##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939102_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1692181 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52320939663074 34.0 31.0 34.0 31.0 34.0 2 32.73846946632777 34.0 31.0 34.0 31.0 34.0 3 32.85262333048297 34.0 31.0 34.0 31.0 34.0 4 36.28775586063193 37.0 37.0 37.0 35.0 37.0 5 36.24898695825092 37.0 37.0 37.0 35.0 37.0 6 36.286195152882584 37.0 37.0 37.0 35.0 37.0 7 36.23916590482933 37.0 37.0 37.0 35.0 37.0 8 36.212596052077174 37.0 37.0 37.0 35.0 37.0 9 37.96914869035877 39.0 38.0 39.0 35.0 39.0 10-11 37.87891898088916 39.0 38.0 39.0 35.0 39.0 12-13 37.79033625835534 39.0 38.0 39.0 35.0 39.0 14-15 39.23193884105778 40.5 39.0 41.0 36.0 41.0 16-17 39.32518920848301 41.0 39.0 41.0 36.0 41.0 18-19 39.33388745057414 41.0 39.0 41.0 36.0 41.0 20-21 39.292846332632266 41.0 39.0 41.0 36.0 41.0 22-23 39.227634041512104 41.0 39.0 41.0 36.0 41.0 24-25 39.15517075301047 41.0 39.0 41.0 36.0 41.0 26-27 39.01977300300618 40.0 39.0 41.0 35.0 41.0 28-29 38.87275533763823 40.0 38.0 41.0 35.0 41.0 30-31 38.71555702374627 40.0 38.0 41.0 35.0 41.0 32-33 38.672861827428626 40.0 38.0 41.0 35.0 41.0 34-35 38.59358514248771 40.0 38.0 41.0 34.5 41.0 36-37 38.46643030503238 40.0 38.0 41.0 34.0 41.0 38-39 38.353122686048366 40.0 38.0 41.0 34.0 41.0 40-41 38.182114383745 40.0 38.0 41.0 33.5 41.0 42-43 38.02155384087163 40.0 38.0 41.0 33.0 41.0 44-45 37.80222358010165 40.0 37.5 41.0 33.0 41.0 46-47 37.54761547375843 40.0 37.0 41.0 33.0 41.0 48-49 37.26150571363229 40.0 37.0 41.0 32.0 41.0 50-51 37.018126902500384 40.0 36.0 41.0 31.0 41.0 52-53 36.74633771446435 39.0 36.0 41.0 31.0 41.0 54-55 36.486525968557736 39.0 35.0 40.0 31.0 41.0 56-57 36.225031778515415 39.0 35.0 40.0 30.0 41.0 58-59 35.87430274893761 38.0 35.0 40.0 29.5 41.0 60-61 35.48224037499534 38.0 34.5 40.0 28.5 41.0 62-63 35.06692901054911 37.5 34.0 40.0 28.0 41.0 64-65 34.58668694424533 37.0 34.0 39.5 27.5 41.0 66-67 34.14298027220492 36.0 33.5 39.0 26.0 40.5 68-69 33.63527926386125 36.0 33.0 39.0 26.0 40.0 70-71 33.11325591056749 35.0 32.5 38.0 25.5 40.0 72-73 32.51596076306258 35.0 32.0 37.0 24.0 39.0 74-75 31.947857528243137 35.0 31.0 37.0 22.5 39.0 76-77 29.940288893445796 32.5 28.5 35.0 20.0 37.0 78-79 30.94576821273847 34.0 30.5 35.5 20.0 37.0 80-81 30.95446911411959 34.0 31.0 35.0 20.0 37.0 82-83 30.562369510117417 34.0 30.5 35.0 19.0 37.0 84-85 30.081202601849327 34.0 30.0 35.0 16.5 36.0 86-87 29.35860673296769 34.0 29.0 35.0 7.5 36.0 88-89 28.67788108955248 33.5 29.0 35.0 2.0 35.0 90-91 28.098904017950797 33.0 27.5 35.0 2.0 35.0 92-93 27.612445713549555 33.0 27.0 34.5 2.0 35.0 94-95 27.025649147461177 32.5 25.5 34.0 2.0 35.0 96-97 26.173740870509715 32.0 24.5 34.0 2.0 35.0 98-99 24.88858047691116 32.0 12.5 34.0 2.0 35.0 100-101 18.892753493863836 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06956828729009601 1101 2 0.09357350456065205 1101 3 0.18281833891160204 1101 4 0.11897308940655194 1101 5 0.08993738127029616 1101 6 0.059409098809808825 1101 7 0.08642709569708273 1101 8 0.14146374358164593 1101 9 0.14222887661636463 1101 10-11 0.1264010124439352 1101 12-13 0.15577486408164276 1101 14-15 0.22542659004485444 1101 16-17 0.14698976933866703 1101 18-19 0.15383767303516294 1101 20-21 0.1090748007204212 1101 22-23 0.18140902406530302 1101 24-25 0.15259602296318775 