##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939103_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1724216 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09111445433751 33.0 31.0 34.0 30.0 34.0 2 32.182232968491185 34.0 31.0 34.0 30.0 34.0 3 32.25272819646727 34.0 31.0 34.0 30.0 34.0 4 35.683436413999175 37.0 35.0 37.0 35.0 37.0 5 35.72723429083131 37.0 35.0 37.0 35.0 37.0 6 35.807223688911364 37.0 36.0 37.0 35.0 37.0 7 35.727175713483696 37.0 36.0 37.0 35.0 37.0 8 35.71241422188403 37.0 36.0 37.0 35.0 37.0 9 37.40186554352819 39.0 38.0 39.0 35.0 39.0 10-11 37.30889894305586 39.0 37.5 39.0 34.5 39.0 12-13 37.21236318419502 39.0 37.0 39.0 34.0 39.0 14-15 38.553052807768864 40.0 38.0 41.0 34.0 41.0 16-17 38.48281566810655 40.0 38.0 41.0 34.0 41.0 18-19 38.383575491701734 40.0 38.0 41.0 34.0 41.0 20-21 38.27248790174781 40.0 38.0 41.0 34.0 41.0 22-23 38.198539510130985 40.0 38.0 41.0 33.5 41.0 24-25 38.244702519869904 40.0 38.0 41.0 34.0 41.0 26-27 38.1719941121066 40.0 38.0 41.0 34.0 41.0 28-29 38.05729763556306 40.0 38.0 41.0 33.0 41.0 30-31 37.926856611932614 40.0 38.0 41.0 33.0 41.0 32-33 37.76680212919959 40.0 38.0 41.0 33.0 41.0 34-35 37.482428825622776 40.0 37.5 41.0 31.5 41.0 36-37 37.24497800739582 40.0 37.0 41.0 31.0 41.0 38-39 37.03249679854496 40.0 37.0 41.0 30.5 41.0 40-41 36.90101182218469 40.0 37.0 41.0 30.5 41.0 42-43 36.733395641845334 40.0 36.5 41.0 30.0 41.0 44-45 36.51934821391288 40.0 36.0 41.0 30.0 41.0 46-47 36.22200814747109 39.0 36.0 41.0 29.5 41.0 48-49 36.28648469797288 40.0 36.0 41.0 30.0 41.0 50-51 35.307892978605935 38.5 34.5 40.0 27.5 40.5 52-53 35.4716555234379 38.5 35.0 40.0 28.0 40.5 54-55 35.97884748778576 39.0 35.0 41.0 29.0 41.0 56-57 35.86848573496592 39.0 35.0 41.0 28.0 41.0 58-59 35.68566061328743 39.0 35.0 41.0 28.0 41.0 60-61 35.39785792499316 38.5 35.0 41.0 28.0 41.0 62-63 35.024227242990435 38.0 35.0 40.0 27.0 41.0 64-65 34.65318382383646 37.5 34.0 40.0 26.0 41.0 66-67 34.269128113879006 37.0 34.0 40.0 26.0 41.0 68-69 33.87873068107476 36.0 34.0 39.0 26.0 41.0 70-71 33.47004406640467 36.0 34.0 39.0 25.0 40.5 72-73 33.019874250093956 35.0 33.0 38.5 24.5 40.0 74-75 32.56294165000209 35.0 33.0 37.0 23.0 39.5 76-77 32.129047346736144 35.0 32.5 37.0 22.0 39.0 78-79 31.712150623819753 35.0 32.0 36.0 20.5 39.0 80-81 31.300394208150255 35.0 32.0 36.0 19.5 37.5 82-83 30.89845036816733 35.0 31.0 35.5 18.0 37.0 84-85 30.53891043813536 34.5 31.0 35.0 16.0 36.5 86-87 30.166857864675887 34.0 31.0 35.0 9.0 36.0 88-89 29.792681137398098 34.0 30.5 35.0 4.5 36.0 90-91 29.44625731346885 34.0 30.0 35.0 2.0 35.5 92-93 29.05533442445726 34.0 30.0 35.0 2.0 35.0 94-95 28.6245783590919 34.0 29.0 35.0 2.0 35.0 96-97 28.07589652340542 34.0 29.0 35.0 2.0 35.0 98-99 27.39645815837459 34.0 28.0 35.0 2.0 35.0 100-101 24.841349343701715 31.5 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2946132930313681 1101 2 0.36165832491681726 1101 3 0.27187497949658024 1101 4 0.33040570696309146 1101 5 0.4158373329430134 1101 6 0.512164992596901 1101 7 0.25778292386974755 1101 8 0.44412825956177926 1101 9 0.4382164403297679 1101 10-11 0.5445547035313183 1101 12-13 0.5034921417347036 1101 14-15 0.5709634017293084 1101 16-17 0.5461252663266762 1101 18-19 0.5832251076738046 