.444621581886157 23.155762378591234 36-37 23.969165876965864 24.963710803856408 27.751486002849386 23.315637316328342 38-39 23.37893797054412 25.56258567827477 27.84155995508876 23.216916396092362 40-41 23.46259055838925 25.61599341445208 27.551660817410983 23.369755209747687 42-43 23.304726408330158 25.69494415771489 26.88519549644405 24.115133937510898 44-45 23.6072313287303 25.268105450159823 27.086530601277563 24.03813261983231 46-47 24.11410314311492 26.003661610550566 26.70640042909983 23.175834817234684 48-49 24.049086982436712 25.734443228126892 26.691188799842504 23.525280989593895 50-51 23.23355682052758 26.11165093317164 27.17075856381554 23.484033682485247 52-53 23.966451060857054 26.32802190023159 26.212046367477864 23.49348067143349 54-55 23.363967339801853 25.669320144141388 26.565682609922277 24.401029906134482 56-57 23.78159069776815 25.823552223003894 26.496929061620545 23.89792801760742 58-59 24.46679825762547 25.401851918833867 26.337680657679215 23.79366916586145 60-61 23.76185746957285 25.71671873498278 27.282488614038915 23.238935181405463 62-63 23.3053815295907 25.978255000334578 27.067494343887017 23.648869126187705 64-65 23.0719724772894 26.36623383401166 26.61720598292387 23.94458770577507 66-67 23.41767524295611 25.923775789260194 26.269850189443115 24.388698778340572 68-69 23.15631108747921 25.81469349748894 26.79080133337674 24.238194081655116 70-71 23.685075336378482 25.721586094289318 26.54460244300684 24.048736126325363 72-73 23.531536354157087 25.487541608063154 26.9570128381737 24.02390919960606 74-75 22.968270913057363 26.760611412467796 26.156758410228377 24.114359264246467 76-77 23.369704361264855 25.790146616827187 26.350349409100097 24.489799612807854 78-79 23.454615739219054 25.888920241365838 26.56732238132743 24.08914163808768 80-81 23.563230566202694 26.03487082716293 26.418607006408262 23.983291600226114 82-83 23.48872694052979 25.801339518695084 26.614537529155356 24.095396011619773 84-85 23.16857349836358 26.02003697575907 26.45515836641138 24.35623115946597 86-87 23.22918883884962 26.06737481440024 26.367981122037676 24.33545522471246 88-89 22.93099337586842 26.126028429310555 25.966113233138145 24.976864961682878 90-91 23.180454666451496 25.729190611523318 26.188412963872953 24.901941758152233 92-93 23.4118146747673 25.805860730152396 26.30660427567092 24.47572031940938 94-95 23.1080409358236 25.42582061279725 26.59572928949291 24.87040916188624 96-97 22.716122360031655 25.378625721876187 26.943223645270308 24.962028272821854 98-99 22.799503027537845 25.76893674090337 26.118259087586875 25.313301143971913 100-101 23.15352960811527 24.111100317571204 26.932270422267397 25.80309965204613 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 897.0 1 609.0 2 470.0 3 619.5 4 508.0 5 458.0 6 748.5 7 1448.5 8 2227.0 9 2529.5 10 2211.5 11 1833.0 12 1791.5 13 1903.5 14 2045.0 15 2180.5 16 2250.5 17 2343.0 18 2451.0 19 2507.5 20 2702.0 21 3043.5 22 3537.0 23 4290.0 24 5136.5 25 5949.5 26 7188.0 27 8689.5 28 10030.0 29 11891.5 30 14292.5 31 16891.5 32 20359.5 33 22868.5 34 24349.0 35 26530.0 36 30383.0 37 34762.5 38 37569.0 39 39057.0 40 40595.0 41 44052.0 42 47701.5 43 51504.5 44 55794.0 45 60215.5 46 63390.0 47 73237.5 48 93951.0 49 95262.0 50 80894.5 51 75876.5 52 75125.0 53 77174.5 54 79154.0 55 75881.5 56 69804.0 57 64612.0 58 60554.0 59 55105.5 60 47369.5 61 39747.5 62 33575.0 63 27124.0 64 20632.0 65 15723.0 66 11930.0 67 9808.5 68 7574.0 69 5134.5 70 3778.5 71 2910.0 72 2324.5 73 1976.5 74 1588.5 75 1199.0 76 787.5 77 452.5 78 287.0 79 169.0 80 94.5 81 54.5 82 38.5 83 22.0 84 11.5 85 8.5 86 6.0 87 2.0 88 1.5 89 2.