1101 26-27 0.16358124902029658 1101 28-29 0.13498372490987265 1101 30-31 0.216217248719353 1101 32-33 0.2362434303850378 1101 34-35 0.2776265022133302 1101 36-37 0.28991746940096874 1101 38-39 0.18553099064298806 1101 40-41 0.3676385473945274 1101 42-43 0.3668324057215031 1101 44-45 0.47160591823099907 1101 46-47 0.39934479876066575 1101 48-49 0.3446516893484599 1101 50-51 0.35731880635668745 1101 52-53 0.39690222866271085 1101 54-55 0.4204482364863651 1101 56-57 0.37841747297628814 1101 58-59 0.40525691211080783 1101 60-61 0.4192750981671409 1101 62-63 0.20036006763733383 1101 64-65 0.11748863421910016 1101 66-67 0.14233534173939688 1101 68-69 0.26426371327842446 1101 70-71 0.1371722847349517 1101 72-73 0.26155896685811086 1101 74-75 0.08445488022700331 1101 76-77 0.2813265458260652 1101 78-79 0.3225903410069222 1101 80-81 0.3300697005871456 1101 82-83 0.2450610464858194 1101 84-85 0.4241570993945807 1101 86-87 0.7219281101011958 1101 88-89 0.5385179243500176 1101 90-91 0.590207232447284 1101 92-93 0.49222635855115016 1101 94-95 0.1824140712597746 1101 96-97 0.05599337463163323 1101 98-99 0.22512625267900077 1101 100-101 -0.5307996916374123 1102 1 0.08827198757273891 1102 2 0.05343245784066397 1102 3 0.08088598961083449 1102 4 -0.0168815964481297 1102 5 0.029757909248715464 1102 6 0.06070041062743314 1102 7 0.15615793384897358 1102 8 0.13853612354876077 1102 9 0.1387572060394291 1102 10-11 0.15249719577696652 1102 12-13 0.20989149780354666 1102 14-15 0.10031644059260003 1102 16-17 0.11052683682309805 1102 18-19 0.05441520716532722 1102 20-21 0.13436876351438087 1102 22-23 0.09001938596514236 1102 24-25 0.11167307677707328 1102 26-27 0.07270056142908032 1102 28-29 0.14917707068216401 1102 30-31 0.08704103730630663 1102 32-33 0.19852682622106244 1102 34-35 0.1479942212652574 1102 36-37 0.1223993189880801 1102 38-39 0.07586393294960914 1102 40-41 0.05928751009611943 1102 42-43 0.08714516550794826 1102 44-45 -0.06026237350571506 1102 46-47 0.1174347102716311 1102 48-49 0.008578782880817926 1102 50-51 -0.11748952897796983 1102 52-53 0.041529614079571786 1102 54-55 0.09199873435265715 1102 56-57 0.07264005338308266 1102 58-59 -0.11099602058948932 1102 60-61 -0.17104583623274294 1102 62-63 -0.19487625720425683 1102 64-65 -0.008825307884315237 1102 66-67 -0.034634185888023694 1102 68-69 -0.13440502552190026 1102 70-71 -0.20005430446532557 1102 72-73 -0.18436088498280157 1102 74-75 -0.2432081040149292 1102 76-77 -0.04335339727493093 1102 78-79 0.05627829245803184 1102 80-81 -0.028102603637787382 1102 82-83 -0.29242047981149355 1102 84-85 -0.13380529738360636 1102 86-87 0.13420111447946326 1102 88-89 -0.23019478186268927 1102 90-91 -0.06198698158903326 1102 92-93 -0.009068878928299995 1102 94-95 -0.08098744543121583 1102 96-97 -0.016341100992313784 1102 98-99 -0.2179939095201071 1102 100-101 -1.0005640133754206 1103 1 0.08916604091226787 1103 2 0.06886849864022082 1103 3 0.08597164367203192 1103 4 0.049059526432849054 1103 5 0.11846345220079257 1103 6 0.10970096438674659 1103 7 0.10688050043406605 1103 8 0.055788852153106916 1103 9 0.10370379506071004 1103 10-11 0.11839656341955873 1103 12-13 0.11958384877976158 1103 14-15 0.013259448041551991 1103 16-17 0.13615204894863808 1103 18-19 0.17767957364680598 1103 20-21 0.14391999518227294 1103 22-23 0.16895487300539003 1103 24-25 