1101 20-21 0.6506799514407504 1101 22-23 0.6023736857889688 1101 24-25 0.7541106658903445 1101 26-27 0.7733755948542225 1101 28-29 0.6847492118773957 1101 30-31 0.6554605809319938 1101 32-33 0.5751319295642716 1101 34-35 0.5735955590826904 1101 36-37 0.39807556134928745 1101 38-39 0.5631265062115816 1101 40-41 0.5732224727768909 1101 42-43 0.531166877878583 1101 44-45 0.48784009117909477 1101 46-47 0.558802767274102 1101 48-49 0.6490226064929701 1101 50-51 0.6202815094160528 1101 52-53 0.6465020567646249 1101 54-55 0.5985115376114507 1101 56-57 0.6468031476347633 1101 58-59 0.5427811782194567 1101 60-61 0.5819068814770603 1101 62-63 0.6220950708509321 1101 64-65 0.5755621264612714 1101 66-67 0.3191969361005036 1101 68-69 0.24704909075620662 1101 70-71 0.4047649104137747 1101 72-73 0.3332614311105857 1101 74-75 0.1764471570630448 1101 76-77 0.17556680786540824 1101 78-79 0.30534754365870853 1101 80-81 0.09745838769836368 1101 82-83 0.03421487584635585 1101 84-85 0.02602815543000858 1101 86-87 -0.059828944354865854 1101 88-89 -0.13647034571351213 1101 90-91 -0.2126122432133073 1101 92-93 -0.06449860760481485 1101 94-95 -0.1581702976758983 1101 96-97 0.1115696083928448 1101 98-99 -0.4362695018364171 1101 100-101 -0.6131371933769678 1102 1 0.13555903725400498 1102 2 0.1243702272673417 1102 3 0.21180221340919303 1102 4 0.2495270873517086 1102 5 0.2510108620624294 1102 6 0.22756461058837374 1102 7 0.19852996276638635 1102 8 0.26257705062926817 1102 9 0.2952238252906696 1102 10-11 0.1154219416884743 1102 12-13 0.23979947323589812 1102 14-15 0.3351835034728836 1102 16-17 0.3958782054909662 1102 18-19 0.3913571611979805 1102 20-21 0.4766131008440553 1102 22-23 0.47955427302185427 1102 24-25 0.38895470593944736 1102 26-27 0.4096270936511459 1102 28-29 0.40614894269839397 1102 30-31 0.3951986910733112 1102 32-33 0.4818720607867988 1102 34-35 0.2637455417553056 1102 36-37 0.30080890130911797 1102 38-39 0.35504021132714314 1102 40-41 0.18228429529604284 1102 42-43 0.24816737665670985 1102 44-45 0.16733775802575224 1102 46-47 0.08210900361255113 1102 48-49 0.16074434023499862 1102 50-51 0.20272295223700354 1102 52-53 0.06073863520040135 1102 54-55 -0.1291238152807921 1102 56-57 -0.12670165970882152 1102 58-59 -0.18586717782753226 1102 60-61 -0.08594964414374573 1102 62-63 0.03677244557658099 1102 64-65 -0.08062169253152263 1102 66-67 -0.19260001732329357 1102 68-69 -0.17202102383739515 1102 70-71 -0.2876794964208287 1102 72-73 -0.28291603898287576 1102 74-75 -0.3162010145166292 1102 76-77 -0.39950758350438775 1102 78-79 -0.39083747526710155 1102 80-81 -0.30562575357494737 1102 82-83 -0.36295578955232344 1102 84-85 -0.5226447992438672 1102 86-87 -0.5937319772303447 1102 88-89 -0.6199318659211919 1102 90-91 -0.739209340629067 1102 92-93 -0.7869773826702087 1102 94-95 -0.9783028007272172 1102 96-97 -0.9319319931595444 1102 98-99 -1.1897085635962554 1102 100-101 -1.0908603025646073 1103 1 0.30935326542149255 1103 2 0.2448777127248789 1103 3 0.35076293806100267 1103 4 0.29871662601217963 1103 5 0.28456586003563444 1103 6 0.32020195800151185 1103 7 0.27626908606220724 1103 8 0.29578398722736665 1103 9 0.2798445581180715 1103 10-11 0.31048703857268123 1103 12-13 0.36157071224887005 1103 14-15 0.39978908237505806 1103 