##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939104_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2881192 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14958739299568 33.0 31.0 34.0 30.0 34.0 2 32.25409309757906 34.0 31.0 34.0 30.0 34.0 3 32.32927621623273 34.0 31.0 34.0 30.0 34.0 4 35.745920438485186 37.0 35.0 37.0 35.0 37.0 5 35.785097973338814 37.0 35.0 37.0 35.0 37.0 6 35.865574040188925 37.0 37.0 37.0 35.0 37.0 7 35.79909287544877 37.0 36.0 37.0 35.0 37.0 8 35.79487725913442 37.0 36.0 37.0 35.0 37.0 9 37.483656417205104 39.0 38.0 39.0 35.0 39.0 10-11 37.387168748212545 39.0 38.0 39.0 35.0 39.0 12-13 37.298501627104336 39.0 37.0 39.0 34.0 39.0 14-15 38.61011466781804 40.0 38.0 41.0 34.0 41.0 16-17 38.52706414567304 40.0 38.0 41.0 34.0 41.0 18-19 38.407798057193 40.0 38.0 41.0 34.0 41.0 20-21 38.3855343552252 40.0 38.0 41.0 34.0 41.0 22-23 38.43937474489725 40.0 38.0 41.0 34.0 41.0 24-25 38.38886526826397 40.0 38.0 41.0 34.0 41.0 26-27 38.28246729131554 40.0 38.0 41.0 34.0 41.0 28-29 38.15760889937221 40.0 38.0 41.0 33.5 41.0 30-31 37.993179385476566 40.0 38.0 41.0 33.0 41.0 32-33 37.83177205823145 40.0 38.0 41.0 33.0 41.0 34-35 37.52757140100347 40.0 38.0 41.0 32.5 41.0 36-37 37.284424293833936 40.0 37.0 41.0 31.0 41.0 38-39 37.12232142113403 40.0 37.0 41.0 31.0 41.0 40-41 36.97134467262161 40.0 37.0 41.0 30.5 41.0 42-43 36.79169558988086 40.0 37.0 41.0 30.0 41.0 44-45 36.57168265773333 40.0 36.0 41.0 30.0 41.0 46-47 36.51483257623928 40.0 36.0 41.0 30.0 41.0 48-49 36.447340892241826 40.0 36.0 41.0 30.0 41.0 50-51 35.33780706041111 38.5 34.5 40.0 28.0 40.5 52-53 35.49824569136663 38.5 35.0 40.0 28.0 40.5 54-55 36.05389713701829 39.0 36.0 41.0 29.0 41.0 56-57 35.96805870625769 39.0 35.0 41.0 28.5 41.0 58-59 35.77324298415378 39.0 35.0 41.0 28.0 41.0 60-61 35.46746121049898 39.0 35.0 41.0 28.0 41.0 62-63 35.109347277099204 38.0 35.0 40.0 27.0 41.0 64-65 34.73789025514439 37.5 34.5 40.0 26.0 41.0 66-67 34.33865011425827 37.0 34.0 40.0 26.0 41.0 68-69 33.92940022740588 36.5 34.0 39.0 26.0 41.0 70-71 33.48687869465138 36.0 33.5 39.0 25.0 41.0 72-73 33.034454836748125 35.5 33.0 38.5 24.0 40.0 74-75 32.59685574581631 35.0 33.0 37.5 23.0 39.5 76-77 32.157246202266286 35.0 32.5 37.0 22.0 39.0 78-79 31.72209175924409 35.0 32.0 36.5 20.5 39.0 80-81 31.26625056573807 35.0 31.5 36.0 19.5 37.5 82-83 30.869175153894638 35.0 31.0 35.5 17.5 37.0 84-85 30.487935548897816 34.5 31.0 35.0 13.0 37.0 86-87 30.094900825769336 34.0 31.0 35.0 7.0 36.0 88-89 29.67732105323075 34.0 30.0 35.0 2.0 36.0 90-91 29.26195564197041 34.0 30.0 35.0 2.0 35.5 92-93 28.857141419245924 34.0 29.0 35.0 2.0 35.0 94-95 28.37155541872947 34.0 29.0 35.0 2.0 35.0 96-97 27.77463008365982 34.0 28.5 35.0 2.0 35.0 98-99 27.060697794523932 34.0 27.0 35.0 2.0 35.0 100-101 24.01759080963712 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1374911738196758 1101 2 0.35821928791327906 1101 3 0.24392645092726895 1101 4 0.2887163502906631 1101 5 0.2245845659714547 1101 6 0.1875820589004107 1101 7 0.1449860499796003 1101 8 0.23535628983589163 1101 9 0.2254134054440513 1101 10-11 0.2868983013493249 1101 12-13 0.33681968499260506 1101 14-15 0.43376705921155434 1101 16-17 0.4544757567215356 1101 18-19 0.526176394959883 1101 20-21 0.5014816943306144 1101 22-23 0.49404837089505094 1101 24-25 0.5383036322787405 1101 26-27 0.5105125577997924 1101 28-29 0.5663373358080221 1101 30-31 0.5811803329432124 1101 32-33 0.6200983447463955 1101 34-35 