0.1655699346395707 1103 26-27 0.14817862541507765 1103 28-29 0.19813475275287118 1103 30-31 0.18674562457698585 1103 32-33 0.24845023773568897 1103 34-35 0.3016423340178065 1103 36-37 0.2558852338520481 1103 38-39 0.2936969923804966 1103 40-41 0.3038588825260504 1103 42-43 0.30047200854397715 1103 44-45 0.27352833226945705 1103 46-47 0.3516271148374628 1103 48-49 0.40973877466374375 1103 50-51 0.344923611194595 1103 52-53 0.3039208718277351 1103 54-55 0.21597472620703684 1103 56-57 0.23631455901892195 1103 58-59 0.17535850148049548 1103 60-61 0.1505550194147034 1103 62-63 0.2542075837417812 1103 64-65 0.15180955037116206 1103 66-67 0.08620435754635025 1103 68-69 0.15412076894408244 1103 70-71 0.10743908614934838 1103 72-73 0.08075071538161183 1103 74-75 -0.11003836785444321 1103 76-77 -0.0840681040504414 1103 78-79 -0.03208450027521792 1103 80-81 0.1913017478386081 1103 82-83 -0.06264988800355908 1103 84-85 -0.21484283690594808 1103 86-87 -0.26577834283641266 1103 88-89 0.013509555323143019 1103 90-91 0.27360321865747395 1103 92-93 0.34311288809979956 1103 94-95 0.2579277668729567 1103 96-97 -0.18683354485720116 1103 98-99 -0.3348788794206108 1103 100-101 -1.2745911386500666 1104 1 0.10467456718892265 1104 2 0.04843748531843062 1104 3 0.1418588932392879 1104 4 0.03950156670219229 1104 5 0.12120556280348893 1104 6 0.09187597361826505 1104 7 0.0968544995615801 1104 8 0.07234114409207137 1104 9 0.08701795877420437 1104 10-11 0.2039298102387619 1104 12-13 0.17812672308655664 1104 14-15 0.07398524249263971 1104 16-17 0.10574336508965843 1104 18-19 0.1937220653384415 1104 20-21 0.19252822990829088 1104 22-23 0.28020388573110466 1104 24-25 0.09074143313856808 1104 26-27 0.19931953929084045 1104 28-29 0.13768494995336056 1104 30-31 0.16049313757847727 1104 32-33 0.20972661645388513 1104 34-35 0.21174133913361004 1104 36-37 0.2560353122616661 1104 38-39 0.19715248416921582 1104 40-41 0.2594426684616735 1104 42-43 0.3009733297851156 1104 44-45 0.3085735486897363 1104 46-47 0.22490094815800887 1104 48-49 0.21188413281061003 1104 50-51 0.23645444064914045 1104 52-53 0.12899515852074472 1104 54-55 0.14402272755688728 1104 56-57 0.26783166096243605 1104 58-59 0.17602940284549362 1104 60-61 0.32187863891591206 1104 62-63 0.17741258872955967 1104 64-65 0.019926664100495373 1104 66-67 0.08199007949345827 1104 68-69 0.10517400989581915 1104 70-71 0.05585493222486093 1104 72-73 -0.16297005059484349 1104 74-75 -0.26212312230454415 1104 76-77 -0.240313506839275 1104 78-79 0.14264407613779895 1104 80-81 0.16227281026232276 1104 82-83 0.04478653958763701 1104 84-85 -0.007163782552549236 1104 86-87 0.03278908667230951 1104 88-89 -0.11169908122298366 1104 90-91 0.18150806894578508 1104 92-93 -0.0633532868127702 1104 94-95 -0.0860116726732123 1104 96-97 -0.4579402289849668 1104 98-99 -0.4380632973813512 1104 100-101 -0.5821944330831315 1105 1 0.061322426546745135 1105 2 0.06765607636364024 1105 3 0.061536704019509614 1105 4 0.1154905846501677 1105 5 0.049059854506076306 1105 6 0.06111356263293999 1105 7 0.01732164533115821 1105 8 0.05683961812614058 1105 9 0.12155545014341129 1105 10-11 0.0615112257304844 1105 12-13 0.058787607288827815 1105 14-15 0.11403868194223321 1105 16-17 0.15158812103724983 1105 18-19 0.1640833944609028 1105 20-21 0.06546490742898925 1105 22-23 0.1281132919730652 1105 24-25 0.04397639920778573 