##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939105_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2935280 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.549178613283914 34.0 31.0 34.0 31.0 34.0 2 32.760482815949416 34.0 31.0 34.0 31.0 34.0 3 32.85856851816522 34.0 31.0 34.0 31.0 34.0 4 36.2853996892971 37.0 37.0 37.0 35.0 37.0 5 36.24682142759805 37.0 37.0 37.0 35.0 37.0 6 36.282162860101934 37.0 37.0 37.0 35.0 37.0 7 36.24003842904254 37.0 37.0 37.0 35.0 37.0 8 36.22674770379657 37.0 37.0 37.0 35.0 37.0 9 37.986222438745195 39.0 38.0 39.0 35.0 39.0 10-11 37.926871201384536 39.0 38.0 39.0 35.0 39.0 12-13 37.869113168079366 39.0 38.0 39.0 35.0 39.0 14-15 39.27099016107492 41.0 39.0 41.0 36.0 41.0 16-17 39.22315860837807 40.5 39.0 41.0 36.0 41.0 18-19 39.23611290916028 40.5 39.0 41.0 36.0 41.0 20-21 39.29519994685345 41.0 39.0 41.0 36.0 41.0 22-23 39.25732843204055 41.0 39.0 41.0 36.0 41.0 24-25 39.20912979340983 41.0 39.0 41.0 36.0 41.0 26-27 39.08544874764929 40.5 39.0 41.0 35.5 41.0 28-29 38.955800298438305 40.0 38.5 41.0 35.0 41.0 30-31 38.81698304761386 40.0 38.0 41.0 35.0 41.0 32-33 38.71676041127252 40.0 38.0 41.0 35.0 41.0 34-35 38.672795610640215 40.0 38.0 41.0 35.0 41.0 36-37 38.59197674497833 40.0 38.0 41.0 35.0 41.0 38-39 38.476604105911534 40.0 38.0 41.0 34.0 41.0 40-41 38.35179369600175 40.0 38.0 41.0 34.0 41.0 42-43 38.22108044207026 40.0 38.0 41.0 34.0 41.0 44-45 38.05198124199395 40.0 38.0 41.0 33.0 41.0 46-47 37.84002820855251 40.0 38.0 41.0 33.0 41.0 48-49 37.59971825515794 40.0 37.0 41.0 33.0 41.0 50-51 37.38095820500941 40.0 37.0 41.0 32.5 41.0 52-53 37.17947844839334 40.0 37.0 41.0 32.0 41.0 54-55 36.962812235970674 39.5 36.0 41.0 31.0 41.0 56-57 36.699291379357334 39.0 36.0 41.0 31.0 41.0 58-59 36.413732420757135 39.0 35.0 40.5 31.0 41.0 60-61 36.05217815676869 38.5 35.0 40.0 30.0 41.0 62-63 35.684347149164644 38.0 35.0 40.0 29.0 41.0 64-65 35.28615191736393 37.5 34.5 40.0 29.0 41.0 66-67 34.91647968847946 37.0 34.0 39.5 28.5 41.0 68-69 34.45207254503829 36.0 34.0 39.0 28.0 40.5 70-71 34.021924995230435 36.0 34.0 39.0 27.5 40.0 72-73 33.552908921806434 35.0 33.0 38.0 26.0 40.0 74-75 33.073455343272194 35.0 33.0 37.0 26.0 39.0 76-77 31.076567993513393 33.5 30.0 35.5 23.0 37.5 78-79 32.056900534190945 34.5 32.0 36.0 25.0 38.5 80-81 32.08067782289935 35.0 32.0 36.0 25.0 37.5 82-83 31.79834802812679 35.0 32.0 35.5 25.0 37.0 84-85 31.436568913357497 34.5 32.0 35.0 24.0 37.0 86-87 30.923292156114577 34.0 31.0 35.0 21.5 36.0 88-89 30.39545716251942 34.0 31.0 35.0 19.0 36.0 90-91 29.96616217192227 34.0 30.0 35.0 16.5 35.0 92-93 29.716482584284975 34.0 30.0 35.0 11.0 35.0 94-95 29.41363788122428 34.0 30.0 35.0 4.5 35.0 96-97 28.887674600038157 34.0 29.5 35.0 2.0 35.0 98-99 28.168043423455345 34.0 29.0 35.0 2.0 35.0 100-101 24.531510111471476 30.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0569085745926472 1101 2 0.11598042438463807 1101 3 0.19199363914827927 1101 4 0.1374997953298518 1101 5 0.13849640474011693 1101 6 0.14441705262109394 1101 7 0.1381957273729455 1101 8 0.1497896912626615 1101 9 0.25611856166884195 1101 10-11 0.22860183943951284 1101 12-13 0.23198843387243784 1101 14-15 0.32026590093343543 1101 16-17 0.23458054992518385 1101 18-19 0.25553804626562027 1101 20-21 0.2987475023972834 1101 22-23 0.3269040399608514 1101 24-25 0.24716414048015878 