##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939101_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2133664 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.997440084286936 33.0 31.0 34.0 30.0 34.0 2 32.106102460368646 34.0 31.0 34.0 30.0 34.0 3 32.16466603926391 34.0 31.0 34.0 30.0 34.0 4 35.59706589228669 37.0 35.0 37.0 33.0 37.0 5 35.63875286830541 37.0 35.0 37.0 35.0 37.0 6 35.71526913328434 37.0 36.0 37.0 35.0 37.0 7 35.637133119366496 37.0 36.0 37.0 33.0 37.0 8 35.62100452554854 37.0 35.0 37.0 33.0 37.0 9 37.300488736745805 39.0 38.0 39.0 34.0 39.0 10-11 37.20894691010393 39.0 37.0 39.0 34.0 39.0 12-13 37.1146598058551 39.0 37.0 39.0 34.0 39.0 14-15 38.42271182341737 40.0 38.0 41.0 34.0 41.0 16-17 38.342051747604124 40.0 38.0 41.0 33.5 41.0 18-19 38.25858335707966 40.0 38.0 41.0 33.5 41.0 20-21 38.14354954669526 40.0 38.0 41.0 33.0 41.0 22-23 38.063636776924575 40.0 38.0 41.0 33.0 41.0 24-25 38.110506621473675 40.0 38.0 41.0 33.0 41.0 26-27 38.03276335917933 40.0 38.0 41.0 33.0 41.0 28-29 37.90506963608141 40.0 38.0 41.0 33.0 41.0 30-31 37.74585103371477 40.0 38.0 41.0 32.5 41.0 32-33 37.555107786418105 40.0 37.5 41.0 32.0 41.0 34-35 37.256183729022 40.0 37.0 41.0 31.0 41.0 36-37 37.009211619074044 40.0 37.0 41.0 30.5 41.0 38-39 36.7724899515575 40.0 36.5 41.0 30.0 41.0 40-41 36.63230480525519 40.0 36.0 41.0 30.0 41.0 42-43 36.45368928753543 40.0 36.0 41.0 30.0 41.0 44-45 36.21277108298214 39.5 36.0 41.0 29.5 41.0 46-47 35.90219734691122 39.0 35.0 41.0 28.0 41.0 48-49 35.91654848186032 39.0 36.0 41.0 28.0 41.0 50-51 34.93674706982918 38.0 34.0 40.0 25.5 40.5 52-53 35.10034382170764 38.5 35.0 40.0 26.5 40.5 54-55 35.61007825037119 39.0 35.0 41.0 27.0 41.0 56-57 35.49370402275147 39.0 35.0 41.0 27.0 41.0 58-59 35.297684874469454 39.0 35.0 41.0 26.0 41.0 60-61 35.01031441689038 38.5 35.0 41.0 26.0 41.0 62-63 34.64608813758868 38.0 34.5 40.0 26.0 41.0 64-65 34.28123359629257 37.5 34.0 40.0 24.5 41.0 66-67 33.8927251432278 37.0 34.0 40.0 23.0 41.0 68-69 33.48607536144398 36.5 34.0 39.0 22.5 41.0 70-71 33.08601237120746 36.0 33.0 39.0 22.0 41.0 72-73 32.6305941329094 35.0 33.0 38.5 20.5 40.0 74-75 32.1721020741785 35.0 32.0 37.5 19.5 39.5 76-77 31.72961300373442 35.0 32.0 37.0 18.0 39.0 78-79 31.301950307077405 35.0 31.5 36.5 15.0 39.0 80-81 30.877516094380372 35.0 31.0 36.0 9.5 38.5 82-83 30.44778559323305 35.0 31.0 35.5 7.0 37.0 84-85 30.06671340942154 34.0 31.0 35.0 3.5 37.0 86-87 29.67845897948318 34.0 30.0 35.0 2.0 36.0 88-89 29.271748503981883 34.0 30.0 35.0 2.0 36.0 90-91 28.887899641180617 34.0 29.0 35.0 2.0 36.0 92-93 28.47800403437467 34.0 29.0 35.0 2.0 35.0 94-95 28.02392691632797 34.0 29.0 35.0 2.0 35.0 96-97 27.46576405657123 34.0 27.0 35.0 2.0 35.0 98-99 26.759023445116007 34.0 25.5 35.0 2.0 35.0 100-101 24.15104744702071 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.171532932968276 1101 2 0.2228229979034566 1101 3 0.21850192384198408 1101 4 0.15389936909415525 1101 5 0.2597065488169932 1101 6 0.2694286995932842 1101 7 0.20619099218381365 1101 8 0.31211195153790783 1101 9 0.3880250887684582 