1105 26-27 0.1329755657327425 1105 28-29 0.16580643890389268 1105 30-31 0.23606195312658684 1105 32-33 0.2632647329175697 1105 34-35 0.2988678656102408 1105 36-37 0.3778090280602129 1105 38-39 0.3054494898440865 1105 40-41 0.35550028337895867 1105 42-43 0.3138623285308455 1105 44-45 0.28720491862718234 1105 46-47 0.34120386158854643 1105 48-49 0.30482383366408783 1105 50-51 0.27529752559252785 1105 52-53 0.3296557975879182 1105 54-55 0.2323154842235482 1105 56-57 0.40481641764543497 1105 58-59 0.29732081535473043 1105 60-61 0.22704118390841188 1105 62-63 0.2985757138751026 1105 64-65 0.22705866526079888 1105 66-67 0.18623214222352402 1105 68-69 -0.05796114533598029 1105 70-71 -0.010006533764688186 1105 72-73 -0.13581337250216308 1105 74-75 0.23896180474733697 1105 76-77 -0.12271301166639148 1105 78-79 0.07586212788581648 1105 80-81 0.4082762911805986 1105 82-83 0.13767721342314232 1105 84-85 0.003815205284663392 1105 86-87 -0.029381257701636798 1105 88-89 0.13252900880695861 1105 90-91 -0.23468882355839327 1105 92-93 -0.17924662036400463 1105 94-95 -0.226681626873809 1105 96-97 -0.5011211836227183 1105 98-99 -0.44922777076989107 1105 100-101 -1.1779288782402837 1106 1 0.02573406699910663 1106 2 0.015132678469456096 1106 3 0.05379346341116076 1106 4 0.07647540851601775 1106 5 0.08581485905566666 1106 6 0.050092991984300284 1106 7 0.06231140875244989 1106 8 0.05274254936232836 1106 9 0.04707215364009443 1106 10-11 0.018727201292684015 1106 12-13 0.0723311696182165 1106 14-15 0.06189428118830165 1106 16-17 0.043810758499880365 1106 18-19 0.09586756513554207 1106 20-21 0.07263579329926984 1106 22-23 0.11041375993094249 1106 24-25 0.14023761069875462 1106 26-27 0.1618983689304585 1106 28-29 0.14356137845933148 1106 30-31 -0.10039001619617949 1106 32-33 -0.014850517959850151 1106 34-35 -0.07080748860787622 1106 36-37 -0.05825257014594598 1106 38-39 -0.08779436820868369 1106 40-41 -0.06373717274998825 1106 42-43 -0.08715158021737324 1106 44-45 -0.06787234316925606 1106 46-47 -0.15665864328601486 1106 48-49 -0.13254780288862378 1106 50-51 -0.045093982921699194 1106 52-53 -0.07071873120448657 1106 54-55 -0.19464977640299708 1106 56-57 -0.19030292786347047 1106 58-59 -0.260116952746138 1106 60-61 -0.20598275018751622 1106 62-63 -0.1855839930012806 1106 64-65 -0.2042283001623133 1106 66-67 -0.331481607289021 1106 68-69 -0.3945418843194659 1106 70-71 -0.43965889630205623 1106 72-73 -0.4187548452696106 1106 74-75 -0.3506939699829381 1106 76-77 -0.5023905594370923 1106 78-79 -0.4505336214967386 1106 80-81 -0.26307916182863167 1106 82-83 -0.2673049944281516 1106 84-85 -0.535246279181731 1106 86-87 -0.6587094619132543 1106 88-89 -0.7972021591379246 1106 90-91 -0.4676026738118715 1106 92-93 -0.33936052261280025 1106 94-95 -0.5652073158118007 1106 96-97 -0.9004058417534857 1106 98-99 -0.8522999630225918 1106 100-101 -1.48692122517906 1107 1 0.003186717801128225 1107 2 -0.017837612968158112 1107 3 0.028301301601793227 1107 4 0.026266902604426434 1107 5 -0.004871092446705916 1107 6 0.013049069249156275 1107 7 0.057155241119346556 1107 8 0.07644460398491759 1107 9 0.055820302346070605 1107 10-11 0.04268576996000206 1107 12-13 0.04549328963226884 1107 14-15 -0.03550143447804288 1107 16-17 -0.06405928065969846 1107 18-19 0.04381757078355264 1107 20-21 0.11261707640291263 1107 22-23 -0.006523697708942677 