##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939103_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1724216 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5224699225619 34.0 31.0 34.0 31.0 34.0 2 32.7281581889972 34.0 31.0 34.0 31.0 34.0 3 32.82640168053191 34.0 31.0 34.0 31.0 34.0 4 36.27162490082449 37.0 37.0 37.0 35.0 37.0 5 36.233786834132154 37.0 37.0 37.0 35.0 37.0 6 36.27015640731788 37.0 37.0 37.0 35.0 37.0 7 36.22146297215662 37.0 37.0 37.0 35.0 37.0 8 36.206215462563854 37.0 37.0 37.0 35.0 37.0 9 37.96226342871195 39.0 38.0 39.0 35.0 39.0 10-11 37.89479102386244 39.0 38.0 39.0 35.0 39.0 12-13 37.824165591782005 39.0 38.0 39.0 35.0 39.0 14-15 39.21666049961258 41.0 39.0 41.0 36.0 41.0 16-17 39.157994995986584 40.0 39.0 41.0 36.0 41.0 18-19 39.16119210122166 40.5 38.5 41.0 36.0 41.0 20-21 39.227524857674446 41.0 39.0 41.0 36.0 41.0 22-23 39.19159664450394 41.0 39.0 41.0 36.0 41.0 24-25 39.134323077851036 41.0 39.0 41.0 36.0 41.0 26-27 39.01583328306894 40.0 39.0 41.0 35.0 41.0 28-29 38.869206642323235 40.0 38.0 41.0 35.0 41.0 30-31 38.714685689031995 40.0 38.0 41.0 35.0 41.0 32-33 38.622976471625364 40.0 38.0 41.0 34.5 41.0 34-35 38.60776666032562 40.0 38.0 41.0 35.0 41.0 36-37 38.52648334083433 40.0 38.0 41.0 34.0 41.0 38-39 38.4150518264533 40.0 38.0 41.0 34.0 41.0 40-41 38.26669831390035 40.0 38.0 41.0 34.0 41.0 42-43 38.1361937831455 40.0 38.0 41.0 33.5 41.0 44-45 37.957948424095356 40.0 38.0 41.0 33.0 41.0 46-47 37.73006920246651 40.0 37.0 41.0 33.0 41.0 48-49 37.47879761004422 40.0 37.0 41.0 32.5 41.0 50-51 37.256588501672645 40.0 37.0 41.0 32.0 41.0 52-53 37.02452418954469 40.0 36.0 41.0 31.5 41.0 54-55 36.77787788768924 39.0 36.0 41.0 31.0 41.0 56-57 36.50627415590622 39.0 35.0 41.0 31.0 41.0 58-59 36.21127921327722 39.0 35.0 40.0 30.5 41.0 60-61 35.85438802331031 38.0 35.0 40.0 29.5 41.0 62-63 35.45319031954233 38.0 34.5 40.0 29.0 41.0 64-65 35.03932105954243 37.0 34.0 40.0 28.0 41.0 66-67 34.67779587940258 36.5 34.0 39.0 28.0 41.0 68-69 34.212099876117605 36.0 34.0 39.0 27.5 40.0 70-71 33.76901878882924 35.5 33.0 38.5 26.0 40.0 72-73 33.29209130410575 35.0 33.0 37.5 26.0 39.5 74-75 32.79141360479197 35.0 32.5 37.0 25.5 39.0 76-77 30.79562189424063 33.5 29.5 35.0 22.5 37.5 78-79 31.788806912822988 34.5 31.5 36.0 24.0 38.0 80-81 31.81881620400228 35.0 32.0 36.0 25.0 37.0 82-83 31.534915289035713 35.0 32.0 35.0 24.0 37.0 84-85 31.179480413126896 34.0 31.5 35.0 23.0 36.5 86-87 30.631531664246243 34.0 31.0 35.0 19.5 36.0 88-89 30.108495687315276 34.0 30.5 35.0 17.5 36.0 90-91 29.67074542864699 34.0 30.0 35.0 10.5 35.0 92-93 29.3939329527159 34.0 30.0 35.0 7.0 35.0 94-95 29.06948839356554 34.0 29.5 35.0 2.0 35.0 96-97 28.517532606123595 34.0 29.0 35.0 2.0 35.0 98-99 27.746553216070375 34.0 29.0 35.0 2.0 35.0 100-101 24.037769919778032 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21044506037379307 1101 2 0.2369421287271507 1101 3 0.2569791476183738 1101 4 0.1463919818461008 1101 5 0.19652978076738492 1101 6 0.20994387137940862 1101 7 0.20614044716415236 1101 8 0.184960795619034 1101 9 0.271283635017582 1101 10-11 0.2705539598675699 1101 12-13 0.2674259616431769 1101 14-15 0.36958921018613466 1101 16-17 0.42931757504734236 1101 18-19 0.4831884322481059 1101 