1101 10-11 0.3058673444470159 1101 12-13 0.28857574576890954 1101 14-15 0.3087816261577956 1101 16-17 0.4237398556544676 1101 18-19 0.4074720419254092 1101 20-21 0.4724737554080036 1101 22-23 0.4573261261390016 1101 24-25 0.35648687042124294 1101 26-27 0.32483007340200487 1101 28-29 0.35684671255422984 1101 30-31 0.38538989112635846 1101 32-33 0.4445998865637435 1101 34-35 0.4076344221279271 1101 36-37 0.32886852897973995 1101 38-39 0.3596408509136424 1101 40-41 0.2015240049909508 1101 42-43 0.10322764498527448 1101 44-45 0.12737425548314718 1101 46-47 0.04674426050242175 1101 48-49 0.25884404927300153 1101 50-51 0.07765725111465827 1101 52-53 0.21301657002008056 1101 54-55 0.1452297637181701 1101 56-57 0.07313592686633541 1101 58-59 0.052199798502961414 1101 60-61 0.06328442802586665 1101 62-63 0.15275943745398735 1101 64-65 0.08345778557197292 1101 66-67 0.04953085249271538 1101 68-69 1.5162359625264799E-4 1101 70-71 -0.0010979062381366589 1101 72-73 0.014757751109122808 1101 74-75 -0.15982705089536609 1101 76-77 -0.19038122677030245 1101 78-79 -0.2045546191851102 1101 80-81 -0.26220438470231855 1101 82-83 -0.13377465957864842 1101 84-85 -0.22097226113379875 1101 86-87 -0.4312089819861775 1101 88-89 -0.27669054699950735 1101 90-91 -0.43567424688651 1101 92-93 -0.4200080155998336 1101 94-95 -0.7219608523466228 1101 96-97 -0.8805490558081921 1101 98-99 -0.8271748917554049 1101 100-101 -0.9528970829987671 1102 1 0.1075557002080032 1102 2 0.20267830654612595 1102 3 0.3120185571826468 1102 4 0.29202426662896386 1102 5 0.2749867184222765 1102 6 0.33011471542442195 1102 7 0.3257618784779268 1102 8 0.4179521520824281 1102 9 0.3655868586383022 1102 10-11 0.2439443198007183 1102 12-13 0.30117703742582336 1102 14-15 0.3791637565089303 1102 16-17 0.3779629628334078 1102 18-19 0.46365841717290124 1102 20-21 0.4667760602967661 1102 22-23 0.47327132109960957 1102 24-25 0.3606866470646466 1102 26-27 0.402789513301002 1102 28-29 0.4249196103188453 1102 30-31 0.38246005228220525 1102 32-33 0.4604325624850887 1102 34-35 0.18396268626602108 1102 36-37 0.33415440160482746 1102 38-39 0.2907546773036316 1102 40-41 0.3213772398082355 1102 42-43 0.32065002015990274 1102 44-45 0.2780662545807502 1102 46-47 0.2774890020239127 1102 48-49 0.2729082285636153 1102 50-51 0.3264731388590505 1102 52-53 0.3657085495004182 1102 54-55 0.4299404860960081 1102 56-57 0.3883432323366094 1102 58-59 0.3524422118752213 1102 60-61 0.36822167378959136 1102 62-63 0.3596857339801929 1102 64-65 0.3426971680764552 1102 66-67 0.3038643768608651 1102 68-69 0.4339045329070288 1102 70-71 0.27424585133729096 1102 72-73 0.1722853869341776 1102 74-75 -0.05495510260648473 1102 76-77 -0.06748675505630075 1102 78-79 0.07678543633175394 1102 80-81 0.08661458575548053 1102 82-83 -0.1522252597969569 1102 84-85 -0.11129156018082043 1102 86-87 -0.18898001204683368 1102 88-89 0.028080652610388057 1102 90-91 -0.18631860404968492 1102 92-93 -0.2931641676448642 1102 94-95 -0.44805966452110013 1102 96-97 -0.4606326253661166 1102 98-99 -0.5577898790672364 1102 100-101 -0.45538160987933196 