1107 24-25 -0.027519436769679828 1107 26-27 -0.06894740572627 1107 28-29 -0.022602457923930785 1107 30-31 -0.09875522944015103 1107 32-33 0.0242561071625218 1107 34-35 -0.04754864632557343 1107 36-37 0.009327113941708376 1107 38-39 0.05635868046466186 1107 40-41 0.011220624021852643 1107 42-43 -0.04799828052110655 1107 44-45 -0.0625416361632034 1107 46-47 0.00858111497891656 1107 48-49 -0.18172946817465885 1107 50-51 -0.23917431339337725 1107 52-53 -0.13741473617474043 1107 54-55 -0.20434945885901357 1107 56-57 -0.32604474765411595 1107 58-59 -0.36396591853171145 1107 60-61 -0.4039657025935739 1107 62-63 -0.33208371373827816 1107 64-65 -0.552939532810349 1107 66-67 -0.4707761858215349 1107 68-69 -0.45818207748160233 1107 70-71 -0.5001926808490893 1107 72-73 -0.7451230770641786 1107 74-75 -0.6738628362379266 1107 76-77 -0.4924159284407814 1107 78-79 -0.5742738002250576 1107 80-81 -0.47383329743121294 1107 82-83 -0.7400356688012231 1107 84-85 -0.5174299275488288 1107 86-87 -0.4463397584968334 1107 88-89 -0.566476404814054 1107 90-91 -0.3227271498015263 1107 92-93 -0.3076963347974555 1107 94-95 -0.2555058291285697 1107 96-97 -0.46717676137594566 1107 98-99 -0.42740641242891897 1107 100-101 -0.916243761389115 1108 1 -0.01870646252995556 1108 2 0.009570592356361374 1108 3 -0.042069052282691644 1108 4 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2316 48-49 -0.6760048023018044 2316 50-51 -0.5395854496361849 2316 52-53 -0.712815865585398 2316 54-55 -0.7400648673595072 2316 56-57 -0.44916120360188216 2316 58-59 -0.36053791186461126 2316 60-61 -0.4148964474555612 2316 62-63 -0.49972116943734335 2316 64-65 -0.45820051604546563 2316 66-67 -0.3779226078106319 2316 68-69 -0.29139569373460716 2316 70-71 -0.2545214427583389 2316 72-73 -0.039388362254683784 2316 74-75 -0.13156804085729235 2316 76-77 -0.055469918177394106 2316 78-79 -0.09625556842247462 2316 80-81 -0.1423621050378614 2316 82-83 -0.12315909725139207 2316 84-85 -0.23129179003783307 2316 86-87 -0.3319842630477652 2316 88-89 -0.5176830631608773 2316 90-91 -0.7676104165255673 2316 92-93 -0.8208737748785637 2316 94-95 -0.6763753652999682 2316 96-97 -0.5757100488996016 2316 98-99 -0.638255699150907 2316 100-101 0.6049328287428288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 11.0 5 36.0 6 52.0 7 116.0 8 324.0 9 615.0 10 1086.0 11 1635.0 12 2472.0 13 3348.0 14 4262.0 15 5699.0 16 7419.0 17 10345.0 18 10466.0 19 8256.0 20 8558.0 21 9459.0 22 10158.0 23 11436.0 24 13063.0 25 15655.0 26 18364.0 27 21939.0 28 26532.0 29 32615.0 30 39652.0 31 48434.0 32 61581.0 33 81518.0 34 113882.0 35 169493.0 36 276668.0 37 417246.0 38 250747.0 39 9032.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56777132655926 18.215107483710227 13.256096757595017 23.961024432135495 2 16.0327411783964 19.437932467035147 40.25213614855621 24.277190206012243 3 20.24015161498681 23.457065172106294 28.532822434479527 27.769960778427365 4 13.24007301819368 15.562992374929161 35.39993653161216 35.796998075264995 5 14.336823306726645 37.1050141799252 33.488202503160124 15.069960010188035 6 34.150247520803035 34.294676515100925 17.14320158422769 14.411874379868348 7 31.114697541220472 31.264858782837063 19.279497878773018 18.340945797169454 8 27.192008419903075 35.731047683433395 18.601733502503574 18.475210394159962 9 27.000835017057867 16.83602404234535 