FastQCFastQC Report
Fri 27 May 2016
SRR939103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724216
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23400.13571385487665116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.1142548265414542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC905.823822E-736.843356
GTACATG78000.028.9709361
TACATGG78750.028.0960662
ACATGGG78500.026.9784583
TACCTGG17600.025.8426932
CATGGGG39200.025.5020794
GTATCAA69300.024.5244881
GTACCTG20000.024.2114241
TATCGCT1601.9053241E-623.6850137
GGTATCA52800.023.1972221
TATATCG1100.002921109621.5355785
ATGGGGA24100.021.2317585
ATCAACG79250.020.8045923
TCAACGC79500.020.7987654
TATCAAC80850.020.6272772
CAACGCA80850.020.6272775
AACGCAG83300.020.1877046
GCGTTCG958.164554E-620.02666334-35
GAGTACT32400.019.88169112-13
CATGGGA37200.019.3588644
Produced by FastQC (version 0.11.5)