##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939102_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1692181 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13579457516661 33.0 31.0 34.0 30.0 34.0 2 32.23019759706556 34.0 31.0 34.0 30.0 34.0 3 32.304402425036095 34.0 31.0 34.0 30.0 34.0 4 35.72828083993379 37.0 35.0 37.0 35.0 37.0 5 35.76390527963616 37.0 35.0 37.0 35.0 37.0 6 35.84291869486775 37.0 37.0 37.0 35.0 37.0 7 35.767712200999775 37.0 36.0 37.0 35.0 37.0 8 35.76651847526949 37.0 36.0 37.0 35.0 37.0 9 37.449862041944684 39.0 38.0 39.0 35.0 39.0 10-11 37.348592437806595 39.0 38.0 39.0 34.5 39.0 12-13 37.2531729761769 39.0 37.0 39.0 34.0 39.0 14-15 38.578961115861716 40.0 38.0 41.0 34.0 41.0 16-17 38.48667252498403 40.0 38.0 41.0 34.0 41.0 18-19 38.348673398413055 40.0 38.0 41.0 34.0 41.0 20-21 38.32599083667764 40.0 38.0 41.0 34.0 41.0 22-23 38.37541462763144 40.0 38.0 41.0 34.0 41.0 24-25 38.32589273842456 40.0 38.0 41.0 34.0 41.0 26-27 38.22118999090523 40.0 38.0 41.0 34.0 41.0 28-29 38.08999628290354 40.0 38.0 41.0 33.0 41.0 30-31 37.91759273978374 40.0 38.0 41.0 33.0 41.0 32-33 37.76286638367881 40.0 38.0 41.0 33.0 41.0 34-35 37.47823341592891 40.0 37.5 41.0 31.5 41.0 36-37 37.2363845829731 40.0 37.0 41.0 31.0 41.0 38-39 37.07711704598976 40.0 37.0 41.0 31.0 41.0 40-41 36.909842091360204 40.0 37.0 41.0 30.5 41.0 42-43 36.73076373035745 40.0 36.5 41.0 30.0 41.0 44-45 36.5058111395885 40.0 36.0 41.0 30.0 41.0 46-47 36.45391125417434 40.0 36.0 41.0 30.0 41.0 48-49 36.39154056215027 40.0 36.0 41.0 30.0 41.0 50-51 35.28644364875862 38.5 34.5 40.0 27.5 40.5 52-53 35.43376565509245 38.5 35.0 39.5 28.0 40.5 54-55 35.960065737648634 39.0 35.0 41.0 29.0 41.0 56-57 35.87551508969785 39.0 35.0 41.0 28.0 41.0 58-59 35.674140945915354 39.0 35.0 41.0 28.0 41.0 60-61 35.37862232231659 38.5 35.0 41.0 28.0 41.0 62-63 34.99518638963562 38.0 35.0 40.0 26.5 41.0 64-65 34.62066173772191 37.5 34.0 40.0 26.0 41.0 66-67 34.235801902987916 37.0 34.0 40.0 26.0 41.0 68-69 33.8300391624773 36.0 34.0 39.0 26.0 41.0 70-71 33.400356699431086 36.0 33.5 39.0 25.0 40.5 72-73 32.94987504291799 35.0 33.0 38.5 24.0 40.0 74-75 32.49373057610268 35.0 33.0 37.0 22.5 39.5 76-77 32.05004311004556 35.0 32.0 37.0 21.5 39.0 78-79 31.62314167337891 35.0 32.0 36.0 20.0 39.0 80-81 31.18142208191677 35.0 31.5 36.0 19.0 37.5 82-83 30.795919880911086 35.0 31.0 35.5 17.5 37.0 84-85 30.42381754670452 34.5 31.0 35.0 12.0 36.5 86-87 30.016244716138523 34.0 31.0 35.0 7.0 36.0 88-89 29.616413374219423 34.0 30.0 35.0 2.0 36.0 90-91 29.22494136265565 34.0 30.0 35.0 2.0 35.5 92-93 28.830711076415582 34.0 29.0 35.0 2.0 35.0 94-95 28.359846553057857 34.0 29.0 35.0 2.0 35.0 96-97 27.76511407467641 34.0 28.5 35.0 2.0 35.0 98-99 27.048242179766824 34.0 27.0 35.0 2.0 35.0 100-101 24.08387902948916 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1889535181744293 1101 2 0.3241360832647473 1101 3 0.2131319791698374 1101 4 0.3608672664360526 1101 5 0.2603091624861449 1101 6 0.1709177390302017 1101 7 0.15273691708507897 1101 8 0.32426764851621215 1101 9 0.27432583698637814 1101 10-11 0.2429793665583233 1101 12-13 0.40134520023715936 1101 14-15 0.44107265648096217 1101 16-17 0.5275636000868857 1101 18-19 0.4792490058008667 1101 20-21 0.4331112583169485 1101 22-23 0.4938390358314919 1101 24-25 0.4657208594165283 1101 26-27 0.48241621459153095 1101 