FastQCFastQC Report
Fri 27 May 2016
SRR939102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692181
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.137455744982363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.1138176117093857No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18400.032.6944732
CACGTTA600.00401829331.5583727
GTACCTG22200.029.0916331
GTACATG76850.028.9808481
TACATGG76800.028.1866472
ACATGGG78000.026.174063
CATGGGG39650.024.1321874
ACCTGGG27550.021.4920023
GGTATCA54000.021.3695141
GTATCAA67550.020.879171
CCCTATA2108.845109E-720.300732
ATGGGGA21700.020.300135
GTACCGT1656.493855E-520.08266
ATTCGCG2700.019.3733280-81
ACCGTAT1507.9030887E-418.9350228
CATGGGA38400.018.749984
GCACCGT5150.018.3835186
AGTACAT54400.018.372662
CGCGGAA2900.018.03830182-83
TCTACGG1600.001213707417.763142
Produced by FastQC (version 0.11.5)