1101 26-27 0.3080346604900228 1101 28-29 0.13550738011902297 1101 30-31 0.1295006719673637 1101 32-33 0.21120795695373573 1101 34-35 0.33848254194695926 1101 36-37 0.4309406125249424 1101 38-39 0.42452234610878037 1101 40-41 0.38751298139042945 1101 42-43 0.3736634744812477 1101 44-45 0.48538553247149707 1101 46-47 0.3763911033709064 1101 48-49 0.2704359122991846 1101 50-51 0.30380847329232097 1101 52-53 0.28482865836488 1101 54-55 0.3651062125036404 1101 56-57 0.283175802256018 1101 58-59 0.36164545859173813 1101 60-61 0.4083146211391693 1101 62-63 0.362140288806053 1101 64-65 0.34907356495062913 1101 66-67 0.3119552419187599 1101 68-69 0.36490855229170904 1101 70-71 0.33928371309514205 1101 72-73 0.37758544923748616 1101 74-75 0.26807332523201666 1101 76-77 0.23136969796304996 1101 78-79 0.344114664554823 1101 80-81 0.22811443506584794 1101 82-83 0.2433688033849677 1101 84-85 0.20791249557549918 1101 86-87 0.4085007796978388 1101 88-89 0.5270238301698065 1101 90-91 0.6533883503985578 1101 92-93 0.6481169801941284 1101 94-95 0.5438284668319788 1101 96-97 0.5800354127875131 1101 98-99 0.5729307340776693 1101 100-101 0.03019500783965512 1102 1 0.15666025984117482 1102 2 0.13885412496617988 1102 3 0.16481199965073046 1102 4 0.11262061321805561 1102 5 0.091217013671816 1102 6 0.0845477935579666 1102 7 0.15760411330320778 1102 8 0.14101816945819223 1102 9 0.24983555441261274 1102 10-11 0.20271514586612227 1102 12-13 0.20765340932720022 1102 14-15 0.27975493548758834 1102 16-17 0.30394707299388557 1102 18-19 0.281296229202745 1102 20-21 0.2777367587888264 1102 22-23 0.3115155621469725 1102 24-25 0.3421081563570354 1102 26-27 0.24302736132441538 1102 28-29 0.20898683988535538 1102 30-31 0.30030442698255655 1102 32-33 0.20947708863926806 1102 34-35 0.20167149015150443 1102 36-37 0.3648691498771157 1102 38-39 0.3693796545772443 1102 40-41 0.4217606187030043 1102 42-43 0.2960481190405062 1102 44-45 0.2816779857067431 1102 46-47 0.22900878452617235 1102 48-49 0.24130693413466275 1102 50-51 0.29555844824616173 1102 52-53 0.2994010489131753 1102 54-55 0.17729283737900658 1102 56-57 0.25788842742716156 1102 58-59 0.18456346701679394 1102 60-61 0.19455074437579611 1102 62-63 0.24557905453146134 1102 64-65 0.18300204266909503 1102 66-67 0.2204228703578437 1102 68-69 0.26635550453226386 1102 70-71 0.2385072226102949 1102 72-73 0.19670568510071718 1102 74-75 0.24261837968951738 1102 76-77 0.27273461807773813 1102 78-79 0.3631154541050847 1102 80-81 0.3505284194084197 1102 82-83 0.29878968615464174 1102 84-85 0.32636859085585 1102 86-87 0.434795964156816 1102 88-89 0.44675119679306263 1102 90-91 0.5253336138125633 1102 92-93 0.4214316720309874 1102 94-95 0.4103566043997837 1102 96-97 0.47318701836541166 1102 98-99 0.43153325395954667 1102 100-101 0.2660492964572505 1103 1 0.12584788322906348 1103 2 0.057430805347742364 1103 3 0.11631710122086503 1103 4 0.04940911485092414 1103 5 0.11478121688589482 1103 6 0.06770131115054312 1103 7 0.0957971611454198 1103 8 0.1332902824123039 1103 9 0.1210197552281258 1103 10-11 0.17881566201955224 1103 12-13 0.1630403283663 1103 14-15 0.24039794781253931 1103 16-17 0.22304844017778436 1103 18-19 0.21263923137991014 1103 20-21 0.17722637114329842 1103 22-23 0.1650480881160874 1103 24-25 0.10032209941871884 1103 26-27 0.12030946062514403 1103 28-29 0.12415824526183883 1103 30-31 