0.5322397229296598 1101 36-37 0.5208712703326199 1101 38-39 0.43432545586720295 1101 40-41 0.4441105371364884 1101 42-43 0.4724961013597522 1101 44-45 0.5203241051591405 1101 46-47 0.5163453043980653 1101 48-49 0.5492537523536072 1101 50-51 0.5941166250638474 1101 52-53 0.4927506021565762 1101 54-55 0.5795617751036346 1101 56-57 0.5974450158442863 1101 58-59 0.6378352339624271 1101 60-61 0.4982081966072158 1101 62-63 0.5311936291107244 1101 64-65 0.46386712500939353 1101 66-67 0.4045351689693959 1101 68-69 0.35933768465421423 1101 70-71 0.4013781249834736 1101 72-73 0.389956478610074 1101 74-75 0.40681824829931656 1101 76-77 0.313736887026252 1101 78-79 0.2504570250041631 1101 80-81 0.11738961669792403 1101 82-83 0.024998692711101 1101 84-85 0.0635497170084669 1101 86-87 0.06510460781328575 1101 88-89 0.06356510689416339 1101 90-91 0.047462177971144826 1101 92-93 0.015522350339796986 1101 94-95 -0.05167003253266955 1101 96-97 -0.10385656248552166 1101 98-99 -0.02030234934424513 1101 100-101 -0.03767481513030546 1102 1 0.22938134701385593 1102 2 0.2931635293669004 1102 3 0.3167197420224426 1102 4 0.21356008117111713 1102 5 0.21877792947127261 1102 6 0.3085152418451216 1102 7 0.2647291846947084 1102 8 0.34820893751884796 1102 9 0.36516577143365225 1102 10-11 0.40363699224555205 1102 12-13 0.42786836031106645 1102 14-15 0.5732461642597784 1102 16-17 0.5168930572458024 1102 18-19 0.5174012950548033 1102 20-21 0.5156833380807626 1102 22-23 0.429540243759611 1102 24-25 0.5488474786135242 1102 26-27 0.5565757298964868 1102 28-29 0.583109756361047 1102 30-31 0.6237803505688575 1102 32-33 0.6342405494866767 1102 34-35 0.7074976248308786 1102 36-37 0.6503663652670184 1102 38-39 0.5441711236668638 1102 40-41 0.5705661588621922 1102 42-43 0.6368390990005821 1102 44-45 0.5673511402398859 1102 46-47 0.6579469368627358 1102 48-49 0.7125184471033847 1102 50-51 0.6225717433807674 1102 52-53 0.5481781921282192 1102 54-55 0.6299177117451364 1102 56-57 0.6492404860528183 1102 58-59 0.7364500989772367 1102 60-61 0.6791657447908577 1102 62-63 0.5596267153679761 1102 64-65 0.5955269094312357 1102 66-67 0.7062667620553285 1102 68-69 0.5859237868943765 1102 70-71 0.39747512183939193 1102 72-73 0.4273789622159967 1102 74-75 0.4315664329526996 1102 76-77 0.2380957540901889 1102 78-79 0.3229367951352806 1102 80-81 0.3022362196713324 1102 82-83 0.049695680563988986 1102 84-85 0.07705711312334529 1102 86-87 0.06185004464087385 1102 88-89 0.03737263355295539 1102 90-91 -0.06255451567290393 1102 92-93 -0.04650138468712228 1102 94-95 -0.1651205574029042 1102 96-97 -0.22734815891905313 1102 98-99 -0.30963845205992513 1102 100-101 -0.4326026545872814 1103 1 0.09581761516425757 1103 2 0.131084570325946 1103 3 0.19275658840258103 1103 4 0.2626401798752198 1103 5 0.2670530785385452 1103 6 0.2699896852247221 1103 7 0.21025076932068743 1103 8 0.1847539845318451 1103 9 0.2530456856814993 1103 10-11 0.32463065482097164 1103 12-13 0.3364622360844436 1103 14-15 0.3940984073406213 1103 16-17 0.43712118391425037 1103 18-19 0.43705807423149423 1103 20-21 0.4576409361838145 1103 22-23 0.4367722842114361 1103 24-25 0.5210263120459473 1103 26-27 0.5502955310208719 1103 28-29 0.48924861153772525 1103 30-31 0.49053533936172045 1103 32-33 0.4439806977975138 1103 34-35 0.45063000978902323 1103 36-37 0.4940248325640866 1103 38-39 0.4550299245859861 1103 40-41 0.3615483277876166 1103 42-43 0.3098695418623407 1103 44-45 0.4171847536633564 1103 46-47 