FastQCFastQC Report
Fri 27 May 2016
SRR939104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881192
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT157180.5455380967321858No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69070.23972716847749123No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56680.19672413362247293No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51740.17957845225170693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44880.15576886233197926No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43880.15229807662939507No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG35620.1236293867260495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35530.12331701601281692No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35400.12286581387148097No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35370.12276169030040343No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33180.11516066961174402No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG85850.024.319171
GTAAGCG4850.023.96914594-95
TACATGG86250.023.8208372
ACATGGG86750.022.6443083
GTGTAGC18050.019.80611
CATGGGG51750.019.071344
GAGTACT60050.018.33394612-13
GTACTTT63300.017.13024114-15
AGAGTAC106400.016.94802910-11
GCACCGT5350.016.8510916
AGTACTT64550.016.61472512-13
GAGTACA73450.016.2241951
AGTACAT66550.015.9708562
TAGACTG11100.015.8163765
GTAGCCC15950.015.7667943
CATGGGA37050.015.4962054
GTATAGC9350.015.2941241
ATGGGGA33750.015.183725
TAGTACC7850.014.506744
TCTATAC9700.014.1858213
Produced by FastQC (version 0.11.5)