0.12019299734362932 1103 32-33 0.11838338764847833 1103 34-35 0.19119303344566418 1103 36-37 0.21269613930495979 1103 38-39 0.23737422089765658 1103 40-41 0.2839398102010051 1103 42-43 0.1357257422438778 1103 44-45 0.08227249621431554 1103 46-47 0.08123853046244278 1103 48-49 0.1982710847647482 1103 50-51 0.1992911047527386 1103 52-53 0.2459077879674041 1103 54-55 0.33286333914102784 1103 56-57 0.2933033451378222 1103 58-59 0.23962877034342256 1103 60-61 0.2525531406377226 1103 62-63 0.09297015030814038 1103 64-65 0.1705880105836286 1103 66-67 0.18651657134863342 1103 68-69 0.21361294931907082 1103 70-71 0.18455615801500613 1103 72-73 0.17260958784800096 1103 74-75 0.10128995342704172 1103 76-77 0.09567329083922615 1103 78-79 0.19605748255580124 1103 80-81 0.07054314523361427 1103 82-83 0.2341514432903864 1103 84-85 0.19579451430461958 1103 86-87 0.31300606720300195 1103 88-89 0.312474042233017 1103 90-91 0.4214764739206842 1103 92-93 0.44811545540337505 1103 94-95 0.25721061692769 1103 96-97 0.26953992616813593 1103 98-99 0.19598779878510086 1103 100-101 -0.051844599186583906 1104 1 0.041902528136290584 1104 2 0.042844224168874234 1104 3 0.10402672806031177 1104 4 0.09646194847412204 1104 5 0.12423160257224453 1104 6 0.06338317848525321 1104 7 0.06800499021787942 1104 8 0.07808413621194887 1104 9 0.09213551970713496 1104 10-11 0.10880088901579654 1104 12-13 0.031278849015841104 1104 14-15 0.061066607867864775 1104 16-17 0.12038873629118285 1104 18-19 0.13895852313180512 1104 20-21 0.13720634338829996 1104 22-23 0.1710289581181641 1104 24-25 0.14933096209455243 1104 26-27 0.21002265145877885 1104 28-29 0.165343554937337 1104 30-31 0.24010087304989725 1104 32-33 0.23075452515286798 1104 34-35 0.225371867330189 1104 36-37 0.2206892185779239 1104 38-39 0.24050735928516076 1104 40-41 0.3231831710790658 1104 42-43 0.18188814052811608 1104 44-45 0.0437513116255559 1104 46-47 0.042204683781776 1104 48-49 -0.0502702007963407 1104 50-51 0.028300203976861837 1104 52-53 -0.048773649074711045 1104 54-55 0.001263613762326088 1104 56-57 -0.01608956494679603 1104 58-59 0.007672476581987553 1104 60-61 -0.04806016711835781 1104 62-63 0.018073330418253875 1104 64-65 -0.05004381552409143 1104 66-67 0.052455082310956413 1104 68-69 -0.0055662168594778905 1104 70-71 0.08797321066839459 1104 72-73 0.06783038314587486 1104 74-75 0.05605896402440891 1104 76-77 0.17256936616521656 1104 78-79 0.24476390134989856 1104 80-81 0.1451097831390129 1104 82-83 0.21710996961708418 1104 84-85 0.14407534330499416 1104 86-87 0.09585544339137542 1104 88-89 0.07627550838182984 1104 90-91 0.025857489005705503 1104 92-93 0.15499453304527933 1104 94-95 0.0654030444538023 1104 96-97 0.17049124453059505 1104 98-99 0.012678786745919268 1104 100-101 -0.21873026075581947 1105 1 0.029504068304760267 1105 2 0.0940615609622526 1105 3 0.08629709410527653 1105 4 0.02284203720750355 1105 5 0.10948528836686222 1105 6 0.03976025417748019 1105 7 0.07956361595481098 1105 8 0.08334400623944305 1105 9 0.13421352557016064 1105 10-11 0.1380837634079768 1105 12-13 0.14991141369192462 1105 14-15 0.14193956360124815 1105 16-17 0.16193230227582944 1105 18-19 0.19361236287782901 1105 20-21 0.16298447830764218 1105 22-23 0.18520804346793796 1105 24-25 0.09056643439625134 1105 26-27 0.1334884068145641 1105 28-29 0.21145658188210348 1105 30-31 0.22812689754523774 