0.43705503750599206 1103 48-49 0.4418197962959951 1103 50-51 0.26106886147915276 1103 52-53 0.47430670574854616 1103 54-55 0.28607301457446965 1103 56-57 0.4304526735057337 1103 58-59 0.4090075012849965 1103 60-61 0.49153137808006164 1103 62-63 0.2535194518117052 1103 64-65 0.22963015460616276 1103 66-67 0.06448973947156134 1103 68-69 0.1275447614261367 1103 70-71 0.22014054808524008 1103 72-73 0.18551221276028684 1103 74-75 -0.03720743319766484 1103 76-77 0.052395009807383985 1103 78-79 0.06393629094272413 1103 80-81 0.10413955416383658 1103 82-83 0.1048666406490959 1103 84-85 0.03724287912766755 1103 86-87 0.03091481443589217 1103 88-89 0.015363627818864245 1103 90-91 0.031419185667434846 1103 92-93 -0.04792467510901233 1103 94-95 -0.09333852927544584 1103 96-97 -0.08177665528829081 1103 98-99 -0.12492024658523704 1103 100-101 -0.24550082800344697 1104 1 0.14709178825899727 1104 2 0.2565113548767499 1104 3 0.23188500230845221 1104 4 0.2033847873757253 1104 5 0.2521171755160907 1104 6 0.23848158765669325 1104 7 0.2596011141572703 1104 8 0.2822788861023895 1104 9 0.291465632786597 1104 10-11 0.32185755569075525 1104 12-13 0.3820727506803365 1104 14-15 0.3737030117226823 1104 16-17 0.440318918480493 1104 18-19 0.4865144667659749 1104 20-21 0.4172664062470872 1104 22-23 0.5060833106227207 1104 24-25 0.4831928509514398 1104 26-27 0.4867193062181272 1104 28-29 0.5529615645044217 1104 30-31 0.5097908148615105 1104 32-33 0.459800628065409 1104 34-35 0.5862179571976682 1104 36-37 0.5813128335079156 1104 38-39 0.5049241436747494 1104 40-41 0.5384080976040906 1104 42-43 0.5206944402907325 1104 44-45 0.49263528222701325 1104 46-47 0.720229810633576 1104 48-49 0.7537110410099146 1104 50-51 0.606311032624582 1104 52-53 0.5544483813229135 1104 54-55 0.5189705074319946 1104 56-57 0.5509259708287928 1104 58-59 0.5728179203119836 1104 60-61 0.5122446203620683 1104 62-63 0.526445393020218 1104 64-65 0.6214636518650778 1104 66-67 0.5997172877436512 1104 68-69 0.6003521604281232 1104 70-71 0.4898502278760333 1104 72-73 0.46358180080018485 1104 74-75 0.5853734046428798 1104 76-77 0.5980485066987242 1104 78-79 0.4617226137984538 1104 80-81 0.4506611155653779 1104 82-83 0.4796707747752009 1104 84-85 0.4308529532291452 1104 86-87 0.3750742763805519 1104 88-89 0.27947478952063065 1104 90-91 0.444504537834721 1104 92-93 0.4078262374495871 1104 94-95 0.2602230133680301 1104 96-97 0.5222694756826165 1104 98-99 0.4918855493253389 1104 100-101 0.28299547047983964 1105 1 0.1014136919794808 1105 2 0.17156338261330006 1105 3 0.20217234815621055 1105 4 0.15511398278999167 1105 5 0.19910703032442711 1105 6 0.21798291980974938 1105 7 0.1656478740312579 1105 8 0.28636516796341027 1105 9 0.31029368523958567 1105 10-11 0.2693169185137023 1105 12-13 0.20294828332460213 1105 14-15 0.26340117274397556 1105 16-17 0.2755949388576653 1105 18-19 0.24885597526658643 1105 20-21 0.1891733811748395 1105 22-23 0.18963621055069524 1105 24-25 0.25054468689267395 1105 26-27 0.29252762371316265 1105 28-29 0.326816216625879 1105 30-31 0.36727126649177677 1105 32-33 0.3213089984994184 1105 34-35 0.213488041466114 1105 36-37 0.2980975280673519 1105 38-39 0.06416134502512705 1105 40-41 0.1129967356837085 1105 42-43 0.17054560364654492 1105 44-45 0.2133830236417893 1105 46-47 0.11518845747850293 1105 48-49 0.22516174266837652 1105 50-51 0.25643838992798607 1105 52-53 0.325518963738503 1105 54-55 0.24039040033903802 1105 56-57 0.26774364377003224 1105 58-59 0.26608382153305143 