28-29 0.4219417112016757 1101 30-31 0.5445128663121679 1101 32-33 0.47573829412053925 1101 34-35 0.5510716612257198 1101 36-37 0.2800113752134834 1101 38-39 0.33422069348758754 1101 40-41 0.380964261632748 1101 42-43 0.3642540517806765 1101 44-45 0.2386048200521813 1101 46-47 0.5658119036124774 1101 48-49 0.7218858371114862 1101 50-51 0.4493307206639514 1101 52-53 0.5165549089512282 1101 54-55 0.5888277276709388 1101 56-57 0.616621956237708 1101 58-59 0.5976578555292278 1101 60-61 0.5244365186495159 1101 62-63 0.31124871513053876 1101 64-65 0.2608249511305729 1101 66-67 0.43845222655471616 1101 68-69 0.23014063530387574 1101 70-71 0.32469050415429734 1101 72-73 0.2539709955829821 1101 74-75 0.175215831227284 1101 76-77 0.1854660582352352 1101 78-79 0.028293983974698733 1101 80-81 -0.23208577846534695 1101 82-83 -0.21405186786910946 1101 84-85 -0.18728520091535472 1101 86-87 -0.31568098920933707 1101 88-89 -0.5389245280178976 1101 90-91 -0.6282735245953361 1101 92-93 -0.8205533811147987 1101 94-95 -0.9746796311770964 1101 96-97 -0.9630639815116275 1101 98-99 -1.0949988231695862 1101 100-101 -1.1156589890743938 1102 1 0.12901743718571623 1102 2 0.13780339035310618 1102 3 0.19408374433212572 1102 4 0.02261377028781908 1102 5 0.19834374525756715 1102 6 0.11992801040889844 1102 7 0.16828484710669045 1102 8 0.27712045005322494 1102 9 0.3003215695610564 1102 10-11 0.29409587096429846 1102 12-13 0.30944291688751946 1102 14-15 0.2567628340132373 1102 16-17 0.18214438558872104 1102 18-19 0.2549667091501533 1102 20-21 0.16192007396787034 1102 22-23 0.28966236257586075 1102 24-25 0.3459362166055655 1102 26-27 0.26038378400647133 1102 28-29 0.18205352328716629 1102 30-31 0.24127581175932988 1102 32-33 0.23757647372188018 1102 34-35 0.2426222514605243 1102 36-37 0.2606645591561332 1102 38-39 -0.0055268633652332255 1102 40-41 0.12430818655562348 1102 42-43 0.20373073796788788 1102 44-45 0.1658145058144953 1102 46-47 0.24262717582248428 1102 48-49 0.2911947820783283 1102 50-51 0.14268203506789945 1102 52-53 0.33331679216048116 1102 54-55 0.3823974936091261 1102 56-57 0.32371655083230166 1102 58-59 0.28332942080605505 1102 60-61 0.2808900757609649 1102 62-63 0.12718066724669796 1102 64-65 0.16716050746612865 1102 66-67 0.15827220032205958 1102 68-69 0.20451645507443317 1102 70-71 0.027638445260130595 1102 72-73 0.04252145650186634 1102 74-75 -0.03888168221234167 1102 76-77 -0.09041213540611892 1102 78-79 -0.24348766609642425 1102 80-81 -0.17134632233115354 1102 82-83 -0.4126922033515541 1102 84-85 -0.29832208366276447 1102 86-87 -0.23596557659918815 1102 88-89 -0.2324344853436493 1102 90-91 -0.30641567050063756 1102 92-93 -0.4194103207612123 1102 94-95 -0.4770456506483782 1102 96-97 -0.5968770922984241 1102 98-99 -0.633815305407122 1102 100-101 -1.2076259303834398 1103 1 0.1851329049337025 1103 2 0.16369833685281066 1103 3 0.24747864617382476 1103 4 0.2625523208674778 1103 5 0.3468360908748309 1103 6 0.23809086923599665 1103 7 0.1738693311280528 1103 8 0.221670833218937 1103 9 0.2612114250477404 1103 10-11 0.23013466091088475 1103 12-13 0.3036130371068708 1103 14-15 0.2488784625300582 1103 16-17 0.30408776816495475 1103 18-19 0.264467700557951 1103 20-21 0.27957338491265915 1103 22-23 0.35361999998855964 1103 24-25 0.39200430220603977 1103 26-27 0.3828711011319257 1103 28-29 0.41759794907448367 1103 30-31 0.3945559425179752 1103 