1105 32-33 0.14225823446556518 1105 34-35 0.21294123203659865 1105 36-37 0.17000148170994578 1105 38-39 0.26659784159260624 1105 40-41 0.2214878514363008 1105 42-43 0.3035595821606023 1105 44-45 0.34110323019654487 1105 46-47 0.3428183898196693 1105 48-49 0.36871205389098094 1105 50-51 0.3014505171013653 1105 52-53 0.2681543114290932 1105 54-55 0.27345561619674896 1105 56-57 0.32121180270121386 1105 58-59 0.2683817928234262 1105 60-61 0.4309551601265511 1105 62-63 0.3312464417663463 1105 64-65 0.1458513726355548 1105 66-67 0.15607956631061626 1105 68-69 0.20601982618855885 1105 70-71 0.3141210361934128 1105 72-73 0.1756653535648951 1105 74-75 0.2102653876100007 1105 76-77 0.18745133539074743 1105 78-79 0.4263095151118321 1105 80-81 0.31263480296918544 1105 82-83 0.25964068150463504 1105 84-85 0.3740898822510701 1105 86-87 0.4391658512535308 1105 88-89 0.5298606319751435 1105 90-91 0.543017985391284 1105 92-93 0.5753875999421574 1105 94-95 0.3442254416293409 1105 96-97 0.2837075609800799 1105 98-99 0.217656248925433 1105 100-101 0.0866403010413812 1106 1 0.06012890453054354 1106 2 0.08180506899763174 1106 3 0.01999283198117041 1106 4 0.02652098846959916 1106 5 0.04961880062556645 1106 6 0.08550969672710806 1106 7 0.10427969943562232 1106 8 0.11305274769097906 1106 9 0.1391710542142235 1106 10-11 0.10600958224004842 1106 12-13 0.1897234796007936 1106 14-15 0.12733044761904466 1106 16-17 0.07902910803121443 1106 18-19 0.03932566125357795 1106 20-21 0.12597371598304363 1106 22-23 0.134196227783967 1106 24-25 0.12324731204653716 1106 26-27 0.1812260391447822 1106 28-29 0.19208412436712052 1106 30-31 0.16485384093211763 1106 32-33 0.14983198718581292 1106 34-35 0.1693743615417702 1106 36-37 0.28828758431411927 1106 38-39 0.34087548505493004 1106 40-41 0.2556220423415567 1106 42-43 0.240551361637948 1106 44-45 0.20615574333556452 1106 46-47 0.31894632093912634 1106 48-49 0.32961552032282526 1106 50-51 0.28080963067656484 1106 52-53 0.3819651329791398 1106 54-55 0.4173494366359307 1106 56-57 0.331267702567537 1106 58-59 0.28016202219451003 1106 60-61 0.3357350384645841 1106 62-63 0.33803284218915053 1106 64-65 0.4665363919033396 1106 66-67 0.4778731109975993 1106 68-69 0.39095803630512904 1106 70-71 0.37475028570348456 1106 72-73 0.2875429562251526 1106 74-75 0.1831886133773324 1106 76-77 0.18401367055939133 1106 78-79 0.3981786008619963 1106 80-81 0.3645219243758788 1106 82-83 0.3559481859354747 1106 84-85 0.2836807218758075 1106 86-87 0.4062039389899361 1106 88-89 0.6694442141412047 1106 90-91 0.716637981836719 1106 92-93 0.6558722051002341 1106 94-95 0.6436916319943826 1106 96-97 0.5040945794708591 1106 98-99 0.7817485344472352 1106 100-101 0.4288471721815448 1107 1 -0.012419170263562762 1107 2 -0.044642841500433406 1107 3 0.006076454089757988 1107 4 -0.007599149542080852 1107 5 0.013606143300279427 1107 6 0.07027759947946066 1107 7 0.0659590917278905 1107 8 0.037788796975291916 1107 9 0.06407034876948359 1107 10-11 0.07513840039682407 1107 12-13 0.06854820371628279 1107 14-15 0.04855691121610306 1107 16-17 0.07244919778233339 1107 18-19 0.15208160919822689 1107 20-21 0.055548017349572376 1107 22-23 0.08999241062167584 1107 24-25 0.0021367713556728063 1107 26-27 0.12856461584116374 1107 28-29 0.07877745818080939 1107 30-31 0.06706823488686098 1107 32-33 0.1810005756433597 1107 34-35 0.1989851773102842 