1105 60-61 0.18482374678209368 1105 62-63 0.24821004019492676 1105 64-65 0.24627229046540577 1105 66-67 0.28637536267400066 1105 68-69 0.20402425984925543 1105 70-71 0.023479281830390164 1105 72-73 0.18559812509917606 1105 74-75 0.17827372314462053 1105 76-77 0.2194942182073092 1105 78-79 0.14077179497995118 1105 80-81 0.19683540631320895 1105 82-83 0.17867100909528233 1105 84-85 0.020046892149707674 1105 86-87 0.09460420426203697 1105 88-89 0.05526540283174697 1105 90-91 0.142780983459442 1105 92-93 0.14668995862604461 1105 94-95 0.22969935137908593 1105 96-97 0.15716038666701948 1105 98-99 -0.03800479755516406 1105 100-101 -0.20602294534284127 1106 1 0.026397857569406824 1106 2 0.02075815007971471 1106 3 0.12904735343435192 1106 4 0.13372823967056746 1106 5 0.17555851791446742 1106 6 0.046751720532640206 1106 7 0.11637372338901741 1106 8 0.15474828468201451 1106 9 0.22872324956107093 1106 10-11 0.1968050321498751 1106 12-13 0.23945842156955166 1106 14-15 0.18091234128120703 1106 16-17 0.20556968825173527 1106 18-19 0.16964338881070518 1106 20-21 0.2546974368106234 1106 22-23 0.2448613547493821 1106 24-25 0.3000432639070283 1106 26-27 0.2584287494341524 1106 28-29 0.27888081257396635 1106 30-31 0.3252342857548669 1106 32-33 0.30323053583062176 1106 34-35 0.21770590605868279 1106 36-37 0.2627188112034915 1106 38-39 0.24751832441084076 1106 40-41 0.3072089421137463 1106 42-43 0.4077188869853643 1106 44-45 0.3701202484397399 1106 46-47 0.2706730748769317 1106 48-49 0.2736011032115613 1106 50-51 0.21584930764817045 1106 52-53 0.18318788522515916 1106 54-55 0.14017589686390863 1106 56-57 0.14538482337024305 1106 58-59 0.10324971548050854 1106 60-61 0.09395661226138685 1106 62-63 0.18171100714956623 1106 64-65 0.20306715251602014 1106 66-67 0.07786405057144208 1106 68-69 0.06137797101273179 1106 70-71 0.010773540062174902 1106 72-73 0.06844375860225682 1106 74-75 0.07284610962245353 1106 76-77 0.1210090434376454 1106 78-79 0.010599643273632608 1106 80-81 0.2189660000824709 1106 82-83 0.13118741354411867 1106 84-85 0.14915370474630052 1106 86-87 0.15654018257523106 1106 88-89 0.03273641530504179 1106 90-91 -0.08685976544481733 1106 92-93 -0.05208108002680589 1106 94-95 0.07743334173487426 1106 96-97 0.14476901072012183 1106 98-99 0.1569670383074495 1106 100-101 0.1090142549645563 1107 1 0.14331855908225322 1107 2 0.17276396463103083 1107 3 0.17700035428185146 1107 4 0.10081546551696619 1107 5 0.09842614595203969 1107 6 0.1965186297609165 1107 7 0.18473745539578346 1107 8 0.19757296383719591 1107 9 0.2834803686522136 1107 10-11 0.16157109170897854 1107 12-13 0.15114870579201067 1107 14-15 0.1279801167390886 1107 16-17 0.1618579977780925 1107 18-19 0.09505785959670021 1107 20-21 0.24907879647611963 1107 22-23 0.2642409826051093 1107 24-25 0.26807040244855784 1107 26-27 0.31044771491594503 1107 28-29 0.31796410612978576 1107 30-31 0.35359875919268546 1107 32-33 0.30906903482780024 1107 34-35 0.40713152969961897 1107 36-37 0.42857477441985026 1107 38-39 0.515050463011903 1107 40-41 0.5240372082799212 1107 42-43 0.3974998563442398 1107 44-45 0.43024421896000575 1107 46-47 0.5451343467396796 1107 48-49 0.5939721109841329 1107 50-51 0.3882708593713744 1107 52-53 0.5338317396275869 1107 54-55 0.4127099891628774 1107 56-57 0.3865973770428184 1107 58-59 0.4875224744374762 1107 60-61 0.3905809902981474 1107 62-63 0.3821485055936549 1107 64-65 0.5152282995802224 1107 66-67 0.47619707542616396 1107 68-69 