32-33 0.41382488836256215 1103 34-35 0.3666987238350785 1103 36-37 0.26192637119951456 1103 38-39 0.44952261770870194 1103 40-41 0.3732060268943087 1103 42-43 0.4543856476693975 1103 44-45 0.4653462665904726 1103 46-47 0.4703774393047411 1103 48-49 0.40917878557952037 1103 50-51 0.3695125668554695 1103 52-53 0.354872989798956 1103 54-55 0.30099532144356544 1103 56-57 0.4434560606659943 1103 58-59 0.5782649792991137 1103 60-61 0.4973793757020246 1103 62-63 0.41009652262619767 1103 64-65 0.29421522245871756 1103 66-67 0.3959452501068057 1103 68-69 0.23164954487275935 1103 70-71 0.1893982387614912 1103 72-73 0.216539873464761 1103 74-75 0.0486960227232629 1103 76-77 0.09248808967024047 1103 78-79 0.002134732574553766 1103 80-81 -0.041007680649155276 1103 82-83 -0.1366592636041375 1103 84-85 -0.18515578616896988 1103 86-87 -0.3393993309086838 1103 88-89 -0.2541469860135166 1103 90-91 -0.24914824941134128 1103 92-93 -0.34232211226190046 1103 94-95 -0.7011515973088507 1103 96-97 -0.7389152391696818 1103 98-99 -1.0599076403064664 1103 100-101 -1.3270836481805475 1104 1 0.22152649531376056 1104 2 0.23998904297172885 1104 3 0.23564364933013593 1104 4 0.2761200026266337 1104 5 0.2025780950660092 1104 6 0.29367287458031655 1104 7 0.3315110142616575 1104 8 0.2620228476400328 1104 9 0.3444112493627358 1104 10-11 0.43833962562338513 1104 12-13 0.3795183723943367 1104 14-15 0.5254880216768498 1104 16-17 0.4078917895032248 1104 18-19 0.42663123769409594 1104 20-21 0.479319481072217 1104 22-23 0.5007468675426665 1104 24-25 0.48122846042090117 1104 26-27 0.4699886502582231 1104 28-29 0.5175039592908419 1104 30-31 0.5956232798175094 1104 32-33 0.443155890534463 1104 34-35 0.44383881439933504 1104 36-37 0.4675827586256531 1104 38-39 0.2752902751331092 1104 40-41 0.519375190698959 1104 42-43 0.603258978524039 1104 44-45 0.611032550992455 1104 46-47 0.6696648212292828 1104 48-49 0.6271575007302701 1104 50-51 0.6917883575545929 1104 52-53 0.6035083997398303 1104 54-55 0.7416554205546717 1104 56-57 0.6951979665007002 1104 58-59 0.7008807357515323 1104 60-61 0.5701453589466539 1104 62-63 0.6126527734131599 1104 64-65 0.5408746000063971 1104 66-67 0.48086334502673367 1104 68-69 0.5000465505965153 1104 70-71 0.555903705388765 1104 72-73 0.5477773377840478 1104 74-75 0.4958219999817217 1104 76-77 0.40422341579613885 1104 78-79 0.3826034265998608 1104 80-81 0.26173989891254124 1104 82-83 0.23426270537372318 1104 84-85 0.22922914460206556 1104 86-87 0.3513742808978222 1104 88-89 0.3519974530090728 1104 90-91 0.3630694897718847 1104 92-93 0.30169338396981615 1104 94-95 0.3582953510332274 1104 96-97 0.37070326066519144 1104 98-99 0.3523638981710526 1104 100-101 0.3420188956484935 1105 1 0.1928322073699107 1105 2 0.21999051378226397 1105 3 0.20176148576783248 1105 4 0.13655765047373336 1105 5 0.2472764359100097 1105 6 0.1575893979110461 1105 7 0.19691714182178544 1105 8 0.1579045781303421 1105 9 0.20181494191430005 1105 10-11 0.2763367902275107 1105 12-13 0.2959444662075299 1105 14-15 0.2303081946605161 1105 16-17 0.2377326968299016 1105 18-19 0.3216864543730793 1105 20-21 0.34992145114122053 1105 22-23 0.3549511386130533 1105 24-25 0.3283062458705004 1105 26-27 0.33069063544389365 1105 28-29 0.3376332271948925 1105 30-31 0.4361157153571966 1105 32-33 0.4482258970305395 1105 34-35 0.3855771490332458 1105 36-37 0.22970182890996682 1105 38-39 