16-17 0.472577206802832 1103 18-19 0.4720075570643445 1103 20-21 0.5025787359833771 1103 22-23 0.5046181127802143 1103 24-25 0.4222242850331952 1103 26-27 0.41519928260657224 1103 28-29 0.4257504851148468 1103 30-31 0.4292715051709095 1103 32-33 0.5372379784801211 1103 34-35 0.47846776825345927 1103 36-37 0.30383220346230644 1103 38-39 0.46847358451221766 1103 40-41 0.3969180098720386 1103 42-43 0.47588268297637626 1103 44-45 0.39533100033290935 1103 46-47 0.19410630292993858 1103 48-49 0.4601047026293159 1103 50-51 0.6098348210443802 1103 52-53 0.5643090436551219 1103 54-55 0.5071000950818672 1103 56-57 0.42965734464750227 1103 58-59 0.46365319017682083 1103 60-61 0.5686494695397641 1103 62-63 0.4246130881987895 1103 64-65 0.26612996268418243 1103 66-67 0.2175351536372787 1103 68-69 0.1810288746518296 1103 70-71 0.1192448724934394 1103 72-73 -0.13889687935868267 1103 74-75 -0.10814433438930138 1103 76-77 -0.009185827701507776 1103 78-79 -0.013423280520346026 1103 80-81 0.11053934553275013 1103 82-83 -0.05072485296790674 1103 84-85 -0.0949601086422085 1103 86-87 -0.11545566780976912 1103 88-89 0.05737183858068562 1103 90-91 -0.2003175441021554 1103 92-93 -0.23836039214397786 1103 94-95 -0.1318449024706041 1103 96-97 -0.0747226757190198 1103 98-99 -0.2608507693304567 1103 100-101 -0.3213538997746461 1104 1 0.20360850095929095 1104 2 0.2574208987481583 1104 3 0.350282365631287 1104 4 0.1891510241914034 1104 5 0.2148754894954621 1104 6 0.23545583450995622 1104 7 0.23021436466322598 1104 8 0.23318021796762878 1104 9 0.2645145432178708 1104 10-11 0.3072609812314653 1104 12-13 0.3392273690366494 1104 14-15 0.4240100965711875 1104 16-17 0.4008839053439246 1104 18-19 0.423438222006304 1104 20-21 0.5454986842420197 1104 22-23 0.4395525355202139 1104 24-25 0.5409907612174365 1104 26-27 0.3449763526702654 1104 28-29 0.41092601276208995 1104 30-31 0.2823776240777889 1104 32-33 0.37484174773985046 1104 34-35 0.22905886415508547 1104 36-37 0.4536353079438342 1104 38-39 0.4655876938567758 1104 40-41 0.32297043893234445 1104 42-43 0.22930277041272262 1104 44-45 0.19482545778546978 1104 46-47 0.16003764738657367 1104 48-49 0.21211336439474593 1104 50-51 0.2546725562137695 1104 52-53 0.08034231531363645 1104 54-55 0.011515712457786265 1104 56-57 0.12237343850240023 1104 58-59 0.21376985384161173 1104 60-61 0.2889423976749512 1104 62-63 0.16729356893495861 1104 64-65 0.24436993779593053 1104 66-67 0.28446898691876044 1104 68-69 0.25681154457143407 1104 70-71 0.2723935634043215 1104 72-73 0.31529073218815995 1104 74-75 0.2751801297765226 1104 76-77 0.10399348230454564 1104 78-79 0.1662972997688179 1104 80-81 0.08836588099031673 1104 82-83 0.022559167794131696 1104 84-85 -0.12506056219778827 1104 86-87 0.10280403778844871 1104 88-89 -0.04733523328413014 1104 90-91 -0.1060156430984378 1104 92-93 -0.2615907885548303 1104 94-95 -0.29565133084321005 1104 96-97 -0.16565647625280988 1104 98-99 -0.3809562169095848 1104 100-101 -0.34650167375005125 1105 1 0.1551810768187707 1105 2 0.18442568570863926 1105 3 0.23041054539945804 1105 4 0.2669964963597806 1105 5 0.2028289649870203 1105 6 0.2811258111685788 1105 7 0.2426169440832524 1105 8 0.22180228545980896 1105 9 0.20976121014827953 1105 10-11 0.26578849636379687 1105 12-13 