0.4788389701154401 1107 70-71 0.4579115349944658 1107 72-73 0.5201203128203531 1107 74-75 0.4026573748285571 1107 76-77 0.42295345992058486 1107 78-79 0.4767610273793004 1107 80-81 0.4774756803579159 1107 82-83 0.5338557806315052 1107 84-85 0.5254417382654779 1107 86-87 0.41639918620012395 1107 88-89 0.509124406719593 1107 90-91 0.6026272833240292 1107 92-93 0.47757494466880246 1107 94-95 0.5946327834427017 1107 96-97 0.7414583857424439 1107 98-99 0.4844974559225541 1107 100-101 0.3794349981490761 1108 1 0.06616402741265404 1108 2 0.20326076473035215 1108 3 0.13148650012699648 1108 4 0.18126513529626465 1108 5 0.23207755432218846 1108 6 0.24457690427452405 1108 7 0.18875696351820892 1108 8 0.2906165954346349 1108 9 0.22391738637614367 1108 10-11 0.28025775592337254 1108 12-13 0.2946548108842677 1108 14-15 0.3505776978367763 1108 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-1.3818093014705468 2316 60-61 -1.3272590426256627 2316 62-63 -1.4959744877944559 2316 64-65 -1.5631008483190172 2316 66-67 -1.612782282571267 2316 68-69 -1.6182411209951155 2316 70-71 -1.6678711717667234 2316 72-73 -1.9634455052213298 2316 74-75 -1.6646695120967827 2316 76-77 -1.5077757349122685 2316 78-79 -1.1401995177377806 2316 80-81 -1.341688284469992 2316 82-83 -1.3214916735228108 2316 84-85 -1.3443655235196132 2316 86-87 -1.4038529003834022 2316 88-89 -1.6899181203233589 2316 90-91 -1.8200269977376138 2316 92-93 -1.8731653432075852 2316 94-95 -1.8075509569511858 2316 96-97 -1.7548673120289315 2316 98-99 -2.0665602683167243 2316 100-101 -1.8490125380219204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8414.0 3 2269.0 4 2262.0 5 2547.0 6 2907.0 7 3387.0 8 4156.0 9 5373.0 10 6444.0 11 7960.0 12 10170.0 13 16059.0 14 13157.0 15 11962.0 16 13726.0 17 17641.0 18 15541.0 19 12867.0 20 13511.0 21 14557.0 22 15956.0 23 17519.0 24 20233.0 25 23260.0 26 27053.0 27 31680.0 28 37300.0 29 44315.0 30 54438.0 31 66661.0 32 83948.0 33 109895.0 34 153366.0 35 228838.0 36 380176.0 37 645639.0 38 646532.0 39 109443.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.987848791464884 18.497490102228767 13.67976171477275 23.834899391533597 2 15.410770404738045 19.928959918160047 40.218958013935584 24.441311663166324 3 20.36540895320739 23.12362870869794 29.090939417505684 27.420022920588988 4 13.003333225596919 15.7335476940411 36.959011160449755 34.30410791991223 5 14.244232621230598 36.84967437017503 34.159487889364755 14.746605119229624 6 33.10835054106671 35.066715062512266 17.253975724504915 14.570958671916113 7 29.540852641199354 31.68206050614347 20.4613531780645 18.31573367459268 8 25.700447784990953 35.11515240355741 19.616254758431964 19.568145053019677 9 25.661271453025652 17.752656618202835 20.6244650638033 35.96160686496821 10-11 25.463079310467602 25.757392344144435 27.373387300564538 21.406141044823425 12-13 26.458755712705432 23.11577767044066 27.579855428366283 22.845611188487624 14-15 22.841478541132552 25.009039681858773 26.03409271292228 26.115389064086397 16-17 22.67201773717312 28.131259388268298 26.480365678097613 22.716357196460972 18-19 22.41409377298183 27.504079193144804 27.979304760280314 22.102522273593056 20-21 23.297193026823333 26.357232878311233 27.67498596582888 22.670588129036563 22-23 22.7653292115661 26.330185378774217 27.76646353135812 23.138021878301558 24-25 23.07462980616129 26.41097392603264 27.800261676092532 22.714134591713545 26-27 22.850191960021444 26.302657835822323 28.243314756407617 22.60383544774862 