20-21 0.3915298604888662 1101 22-23 0.31670340234111194 1101 24-25 0.2954134307016645 1101 26-27 0.325198522431144 1101 28-29 0.3630949934963894 1101 30-31 0.3543753753009966 1101 32-33 0.4051675397115133 1101 34-35 0.5579045951444073 1101 36-37 0.606753315704573 1101 38-39 0.5702253654478469 1101 40-41 0.538301025211446 1101 42-43 0.5776241713551471 1101 44-45 0.6751396753512466 1101 46-47 0.6230527323874568 1101 48-49 0.5618818639758985 1101 50-51 0.5995912221776507 1101 52-53 0.4921091142880627 1101 54-55 0.5529842382980945 1101 56-57 0.48315197239885066 1101 58-59 0.5095606047574464 1101 60-61 0.44067287308515546 1101 62-63 0.44289103474219615 1101 64-65 0.4029981209247069 1101 66-67 0.3881628737351761 1101 68-69 0.3938312028008255 1101 70-71 0.3536285472243961 1101 72-73 0.27094430604186215 1101 74-75 0.2675429592885905 1101 76-77 0.4636410581613042 1101 78-79 0.5591035760849863 1101 80-81 0.6967485686949999 1101 82-83 0.6459101530018962 1101 84-85 0.5400299726271562 1101 86-87 0.6348070520903271 1101 88-89 0.7527728326578718 1101 90-91 1.029949856248905 1101 92-93 0.8858154206826221 1101 94-95 0.7726119540160639 1101 96-97 0.7158521076220481 1101 98-99 0.4785013275017853 1101 100-101 0.2430731439087559 1102 1 0.12151345745132858 1102 2 0.1366971274779658 1102 3 0.18870698848218126 1102 4 0.09294347285241855 1102 5 0.0558233067705558 1102 6 0.1678320429949025 1102 7 0.21050766606865068 1102 8 0.08927330115977128 1102 9 0.22250591007267673 1102 10-11 0.17997491159354695 1102 12-13 0.19792364729475054 1102 14-15 0.20832761493786478 1102 16-17 0.3372395059723914 1102 18-19 0.2810080017228884 1102 20-21 0.2691463189134282 1102 22-23 0.26253717221724315 1102 24-25 0.16065692307985557 1102 26-27 0.21898967498471933 1102 28-29 0.21420323014108789 1102 30-31 0.1857402364266605 1102 32-33 0.28205687454565975 1102 34-35 0.35846319082876477 1102 36-37 0.3991078171100142 1102 38-39 0.3639217322476753 1102 40-41 0.40875057026669737 1102 42-43 0.3861889619175898 1102 44-45 0.36556076229356904 1102 46-47 0.36138674180245545 1102 48-49 0.41218596640361227 1102 50-51 0.5072380192071932 1102 52-53 0.5164008180048896 1102 54-55 0.3434998871260575 1102 56-57 0.3756484957272548 1102 58-59 0.2672668163808538 1102 60-61 0.3094172148189145 1102 62-63 0.4308699893497945 1102 64-65 0.4709232845051261 1102 66-67 0.3885018100393225 1102 68-69 0.3622051323755002 1102 70-71 0.31539254726379795 1102 72-73 0.1855690717638936 1102 74-75 0.26893698595038273 1102 76-77 0.33410425917067954 1102 78-79 0.22673153237518306 1102 80-81 0.1421751224963259 1102 82-83 0.2996457504476524 1102 84-85 0.34331550564366964 1102 86-87 0.4201468704347988 1102 88-89 0.42745308184992936 1102 90-91 0.6409403024538491 1102 92-93 0.6064295983389769 1102 94-95 0.6304384723566798 1102 96-97 0.5604280969623439 1102 98-99 0.17489163347574888 1102 100-101 0.08791262600333738 1103 1 0.2289883106583659 1103 2 0.150416475414481 1103 3 0.15952285460768678 1103 4 0.10770614972661008 1103 5 0.170303857237748 1103 6 0.16742967720377777 1103 7 0.17039551675972575 1103 8 0.1540640281890049 1103 9 0.09663126689972756 1103 10-11 0.24264220767832967 1103 12-13 0.1741877001658736 1103 14-15 0.2813980550345363 1103 16-17 0.22861314258947374 1103 18-19 0.2275025447704664 