0.32021008059260936 1105 14-15 0.4166239352387535 1105 16-17 0.35100852456297105 1105 18-19 0.3976638025736108 1105 20-21 0.3586015049881439 1105 22-23 0.45052496104539586 1105 24-25 0.28047725091162334 1105 26-27 0.44330487266868346 1105 28-29 0.453975029654913 1105 30-31 0.3772899950078781 1105 32-33 0.3542321026079165 1105 34-35 0.1928915974699592 1105 36-37 0.2320623364677843 1105 38-39 0.29204652995283453 1105 40-41 0.47130687126212933 1105 42-43 0.44140863740642544 1105 44-45 0.258513791052863 1105 46-47 0.403674154883511 1105 48-49 0.467970467690229 1105 50-51 0.47906033957413996 1105 52-53 0.32878556411689175 1105 54-55 0.3606651368356779 1105 56-57 0.3109559443377421 1105 58-59 0.2876057520890285 1105 60-61 0.34000858432684566 1105 62-63 0.27769432851876985 1105 64-65 0.2631656809616558 1105 66-67 0.21561192318493028 1105 68-69 0.09348915662982193 1105 70-71 -0.039453174737076324 1105 72-73 0.044688999387588524 1105 74-75 0.14603703526372414 1105 76-77 0.1839970428536617 1105 78-79 0.08039707426737053 1105 80-81 0.012763554764894991 1105 82-83 0.0824614505017287 1105 84-85 0.15806045691049064 1105 86-87 0.10491069601529546 1105 88-89 0.12236449374043801 1105 90-91 0.11502918025876596 1105 92-93 0.2009265196700376 1105 94-95 0.056198176614163486 1105 96-97 0.1618626122466189 1105 98-99 -0.2024896267287879 1105 100-101 -0.42534805583653323 1106 1 0.22487044117863064 1106 2 0.1891815874792684 1106 3 0.16159212566775238 1106 4 0.296197472676063 1106 5 0.26487332836979505 1106 6 0.24831767571043883 1106 7 0.27600979643649026 1106 8 0.32210307363979496 1106 9 0.3614331709423624 1106 10-11 0.3567010532165966 1106 12-13 0.32157608041635655 1106 14-15 0.32707182224968534 1106 16-17 0.38326719473436555 1106 18-19 0.3882849589030002 1106 20-21 0.444324603604521 1106 22-23 0.3572028418106612 1106 24-25 0.5020523847336236 1106 26-27 0.37995869037954577 1106 28-29 0.3527868205515716 1106 30-31 0.3539423183570918 1106 32-33 0.379287183245971 1106 34-35 0.44556573849938275 1106 36-37 0.43425904810579397 1106 38-39 0.5326533174201202 1106 40-41 0.564501969755888 1106 42-43 0.41119685953754015 1106 44-45 0.41956248808445196 1106 46-47 0.3793003498938745 1106 48-49 0.3402044617357731 1106 50-51 0.4162237729275162 1106 52-53 0.24731756453071796 1106 54-55 0.23485252978765914 1106 56-57 0.26611973020721535 1106 58-59 0.4295651284420998 1106 60-61 0.43642920498505333 1106 62-63 0.5068834265757189 1106 64-65 0.31571969830391566 1106 66-67 0.38538117841806496 1106 68-69 0.38846790987239643 1106 70-71 0.3775873377906578 1106 72-73 0.2992958481620178 1106 74-75 0.22713201918976011 1106 76-77 0.2610815815748708 1106 78-79 0.32021050896185344 1106 80-81 0.31227539662311443 1106 82-83 0.4474523885393644 1106 84-85 0.5453522520986489 1106 86-87 0.42750295368922764 1106 88-89 0.6235630484244936 1106 90-91 0.6423725500826265 1106 92-93 0.7334091525053772 1106 94-95 0.6833949840445896 1106 96-97 0.6258239734185054 1106 98-99 0.3571204598292361 1106 100-101 0.3279882023585081 1107 1 0.07784123973619472 1107 2 0.15034673686590594 1107 3 0.15117822186532237 1107 4 0.21084077987045902 1107 5 0.16660841020127037 1107 6 0.16585519718427122 1107 7 0.21773748878469945 1107 8 0.21568746639825065 1107 9 0.23718116137015954 1107 