1103 1 0.34700815513611616 1103 2 0.3120495390736622 1103 3 0.4165371129532929 1103 4 0.3556353911355359 1103 5 0.35346588236201626 1103 6 0.34253574920163743 1103 7 0.3556591621087577 1103 8 0.3813090690757477 1103 9 0.509319506736567 1103 10-11 0.47144557706010204 1103 12-13 0.42504843184703844 1103 14-15 0.5294940884943031 1103 16-17 0.4733520932232622 1103 18-19 0.4752327814818855 1103 20-21 0.6242793153929753 1103 22-23 0.5564956919532378 1103 24-25 0.5932438093096621 1103 26-27 0.4717169504897072 1103 28-29 0.5224664044796512 1103 30-31 0.5060310822704324 1103 32-33 0.5240643542691572 1103 34-35 0.5931238014723093 1103 36-37 0.3939048786744834 1103 38-39 0.5654172839984497 1103 40-41 0.43772505631036296 1103 42-43 0.4269218499717553 1103 44-45 0.3702795133281782 1103 46-47 0.536664239869836 1103 48-49 0.5067488287413582 1103 50-51 0.585585685963224 1103 52-53 0.41898879089421825 1103 54-55 0.5665029667204422 1103 56-57 0.4284088164972033 1103 58-59 0.3988795176889184 1103 60-61 0.502422573505072 1103 62-63 0.31028818511929757 1103 64-65 0.19643760084924367 1103 66-67 0.16949538328838543 1103 68-69 0.07868845652077283 1103 70-71 0.17500502933076945 1103 72-73 0.10442162562745239 1103 74-75 0.03040075346659421 1103 76-77 0.0988407386229575 1103 78-79 0.24603114959394645 1103 80-81 0.2545224372542023 1103 82-83 0.3241520420210726 1103 84-85 0.2899519210154011 1103 86-87 0.11843265148299764 1103 88-89 0.05130447233661428 1103 90-91 0.10474899861501541 1103 92-93 0.11201160755026862 1103 94-95 0.03614915765075821 1103 96-97 0.059125980832689606 1103 98-99 -0.1957777129298819 1103 100-101 -0.23273440719534122 1104 1 0.25990997503443936 1104 2 0.2396374060605666 1104 3 0.21720494094333276 1104 4 0.12803853931423248 1104 5 0.21969537648901394 1104 6 0.23861205860715273 1104 7 0.11500613898018486 1104 8 0.1929670928681304 1104 9 0.28148046669236493 1104 10-11 0.2854074733161056 1104 12-13 0.3313032176123798 1104 14-15 0.41886315196612145 1104 16-17 0.18286408261311493 1104 18-19 0.2903809440832532 1104 20-21 0.32733523190642444 1104 22-23 0.25024828179822833 1104 24-25 0.37357935934915076 1104 26-27 0.3459275255056937 1104 28-29 0.34802463760525626 1104 30-31 0.2770466769927111 1104 32-33 0.33666404234789127 1104 34-35 0.29434439793121925 1104 36-37 0.3679487648969726 1104 38-39 0.4423957244189367 1104 40-41 0.32384040280984294 1104 42-43 0.19863021565720373 1104 44-45 0.07541079248698423 1104 46-47 0.09882105607364622 1104 48-49 0.15379798114962995 1104 50-51 0.16952469100679934 1104 52-53 0.12178575643402922 1104 54-55 0.20376924368667204 1104 56-57 0.09711906363379086 1104 58-59 0.10267916392782439 1104 60-61 0.21115247999573938 1104 62-63 0.2772253355321297 1104 64-65 0.29106574269162167 1104 66-67 0.10061670403222678 1104 68-69 0.1025122143003685 1104 70-71 0.08414823820402972 1104 72-73 0.053490488348572285 1104 74-75 0.07749831669904239 1104 76-77 0.2452736923135106 1104 78-79 0.05905272258365457 1104 80-81 0.049076420687239164 1104 82-83 0.007395996367385749 1104 84-85 0.14771783523571358 1104 86-87 0.21189705611702792 