1103 20-21 0.24239284214802126 1103 22-23 0.27673179069238074 1103 24-25 0.16942174134392474 1103 26-27 0.2292801480232214 1103 28-29 0.3006648399121232 1103 30-31 0.2795000176354492 1103 32-33 0.3185588491739466 1103 34-35 0.3410502874153707 1103 36-37 0.43505049901270354 1103 38-39 0.4022907953211856 1103 40-41 0.5116110446906035 1103 42-43 0.43501119748074046 1103 44-45 0.5269921839404148 1103 46-47 0.43511311082961157 1103 48-49 0.39469203396001973 1103 50-51 0.35503578272044223 1103 52-53 0.5479737047934421 1103 54-55 0.3967068835872851 1103 56-57 0.4928699226249549 1103 58-59 0.5473373522585447 1103 60-61 0.5870168997677823 1103 62-63 0.5880318866349157 1103 64-65 0.5510552228563554 1103 66-67 0.4343090815289088 1103 68-69 0.14997898484195105 1103 70-71 0.13529686886909076 1103 72-73 0.1350253434535844 1103 74-75 0.19470028369080694 1103 76-77 0.23781440528804154 1103 78-79 0.3457411566570414 1103 80-81 0.4694246508603328 1103 82-83 0.37810515554491175 1103 84-85 0.41683213989872314 1103 86-87 0.5806940280475921 1103 88-89 0.5810886128444963 1103 90-91 0.677946410204072 1103 92-93 0.5697454687404182 1103 94-95 0.5357651369366927 1103 96-97 0.6939951530814703 1103 98-99 0.6424293515272197 1103 100-101 0.3654669860065525 1104 1 0.08523738962943384 1104 2 0.14754040906765198 1104 3 0.15699800729381508 1104 4 0.09783517564640931 1104 5 0.1532373957899722 1104 6 0.12078304121078531 1104 7 0.08401118980673772 1104 8 0.015094270685686695 1104 9 0.14751872944454902 1104 10-11 0.05684897662696642 1104 12-13 0.06570973117793955 1104 14-15 0.08438505419947262 1104 16-17 0.15870909333693817 1104 18-19 0.07922875766323756 1104 20-21 0.018556204026431544 1104 22-23 0.09789130471657614 1104 24-25 0.15162255021199655 1104 26-27 0.040931778039755784 1104 28-29 0.039420100010694625 1104 30-31 0.11507138305061204 1104 32-33 0.08216387921996215 1104 34-35 0.08394894967201338 1104 36-37 0.07534981294848109 1104 38-39 0.21298496459402116 1104 40-41 0.29229113245442306 1104 42-43 0.14139044854094607 1104 44-45 0.13576324480200697 1104 46-47 0.1032942219589188 1104 48-49 -0.02402695806591737 1104 50-51 0.024346689340390526 1104 52-53 -0.08170594087649619 1104 54-55 -0.010010078081535312 1104 56-57 0.023371944795144373 1104 58-59 -0.005129367896024917 1104 60-61 0.1237643167933058 1104 62-63 0.029583390572831547 1104 64-65 0.040228834763425425 1104 66-67 0.022662363666675844 1104 68-69 -0.19089546049396233 1104 70-71 -0.21813508960683237 1104 72-73 -0.22586326526057832 1104 74-75 -0.21520343808477094 1104 76-77 -0.17085800314121258 1104 78-79 -0.03955267203976476 1104 80-81 -0.19914902326734918 1104 82-83 -0.2374946184959903 1104 84-85 -0.2966165199018427 1104 86-87 -0.3106323354845735 1104 88-89 -0.2270798904220861 1104 90-91 0.026344229207559522 1104 92-93 0.10730216318804864 1104 94-95 -0.08728637518814608 1104 96-97 0.04406146716461379 1104 98-99 0.08180477110706619 1104 100-101 -0.16754228159124196 1105 1 0.04064744620260541 1105 2 0.034718476085096484 1105 3 0.1167519337882581 1105 4 0.05292082663347486 1105 5 0.011080494258933982 1105 6 0.06677742345551252 1105 7 0.013445062497325466 1105 8 0.11680333426127021 1105 9 0.06106068458162639 1105 10-11 0.07030191803615082 1105 12-13 0.08772974937630096 1105 14-15 