0.34465819430389644 1105 40-41 0.3251172758997427 1105 42-43 0.21240047458348954 1105 44-45 0.31591564198251376 1105 46-47 0.247264376717645 1105 48-49 0.2812812857794782 1105 50-51 0.21123083864854664 1105 52-53 0.4080090799897036 1105 54-55 0.39494268210972194 1105 56-57 0.36120794349885443 1105 58-59 0.4625491660289853 1105 60-61 0.4589994749690902 1105 62-63 0.4509820960640525 1105 64-65 0.4877750710743243 1105 66-67 0.48817838038747396 1105 68-69 0.4656091560556703 1105 70-71 0.3480076998650574 1105 72-73 0.46531797913915085 1105 74-75 0.29080285901678593 1105 76-77 0.22246228359887255 1105 78-79 0.39798892195659263 1105 80-81 0.426370582471737 1105 82-83 0.3192496110743406 1105 84-85 0.3788706345331363 1105 86-87 0.26902237313398913 1105 88-89 0.48137382934046613 1105 90-91 0.48877067826851217 1105 92-93 0.3693872347560898 1105 94-95 0.564316890236455 1105 96-97 0.5164922483798371 1105 98-99 0.47545909638320083 1105 100-101 0.29618496650070725 1106 1 0.17094380232636297 1106 2 0.2645959992885665 1106 3 0.2413396737309128 1106 4 0.2849896665922884 1106 5 0.23073136246661363 1106 6 0.25709706722081194 1106 7 0.20518845990316237 1106 8 0.25030026251874915 1106 9 0.26898883376403404 1106 10-11 0.33678581856496237 1106 12-13 0.1841102500369871 1106 14-15 0.21606716385328184 1106 16-17 0.20074615061906087 1106 18-19 0.3554131377215839 1106 20-21 0.44106031114382205 1106 22-23 0.3479411132653283 1106 24-25 0.34893538924759326 1106 26-27 0.2402065915078495 1106 28-29 0.22340763249841444 1106 30-31 0.31893369923864157 1106 32-33 0.1982080236391468 1106 34-35 0.1874185145603562 1106 36-37 0.30908568436297656 1106 38-39 0.3034482469491593 1106 40-41 0.3962383437536374 1106 42-43 0.30766930663860137 1106 44-45 0.34790048209690383 1106 46-47 0.349597666721543 1106 48-49 0.3842173965295146 1106 50-51 0.3413548149906802 1106 52-53 0.33351156601596443 1106 54-55 0.3823354417613629 1106 56-57 0.23620103787035873 1106 58-59 0.20730901264278856 1106 60-61 0.32200114856847506 1106 62-63 0.3074343903575638 1106 64-65 0.35622731662222407 1106 66-67 0.2690916294450574 1106 68-69 0.22586401436843317 1106 70-71 0.19939451255191187 1106 72-73 0.3099149341632028 1106 74-75 0.34154785446720126 1106 76-77 0.24862364782609347 1106 78-79 0.3233731998715861 1106 80-81 0.2960471553301822 1106 82-83 0.4494682351481707 1106 84-85 0.4548167056367376 1106 86-87 0.24033923586634032 1106 88-89 0.41609695425922055 1106 90-91 0.4571185269621303 1106 92-93 0.3581355286596377 1106 94-95 0.4343960206147237 1106 96-97 0.5266922678780794 1106 98-99 0.5153297580236575 1106 100-101 0.3189650100467887 1107 1 0.06736390173144002 1107 2 0.0900032839736582 1107 3 0.0789811879011566 1107 4 0.09673032226415046 1107 5 0.13497317911947704 1107 6 0.10421626545360851 1107 7 6.416504044537419E-4 1107 8 0.09238174455806103 1107 9 0.20292038608976526 1107 10-11 0.015602386705133142 1107 12-13 0.14617166593176023 1107 14-15 0.12252111997447912 1107 16-17 0.05683314261142414 1107 18-19 0.10044641394719633 1107 20-21 0.1366001045519667 1107 22-23 0.12910129412734506 1107 24-25 0.19595574720627695 1107 26-27 0.15917247121766565 1107 28-29 0.19308249506337205 1107 30-31 0.20072779141425912 1107 32-33 0.2525215387130686 1107 34-35 0.27884694478853334 1107 36-37 0.15696579581715042 1107 38-39 0.17324462029554866 1107 40-41 0.2823747401941361 1107 42-43 0.3054614816266792 1107 