1104 88-89 0.2912195321259965 1104 90-91 0.05986814154227815 1104 92-93 -0.19651795054338805 1104 94-95 -0.21371157741598168 1104 96-97 -0.4016300058161164 1104 98-99 -0.42077982503647604 1104 100-101 -0.33234081780763347 1105 1 0.1570580841361604 1105 2 0.17722946015574337 1105 3 0.2036301719715965 1105 4 0.17777690680460267 1105 5 0.20753709171699342 1105 6 0.274942484054435 1105 7 0.11587039515219999 1105 8 0.1839383577020186 1105 9 0.29803803936055573 1105 10-11 0.26440774749925566 1105 12-13 0.3378029028810161 1105 14-15 0.35205244260411916 1105 16-17 0.2385398668348344 1105 18-19 0.4237208423651637 1105 20-21 0.408176394906377 1105 22-23 0.4090474368507273 1105 24-25 0.3633106212481252 1105 26-27 0.4305991429159377 1105 28-29 0.4181579832961404 1105 30-31 0.37228091049635026 1105 32-33 0.3791164754336904 1105 34-35 0.37454798857191207 1105 36-37 0.3289467915506705 1105 38-39 0.3054887420168697 1105 40-41 0.29733229896007884 1105 42-43 0.11190454168799846 1105 44-45 0.11061861172691323 1105 46-47 0.26024734907888103 1105 48-49 0.2476068484642937 1105 50-51 0.22547195516101937 1105 52-53 0.22217888100199445 1105 54-55 0.28010878282728413 1105 56-57 0.2625928405259472 1105 58-59 0.2953681282492653 1105 60-61 0.3336219819478501 1105 62-63 0.30831654583833057 1105 64-65 0.32267944567017537 1105 66-67 0.3251259741257968 1105 68-69 0.455110582331379 1105 70-71 0.3022003021080124 1105 72-73 0.3999568082314866 1105 74-75 0.26298089834576643 1105 76-77 0.3831827948078086 1105 78-79 0.29384192980818824 1105 80-81 0.291986855479923 1105 82-83 0.1348988830138964 1105 84-85 0.11247101247773728 1105 86-87 0.09521861116025931 1105 88-89 0.08114735630894998 1105 90-91 0.14975773455407904 1105 92-93 0.14972207170020013 1105 94-95 0.024521524599460776 1105 96-97 0.09159226403416199 1105 98-99 -0.022067327305244078 1105 100-101 -0.1484407749423866 1106 1 0.1414241929407538 1106 2 0.1816862169423672 1106 3 0.012712898385871085 1106 4 0.11821324998985716 1106 5 0.14142448044849942 1106 6 0.07295638210185729 1106 7 0.08413967196992189 1106 8 0.035026145766003935 1106 9 0.1307071693142987 1106 10-11 0.2218246852380119 1106 12-13 0.11198554223295076 1106 14-15 0.23962082460763412 1106 16-17 0.221712276054852 1106 18-19 0.19494397614277403 1106 20-21 0.27200229969775336 1106 22-23 0.33027038643438544 1106 24-25 0.21315252144202645 1106 26-27 0.24165335856284287 1106 28-29 0.21446170192637481 1106 30-31 0.16049102421769135 1106 32-33 0.10699828186058369 1106 34-35 0.05361864414273754 1106 36-37 0.20538507000033945 1106 38-39 0.1845547313409881 1106 40-41 0.3548090383342313 1106 42-43 0.14464661244549148 1106 44-45 0.13205706823073626 1106 46-47 0.027606707789232132 1106 48-49 0.057511202686448826 1106 50-51 0.12150687342188604 1106 52-53 0.02652733174668498 1106 54-55 0.05986463988786994 1106 56-57 0.06722458156399114 1106 58-59 0.010413949563947256 1106 60-61 0.0544350385973118 1106 62-63 0.03687679997685933 1106 64-65 0.03523238608097756 1106 66-67 -0.03004613056022265 1106 68-69 -0.011119053492627984 1106 70-71 -0.054557221293826785 1106 