0.14804569430707915 1105 16-17 0.1353360664745793 1105 18-19 0.1158054874966794 1105 20-21 0.1217686105620146 1105 22-23 0.1403033789342416 1105 24-25 0.0938664873880839 1105 26-27 0.14431232161692265 1105 28-29 0.11573453605927142 1105 30-31 0.044040406273303745 1105 32-33 0.1573092683088504 1105 34-35 0.27543132495389244 1105 36-37 0.1639580338607729 1105 38-39 0.25901890849559095 1105 40-41 0.19408920975789812 1105 42-43 0.27555391730701473 1105 44-45 0.318678543294709 1105 46-47 0.3258582129388543 1105 48-49 0.32965227602040414 1105 50-51 0.39018124249147945 1105 52-53 0.3220979124288803 1105 54-55 0.4164289771861007 1105 56-57 0.3476153281080414 1105 58-59 0.36512224424149053 1105 60-61 0.2918281693074576 1105 62-63 0.2222704183055484 1105 64-65 0.20372331591229198 1105 66-67 0.27044888896636365 1105 68-69 0.1894938262093646 1105 70-71 0.32558251928662685 1105 72-73 0.27605745317911357 1105 74-75 0.17071459016224821 1105 76-77 0.2022222674605203 1105 78-79 0.4340636590121818 1105 80-81 0.2855880895229319 1105 82-83 0.25168329027482983 1105 84-85 0.32108336536935056 1105 86-87 0.3083493474683081 1105 88-89 0.379956150247299 1105 90-91 0.36193785186847904 1105 92-93 0.5168642156468017 1105 94-95 0.18703810952127853 1105 96-97 0.16136969203307316 1105 98-99 0.19257345409950943 1105 100-101 -0.13318100454095827 1106 1 0.03551067379388684 1106 2 0.04917814939464904 1106 3 0.03439459743044182 1106 4 -0.04560895139634624 1106 5 0.02045665574195965 1106 6 0.11317352125573166 1106 7 0.11727484320933002 1106 8 0.07197784888111158 1106 9 0.14096305295327483 1106 10-11 0.1610920521838466 1106 12-13 0.15448307624684077 1106 14-15 0.13399447985085544 1106 16-17 0.1421273654580304 1106 18-19 -0.021653178076675772 1106 20-21 0.2105329267382885 1106 22-23 0.18636995083029007 1106 24-25 0.21529697466493758 1106 26-27 0.18380772549689794 1106 28-29 0.1743087234522207 1106 30-31 0.2997281766473918 1106 32-33 0.2859684427315159 1106 34-35 0.1239800698816822 1106 36-37 0.21651454180580032 1106 38-39 0.3308111079277367 1106 40-41 0.1651495551470319 1106 42-43 0.16549073263073666 1106 44-45 0.2804991355983617 1106 46-47 0.2468202753112152 1106 48-49 0.31582655690674954 1106 50-51 0.4046162013582091 1106 52-53 0.4524062490510872 1106 54-55 0.41327664042594847 1106 56-57 0.3075888510848088 1106 58-59 0.37225897066984714 1106 60-61 0.40959440516370194 1106 62-63 0.474602388832146 1106 64-65 0.5157228484453142 1106 66-67 0.44781275836681544 1106 68-69 0.3360749949534494 1106 70-71 0.4211331532408167 1106 72-73 0.30984324601460145 1106 74-75 0.4476008385864674 1106 76-77 0.5378267750683534 1106 78-79 0.5667804085073556 1106 80-81 0.5097461699962267 1106 82-83 0.2515537789310933 1106 84-85 0.39843803869806393 1106 86-87 0.5871558506237129 1106 88-89 0.7862487539234273 1106 90-91 0.8142157954735687 1106 92-93 0.5878410300509174 1106 94-95 0.5535492731692031 1106 96-97 0.4163344212662352 1106 98-99 0.42835345340980524 1106 100-101 -0.04427108389539214 1107 1 0.06741640001278881 1107 2 0.06463559810136132 1107 3 0.0057495919903374215 1107 4 0.04275361404528155 1107 5 0.059488856008826474 1107 6 0.07322950623638746 1107 7 0.03992119819461948 1107 8 0.05508071219458799 1107 9 0.15348140146311806 1107 10-11 0.06027364465458618 1107 