44-45 0.118952283813897 1107 46-47 0.32823561636634935 1107 48-49 0.34659062995572754 1107 50-51 0.14483289441432845 1107 52-53 0.20458894069277278 1107 54-55 0.12742009924058806 1107 56-57 0.13999218994004536 1107 58-59 0.22212093372118602 1107 60-61 0.09569967318126515 1107 62-63 0.23100575617326768 1107 64-65 0.2755811051856156 1107 66-67 0.16024769980678144 1107 68-69 0.1976329571854052 1107 70-71 0.22944274033010714 1107 72-73 0.16508137804702727 1107 74-75 0.21582851691668026 1107 76-77 0.13999700990154906 1107 78-79 0.14702253968130918 1107 80-81 0.2948944110221241 1107 82-83 0.28012385994421507 1107 84-85 0.2611976297776124 1107 86-87 0.046577390722379874 1107 88-89 0.2780625892216939 1107 90-91 0.26192938119312004 1107 92-93 0.07602616738745382 1107 94-95 0.17289367982601078 1107 96-97 0.16881595314554687 1107 98-99 0.12214942315908317 1107 100-101 0.020886668931744623 1108 1 0.16576636947143442 1108 2 0.20827660784079427 1108 3 0.1169735823003748 1108 4 0.23871587963480323 1108 5 0.2921966452021465 1108 6 0.24188787739289097 1108 7 0.16334972501233835 1108 8 0.21667233607676195 1108 9 0.2986417534361294 1108 10-11 0.331524146646494 1108 12-13 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-1.1218753836738244 2316 62-63 -1.3631620431040048 2316 64-65 -1.4268228643127756 2316 66-67 -1.6480827283172204 2316 68-69 -1.5927985744690218 2316 70-71 -1.5127130541169294 2316 72-73 -1.8610635410927152 2316 74-75 -1.4421553632416106 2316 76-77 -1.610160460865309 2316 78-79 -1.4198070994046752 2316 80-81 -1.4579128352155557 2316 82-83 -1.4426217677859086 2316 84-85 -1.3115255141585251 2316 86-87 -1.4589388579590512 2316 88-89 -1.5534456551167963 2316 90-91 -1.7755866957020139 2316 92-93 -1.77915195422489 2316 94-95 -1.7421439643888625 2316 96-97 -1.776192504241628 2316 98-99 -2.0367909305073475 2316 100-101 -1.737946991974468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6044.0 3 1576.0 4 1556.0 5 1727.0 6 1975.0 7 2308.0 8 2738.0 9 3372.0 10 3896.0 11 4706.0 12 6049.0 13 9614.0 14 7264.0 15 6599.0 16 7943.0 17 10468.0 18 9113.0 19 7472.0 20 8080.0 21 8521.0 22 9311.0 23 10266.0 24 11739.0 25 13550.0 26 15754.0 27 18315.0 28 21799.0 29 26280.0 30 31399.0 31 38670.0 32 49637.0 33 65217.0 34 91262.0 35 137052.0 36 226582.0 37 379184.0 38 376594.0 39 58529.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.07169851619372 17.75993307742965 12.942635254193045 24.225733152183587 2 15.642760198931976 18.949004452658993 40.90778392948635 24.50045141892268 3 20.177315244851233 23.332248655492513 28.335236449908592 28.155199649747665 4 12.951291975368179 15.087611959462938 35.583656655082905 36.377439410085984 5 14.331294239055698 36.954412100598475 33.51670980362918 15.197583856716642 6 34.17813144598149 33.92011477395303 17.429993092746436 14.47176068731904 7 31.188268720768207 31.044726862377246 19.43197168670851 18.335032730146036 8 27.23847892608368 35.4026180791054 18.812283467919837 18.54661952689108 9 27.286604027893514 16.68158019089705 19.368439355380904 36.66337642582853 10-11 26.158188415010986 25.713216964980084 26.74701859074632 21.381576029262607 12-13 26.701010989946877 22.66923465446902 27.440889557598748 23.188864797985353 14-15 23.28423429390586 24.31327974565566 25.16774733047259 27.23473862996589 16-17 23.48099364365181 27.866300236322733 25.7046214223364 22.948084697689055 18-19 23.308872585666833 