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4356897715212249 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.817487735890977E-4 16-17 0.0024543719339727445 18-19 2.5835494041818366E-5 20-21 0.0 22-23 0.0 24-25 7.750648212545509E-5 26-27 0.003384449719478206 28-29 5.167098808363673E-5 30-31 0.0 32-33 2.5835494041818366E-5 34-35 0.011729314294985537 36-37 0.026739736333282006 38-39 0.02038420479899469 40-41 0.009068258408678247 42-43 0.009533297301430977 44-45 0.003926995094356391 46-47 0.005580466713032767 48-49 0.0032811077433109325 50-51 6.975583391290958E-4 52-53 0.0 54-55 0.0 56-57 7.750648212545509E-5 58-59 0.0016534716186763754 60-61 0.0022735234756800157 62-63 0.0013692811842163732 64-65 0.0024543719339727445 66-67 0.0022993589697218346 68-69 0.004392033987109122 70-71 1.0334197616727346E-4 72-73 0.0 74-75 0.0 76-77 0.09894994218016433 78-79 0.01725811001993467 80-81 4.1336790466909386E-4 82-83 0.0 84-85 0.0 86-87 0.10657141292250076 88-89 0.41597728956731744 90-91 0.42117022386972297 92-93 0.0 94-95 0.0 96-97 2.5835494041818366E-5 98-99 1.8084845829272853E-4 100-101 4.1336790466909386E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1935322.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.66275770467925 #Duplication Level Percentage of deduplicated Percentage of total 1 74.51008990609087 35.51356361747877 2 13.449461778877133 12.820768760499304 3 4.608237327151974 6.5892389750910985 4 2.1915975031366224 4.178303231127234 5 1.2446700446270669 2.9662203379666097 6 0.779726934849335 2.229836158292165 7 0.5593855010090631 1.8663298918673916 8 0.3779641277961041 1.4411850115364908 9 0.296736754833194 1.2728962842918603 >10 1.7714837744759249 15.826973437032937 >50 0.13734537063576516 4.462974303059891 >100 0.06712112920375284 5.787023224174334 >500 0.0031441328436082544 1.0367075989390673 >1k 0.0026020409740206245 2.4287854579670567 >5k 3.2525512175257806E-4 0.9088631866284674 >10k+ 1.0841837391752602E-4 0.6703305240473322 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12972 0.6702760574209357 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6309 0.3259922638196641 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6090 0.31467631742934765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5189 0.268120757165991 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4306 0.22249527468813976 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4012 0.20730400419155057 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3428 0.1771281471507067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3418 0.17661143726987033 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2761 0.142663598098921 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2695 0.13925331288540096 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2616 0.13517130482679368 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2597 0.13418955605320457 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2224 0.11491627749800808 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2001 0.1033936471553571 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1937 0.10008670391800434 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.1670988083636725E-5 2 0.0 0.0 0.0 0.0 5.1670988083636725E-5 3 0.0 0.0 0.0 0.0 