12-13 0.055202475087149594 1107 14-15 0.117914934586004 1107 16-17 0.1324366442674645 1107 18-19 0.05477195907577226 1107 20-21 0.0683606783284958 1107 22-23 0.06006598710700928 1107 24-25 0.025723342656014836 1107 26-27 0.1058095409653319 1107 28-29 0.13603105174782826 1107 30-31 0.21716145892453653 1107 32-33 0.16687553147913547 1107 34-35 0.2127772126148173 1107 36-37 0.2035790510302249 1107 38-39 0.24504543393229739 1107 40-41 0.2644006651460913 1107 42-43 0.19758101045231058 1107 44-45 0.1459016639738664 1107 46-47 0.16957513551012937 1107 48-49 0.07748975449789697 1107 50-51 0.09545925162710489 1107 52-53 0.07941929898240829 1107 54-55 -0.005744898508126539 1107 56-57 -0.05023055395050591 1107 58-59 -0.09963817900020189 1107 60-61 0.13931904675336426 1107 62-63 -0.14116933548819333 1107 64-65 -0.11687386123146126 1107 66-67 0.058502358151365286 1107 68-69 -8.67079584622843E-4 1107 70-71 0.22920913137359378 1107 72-73 0.18499195380098143 1107 74-75 0.19939377131731106 1107 76-77 0.048982780340409704 1107 78-79 0.2700169799206691 1107 80-81 0.09833576173098635 1107 82-83 0.16727249335390226 1107 84-85 0.22822118036357963 1107 86-87 0.25437258617109393 1107 88-89 0.28619440857711353 1107 90-91 0.39673558701001355 1107 92-93 0.27162456418999525 1107 94-95 0.2118057038187935 1107 96-97 0.30988468588912 1107 98-99 0.4734222531344088 1107 100-101 -0.04262625614417104 1108 1 -6.643959844367942E-4 1108 2 -0.08256534815934202 1108 3 -0.026252537237809292 1108 4 -0.005979732336179211 1108 5 0.02246095554599492 1108 6 0.008696126090235623 1108 7 -0.021740413796727864 1108 8 -0.015998204093406798 1108 9 0.0081651194154162 1108 10-11 0.006959944862302336 1108 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0.10542223641868631 2316 84-85 -0.006796972228588771 2316 86-87 -0.23509896612573655 2316 88-89 -0.3156282103639718 2316 90-91 -0.34582237987736164 2316 92-93 -0.3051015350363677 2316 94-95 0.0739890161462462 2316 96-97 -0.12137103079049538 2316 98-99 0.06927764124694846 2316 100-101 0.28805938857462365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 11.0 5 31.0 6 41.0 7 131.0 8 310.0 9 596.0 10 985.0 11 1545.0 12 2262.0 13 3230.0 14 3971.0 15 5085.0 16 6742.0 17 9427.0 18 9196.0 19 6882.0 20 6954.0 21 7638.0 22 8054.0 23 9026.0 24 10487.0 25 12503.0 26 15170.0 27 18510.0 28 22851.0 29 27951.0 30 34208.0 31 42901.0 32 53328.0 33 71108.0 34 99107.0 35 146979.0 36 243128.0 37 423531.0 38 386540.0 39 33782.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56015810773534 18.245241578702945 13.223423782038523 23.971176531523195 2 16.073218204679694 19.452551188482186 40.15059598101398 24.32363462582414 3 20.30053079196574 23.44741030126156 28.595083214632037 27.656975692140662 4 13.261389524282341 15.621766646406252 35.29627378472303 35.82057004458838 5 14.37261920780227 37.10915569743002 33.4766061792722 15.041618915495505 6 34.18458012221207 34.31571218455228 17.097625819502895 14.402081873732758 7 31.063103462675212 31.231063857428538 19.343458128215953 18.3623745516803 8 27.28527052295072 35.75833886241631 18.595929976290673 18.360460638342296 9 27.142712977956357 16.77933623165543 19.157112565943013 36.9208382244452 10-11 26.13344267771555 25.901888162504 26.593912827627165 21.370756332153277 