26.566418411863236 27.474987224498683 22.64972177797125 20-21 23.933272907126497 25.914030166773372 27.27110616923971 22.88159075686042 22-23 23.54861835707644 25.857242061379537 27.16787452472382 23.4262650568202 24-25 23.62826426797723 25.959207095498467 27.264071274441797 23.148457362082507 26-27 23.39971704076583 26.32707023015063 27.162814554031577 23.110398175051962 28-29 23.015592025188596 26.360091040981754 27.527740435594716 23.096576498234935 30-31 23.23882700358299 26.14334026862807 27.347301519702004 23.27053120808694 32-33 22.95434387790018 26.280876077507493 27.403639843740528 23.3611402008518 34-35 23.66133108477515 26.24257744539597 27.00419351357377 23.091897956255107 36-37 23.613272279942514 26.00086624993984 27.05426895456466 23.33159251555299 38-39 23.30281578240021 26.181920244496816 27.297570624741045 23.21769334836193 40-41 23.370586542821197 26.091548532551744 27.37827333912733 23.159591585499722 42-43 23.342816910527958 26.04220592817133 27.125020753678562 23.48995640762215 44-45 23.25750433236438 26.31820020781091 27.025666251401297 23.39862920842341 46-47 23.26081861756589 26.386469305925488 26.721998011659743 23.63071406484888 48-49 23.547289148394903 26.296074496286238 26.728673631979127 23.42796272333974 50-51 23.40787556135401 26.422032465406808 26.765632509845787 23.40445946339339 52-53 23.544307783158285 26.439948741621055 26.664486343871957 23.351257131348703 54-55 23.149208932902244 26.120585566385625 26.95247953829543 23.777725962416703 56-57 23.25753139591096 26.69385883747531 26.72729475513387 23.32131501147986 58-59 23.43752226851434 26.20401403332082 26.836974284913467 23.52148941325137 60-61 23.520048335126713 26.138388975507787 27.170436517710517 23.171126171654986 62-63 23.368847456570062 26.241734613722656 27.03221217597384 23.35720575373344 64-65 23.241177592448352 26.070016023727238 27.006872319352826 23.681934064471587 66-67 23.08678664769613 26.536778492424514 26.735208580355867 23.641226279523494 68-69 22.9817641787284 26.366260343298876 27.060726247554573 23.591249230418153 70-71 22.968445029054365 26.342536977947677 26.946918917858902 23.74209907513905 72-73 23.116112938008516 26.21941564304131 26.883278041175156 23.78119337777502 74-75 23.072450609689614 26.4446314841262 26.705305248859663 23.77761265732452 76-77 23.168564155723804 26.266288059927582 26.81881231937221 23.7463354649764 78-79 23.143263110539657 26.32602004682839 26.889475192383927 23.641241650248023 80-81 22.979654369881185 26.735448602568724 26.822460463799946 23.462436563750146 82-83 23.22051391716676 26.577385327981208 26.650895412228216 23.55120534262382 84-85 23.24063838873904 26.254298206588334 26.581790513028714 23.92327289164391 86-87 23.232023835758508 26.047343632779157 27.0578151386291 23.662817392833237 88-89 23.179057174851263 26.091472633357988 26.892645298497932 23.836824893292818 90-91 23.097133679907238 26.27339767498997 27.074682725640674 23.55478591946212 92-93 23.17109221227764 26.104920711553458 27.010361347346528 23.71362572882237 94-95 23.13793702735984 26.038653442521166 26.901768088257356 23.92164144186164 96-97 23.23883574984284 25.948867680804465 26.87135399398457 23.940942575368123 98-99 23.077220164396326 25.97239700868565 26.732911231451347 24.217471595466684 100-101 23.404858839523094 25.401341522782513 27.258402784151343 23.935396853543054 