28-29 22.394055746400195 27.2124610990219 27.911158626349437 22.482324528228464 30-31 22.75795389919022 26.404645447467733 27.73540515648977 23.10199549685228 32-33 22.601730464014558 26.55772940998476 27.92108950689714 22.919450619103543 34-35 23.371567177811496 26.52989506778356 27.52946511335107 22.569072641053875 36-37 23.060848111778785 26.259378293307652 27.96552975579797 22.714243839115596 38-39 22.681954868068352 26.729360177990586 27.975705973618904 22.61297898032216 40-41 22.908709904020082 26.766008919247536 27.673664083530923 22.651617093201462 42-43 22.611840357374184 26.739461234834007 27.21712543560997 23.431572972181844 44-45 22.897423389775625 26.531454296257838 27.4295727469141 23.14154956705244 46-47 23.201978917045707 26.907964795681245 27.171955316307116 22.718100970965928 48-49 23.22723857076085 26.680597532607177 27.262839569219054 22.82932432741292 50-51 22.461978816551138 26.99227930650715 27.731897771339554 22.813844105602158 52-53 22.980975806009816 27.181958611622203 26.906754895621827 22.930310686746154 54-55 22.678046972108948 26.531539350589878 27.35680048847504 23.433613188826133 56-57 22.967896830075322 26.719690590683538 27.23108240939111 23.08133016985003 58-59 23.450064452765332 26.53685665816597 27.052757797110505 22.960321091958193 60-61 23.052343330191942 26.584275823988314 27.835354497854333 22.52802634796542 62-63 22.652981912779317 26.77377525685514 27.590032300726424 22.983210529639116 64-65 22.498284449672568 27.13073269920056 27.21439467550407 23.1565881756228 66-67 22.70144812419503 26.779260741052763 26.971869338152676 23.547421796599526 68-69 22.43346923346358 26.685186920707586 27.414181956085777 23.467161889743057 70-71 22.964379110345963 26.580375396691743 27.151070511438945 23.30417498152335 72-73 22.936333059501532 26.207186444558133 27.73983024289602 23.116650253044313 74-75 22.522354314156225 27.12733517799431 27.154434489029665 23.1958760188198 76-77 22.716505762205653 26.61455153187792 27.043009814765064 23.62593289115136 78-79 22.78142401297018 26.65965905840627 27.29972681140918 23.25919011721437 80-81 22.869468816628157 26.65577941685364 27.24252578614246 23.23222598037574 82-83 22.866007518814044 26.532994984956964 27.440164966638775 23.16083252959022 84-85 22.686222983527376 26.65685187720931 27.2816202077677 23.37530493149562 86-87 22.650788697404256 26.722302006678404 27.318432314222335 23.308476981695005 88-89 22.60598312755378 26.654229294025 26.97855582495055 23.761231753470664 90-91 22.824673426852236 26.308992768410285 27.178144528847405 23.688189275890075 92-93 23.08345468235639 26.492156737859062 27.21791259903869 23.206475980745857 94-95 22.92949374964884 26.4333441865156 27.2253647594031 23.41179730443246 96-97 22.498960037522405 26.357360421680355 27.688076167131044 23.455603373666197 98-99 22.748190384324452 26.526488885802312 26.914437542499776 23.81088318737346 100-101 22.83718933395391 25.699415646820896 27.492476433193463 23.97091858603173 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2266.0 1 1221.0 2 214.0 3 248.0 4 225.0 5 236.0 6 362.0 7 709.5 8 1140.5 9 1387.5 10 1477.5 11 1558.0 12 1789.0 13 2089.5 14 2321.0 15 2609.5 16 2923.0 17 2962.5 18 2934.5 19 3078.0 20 3280.0 21 3526.5 22 4003.5 23 4990.5 24 6228.0 25 8035.5 26 11001.5 27 14709.0 28 18534.0 29 22943.5 30 27994.5 31 33933.5 32 40144.5 33 44971.0 34 49100.0 35 54158.5 36 60460.0 37 66456.0 38 70403.5 39 74215.5 40 80024.0 41 86957.0 42 92846.5 43 