12-13 26.601858467848576 22.856243069313823 27.405963057992732 23.13593540484487 14-15 23.136344220227542 24.52145156140665 25.121353486852005 27.220850731513803 16-17 23.394539975808883 28.076980071471386 25.634987862391917 22.893492090327815 18-19 23.16364258517644 26.74008171834719 27.522640737959016 22.573634958517356 20-21 23.803630171625827 26.035282122425496 27.351735513415953 22.80935219253272 22-23 23.509525488685874 25.899742259670482 27.261636593095062 23.32909565854858 24-25 23.504099263895622 26.097399855702196 27.37720187540743 23.021299004994745 26-27 23.315209936674925 26.446490934773458 27.253735576269207 22.98456355228241 28-29 22.962958237251016 26.495462285467713 27.57656233325755 22.96501714402372 30-31 23.181984270601337 26.185069831106162 27.455659567557532 23.177286330734965 32-33 22.962610253007743 26.32521099444617 27.45212896760035 23.26004978494574 34-35 23.579775406680607 26.28016190950434 27.121495061481703 23.01856762233335 36-37 23.53691770619861 26.035362215424957 27.205149290855747 23.222570787520684 38-39 23.235634752660676 26.26860182387571 27.45173394653209 23.04402947693152 40-41 23.365870239461806 26.14001465204865 27.4957351865526 22.998379921936948 42-43 23.322530266712487 26.10595125714344 27.162969697760648 23.408548778383427 44-45 23.210160021969998 26.401029597148074 27.106847673619093 23.281962707262828 46-47 23.2941364110892 26.480918686927268 26.728221784015776 23.49672311796775 48-49 23.53642095653856 26.391038428401703 26.767501456467073 23.305039158592663 50-51 23.395415655579114 26.499203116082903 26.76381613440543 23.341565093932548 52-53 23.659788268802984 26.479101063121785 26.670260573727923 23.190850094347308 54-55 23.154378569738363 26.234213114830162 26.897848065439593 23.71356024999188 56-57 23.290904387849316 26.73101861947691 26.701903937789695 23.276173054884076 58-59 23.44450527971529 26.246182167867456 26.84105638385612 23.46825616856113 60-61 23.496360314376034 26.21843914039616 27.139700124706362 23.145500420521447 62-63 23.323932552174337 26.331128862234817 26.980551257940515 23.36438732765033 64-65 23.209830717366874 26.187987095371007 26.922361982093012 23.679820205169104 66-67 23.092214870784343 26.626067009733767 26.711181290518095 23.570536828963796 68-69 22.943748051590266 26.459947945741774 26.98704443461681 23.609259568051154 70-71 22.98688215397607 26.435956979867953 26.89816705099593 23.678993815160048 72-73 23.062655722948865 26.316917369981486 26.7900309473987 23.83039595967095 74-75 23.002976425227466 26.466115614284984 26.653476130600808 23.877431829886742 76-77 23.034102315665397 26.36017225235237 26.78188130304057 23.823844128941662 78-79 23.01085714617129 26.297347770863176 26.944484720538682 23.747310362426852 80-81 22.80207033189341 26.768476157775805 26.83459500466312 23.594858505667663 82-83 23.029895993007816 26.575894713582038 26.636850130291478 23.757359163118664 84-85 22.913167491775972 26.36783906424717 26.549660831357556 24.1693326126193 86-87 22.959105026749395 26.11210505490818 27.038296817389707 23.890493100952725 88-89 22.9100838447213 26.116221432057124 26.805066555168754 24.16862816805282 90-91 22.790430377963215 26.272005300865146 27.053510265622606 23.884054055549036 92-93 22.795171834619328 