10-11 0.37345648394890674 1107 12-13 0.33687531553805883 1107 14-15 0.30099551544812897 1107 16-17 0.2834930339619106 1107 18-19 0.3319138109771913 1107 20-21 0.2802233587807734 1107 22-23 0.27195483969768475 1107 24-25 0.2624476478967921 1107 26-27 0.28779399096496405 1107 28-29 0.2705506264025388 1107 30-31 0.27865286910729736 1107 32-33 0.33824550351849325 1107 34-35 0.27579180401130543 1107 36-37 0.20912056021219172 1107 38-39 0.3559918058330638 1107 40-41 0.29370727015211173 1107 42-43 0.278199515225829 1107 44-45 0.37181815381865135 1107 46-47 0.4807786916967913 1107 48-49 0.5990719343952478 1107 50-51 0.5054104215557373 1107 52-53 0.5328439335233739 1107 54-55 0.6556173858207188 1107 56-57 0.5442684865890826 1107 58-59 0.5020132249267917 1107 60-61 0.4193714434865612 1107 62-63 0.3538886641316523 1107 64-65 0.36588657375810385 1107 66-67 0.23728065028517165 1107 68-69 0.38709926034869113 1107 70-71 0.5206901343316943 1107 72-73 0.4516227007355269 1107 74-75 0.5592887355882894 1107 76-77 0.5546614486457813 1107 78-79 0.6695618703591499 1107 80-81 0.546845491547554 1107 82-83 0.4917965218201239 1107 84-85 0.48331601914741285 1107 86-87 0.4943694306974358 1107 88-89 0.6067558690518382 1107 90-91 0.4425944200596206 1107 92-93 0.44824409660015263 1107 94-95 0.4369918855066466 1107 96-97 0.47205298136924867 1107 98-99 0.5798195279456557 1107 100-101 0.8176343937513195 1108 1 -0.06762298402812661 1108 2 0.08276851799215024 1108 3 0.03974413220060313 1108 4 0.04846129662592347 1108 5 0.05547374324532939 1108 6 0.1283516180919051 1108 7 0.20497733876189272 1108 8 0.11924433211495256 1108 9 0.22166993248769273 1108 10-11 0.15710765012249794 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1661.0 5 1770.0 6 1972.0 7 2328.0 8 2828.0 9 3394.0 10 3925.0 11 4808.0 12 5906.0 13 8715.0 14 7335.0 15 6919.0 16 7915.0 17 10121.0 18 9607.0 19 7570.0 20 7941.0 21 8508.0 22 9250.0 23 10199.0 24 11692.0 25 13456.0 26 15699.0 27 18513.0 28 22152.0 29 26128.0 30 32039.0 31 39618.0 32 49908.0 33 65659.0 34 92597.0 35 137985.0 36 229212.0 37 385842.0 38 388463.0 39 64350.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.12115285353246 17.708934044594866 12.94859492484211 24.221318177030565 2 15.633020320661856 18.98904184676702 40.93420695069296 24.44373088187817 3 20.206654013037227 23.346701124485353 28.297873946393743 28.148770916083677 4 12.938284766642976 15.097249513404035 35.591010865374336 36.37345485457865 5 14.428607021083462 36.94167565398337 33.486596847266725 15.143120477666445 6 34.16776957369741 33.92553636110337 17.48264372620043 14.424050338998793 7 31.157032133943968 31.012945184842888 19.489066053010294 18.340956628202843 8 27.275682836927857 35.39378908238862 18.765642389503988 18.564885691179537 9 27.211142219097876 16.713502794784617 19.306566016881906 36.7687889692356 10-11 26.12388656212178 25.662199854298905 26.82923077369602 21.384682809883294 12-13 26.698487933509128 22.674873051140906 27.427484228445543 23.199154786904423 14-15 23.29012966325635 24.340741058947554 25.16027948190427 27.208849795891833 16-17 23.489227184695945 27.857414634613537 25.710600652257636 22.942757528432878 18-19 23.299527409851272 26.610880458581672 27.47957435827622 22.61001777329083 20-21 23.95998893387526 