1107 36-37 0.15607111132764118 1107 38-39 0.12430548961078358 1107 40-41 0.09886248723933022 1107 42-43 0.05641032072605867 1107 44-45 -0.022317459296473885 1107 46-47 0.06347610325624942 1107 48-49 0.08566873002507691 1107 50-51 0.0938223052645597 1107 52-53 0.13592223587469476 1107 54-55 0.104216298296258 1107 56-57 0.13945206990017311 1107 58-59 0.07364418267624018 1107 60-61 0.11046144314322959 1107 62-63 0.011233635373528728 1107 64-65 0.07045964485995881 1107 66-67 0.0702716231013838 1107 68-69 -0.04356785064207713 1107 70-71 -0.033689194583615745 1107 72-73 -0.1311813514085003 1107 74-75 -0.14939529281554798 1107 76-77 -0.15247367399170386 1107 78-79 0.13820356425377867 1107 80-81 -0.0037633472980260763 1107 82-83 -0.023356704442505816 1107 84-85 0.13880729888141374 1107 86-87 0.05626518411227721 1107 88-89 0.10767614401553871 1107 90-91 0.2145718971488222 1107 92-93 0.27386119598927294 1107 94-95 0.09225736279654839 1107 96-97 0.17465605313658017 1107 98-99 0.34340270815971863 1107 100-101 0.0038045220090303644 1108 1 -0.0358768889298986 1108 2 -0.04956691107541644 1108 3 0.022303482423204457 1108 4 0.020958433911040686 1108 5 0.02873234730810026 1108 6 0.003481912113571184 1108 7 0.005270905178150542 1108 8 0.0481197116214247 1108 9 -0.036503390528139334 1108 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2316 84-85 -0.10781462389426366 2316 86-87 -0.2838541617787733 2316 88-89 -0.4641962190590654 2316 90-91 -0.5073976129618245 2316 92-93 -0.6141843094998585 2316 94-95 -0.2429055369883848 2316 96-97 -0.34441765312021033 2316 98-99 -0.2973813052028724 2316 100-101 0.10128778632253344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 21.0 5 43.0 6 86.0 7 196.0 8 477.0 9 946.0 10 1644.0 11 2635.0 12 3779.0 13 5532.0 14 6844.0 15 8541.0 16 11049.0 17 15201.0 18 15244.0 19 11296.0 20 10896.0 21 12071.0 22 13113.0 23 14315.0 24 16654.0 25 19846.0 26 23881.0 27 29262.0 28 36067.0 29 44446.0 30 55412.0 31 68966.0 32 85770.0 33 112957.0 34 157880.0 35 236818.0 36 397601.0 37 726524.0 38 713988.0 39 75267.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24678658932853 19.062595827993174 14.064105145593597 23.626512437084703 2 15.80929246954294 20.435597285437844 39.74544166144286 24.009668583576353 3 20.403096127115642 23.274202120411 29.262114687525553 27.060587064947804 4 13.31607206126843 16.189869450273907 36.70222261590036 33.7918358725573 5 14.204982148210732 37.06102995284947 34.105264233735795 14.628723665204003 6 33.04410482134583 35.52577607587692 16.993710991796355 14.436408110980894 7 29.559088059742173 31.83243165898994 20.375330462511243 18.233149818756644 8 25.823771497097383 35.38640266005287 19.336008830503392 19.453817012346352 9 25.58617917200403 17.789205799787414 20.50789021830967 36.116724809898884 10-11 25.48687007713063 26.01542271946799 27.20495148674062 21.29275571666076 12-13 26.481442996920222 23.201040445885912 27.53796912049276 22.779547436701097 14-15 22.689415458151313 25.23211415732404 26.001861853216806 26.076608531307837 16-17 22.629566008976493 28.376831462833135 26.43982295035946 22.55377957783091 18-19 22.377204623954892 27.626534372621077 27.974848732463126 22.02141227096091 20-21 23.284780327600775 26.503587392003485 27.71437477855605 22.497257501839687 22-23 22.776464255539505 26.453285546864354 27.775936196887518 22.99431400070862 24-25 