26.09847026126657 26.969184835310656 24.137173068803445 94-95 22.664735740765657 25.99900476506424 26.93464739916577 24.40161209500434 96-97 22.65116435527799 25.905252010188978 26.963037113679494 24.480546520853537 98-99 22.552256980139433 25.949928531886318 26.742327073699006 24.755487414275237 100-101 22.64649778129888 25.34802955793591 27.384066994276207 24.621405666489 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 105.0 1 82.0 2 76.5 3 104.5 4 92.5 5 83.0 6 155.0 7 313.0 8 525.5 9 627.5 10 622.5 11 647.0 12 690.0 13 771.5 14 842.5 15 935.0 16 1035.5 17 1069.5 18 1148.0 19 1228.0 20 1347.5 21 1604.0 22 1892.0 23 2262.5 24 2846.5 25 3655.0 26 4852.5 27 6383.0 28 7875.5 29 9849.5 30 12526.0 31 15601.0 32 18980.0 33 22189.5 34 25835.0 35 29327.5 36 33371.0 37 37901.0 38 40985.0 39 43265.5 40 45495.5 41 50161.0 42 55604.0 43 59592.0 44 63459.5 45 67619.5 46 68927.0 47 70120.0 48 77775.5 49 79073.5 50 74344.0 51 72051.0 52 68898.0 53 68072.5 54 66603.5 55 61170.0 56 54562.0 57 48617.0 58 43603.5 59 38165.0 60 32305.5 61 26645.0 62 21882.5 63 17579.0 64 13794.0 65 10910.0 66 8092.0 67 6693.5 68 5197.5 69 3285.0 70 2443.0 71 1932.5 72 1564.5 73 1259.5 74 994.5 75 829.0 76 559.5 77 277.0 78 156.0 79 101.0 80 60.5 81 36.5 82 21.0 83 9.5 84 5.5 85 3.5 86 4.0 87 4.0 88 4.0 89 2.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3418365216423 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010149540428809384 16-17 0.0017109225294278674 18-19 5.799737387891076E-5 20-21 0.0 22-23 0.0 24-25 2.3198949551564305E-4 26-27 0.0010149540428809384 28-29 0.0 30-31 0.003247852937219003 32-33 0.0 34-35 0.009656562750838642 36-37 0.02804173027045335 38-39 0.02085005590946842 40-41 0.01304940912275492 42-43 0.009772557498596464 44-45 0.0026968778853693506 46-47 9.279579820625722E-4 48-49 5.509750518496523E-4 50-51 0.0 52-53 8.699606081836615E-5 54-55 0.0 56-57 0.0 58-59 0.0040598161715237535 60-61 0.009627564063899186 62-63 0.003972820110705387 64-65 0.0016529251555489567 66-67 0.0036828332413108332 68-69 0.003102859502521726 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2155762387079113 78-79 3.479842432734646E-4 80-81 0.002783873946187717 82-83 2.899868693945538E-4 84-85 0.0 86-87 0.10674416662413526 88-89 0.4056336329091019 90-91 0.3920912461083762 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0011889461645176707 100-101 5.219763649101968E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1724216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.08267450626307 #Duplication Level Percentage of deduplicated Percentage of total 1 79.05589887407531 45.12722143228889 2 12.221865936090165 13.953135901780382 3 3.6554253825450322 6.259843718812505 4 1.6032564068005821 3.66072654477914 5 0.8881104683306202 2.534786039466082 6 0.5487037908733825 1.8792887936866758 7 0.35332395997238936 1.4118073621657474 8 0.2510975317021128 1.1466654937182221 9 0.18526601706971382 0.9517931774516024 >10 1.082637420649299 11.528288501628827 >50 0.09147654929813391 3.603261388846425 >100 0.058573072618184036 5.844532955619208 >500 0.003248066958161851 1.1814567635576292 >1k 0.0011165230168681366 0.9171919261986792 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2626 0.15230110380601966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2164 