19.218688780928282 36.9444521596685 10-11 26.089673622384367 25.945215080419885 26.630218635004177 21.334892662191574 12-13 26.597332082088144 22.8353527193604 27.410365676012198 23.156949522539254 14-15 23.173335523815883 24.554314610420118 25.062892654338896 27.209457211425097 16-17 23.376167489044242 28.112636819566156 25.628779777637455 22.882415913752148 18-19 23.122556038627074 26.762887657998764 27.568179763275914 22.54637654009825 20-21 23.776209518958076 26.02097529755978 27.3958873193825 22.806927864099645 22-23 23.5213313469422 25.907069042850615 27.265375276049074 23.306224334158106 24-25 23.504148494840972 26.068651089127638 27.37980002127433 23.04740039475706 26-27 23.24375195759191 26.43080023402457 27.321866523257672 23.003581285125847 28-29 22.962421255422 26.43208760297368 27.612783510028482 22.992707631575836 30-31 23.160436147197018 26.185851277138795 27.476670639842897 23.177041935821286 32-33 22.91298120974683 26.319325863877996 27.48247010292342 23.285222823451754 34-35 23.608688739780483 26.303780829949236 27.10730545165052 22.980224978619763 36-37 23.538791239678165 26.045113866391528 27.18326056315341 23.232834330776893 38-39 23.267533023295403 26.233393780484633 27.457271272156405 23.041801924063563 40-41 23.309227173639705 26.13069098381559 27.47726916108732 23.082812681457384 42-43 23.335676691096097 26.150129150631805 27.13942210374426 23.37477205452784 44-45 23.276675425924193 26.388521580543163 27.08392369428901 23.25087929924364 46-47 23.279061816576476 26.459262971546533 26.787823378170692 23.4738518337063 48-49 23.53459843161381 26.40572795747823 26.77242739365938 23.287246217248583 50-51 23.43138404951989 26.44254989972925 26.757707639635175 23.36835841111568 52-53 23.61103806271315 26.47479790873435 26.692357377845514 23.221806650706984 54-55 23.12831783361236 26.173293518837525 26.953795131844643 23.74459351570547 56-57 23.316070596487787 26.67185917814323 26.704923026828403 23.30714719854058 58-59 23.44038140770409 26.213949392859092 26.87426221927397 23.47140698016284 60-61 23.448083898792884 26.24863042761389 27.17494595581674 23.128339717776488 62-63 23.381549842538437 26.286123230736592 26.979468241459582 23.352858685265392 64-65 23.20949146372237 26.161952000170203 26.898537997353632 23.730018538753797 66-67 23.103271087824407 26.591864106087932 26.724772455763947 23.580092350323717 68-69 22.954505563215346 26.418993710171463 26.986012031225158 23.640488695388033 70-71 22.918520168811877 26.42733823450763 26.92548278713931 23.728658809541187 72-73 23.048332300149923 26.26465490393758 26.820062392852773 23.866950403059718 74-75 22.950559071399574 26.511939325639517 26.659618563262438 23.87788303969847 76-77 23.03231313341035 26.316212879706086 26.78990459323617 23.861569393647397 78-79 22.97064832215122 26.320819592727407 26.895080629122 23.813451455999378 80-81 22.75235879998156 26.756881975494245 26.79753973291163 23.693219491612567 82-83 23.012786457240686 26.50354778832761 26.713779436124153 23.769886318307556 84-85 22.893798003877837 26.265275404935995 26.60717145506302 24.233755136123143 86-87 22.95314908786306 26.03769700655338 27.059569599615717 23.949584305967843 88-89 22.837696505950994 26.08563557631114 26.852952651652824 24.22371526608504 90-91 22.724896344108483 26.24246132227354 27.002100118506263 24.030542215111712 