23.09191057982479 26.571697420267874 27.80432846631276 22.53206353359457 26-27 22.890902540420424 26.439944910854894 28.31033551742318 22.358817031301502 28-29 22.3065431577226 27.40932721920907 27.95266550380202 22.331464119266307 30-31 22.726398540198957 26.49262255022623 27.78865250667375 22.992326402901067 32-33 22.630498623640673 26.71515153579897 27.919040091575592 22.735309748984765 34-35 23.343913768319137 26.588300351246364 27.635054721042106 22.43273115939239 36-37 23.097372561093948 26.340006450985204 28.034846906670808 22.52777408125004 38-39 22.679393261597156 26.841774908148935 28.040830874771096 22.438000955482813 40-41 22.94171211866955 26.836242460277237 27.688630426023618 22.533414995029595 42-43 22.662325018181534 26.908788413972346 27.186030177000998 23.242856390845127 44-45 22.94356262615233 26.578419159363197 27.466538384292814 23.011479830191657 46-47 23.26617522432171 27.050434590045953 27.16124334061554 22.5221468450168 48-49 23.289365561300272 26.843041969763455 27.24379066084866 22.623801808087606 50-51 22.510862257192972 27.10955706587978 27.682994986676736 22.69658569025052 52-53 23.03768945826919 27.337641387848578 26.797402766416933 22.827266387465308 54-55 22.71008217273991 26.67651808345371 27.250313428361178 23.363086315445205 56-57 22.99419476165817 26.841715270774852 27.175380202229434 22.988709765337546 58-59 23.52003652295972 26.608577444346775 27.001556995577726 22.869829037115778 60-61 23.028534601848293 26.75579957004825 27.785210157273614 22.43045567082984 62-63 22.706363177660585 26.874936332109435 27.48858407040451 22.930116419825474 64-65 22.50924206388548 27.22357192184166 27.112524065479228 23.154661948793635 66-67 22.714051338694436 26.896028812111933 26.917782351831903 23.47213749736172 68-69 22.452840570234873 26.84998670437706 27.26474881493498 23.432423910453085 70-71 22.984190426904977 26.719951786517022 27.064280271871986 23.231577514706014 72-73 22.961693603335966 26.331355100705895 27.57559755798425 23.13135373797389 74-75 22.51199204164509 27.290854705513613 26.91935692676678 23.277796326074515 76-77 22.678561643122368 26.715573345251297 26.982908155470042 23.62295685615629 78-79 22.775246583483902 26.748467386318126 27.200811784018708 23.27547424617926 80-81 22.76647428777792 26.769635933741714 27.13147064234561 23.332419136134753 82-83 22.7478996749862 26.663992286884298 27.303203123403037 23.284904914726464 84-85 22.54699722002671 26.790953503583985 27.193197923196422 23.468851353192882 86-87 22.48894509492357 26.839745783652123 27.132525337479212 23.538783783945092 88-89 22.395065356084316 26.81476596136976 26.80176709346853 23.988401589077395 90-91 22.577146471966536 26.46294022712165 27.020679962601868 23.939233338309947 92-93 22.778849036548472 26.66002902619171 26.996623831457306 23.56449810580251 94-95 22.546690605325555 26.6471001062931 27.042275353628952 23.76393393475239 96-97 22.050711014061864 26.606086405059553 27.5097141515825 23.833488429296086 98-99 22.238098304291345 26.77964577258432 26.711201461689754 24.271054461434577 100-101 22.151687099556273 26.01768259331074 27.3328133338807 24.497816973252277 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 199.0 1 145.5 2 115.5 3 149.5 4 138.0 5 139.5 6 259.5 7 546.0 8 911.0 9 1119.0 10 1062.0 11 1031.5 12 1156.0 13 1311.5 14 1466.0 15 1617.0 16 1765.0 17 1953.5 18 2089.0 19 2276.5 