FastQCFastQC Report
Fri 27 May 2016
SRR939102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692181
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24950.1474428562901959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21290.12581396434542166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18350.032.327542
GTACCTG21550.028.3149851
GTACATG77700.027.6700021
TACATGG77850.027.0049782
ACATGGG78350.025.9240883
GTATCAA70900.024.205271
GGTATCA54000.023.8354681
CCGTATA1000.001662743223.7284169
ACCTGGG28350.020.7571323
TCAACGC82450.020.5483194
ATCAACG82400.020.5031933
CATGGGG41050.020.34694
CAACGCA83350.020.3264395
CATGGGA38400.020.2680234
TATCAAC84950.019.9994662
AACGCAG85750.019.812886
TATACCG1200.004789536419.773685
GTGTAGC9050.018.9630241
GAGTACT33900.018.89873912-13
TGCACCG4550.018.774135
Produced by FastQC (version 0.11.5)