98681.0 44 103760.5 45 106527.0 46 108840.5 47 119962.5 48 143977.0 49 143075.0 50 120409.5 51 110224.0 52 106681.5 53 104127.0 54 99644.0 55 91336.5 56 82135.0 57 74459.0 58 67581.5 59 59287.5 60 49769.5 61 40513.5 62 32631.0 63 26223.0 64 20171.5 65 15072.5 66 11290.5 67 8928.5 68 6629.5 69 4587.5 70 3490.0 71 2717.5 72 2173.5 73 1838.0 74 1393.0 75 1085.5 76 840.5 77 503.0 78 310.0 79 203.5 80 146.5 81 108.0 82 75.0 83 64.0 84 45.5 85 32.0 86 24.5 87 13.0 88 11.0 89 10.0 90 10.5 91 7.0 92 5.0 93 6.0 94 4.0 95 4.5 96 3.5 97 3.0 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.394073008671411 2 0.11731255674734624 3 0.11904794959863835 4 0.13185514884117408 5 0.1334517102643628 6 0.07486484760474137 7 0.08479129471413221 8 0.0817370032958581 9 0.08135521686857383 10-11 0.07929009937553623 12-13 0.08369799721781818 14-15 0.07701673474034358 16-17 0.07866535794907108 18-19 0.08881740612912989 20-21 0.08690847399270857 22-23 0.09770261752774546 24-25 0.11129074355336263 26-27 0.11276582747696093 28-29 0.10589367178584419 30-31 0.09582839324834999 32-33 0.09424918575367418 34-35 0.3456034863348225 36-37 0.5390824353253793 38-39 0.5196807432479335 40-41 0.49056085120325205 42-43 0.5189692321789038 44-45 0.5295030667862468 46-47 0.5268652696522828 48-49 0.5335812399867832 50-51 0.5318111392784654 52-53 0.5315508303507714 54-55 0.5251125228724778 56-57 0.49666943403980024 58-59 0.485077009793169 60-61 0.43410852175071984 62-63 0.5099625432806977 64-65 0.5377461828298843 66-67 0.5482279556516886 68-69 0.5629787948876714 70-71 0.5584320656172862 72-73 0.5444274453073589 74-75 0.548575034221947 76-77 0.5241580568042671 78-79 0.49660001832574857 80-81 0.5045134097276406 82-83 0.5176850414689476 84-85 0.5126176943431746 86-87 0.5132771436266657 88-89 0.5240539332331896 90-91 0.5428135299556572 92-93 0.5495815620756964 94-95 0.5439762431660229 96-97 0.545052186733824 98-99 0.5461454842301381 100-101 0.5217111528839452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2881192.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.31778910547216 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0825062952705 38.01750434465505 2 14.010360542796246 14.379614552536923 3 4.922132084178911 7.577788087355144 4 2.3032888598681778 4.727987678387943 5 1.2310326959683666 3.158693813682272 6 0.774255794281179 2.383985733876682 7 0.4986139867535425 1.7911437192079886 8 0.3636595081501424 1.4929761556358991 9 0.2603382867041408 1.2023986763846737 >10 1.3879307588613021 13.142184060335829 >50 0.10833739194591359 3.821798059409404 >100 0.05435440912437041 4.874538915001439 >500 0.0014250448519321968 0.5245869511067116 >1k 0.0014929041305436642 1.7131260270907742 >5k 2.0357783598322696E-4 0.695398089908844 >10k+ 6.785927866107565E-5 0.49627513542445467 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14251 0.4946216704752755 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7776 0.269888296232948 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6491 0.22528869995474096 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5702 0.19790420076135157 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4160 0.14438468522750306 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4100 0.14230221380595254 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3511 0.12185928601773155 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3316 0.11509125389769234 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3052 