25.846194219778695 27.299943943633597 22.89387290271245 22-23 23.604027313266442 25.783253205128204 27.170632432181346 23.442087049424007 24-25 23.59584589256777 25.929315597821496 27.29377037775409 23.181068131856644 26-27 23.30410135250296 26.310641019796698 27.21837808237315 23.166879545327184 28-29 23.06132948688468 26.359003035814244 27.49435575469134 23.08531172260973 30-31 23.161877842898402 26.098402460932462 27.40077943463119 23.338940261537942 32-33 22.992444761060415 26.24486103829524 27.36110651476067 23.40158768588367 34-35 23.639149754260117 26.197998295691427 27.037671387312418 23.12518056273604 36-37 23.631978305726424 25.981521052752743 27.038551570378317 23.34794907114252 38-39 23.3516605969077 26.167172261420017 27.3310452658065 23.150121875865782 40-41 23.372958835205388 26.065949300910614 27.386158598122268 23.174933265761734 42-43 23.35350399290487 25.97003740735205 27.15731803358358 23.519140566159503 44-45 23.292972705220755 26.30115312611543 27.06598969980842 23.339884468855395 46-47 23.302235206972135 26.359362861400616 26.717492521238633 23.620909410388617 48-49 23.52051968035811 26.331287485355574 26.734278736586877 23.41391409769944 50-51 23.428533630247216 26.383704859361718 26.747688567950963 23.440072942440104 52-53 23.627230880952467 26.386144788268112 26.69133734545128 23.295286985328147 54-55 23.155009880311578 26.089675118106292 26.97887402325938 23.776440978322753 56-57 23.347597477314768 26.6675794165198 26.707546207961315 23.277276898204118 58-59 23.44778175484991 26.139295252463178 26.85327516258588 23.55964783010103 60-61 23.513329791337636 26.121733054825725 27.155481480942367 23.209455672894272 62-63 23.337171164756587 26.170096728501203 27.085903136320123 23.40682897042209 64-65 23.22675292028311 26.048724278588146 27.011529592173062 23.71299320895568 66-67 23.138852935887595 26.523959562564098 26.704976164155912 23.632211337392388 68-69 22.99166011803854 26.33181024290941 27.090508647686917 23.58602099136514 70-71 22.99889448850201 26.368416588676567 26.96188943660884 23.670799486212584 72-73 23.1535354359623 26.20154112483461 26.911105541404616 23.733817897798478 74-75 23.081247874173823 26.410288022304744 26.7444161632448 23.764047940276637 76-77 23.158768298674225 26.252037556901524 26.808100457893012 23.781093686531232 78-79 23.1137160030718 26.281975087661646 26.945540821951013 23.658768087315543 80-81 22.92458002742036 26.732665479189137 26.90076521797904 23.441989275411466 82-83 23.22584028869422 26.550660619666928 26.682886534256216 23.540612557382637 84-85 23.14146004891577 26.355891658037155 26.553005779100484 23.94964251394659 86-87 23.144098186924282 26.11210165322272 27.050764546666965 23.693035613186037 88-89 23.17050425878889 26.1252539711589 26.88364128489881 23.8206004851534 90-91 23.070026639619 26.323499408332314 27.039679625063396 23.566794326985292 92-93 23.218091593998807 26.100830280268045 26.99435278853106 23.686725337202088 94-95 23.104046465157545 26.029832319259093 26.925827823834382 23.940293391748984 96-97 23.18462790806799 25.947997450381088 26.9057658168617 23.96160882468922 98-99 23.109371047407215 25.976337775974763 26.71046358048222 24.2038275961358 100-101 23.301501620337252 25.55633618741537 27.21706166606395 