FastQCFastQC Report
Fri 27 May 2016
SRR939104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881192
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT142510.4946216704752755No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT77760.269888296232948No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT64910.22528869995474096No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57020.19790420076135157No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41600.14438468522750306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41000.14230221380595254No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35110.12185928601773155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33160.11509125389769234No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30520.10592837964287004No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30490.10582425607179251No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29120.10106927965925214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG84750.026.5626431
TACATGG86850.025.1939072
ACATGGG86350.024.352953
GAGTACT55750.019.3187412-13
CATGGGG50800.018.9261914
TAGCCCT17800.017.8273074
GTGTAGC17500.017.640381
AGTACTT61900.017.0934312-13
AGAGTAC102750.017.06911310-11
GTACTTT62000.017.02856614-15
CATGGGA39550.016.8850984
GTATTAG10300.016.5996111
TACCTGG22250.015.9644762
GTAGCCC16500.015.7873673
AGGACCG3956.7993824E-915.5869855
GTATAGC10150.015.4411831
GAGTACA72100.015.2821831
CGATCGT2851.8189894E-1214.95398324-25
TACTTTT73400.014.48055514-15
AGTACAT64550.014.3074342
Produced by FastQC (version 0.11.5)