5.1670988083636725E-5 4 0.0 0.0 0.0 5.1670988083636725E-5 5.1670988083636725E-5 5 0.0 0.0 0.0 5.1670988083636725E-5 5.1670988083636725E-5 6 0.0 0.0 0.0 5.1670988083636725E-5 5.1670988083636725E-5 7 0.0 0.0 0.0 5.1670988083636725E-5 5.1670988083636725E-5 8 0.0 0.0 0.0 5.1670988083636725E-5 5.1670988083636725E-5 9 0.0 0.0 0.0 5.1670988083636725E-5 5.1670988083636725E-5 10-11 0.0 0.0 0.0 5.1670988083636725E-5 5.1670988083636725E-5 12-13 0.0 0.0 0.0 1.0334197616727345E-4 5.1670988083636725E-5 14-15 0.0 0.0 0.0 1.291774702090918E-4 5.1670988083636725E-5 16-17 0.0 0.0 0.0 2.066839523345469E-4 5.1670988083636725E-5 18-19 0.0 0.0 0.0 2.8419043446000197E-4 5.1670988083636725E-5 20-21 0.0 0.0 0.0 3.6169691658545706E-4 5.1670988083636725E-5 22-23 0.0 0.0 0.0 6.200518570036407E-4 5.1670988083636725E-5 24-25 0.0 0.0 0.0 0.0011367617378400079 5.1670988083636725E-5 26-27 0.0 0.0 0.0 0.001782649088885467 5.1670988083636725E-5 28-29 0.0 0.0 0.0 0.003771982130105481 5.1670988083636725E-5 30-31 0.0 0.0 0.0 0.009610803783556433 5.1670988083636725E-5 32-33 0.0 0.0 0.0 0.02177932147725288 5.1670988083636725E-5 34-35 0.0 0.0 0.0 0.03994167378865119 5.1670988083636725E-5 36-37 0.0 0.0 0.0 0.06983334039503504 5.1670988083636725E-5 38-39 0.0 0.0 0.0 0.11197103117724079 5.1670988083636725E-5 40-41 0.0 0.0 0.0 0.15976669515460476 5.1670988083636725E-5 42-43 0.0 0.0 0.0 0.21024925051231785 5.1670988083636725E-5 44-45 0.0 0.0 0.0 0.2716343843556783 5.1670988083636725E-5 46-47 0.0 0.0 0.0 0.3471773689339552 5.1670988083636725E-5 48-49 0.0 0.0 0.0 0.43088436962944665 5.1670988083636725E-5 50-51 0.0 0.0 0.0 0.5221353345851492 5.1670988083636725E-5 52-53 0.0 0.0 0.0 0.6127920831778897 5.1670988083636725E-5 54-55 0.0 0.0 0.0 0.7142997392681941 5.1670988083636725E-5 56-57 0.0 0.0 0.0 0.841772066870526 5.1670988083636725E-5 58-59 0.0 0.0 0.0 0.9795010856074596 5.1670988083636725E-5 60-61 0.0 0.0 0.0 1.1388285773633535 5.1670988083636725E-5 62-63 0.0 0.0 0.0 1.294823290387853 5.1670988083636725E-5 64-65 0.0 0.0 0.0 1.461927265850334 5.1670988083636725E-5 66-67 0.0 0.0 0.0 1.656907739383937 5.1670988083636725E-5 68-69 0.0 0.0 0.0 1.884776796832775 5.1670988083636725E-5 70-71 0.0 0.0 0.0 2.132539184693813 5.1670988083636725E-5 72-73 0.0 0.0 0.0 2.3838668707326223 5.1670988083636725E-5 74-75 0.0 0.0 0.0 2.641782607752095 5.1670988083636725E-5 76-77 0.0 0.0 0.0 2.9231569733615386 5.1670988083636725E-5 78-79 0.0 0.0 0.0 3.261963642225945 5.1670988083636725E-5 80-81 0.0 0.0 0.0 3.636604141326353 5.1670988083636725E-5 82-83 0.0 0.0 0.0 4.003959031106968 5.1670988083636725E-5 84-85 0.0 0.0 0.0 4.384154161426367 5.1670988083636725E-5 86-87 0.0 0.0 0.0 4.7772412032726335 1.0334197616727345E-4 88-89 0.0 0.0 0.0 5.216961311864383 1.0334197616727345E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCG 215 0.0 33.108543 8 GTACATG 8415 0.0 28.939217 1 TACATGG 8395 0.0 27.981548 2 ACATGGG 8660 0.0 26.796509 3 ATACCGT 275 1.8189894E-12 24.1592 6 CCGTCGT 240 2.3283064E-10 23.728401 9 GTAAGCG 445 0.0 23.461184 94-95 CATGGGG 4470 0.0 23.356213 4 TACCGTC 235 4.7039066E-9 22.213247 7 GAGTACT 6390 0.0 21.240995 12-13 TACCTGG 1595 0.0 20.826899 2 GCACCGT 645 0.0 20.60087 6 GTACTTT 6835 0.0 19.92751 14-15 CATGGGA 4590 0.0 19.643919 4 CTAGATC 295 3.0540832E-9 19.303963 4 ACCGTGC 295 3.0540832E-9 19.303963 8 TTACGGT 150 7.772419E-4 18.98223 4 ATGGGGA 2570 0.0 18.834509 5 AGTACTT 6635 0.0 18.275095 12-13 AGAGTAC 10910 0.0 17.747824 10-11 >>END_MODULE