FastQCFastQC Report
Fri 27 May 2016
SRR939105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935280
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT146380.49869177727508107No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT77810.2650854432967213No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT66440.22634978605107517No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC60410.2058066010738328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41480.14131530893134556No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40600.13831729852007305No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35680.12155569485704941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33820.11521899103322342No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31310.1066678477010711No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31190.10625902809953394No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30960.10547545719658771No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG83800.026.3978121
TACATGG85800.025.1792852
ACATGGG87050.023.9469263
CATGGGG51000.020.4370574
GAGTACT57500.019.10834512-13
AGAGTAC100400.018.49078210-11
GTGTAGC16750.017.8546681
GTACTTT60550.017.83342414-15
AGTACTT63200.017.38496612-13
GAGTACA71800.016.9916151
AGTACAT63450.016.8749562
GTCCTAG12550.016.6431271
CCGTAGA2801.37240495E-515.2282622
GTCCTAT9050.015.2116181
GTCCTAA8750.015.1906381
AAGACCG4702.7102942E-1015.120265
TAGCCCT16800.014.9462584
CGATCGT3500.014.88845924-25
ATGGGTA11500.014.8310035
GTATAGA8800.014.5648861
Produced by FastQC (version 0.11.5)