0.10592837964287004 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3049 0.10582425607179251 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2912 0.10106927965925214 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.470785702584208E-5 0.0 8 0.0 0.0 0.0 1.3883142810336833E-4 0.0 9 0.0 0.0 0.0 1.7353928512921042E-4 0.0 10-11 0.0 0.0 0.0 1.9089321364213147E-4 0.0 12-13 0.0 0.0 0.0 2.4295499918089458E-4 0.0 14-15 0.0 0.0 0.0 2.4295499918089458E-4 0.0 16-17 0.0 0.0 0.0 2.6030892769381565E-4 0.0 18-19 0.0 0.0 0.0 2.7766285620673667E-4 0.0 20-21 0.0 0.0 0.0 2.7766285620673667E-4 0.0 22-23 0.0 0.0 0.0 3.6443249877134187E-4 0.0 24-25 0.0 0.0 0.0 6.594492834909997E-4 0.0 26-27 0.0 0.0 0.0 9.891739252364993E-4 0.0 28-29 0.0 0.0 0.0 0.0024295499918089458 0.0 30-31 0.0 0.0 0.0 0.007132464618810548 0.0 32-33 0.0 0.0 0.0 0.01803073172492496 0.0 34-35 0.0 0.0 0.0 0.03543672202338477 0.0 36-37 0.0 0.0 0.0 0.06492104656683761 0.0 38-39 0.0 0.0 0.0 0.1092776878458638 0.0 40-41 0.0 0.0 0.0 0.16014205231723538 0.0 42-43 1.735392851292104E-5 0.0 0.0 0.2154143146308889 0.0 44-45 3.470785702584208E-5 0.0 0.0 0.28392762439990116 0.0 46-47 3.470785702584208E-5 0.0 0.0 0.3706278512504547 0.0 48-49 3.470785702584208E-5 0.0 0.0 0.4582304823836801 0.0 50-51 3.470785702584208E-5 0.0 0.0 0.5525664377799189 0.0 52-53 3.470785702584208E-5 0.0 0.0 0.6527506670850121 0.0 54-55 3.470785702584208E-5 0.0 0.0 0.7696293756195353 0.0 56-57 3.470785702584208E-5 0.0 0.0 0.9097970562183986 0.0 58-59 3.470785702584208E-5 0.0 0.0 1.0600473692832688 0.0 60-61 3.470785702584208E-5 0.0 0.0 1.2260724033663846 0.0 62-63 3.470785702584208E-5 1.735392851292104E-5 0.0 1.3961235488644976 0.0 64-65 3.470785702584208E-5 3.470785702584208E-5 0.0 1.5793289721754051 0.0 66-67 3.470785702584208E-5 3.470785702584208E-5 0.0 1.7951597810906041 0.0 68-69 3.470785702584208E-5 3.470785702584208E-5 0.0 2.043980408108866 0.0 70-71 3.470785702584208E-5 3.470785702584208E-5 0.0 2.3146322771963828 0.0 72-73 3.470785702584208E-5 3.470785702584208E-5 1.735392851292104E-5 2.5918265773332703 0.0 74-75 3.470785702584208E-5 3.470785702584208E-5 3.470785702584208E-5 2.887572227050471 0.0 76-77 3.470785702584208E-5 3.470785702584208E-5 3.470785702584208E-5 3.2101991120341857 0.0 78-79 3.470785702584208E-5 3.470785702584208E-5 3.470785702584208E-5 3.5848357207711254 0.0 80-81 3.470785702584208E-5 3.470785702584208E-5 3.470785702584208E-5 3.9965923825972025 0.0 82-83 3.470785702584208E-5 3.470785702584208E-5 3.470785702584208E-5 4.407134269427376 0.0 84-85 3.470785702584208E-5 3.470785702584208E-5 3.470785702584208E-5 4.831246928354653 0.0 86-87 3.470785702584208E-5 3.470785702584208E-5 3.470785702584208E-5 5.293850600723589 0.0 88-89 3.470785702584208E-5 5.206178553876312E-5 3.470785702584208E-5 5.799353184376466 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8475 0.0 26.562643 1 TACATGG 8685 0.0 25.193907 2 ACATGGG 8635 0.0 24.35295 3 GAGTACT 5575 0.0 19.31874 12-13 CATGGGG 5080 0.0 18.926191 4 TAGCCCT 1780 0.0 17.827307 4 GTGTAGC 1750 0.0 17.64038 1 AGTACTT 6190 0.0 17.09343 12-13 AGAGTAC 10275 0.0 17.069113 10-11 GTACTTT 6200 0.0 17.028566 14-15 CATGGGA 3955 0.0 16.885098 4 GTATTAG 1030 0.0 16.599611 1 TACCTGG 2225 0.0 15.964476 2 GTAGCCC 1650 0.0 15.787367 3 AGGACCG 395 6.7993824E-9 15.586985 5 GTATAGC 1015 0.0 15.441183 1 GAGTACA 7210 0.0 15.282183 1 CGATCGT 285 1.8189894E-12 14.953983 24-25 TACTTTT 7340 0.0 14.480555 14-15 AGTACAT 6455 0.0 14.307434 2 >>END_MODULE