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1256.0 1 680.5 2 122.5 3 145.5 4 141.5 5 145.5 6 211.5 7 369.5 8 591.0 9 788.5 10 840.0 11 867.5 12 1003.0 13 1198.0 14 1364.0 15 1468.5 16 1576.5 17 1688.5 18 1731.5 19 1731.5 20 1762.5 21 1817.0 22 2015.0 23 2417.5 24 2882.0 25 3556.5 26 4675.5 27 6148.5 28 7629.5 29 9460.0 30 12033.5 31 15105.0 32 18303.0 33 21444.0 34 25038.5 35 28365.5 36 32442.0 37 36958.5 38 40154.5 39 42307.0 40 44337.5 41 49008.5 42 54034.0 43 57791.5 44 61960.5 45 65978.5 46 67505.5 47 68393.0 48 75212.0 49 76745.5 50 72033.0 51 69609.5 52 67067.5 53 66328.5 54 64643.0 55 59719.0 56 53612.0 57 47911.0 58 42709.0 59 37447.5 60 32135.0 61 26684.5 62 21909.0 63 17552.0 64 13710.5 65 10778.5 66 8125.5 67 6867.0 68 5466.5 69 3679.0 70 2787.5 71 2098.0 72 1642.0 73 1346.0 74 1034.5 75 840.5 76 575.5 77 325.5 78 217.0 79 153.5 80 90.0 81 71.0 82 62.5 83 42.0 84 29.5 85 19.5 86 15.0 87 17.0 88 15.5 89 7.5 90 4.0 91 4.5 92 5.0 93 4.5 94 4.5 95 3.0 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3930430609964301 2 0.11553137637167654 3 0.11647690170259563 4 0.12835506367226673 5 0.1308961629991118 6 0.07120987648484411 7 0.08184703645768389 8 0.07818312580037241 9 0.07812403046718998 10-11 0.07599659847262202 12-13 0.08054693912767015 14-15 0.07357368981214185 16-17 0.07522835914125026 18-19 0.0853927564486305 20-21 0.08353125345338354 22-23 0.09366610309417255 24-25 0.10716938672636082 26-27 0.10823310272364481 28-29 0.10244176007176536 30-31 0.09180460009892559 32-33 0.09050450276891184 34-35 0.3408027864631502 36-37 0.5356400999656656 38-39 0.5155181390170437 40-41 0.4878024277544778 42-43 0.516759141013875 44-45 0.5271303719873938 46-47 0.5240278669953155 48-49 0.5308533779778877 50-51 0.5303215199792457 52-53 0.5285486599837724 54-55 0.5232005323307612 56-57 0.4942142714047729 58-59 0.48393168343102777 60-61 0.43009583490182196 62-63 0.5069493157055894 64-65 0.5349309559674763 66-67 0.544888519608718 68-69 0.5583918032409063 70-71 0.554757440250186 72-73 0.5408109416191293 74-75 0.5457158542732722 76-77 0.5209844573364197 78-79 0.4948347724031885 80-81 0.5024580703837237 82-83 0.5164045690147804 84-85 0.5099927253644853 86-87 0.5117655853599585 88-89 0.5221663640000686 90-91 0.5398063209550279 92-93 0.5462477122719142 94-95 0.5406336556195821 96-97 0.5421996819489169 98-99 0.5421996819489169 100-101 0.5197730030061796 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1692181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.032646435695014 #Duplication Level Percentage of deduplicated Percentage of total 1 80.45107623307028 47.49239938637985 2 11.511113054289178 13.590629340303328 3 3.3765736657389747 5.9798423812094255 4 1.4898457036484298 3.5179813866886804 5 0.7773970118216263 2.294590146951594 6 0.49742948345015753 1.7618747293922135 7 0.3181047821492556 1.3144996993884557 8 0.23275246740370353 1.099199529222277 9 0.19133446461324907 1.0165481820429239 >10 1.0087778148735456 10.950950595856396 >50 0.09086390869329083 3.727931610871263 >100 0.05081516594590581 5.349408085369903 >500 0.0030124956172310003 1.1612071154272887 >1k 9.037486851693E-4 0.7429378108964322 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.137455744982363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1926 0.1138176117093857 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.9095333182443247E-5 2 0.0 0.0 0.0 