92-93 22.770436495859485 26.099483447692652 26.872539048718785 24.257541007729078 94-95 22.65322090249211 25.93732585344003 26.886130975350746 24.523322268717116 96-97 22.647813679505916 25.891940637555912 26.910507800288503 24.54973788264967 98-99 22.470744803442663 25.86237972600991 26.79463980739615 24.872235663151276 100-101 22.892795166461653 24.734712956952183 27.579806243615838 24.79268563297032 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 105.0 1 76.5 2 75.5 3 105.5 4 92.5 5 84.5 6 133.5 7 271.0 8 472.5 9 596.0 10 588.5 11 567.0 12 644.0 13 754.0 14 852.0 15 941.0 16 1002.0 17 1070.0 18 1157.5 19 1243.0 20 1410.0 21 1657.5 22 1890.5 23 2278.5 24 2841.5 25 3544.0 26 4725.0 27 6361.5 28 7954.5 29 9813.5 30 12225.5 31 15114.5 32 18436.0 33 21658.5 34 25226.0 35 28670.5 36 32830.0 37 37336.0 38 40440.0 39 42501.0 40 44467.5 41 49295.0 42 54694.0 43 58416.0 44 62060.5 45 66152.5 46 67880.5 47 68716.0 48 75370.5 49 77451.5 50 73400.5 51 70524.0 52 67496.0 53 66653.5 54 65328.5 55 60108.0 56 53484.5 57 47831.0 58 42769.5 59 37567.5 60 32001.0 61 26307.5 62 21576.0 63 17451.5 64 13681.0 65 10697.5 66 7897.0 67 6545.5 68 5159.0 69 3338.0 70 2461.0 71 1864.5 72 1527.5 73 1254.0 74 979.5 75 840.0 76 568.5 77 288.5 78 158.5 79 93.5 80 46.5 81 27.5 82 17.5 83 13.0 84 8.5 85 5.0 86 3.0 87 1.0 88 3.0 89 4.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4271410682426998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.09143998189319E-4 16-17 0.0020387889947942922 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.9095333182443247E-5 26-27 0.0030138619923046057 28-29 5.9095333182443247E-5 30-31 0.0 32-33 2.9547666591221623E-5 34-35 0.011700875970123764 36-37 0.02597239893368381 38-39 0.02038788994794292 40-41 0.008952942977140152 42-43 0.009721182308511914 44-45 0.003929839656632477 46-47 0.006057271651200433 48-49 0.0035161723243553734 50-51 7.386916647805406E-4 52-53 0.0 54-55 0.0 56-57 2.9547666591221623E-5 58-59 0.0016842169956996324 60-61 0.002777480659574833 62-63 0.0013000973300137514 64-65 0.0029547666591221624 66-67 0.002216074994341622 68-69 0.004964007987325233 70-71 8.864299977366488E-5 72-73 0.0 74-75 0.0 76-77 0.0963253930873825 78-79 0.015808001626303567 80-81 4.136673322771027E-4 82-83 0.0 84-85 0.0 86-87 0.1037123097351879 88-89 0.41121487595003137 90-91 0.41591295493803554 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.7728599954732976E-4 100-101 2.659289993209946E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1692181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.5768187100868 #Duplication Level Percentage of deduplicated Percentage of total 1 79.55716374376283 45.806483939633225 2 11.888361672157725 13.689880895155396 3 3.6163655128386476 6.246564645663627 4 1.5587979186451173 3.5900250066996224 5 0.8179276555521672 2.3546836170846723 6 0.5240568050552995 1.8104114195073766 7 0.3646136086651054 1.4695304151738915 8 0.2603568432797948 1.1992415012360982 9 0.18838497944747112 0.9761947028415426 >10 1.0714704708229847 11.432560837958027 >50 0.09126222272228927 3.6613291945841593 >100 0.05702620239497454 5.732783815921668 >500 0.0031849586420367856 1.178954561803325 >1k 0.0010274060135602534 0.851355446737253 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2495 0.1474428562901959 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2129 