20 2644.5 21 3155.0 22 3899.0 23 4952.5 24 6289.0 25 8329.0 26 11308.5 27 15088.5 28 19280.5 29 23823.0 30 28908.0 31 35159.5 32 41281.5 33 46034.0 34 50897.0 35 56046.5 36 61890.0 37 68503.5 38 72630.0 39 75886.0 40 81903.5 41 89300.0 42 95883.5 43 101695.5 44 106456.0 45 109412.0 46 111238.5 47 122822.0 48 148179.5 49 147345.5 50 124023.0 51 113744.5 52 109419.5 53 106571.0 54 102273.0 55 93126.5 56 83596.5 57 75722.0 58 68607.0 59 59945.0 60 50166.0 61 40798.0 62 32765.0 63 26186.5 64 20065.0 65 14991.5 66 10985.0 67 8547.0 68 6353.0 69 4305.0 70 3199.0 71 2451.0 72 1973.0 73 1745.5 74 1402.0 75 1048.0 76 693.5 77 393.0 78 225.5 79 136.5 80 86.5 81 55.0 82 36.5 83 16.5 84 10.5 85 8.0 86 4.0 87 3.0 88 3.5 89 2.5 90 1.0 91 1.0 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3446689923959554 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011072197541631462 16-17 0.001975961407429615 18-19 1.7034150064048403E-5 20-21 0.0 22-23 0.0 24-25 4.769562017933553E-4 26-27 0.0012775612548036304 28-29 0.0 30-31 0.0029128396609522773 32-33 0.0 34-35 0.010322694938813333 36-37 0.028770679458177753 38-39 0.021735575481725763 40-41 0.01388283230219945 42-43 0.010101250987980703 44-45 0.0030150445613365677 46-47 0.0014479027554441144 48-49 8.176392030743234E-4 50-51 5.110245019214521E-5 52-53 1.0220490038429043E-4 54-55 0.0 56-57 0.0 58-59 0.004769562017933553 60-61 0.010203455888364993 62-63 0.0043266741162682944 64-65 0.0020100297075577116 66-67 0.00427557166607615 68-69 0.0035260690632580196 70-71 5.110245019214521E-5 72-73 0.0 74-75 0.0 76-77 0.21696396936578452 78-79 5.621269521135973E-4 80-81 0.003406830012809681 82-83 6.813660025619361E-4 84-85 0.0 86-87 0.1093762775612548 88-89 0.4070650840805647 90-91 0.3953455888364994 92-93 0.0 94-95 0.0 96-97 1.7034150064048403E-5 98-99 0.00160121010602055 100-101 6.984001526259845E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2935280.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.64161227311961 #Duplication Level Percentage of deduplicated Percentage of total 1 72.57924228742782 36.029506047092994 2 14.70480553298264 14.599405096398964 3 5.268121453568768 7.845541278172923 4 2.5107804420624333 4.985567568311807 5 1.3187743225397537 3.273304179763222 6 0.8013049423910991 2.386684155762804 7 0.5170012948403162 1.7965344476214642 8 0.3585459008526043 1.4239037273793096 9 0.28037190342562085 1.2526301989918087 >10 1.4821891576463857 13.707568400831086 >50 0.11672918408799095 3.995504355624209 >100 0.05822179760245531 4.996376028030412 >500 0.0019215764215671039 0.6139037618217461 >1k 0.0017156932333380064 1.9212598581916565 >5k 2.0588318800056075E-4 0.6203766649112522 >10k+ 6.862772933352026E-5 0.5519342310942996 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16201 0.5519405303752963 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7151 0.24362241421602027 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5626 0.19166825652067265 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5433 0.18509307459594998 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4622 0.15746368319206344 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4542 0.1547382191818157 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3694 0.1258483006731896 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3561 0.12131721675615273 