0.0 5.9095333182443247E-5 3 0.0 0.0 0.0 0.0 5.9095333182443247E-5 4 0.0 0.0 0.0 0.0 1.1819066636488649E-4 5 0.0 0.0 0.0 0.0 1.1819066636488649E-4 6 0.0 0.0 0.0 0.0 1.1819066636488649E-4 7 0.0 0.0 0.0 0.0 1.1819066636488649E-4 8 0.0 0.0 0.0 0.0 1.1819066636488649E-4 9 0.0 0.0 0.0 5.9095333182443247E-5 1.1819066636488649E-4 10-11 0.0 0.0 0.0 1.1819066636488649E-4 1.1819066636488649E-4 12-13 0.0 0.0 0.0 1.1819066636488649E-4 1.1819066636488649E-4 14-15 0.0 0.0 0.0 1.1819066636488649E-4 1.1819066636488649E-4 16-17 0.0 0.0 0.0 1.4773833295610812E-4 1.1819066636488649E-4 18-19 0.0 0.0 0.0 1.7728599954732976E-4 1.1819066636488649E-4 20-21 0.0 0.0 0.0 1.7728599954732976E-4 1.1819066636488649E-4 22-23 0.0 0.0 0.0 2.0683366613855137E-4 1.4773833295610812E-4 24-25 0.0 0.0 0.0 3.545719990946595E-4 1.7728599954732976E-4 26-27 0.0 0.0 0.0 5.318579986419893E-4 1.7728599954732976E-4 28-29 0.0 0.0 0.0 0.0016546693291084112 1.7728599954732976E-4 30-31 0.0 0.0 0.0 0.0052299369866462275 2.0683366613855137E-4 32-33 0.0 0.0 0.0 0.013769212631509278 2.3638133272977299E-4 34-35 0.0 0.0 0.0 0.025972398933683808 2.3638133272977299E-4 36-37 0.0 0.0 0.0 0.04996510420575577 2.3638133272977299E-4 38-39 0.0 0.0 0.0 0.08935214377185419 2.3638133272977299E-4 40-41 0.0 0.0 0.0 0.13095525833229424 2.3638133272977299E-4 42-43 0.0 0.0 0.0 0.17335615989069725 2.3638133272977299E-4 44-45 0.0 0.0 0.0 0.22716246075331187 2.3638133272977299E-4 46-47 0.0 0.0 0.0 0.29432430691515865 2.3638133272977299E-4 48-49 0.0 0.0 0.0 0.36931628472367906 2.3638133272977299E-4 50-51 0.0 0.0 0.0 0.45219748951205574 2.3638133272977299E-4 52-53 0.0 0.0 0.0 0.5369401972956793 2.9547666591221624E-4 54-55 0.0 0.0 0.0 0.6306949433896256 2.9547666591221624E-4 56-57 0.0 0.0 0.0 0.7466399870935791 2.9547666591221624E-4 58-59 0.0 0.0 0.0 0.883268397411388 2.9547666591221624E-4 60-61 0.0 0.0 0.0 1.0309180873677224 2.9547666591221624E-4 62-63 0.0 0.0 0.0 1.1826749029802368 2.9547666591221624E-4 64-65 0.0 0.0 0.0 1.3430891849039788 2.9547666591221624E-4 66-67 0.0 0.0 0.0 1.53346480075122 2.9547666591221624E-4 68-69 0.0 0.0 0.0 1.752531200858537 2.9547666591221624E-4 70-71 0.0 0.0 0.0 2.002268078887542 2.9547666591221624E-4 72-73 0.0 0.0 0.0 2.256466654571822 4.1366733227710275E-4 74-75 0.0 0.0 0.0 2.5185840049025487 4.1366733227710275E-4 76-77 0.0 0.0 0.0 2.8063782775010475 4.4321499886832436E-4 78-79 0.0 0.0 0.0 3.132939088667229 4.7276266545954597E-4 80-81 0.0 0.0 0.0 3.4968481504047144 5.023103320507676E-4 82-83 0.0 0.0 0.0 3.868646439122056 5.318579986419893E-4 84-85 0.0 0.0 0.0 4.245231449827176 5.318579986419893E-4 86-87 0.0 0.0 0.0 4.649000313796219 5.318579986419893E-4 88-89 0.0 0.0 0.0 5.097238415985052 5.318579986419893E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1840 0.0 32.694473 2 CACGTTA 60 0.004018293 31.558372 7 GTACCTG 2220 0.0 29.091633 1 GTACATG 7685 0.0 28.980848 1 TACATGG 7680 0.0 28.186647 2 ACATGGG 7800 0.0 26.17406 3 CATGGGG 3965 0.0 24.132187 4 ACCTGGG 2755 0.0 21.492002 3 GGTATCA 5400 0.0 21.369514 1 GTATCAA 6755 0.0 20.87917 1 CCCTATA 210 8.845109E-7 20.30073 2 ATGGGGA 2170 0.0 20.30013 5 GTACCGT 165 6.493855E-5 20.0826 6 ATTCGCG 270 0.0 19.37332 80-81 ACCGTAT 150 7.9030887E-4 18.935022 8 CATGGGA 3840 0.0 18.74998 4 GCACCGT 515 0.0 18.383518 6 AGTACAT 5440 0.0 18.37266 2 CGCGGAA 290 0.0 18.038301 82-83 TCTACGG 160 0.0012137074 17.76314 2 >>END_MODULE