FastQCFastQC Report
Fri 27 May 2016
SRR939101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133664
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.20963938089596112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.17500412436072407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22970.1076551884457909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41150.036.9160271
GTATCAA64300.034.3302351
ATCAACG74300.028.947723
TCAACGC74650.028.8119964
CAACGCA75050.028.6584345
TATCAAC76500.028.3010182
AACGCAG77600.027.7095436
ACGCAGA88600.024.2158497
CGCAGAG87950.024.0722718
GTATAGG5150.022.1227341
GAGTACT53500.021.68939812-13
GTACTTT54450.021.09451114-15
GCAGAGT100900.020.7944689
GTACATG61350.020.3505421
TACATGG58450.020.2629572
GTGTAAG9850.020.241741
ACATGGG57100.019.4975073
AGAGTAC82350.019.06580710-11
CAGAGTA94050.018.6579810-11
AGTACTT56950.018.62899812-13
Produced by FastQC (version 0.11.5)