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3504 0.11937532364885123 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3439 0.11716088414052493 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3312 0.11283421002425663 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.4068300128096807E-5 0.0 9 0.0 0.0 0.0 3.4068300128096807E-5 0.0 10-11 0.0 0.0 0.0 3.4068300128096807E-5 0.0 12-13 0.0 1.7034150064048403E-5 0.0 5.110245019214521E-5 0.0 14-15 0.0 3.4068300128096807E-5 0.0 6.813660025619361E-5 0.0 16-17 0.0 3.4068300128096807E-5 0.0 6.813660025619361E-5 0.0 18-19 0.0 3.4068300128096807E-5 0.0 6.813660025619361E-5 0.0 20-21 0.0 3.4068300128096807E-5 0.0 8.517075032024203E-5 0.0 22-23 0.0 3.4068300128096807E-5 0.0 1.7034150064048405E-4 0.0 24-25 0.0 3.4068300128096807E-5 0.0 3.236488512169197E-4 0.0 26-27 0.0 3.4068300128096807E-5 0.0 6.30263552369791E-4 0.0 28-29 0.0 3.4068300128096807E-5 0.0 0.0020781663078139057 0.0 30-31 0.0 3.4068300128096807E-5 0.0 0.006847728325747458 0.0 32-33 0.0 3.4068300128096807E-5 0.0 0.017579242866097952 0.0 34-35 0.0 3.4068300128096807E-5 0.0 0.035260690632580194 0.0 36-37 0.0 3.4068300128096807E-5 0.0 0.0639802676405658 0.0 38-39 0.0 3.4068300128096807E-5 0.0 0.10770693085497807 0.0 40-41 0.0 3.4068300128096807E-5 0.0 0.15657790738873292 0.0 42-43 0.0 3.4068300128096807E-5 0.0 0.20926453353683466 0.0 44-45 0.0 3.4068300128096807E-5 0.0 0.27551034313591893 0.0 46-47 0.0 3.4068300128096807E-5 0.0 0.35700171704232647 0.0 48-49 0.0 3.4068300128096807E-5 0.0 0.4433648578670519 0.0 50-51 0.0 3.4068300128096807E-5 0.0 0.538091766373225 0.0 52-53 0.0 3.4068300128096807E-5 0.0 0.6349990460875965 0.0 54-55 0.0 3.4068300128096807E-5 0.0 0.7475947780109564 0.0 56-57 0.0 3.4068300128096807E-5 0.0 0.8881605843394837 0.0 58-59 0.0 3.4068300128096807E-5 0.0 1.04020740781118 0.0 60-61 0.0 3.4068300128096807E-5 0.0 1.203616009375596 0.0 62-63 0.0 3.4068300128096807E-5 0.0 1.372134855959227 0.0 64-65 0.0 3.4068300128096807E-5 0.0 1.5560696083508216 0.0 66-67 0.0 3.4068300128096807E-5 0.0 1.7774113542830667 0.0 68-69 0.0 3.4068300128096807E-5 0.0 2.0260418086179173 0.0 70-71 0.0 3.4068300128096807E-5 0.0 2.2893216320078493 0.0 72-73 0.0 3.4068300128096807E-5 0.0 2.563111525987299 0.0 74-75 0.0 3.4068300128096807E-5 0.0 2.858296312447194 0.0 76-77 0.0 3.4068300128096807E-5 0.0 3.183171622468725 0.0 78-79 0.0 3.4068300128096807E-5 0.0 3.5582125044288793 0.0 80-81 0.0 3.4068300128096807E-5 0.0 3.9658056471614294 0.0 82-83 0.0 3.4068300128096807E-5 0.0 4.371661306587447 0.0 84-85 0.0 3.4068300128096807E-5 0.0 4.792421847319506 0.0 86-87 0.0 3.4068300128096807E-5 0.0 5.2467055953776125 0.0 88-89 0.0 3.4068300128096807E-5 0.0 5.7531819792319645 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8980 0.0 24.757992 1 TACATGG 9295 0.0 23.941608 2 ACATGGG 9360 0.0 23.217716 3 GAGTACT 6240 0.0 19.163527 12-13 CATGGGG 5330 0.0 18.961918 4 CATGGGA 4240 0.0 17.793491 4 GTACTTT 6785 0.0 17.239574 14-15 GTGTAGC 1635 0.0 17.179445 1 AGAGTAC 11100 0.0 16.672514 10-11 GAGTACA 7760 0.0 16.012299 1 AGTACTT 6825 0.0 15.88704 12-13 GTATAGG 900 0.0 15.869148 1 AGTACAT 7000 0.0 15.590487 2 ATGGGAG 2450 0.0 15.29998 5 TACACTG 2290 0.0 14.504155 5 GGACAGT 2245 0.0 14.372173 6 CCTACAC 1100 0.0 14.234793 3 ATGGGGA 3395 0.0 14.1159935 5 GAACCGC 505 7.7852746E-10 14.093853 6 GTAAGCG 390 0.0 13.991701 94-95 >>END_MODULE