72-73 -0.08036108596505898 1106 74-75 -0.1255419767007453 1106 76-77 0.07365738893643936 1106 78-79 0.00763817937379585 1106 80-81 -0.014332102129682056 1106 82-83 0.138792129112975 1106 84-85 0.05918038758134614 1106 86-87 0.09540280510912069 1106 88-89 0.11505144529181521 1106 90-91 0.2771941600126304 1106 92-93 0.2496113097389525 1106 94-95 0.17048380904649107 1106 96-97 0.34753270307886197 1106 98-99 0.12528169198477457 1106 100-101 -0.05508931692448371 1107 1 0.009554248827321032 1107 2 -0.02390258343638152 1107 3 -0.0029970458041574943 1107 4 0.03007815332588848 1107 5 0.1376079944256432 1107 6 0.02028360921712391 1107 7 0.03359044025764035 1107 8 0.057960246816598726 1107 9 0.1660191793135155 1107 10-11 0.1306337668710853 1107 12-13 0.16621087934391454 1107 14-15 0.237838720707785 1107 16-17 0.12588353132091612 1107 18-19 0.19714805965117677 1107 20-21 0.10483291162562836 1107 22-23 0.13724176243018604 1107 24-25 0.22113459006121872 1107 26-27 0.16965317761186327 1107 28-29 0.03546970422033979 1107 30-31 -0.015462385212394736 1107 32-33 0.0638996350915022 1107 34-35 0.03478382486513709 1107 36-37 -5.263784285105544E-4 1107 38-39 0.09565920971028419 1107 40-41 -0.0214043325551998 1107 42-43 0.19655632747880958 1107 44-45 0.06281064530377023 1107 46-47 0.24365236871150842 1107 48-49 0.2802927400373889 1107 50-51 0.038942123008581575 1107 52-53 0.12181047096860453 1107 54-55 0.14939839670075372 1107 56-57 0.2337826616836267 1107 58-59 0.2292612391408042 1107 60-61 0.07054697640761987 1107 62-63 0.1053955893190377 1107 64-65 0.03934507163052814 1107 66-67 0.03673866486583677 1107 68-69 0.12715574163902943 1107 70-71 0.009540954667599522 1107 72-73 0.0725837835838874 1107 74-75 0.024023465682525114 1107 76-77 0.04268927963246938 1107 78-79 0.06799311137715591 1107 80-81 0.04026913910789531 1107 82-83 0.09550080305581687 1107 84-85 0.12817967837795763 1107 86-87 0.04159346377788253 1107 88-89 5.767309179169899E-4 1107 90-91 0.0558884742024226 1107 92-93 0.1820328192579055 1107 94-95 0.13087301642134364 1107 96-97 0.07652748924913055 1107 98-99 0.040659102839487815 1107 100-101 -0.12030228019550293 1108 1 -0.1287473385113671 1108 2 0.10081114163216398 1108 3 0.05220615738969059 1108 4 -0.0033349637809507726 1108 5 0.08529968000589605 1108 6 0.12684496753586672 1108 7 0.175268891392804 1108 8 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2316 82-83 -1.0182030883323812 2316 84-85 -0.9194670416127124 2316 86-87 -0.8919040462294845 2316 88-89 -1.085365474274976 2316 90-91 -1.159506096665762 2316 92-93 -1.2630687673376926 2316 94-95 -1.0790947923122225 2316 96-97 -0.9532610072036292 2316 98-99 -0.8337443744005952 2316 100-101 -0.6283298633350078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8553.0 3 2422.0 4 2321.0 5 2608.0 6 2932.0 7 3392.0 8 4014.0 9 4779.0 10 5780.0 11 6970.0 12 8528.0 13 12321.0 14 10743.0 15 10676.0 16 12222.0 17 15306.0 18 14572.0 19 11323.0 20 11516.0 21 12210.0 22 13062.0 23 14482.0 24 16048.0 25 18352.0 26 20734.0 27 24475.0 28 28380.0 29 33358.0 30 40734.0 31 49663.0 32 62696.0 33 81173.0 34 112871.0 35 166504.0 36 273186.0 37 454975.0 38 469102.0 39 90650.