23.92510052618343 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1449.0 1 798.0 2 161.5 3 170.0 4 147.5 5 151.0 6 234.5 7 416.0 8 618.0 9 765.0 10 816.0 11 873.0 12 1027.5 13 1156.0 14 1254.0 15 1387.5 16 1522.5 17 1594.5 18 1644.0 19 1681.5 20 1735.5 21 1838.5 22 2000.0 23 2400.5 24 2897.0 25 3586.0 26 4776.5 27 6165.0 28 7673.0 29 9722.0 30 12315.0 31 15415.0 32 18699.5 33 21805.5 34 25624.5 35 29055.0 36 33028.0 37 37606.0 38 40781.0 39 43032.0 40 45108.5 41 49741.5 42 55206.0 43 59373.5 44 63004.5 45 66886.5 46 68845.0 47 69761.5 48 76395.0 49 78052.5 50 73677.0 51 71221.0 52 68463.0 53 67562.0 54 66008.0 55 61134.0 56 54666.0 57 48728.0 58 43598.5 59 38171.5 60 32678.5 61 27222.5 62 22294.0 63 17852.0 64 13992.5 65 11007.0 66 8286.0 67 6978.5 68 5553.0 69 3751.5 70 2789.0 71 2119.5 72 1740.0 73 1388.0 74 1055.0 75 853.0 76 614.0 77 349.5 78 212.0 79 154.0 80 98.5 81 73.0 82 55.5 83 36.5 84 30.0 85 26.0 86 23.0 87 16.5 88 9.0 89 8.5 90 9.5 91 7.5 92 4.0 93 4.5 94 4.5 95 3.0 96 4.0 97 4.5 98 3.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33447085515967834 2 0.11761867422643102 3 0.11118096572587192 4 0.11877862170400924 5 0.13600384174604574 6 0.08676407132285051 7 0.09540568003080821 8 0.10050944893215236 9 0.08948994789515931 10-11 0.08763403193103415 12-13 0.09125886779846608 14-15 0.09279579820625722 16-17 0.08296524333378184 18-19 0.09787056842066191 20-21 0.1068601613718931 22-23 0.11460281078472766 24-25 0.12814519758545334 26-27 0.12681125798623838 28-29 0.12225846413674388 30-31 0.11263090007284471 32-33 0.10642518106780126 34-35 0.32655421362520703 36-37 0.4855250154273015 38-39 0.45936819980791266 40-41 0.43605325550859053 42-43 0.4703297034710268 44-45 0.47723139096261724 46-47 0.4846840535060572 48-49 0.4956455571691714 50-51 0.48384309158481303 52-53 0.4879899038171552 54-55 0.4742445262078533 56-57 0.45185753989059374 58-59 0.4309784852941859 60-61 0.39023533014425105 62-63 0.46218107244103984 64-65 0.49300667665768094 66-67 0.5003723431403027 68-69 0.5129287745850868 70-71 0.5057660989110413 72-73 0.49796545212432775 74-75 0.5049541356767366 76-77 0.4821321690553852 78-79 0.46154310132837184 80-81 0.4624130619365555 82-83 0.47636143035443357 84-85 0.4758684526764628 86-87 0.479319296422258 88-89 0.4912377567543742 90-91 0.5060850844673753 92-93 0.4982264403067829 94-95 0.5042291685032502 96-97 0.5025762433477012 98-99 0.4970374941422652 100-101 0.4854090206795436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1724216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.51563413157962 #Duplication Level Percentage of deduplicated Percentage of total 1 80.10704740987066 46.87514677597095 2 11.66237123300909 13.64862096354838 3 3.5290168598388787 6.195079782435235 4 1.448648575265051 3.3907436006177525 5 0.8228404037294227 2.407451400665608 6 0.4864416610835695 1.7078665359794405 7 0.3508051367414175 1.4369309523127682 8 0.23672680075299274 1.1081775089601174 9 0.1746645192242884 0.9198544592427045 >10 1.0349121656209535 11.203153742443343 >50 0.08977366320312628 3.667816262413248 >100 0.05247823401171891 5.404042444520476 >500 0.003378918140853999 1.2774426718979823 >1k 8.944195078731173E-4 0.7576728989920706 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2340 