0.1255063170739629 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.799737387891076E-5 7 0.0 0.0 0.0 0.0 5.799737387891076E-5 8 0.0 0.0 0.0 0.0 5.799737387891076E-5 9 0.0 0.0 0.0 5.799737387891076E-5 1.739921216367323E-4 10-11 0.0 0.0 0.0 8.699606081836615E-5 1.739921216367323E-4 12-13 0.0 0.0 0.0 1.1599474775782152E-4 1.739921216367323E-4 14-15 0.0 0.0 0.0 1.1599474775782152E-4 1.739921216367323E-4 16-17 0.0 0.0 0.0 1.4499343469727693E-4 1.739921216367323E-4 18-19 0.0 0.0 0.0 2.0299080857618767E-4 1.739921216367323E-4 20-21 0.0 0.0 0.0 2.609881824550984E-4 2.3198949551564305E-4 22-23 0.0 0.0 0.0 4.0598161715237535E-4 3.479842432734646E-4 24-25 0.0 0.0 0.0 6.959684865469292E-4 4.639789910312861E-4 26-27 0.0 0.0 0.0 9.85955355941483E-4 4.639789910312861E-4 28-29 0.0 0.0 0.0 0.002058906772701332 4.639789910312861E-4 30-31 0.0 0.0 0.0 0.005596746579314889 4.929776779707415E-4 32-33 0.0 0.0 0.0 0.01368738023542294 5.219763649101968E-4 34-35 0.0 0.0 0.0 0.026040820871630933 5.799737387891076E-4 36-37 0.0 0.0 0.0 0.04967475072728707 5.799737387891076E-4 38-39 0.0 0.0 0.0 0.08914196365188584 5.799737387891076E-4 40-41 0.0 0.0 0.0 0.13107406496633833 6.379711126680183E-4 42-43 0.0 0.0 0.0 0.1719622135509704 6.379711126680183E-4 44-45 0.0 0.0 0.0 0.22372486973789826 6.379711126680183E-4 46-47 0.0 0.0 0.0 0.2882759468651259 6.379711126680183E-4 48-49 0.0 0.0 0.0 0.3605116760313093 6.959684865469292E-4 50-51 0.0 0.0 0.0 0.4475657342235544 6.959684865469292E-4 52-53 0.0 0.0 0.0 0.5349677766590729 6.959684865469292E-4 54-55 0.0 0.0 0.0 0.6319683844715511 6.959684865469292E-4 56-57 0.0 0.0 0.0 0.745759232021974 7.249671734863845E-4 58-59 0.0 0.0 0.0 0.875412361328279 7.5396586042584E-4 60-61 0.0 0.0 0.0 1.0261475360395682 7.5396586042584E-4 62-63 0.0 0.0 0.0 1.1775786792374041 8.119632343047507E-4 64-65 0.0 0.0 0.0 1.3360275046745884 8.40961921244206E-4 66-67 0.0 0.0 0.0 1.5215901024001632 8.699606081836614E-4 68-69 0.0 0.0 0.0 1.7353684225178285 8.699606081836614E-4 70-71 0.0 0.0 0.0 1.9839451669628398 8.699606081836614E-4 72-73 0.0 0.0 0.0 2.2340588418156426 9.279579820625722E-4 74-75 0.0 0.0 0.0 2.4953080124531963 9.85955355941483E-4 76-77 0.0 0.0 0.0 2.781989031536652 9.85955355941483E-4 78-79 0.0 0.0 0.0 3.1094132057700428 9.85955355941483E-4 80-81 0.0 0.0 0.0 3.4746516677724832 0.0010149540428809384 82-83 0.0 0.0 0.0 3.8546794601140464 0.0010439527298203937 84-85 0.0 0.0 0.0 4.235258227507458 0.0010439527298203937 86-87 0.0 0.0 0.0 4.6421968013288355 0.0010439527298203937 88-89 0.0 0.0 0.0 5.0915894528295755 0.0010439527298203937 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2000 0.0 31.316555 2 GTACCTG 2285 0.0 28.538206 1 GTACATG 7735 0.0 27.137547 1 TACATGG 7745 0.0 26.65004 2 ACATGGG 7915 0.0 24.518982 3 CATGGGG 3880 0.0 23.357786 4 GTATCAA 7075 0.0 22.672312 1 ACCTGGG 2735 0.0 22.033142 3 TAGTACG 135 3.8244724E-4 21.08859 4 GGTATCA 5485 0.0 20.566673 1 GAGTACT 3185 0.0 19.14527 12-13 ATCAACG 8215 0.0 19.118353 3 TCAACGC 8300 0.0 18.92256 4 CAACGCA 8435 0.0 18.675964 5 TATCAAC 8445 0.0 18.597662 2 AACGCAG 8620 0.0 18.275146 6 ATTCGCG 360 0.0 17.812609 80-81 TATACAG 830 0.0 17.722036 5 GTACTTT 3430 0.0 17.570229 14-15 CGCGGAA 370 0.0 17.402166 82-83 >>END_MODULE