0.12581396434542166 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.9095333182443247E-5 0.0 6 0.0 0.0 0.0 1.7728599954732976E-4 1.1819066636488649E-4 7 0.0 0.0 0.0 1.7728599954732976E-4 1.1819066636488649E-4 8 0.0 0.0 0.0 1.7728599954732976E-4 1.1819066636488649E-4 9 0.0 0.0 0.0 2.3638133272977299E-4 1.7728599954732976E-4 10-11 0.0 0.0 0.0 2.3638133272977299E-4 1.7728599954732976E-4 12-13 0.0 0.0 0.0 2.3638133272977299E-4 2.0683366613855137E-4 14-15 0.0 0.0 0.0 2.3638133272977299E-4 2.3638133272977299E-4 16-17 0.0 0.0 0.0 2.3638133272977299E-4 2.3638133272977299E-4 18-19 0.0 0.0 0.0 2.3638133272977299E-4 2.3638133272977299E-4 20-21 0.0 0.0 0.0 2.3638133272977299E-4 2.3638133272977299E-4 22-23 0.0 0.0 0.0 2.659289993209946E-4 2.9547666591221624E-4 24-25 0.0 0.0 0.0 4.7276266545954597E-4 3.545719990946595E-4 26-27 0.0 0.0 0.0 6.500486650068758E-4 3.545719990946595E-4 28-29 0.0 0.0 0.0 0.0017728599954732974 3.545719990946595E-4 30-31 0.0 0.0 0.0 0.005289032319828671 3.545719990946595E-4 32-33 0.0 0.0 0.0 0.013680569631735612 3.545719990946595E-4 34-35 0.0 0.0 0.0 0.025736017600954036 3.545719990946595E-4 36-37 0.0 0.0 0.0 0.049846913539390886 3.545719990946595E-4 38-39 0.0 0.0 0.0 0.08979535877072252 3.545719990946595E-4 40-41 0.0 0.0 0.0 0.13228490332889922 3.545719990946595E-4 42-43 0.0 0.0 0.0 0.1754244965520828 4.1366733227710275E-4 44-45 0.0 0.0 0.0 0.2297035600801569 4.7276266545954597E-4 46-47 0.0 0.0 0.0 0.29724952590768955 4.7276266545954597E-4 48-49 0.0 0.0 0.0 0.372507432715531 4.7276266545954597E-4 50-51 0.0 0.0 0.0 0.4548863271718569 4.7276266545954597E-4 52-53 0.0 0.0 0.0 0.5396290349554805 5.318579986419893E-4 54-55 0.0 0.0 0.0 0.6337383530485214 5.909533318244325E-4 56-57 0.0 0.0 0.0 0.7500379687515697 5.909533318244325E-4 58-59 0.0 0.0 0.0 0.8869323080686995 5.909533318244325E-4 60-61 0.0 0.0 0.0 1.0358525476884566 5.909533318244325E-4 62-63 0.0 0.0 0.0 1.1895004139628091 7.09143998189319E-4 64-65 0.0 0.0 0.0 1.3512738885497475 7.09143998189319E-4 66-67 0.0 0.0 0.0 1.5430973400599581 7.09143998189319E-4 68-69 0.0 0.0 0.0 1.7626365028327347 7.09143998189319E-4 70-71 0.0 0.0 0.0 2.0141757885238043 7.09143998189319E-4 72-73 0.0 0.0 0.0 2.2710927495344766 7.682393313717623E-4 74-75 0.0 0.0 0.0 2.53554436552591 7.682393313717623E-4 76-77 0.0 0.0 0.0 2.824993307453517 7.682393313717623E-4 78-79 0.0 0.0 0.0 3.152026881285158 7.682393313717623E-4 80-81 0.0 0.0 0.0 3.5169405636867452 7.977869979629839E-4 82-83 0.0 0.0 0.0 3.8889161384036344 8.273346645542055E-4 84-85 0.0 0.0 0.0 4.264230599445331 8.273346645542055E-4 86-87 0.0 0.0 0.0 4.66740851008255 8.273346645542055E-4 88-89 0.0 0.0 0.0 5.116326208602981 8.273346645542055E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1835 0.0 32.32754 2 GTACCTG 2155 0.0 28.314985 1 GTACATG 7770 0.0 27.670002 1 TACATGG 7785 0.0 27.004978 2 ACATGGG 7835 0.0 25.924088 3 GTATCAA 7090 0.0 24.20527 1 GGTATCA 5400 0.0 23.835468 1 CCGTATA 100 0.0016627432 23.728416 9 ACCTGGG 2835 0.0 20.757132 3 TCAACGC 8245 0.0 20.548319 4 ATCAACG 8240 0.0 20.503193 3 CATGGGG 4105 0.0 20.3469 4 CAACGCA 8335 0.0 20.326439 5 CATGGGA 3840 0.0 20.268023 4 TATCAAC 8495 0.0 19.999466 2 AACGCAG 8575 0.0 19.81288 6 TATACCG 120 0.0047895364 19.77368 5 GTGTAGC 905 0.0 18.963024 1 GAGTACT 3390 0.0 18.898739 12-13 TGCACCG 455 0.0 18.77413 5 >>END_MODULE