FastQCFastQC Report
Fri 27 May 2016
SRR939105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2935280
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT162010.5519405303752963No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT71510.24362241421602027No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56260.19166825652067265No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54330.18509307459594998No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT46220.15746368319206344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45420.1547382191818157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36940.1258483006731896No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG35610.12131721675615273No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35040.11937532364885123No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34390.11716088414052493No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33120.11283421002425663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG89800.024.7579921
TACATGG92950.023.9416082
ACATGGG93600.023.2177163
GAGTACT62400.019.16352712-13
CATGGGG53300.018.9619184
CATGGGA42400.017.7934914
GTACTTT67850.017.23957414-15
GTGTAGC16350.017.1794451
AGAGTAC111000.016.67251410-11
GAGTACA77600.016.0122991
AGTACTT68250.015.8870412-13
GTATAGG9000.015.8691481
AGTACAT70000.015.5904872
ATGGGAG24500.015.299985
TACACTG22900.014.5041555
GGACAGT22450.014.3721736
CCTACAC11000.014.2347933
ATGGGGA33950.014.11599355
GAACCGC5057.7852746E-1014.0938536
GTAAGCG3900.013.99170194-95
Produced by FastQC (version 0.11.5)