0.13571385487665116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1970 0.1142548265414542 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 5.799737387891076E-5 9 0.0 0.0 0.0 5.799737387891076E-5 5.799737387891076E-5 10-11 0.0 0.0 0.0 1.1599474775782152E-4 5.799737387891076E-5 12-13 0.0 0.0 0.0 1.1599474775782152E-4 5.799737387891076E-5 14-15 0.0 0.0 0.0 1.1599474775782152E-4 5.799737387891076E-5 16-17 0.0 0.0 0.0 1.1599474775782152E-4 5.799737387891076E-5 18-19 0.0 0.0 0.0 1.739921216367323E-4 1.1599474775782152E-4 20-21 0.0 0.0 0.0 2.0299080857618767E-4 1.1599474775782152E-4 22-23 0.0 0.0 0.0 4.0598161715237535E-4 1.4499343469727693E-4 24-25 0.0 0.0 0.0 6.959684865469292E-4 1.739921216367323E-4 26-27 0.0 0.0 0.0 0.0010439527298203937 2.3198949551564305E-4 28-29 0.0 0.0 0.0 0.0020879054596407874 2.3198949551564305E-4 30-31 0.0 0.0 0.0 0.005596746579314889 2.8998686939455385E-4 32-33 0.0 0.0 0.0 0.01368738023542294 3.479842432734646E-4 34-35 0.0 0.0 0.0 0.025982823497752022 3.479842432734646E-4 36-37 0.0 0.0 0.0 0.049471759918710875 3.479842432734646E-4 38-39 0.0 0.0 0.0 0.08856198991309673 4.349803040918307E-4 40-41 0.0 0.0 0.0 0.12979812274100228 4.639789910312861E-4 42-43 0.0 0.0 0.0 0.1702222923346031 4.639789910312861E-4 44-45 0.0 0.0 0.0 0.22169496165213637 4.639789910312861E-4 46-47 0.0 0.0 0.0 0.2860430479707879 4.639789910312861E-4 48-49 0.0 0.0 0.0 0.35778579945900046 5.219763649101968E-4 50-51 0.0 0.0 0.0 0.4442598839124564 5.219763649101968E-4 52-53 0.0 0.0 0.0 0.5317489224087933 5.509750518496522E-4 54-55 0.0 0.0 0.0 0.6280825604216641 5.799737387891076E-4 56-57 0.0 0.0 0.0 0.7413514316071768 5.799737387891076E-4 58-59 0.0 0.0 0.0 0.8706275779832688 5.799737387891076E-4 60-61 0.0 0.0 0.0 1.0214497487553764 5.799737387891076E-4 62-63 0.0 0.0 0.0 1.1724459116491206 6.379711126680183E-4 64-65 0.0 0.0 0.0 1.3299957777911815 6.379711126680183E-4 66-67 0.0 0.0 0.0 1.5144854240999968 6.379711126680183E-4 68-69 0.0 0.0 0.0 1.7279157599743882 6.379711126680183E-4 70-71 0.0 0.0 0.0 1.9757095398720346 6.379711126680183E-4 72-73 0.0 0.0 0.0 2.225620223916261 7.5396586042584E-4 74-75 0.0 0.0 0.0 2.4866664037452386 7.5396586042584E-4 76-77 0.0 0.0 0.0 2.771404510803751 7.5396586042584E-4 78-79 0.0 0.0 0.0 3.098451702106929 7.5396586042584E-4 80-81 0.0 0.0 0.0 3.4637191627963086 7.829645473652954E-4 82-83 0.0 0.0 0.0 3.8418620404868067 8.119632343047507E-4 84-85 0.0 0.0 0.0 4.221918831515309 8.119632343047507E-4 86-87 0.0 0.0 0.0 4.627175481494198 8.119632343047507E-4 88-89 0.0 0.0 0.0 5.074654219656933 8.119632343047507E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCGC 90 5.823822E-7 36.84335 6 GTACATG 7800 0.0 28.970936 1 TACATGG 7875 0.0 28.096066 2 ACATGGG 7850 0.0 26.978458 3 TACCTGG 1760 0.0 25.842693 2 CATGGGG 3920 0.0 25.502079 4 GTATCAA 6930 0.0 24.524488 1 GTACCTG 2000 0.0 24.211424 1 TATCGCT 160 1.9053241E-6 23.685013 7 GGTATCA 5280 0.0 23.197222 1 TATATCG 110 0.0029211096 21.535578 5 ATGGGGA 2410 0.0 21.231758 5 ATCAACG 7925 0.0 20.804592 3 TCAACGC 7950 0.0 20.798765 4 TATCAAC 8085 0.0 20.627277 2 CAACGCA 8085 0.0 20.627277 5 AACGCAG 8330 0.0 20.187704 6 GCGTTCG 95 8.164554E-6 20.026663 34-35 GAGTACT 3240 0.0 19.881691 12-13 CATGGGA 3720 0.0 19.358864 4 >>END_MODULE