FastQCFastQC Report
Fri 27 May 2016
SRR939103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724216
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.15230110380601966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21640.1255063170739629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20000.031.3165552
GTACCTG22850.028.5382061
GTACATG77350.027.1375471
TACATGG77450.026.650042
ACATGGG79150.024.5189823
CATGGGG38800.023.3577864
GTATCAA70750.022.6723121
ACCTGGG27350.022.0331423
TAGTACG1353.8244724E-421.088594
GGTATCA54850.020.5666731
GAGTACT31850.019.1452712-13
ATCAACG82150.019.1183533
TCAACGC83000.018.922564
CAACGCA84350.018.6759645
TATCAAC84450.018.5976622
AACGCAG86200.018.2751466
ATTCGCG3600.017.81260980-81
TATACAG8300.017.7220365
GTACTTT34300.017.57022914-15
CGCGGAA3700.017.40216682-83
Produced by FastQC (version 0.11.5)