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94448810251071 18.80164016379064 14.519653889510447 23.7342178441882 2 15.21050890483754 19.677403461519514 41.20324989618257 23.908837737460377 3 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21.980733730375913 22-23 22.620532383620265 26.262405157730264 28.513914891819987 22.603147566829488 24-25 22.49022690475632 26.444892883121756 28.63554450102072 22.429335711101203 26-27 22.349141855274542 26.613491352852066 28.51324994978591 22.52411684208748 28-29 22.300680928762336 26.97961718668795 28.436618439394152 22.283083445155565 30-31 22.480850035543273 26.634368819517597 28.38976072222053 22.495020422718603 32-33 22.248063906793522 26.560932627803734 28.467349863044806 22.72365360235794 34-35 22.66850065541585 26.81383250745058 28.231999576771827 22.28566726036174 36-37 22.703322412726404 26.500782718074774 28.292873862397528 22.503021006801298 38-39 22.372858609355724 26.80081653026436 28.35212985374031 22.474195006639604 40-41 22.729617051667645 26.639487911385867 28.310983715109394 22.31991132183709 42-43 22.518463470068035 26.67066029569809 28.110839217813844 22.700037016420033 44-45 22.56784589147389 26.867354517713803 28.09795542736518 22.466844163447124 46-47 22.762187729325063 26.946678360380545 27.729339195262725 22.561794715031667 48-49 22.64641345679152 26.96240950733551 27.759131232735264 22.632045803137707 50-51 22.629478600101503 27.14480007253604 27.73533294160731 22.490388385755146 52-53 22.80321375563531 27.05415782108235 27.64662159520565 22.4960068280767 54-55 22.631050476050532 27.001526865677267 27.745367596915 22.622055061357205 56-57 22.532495203117094 27.26164494826429 27.578345163682595 22.627514684936024 58-59 22.6656338128289 26.97403248217044 27.67593998996804 22.684393715032613 60-61 22.490999158148945 27.079134469824417 27.81814229023956 22.611724081787077 62-63 22.442422408728792 27.097392452959024 27.922709522626644 22.537475615685544 64-65 22.361645239759646 27.058009982683867 27.83045265008413 22.749892127472354 66-67 22.370534172703387 27.16908583066025 27.603885235004615 22.856494761631748 68-69 22.583580811785865 26.909683204022606 27.847015204906505 22.659720779285028 70-71 22.518127694537924 26.899939272046623 27.635341124790997 22.946591908624452 72-73 22.50710512666746 26.97887140486244 27.628378674397467 22.885644794072633 74-75 22.415633295306492 27.210030417546964 27.472064640761772 22.90227164638477 76-77 22.51694791116671 27.038824411020933 27.488138934955725 22.956088742856632 78-79 22.52799349775273 27.114201604041487 27.545856077032415 22.811948821173367 80-81 22.589622100627654 27.103637955745153 27.428402034996722 22.878337908630463 82-83 22.588948019979764 27.171619337067703 27.463789565846863 22.77564307710567 84-85 22.552550063322407 27.16773868120782 27.39863351702876 22.88107773844101 86-87 22.593519921024274 27.124592122020513 27.4291347334946 22.852753223460613 88-89 22.61144966647181 27.08618221506433 27.334379382253317 22.967988736210547 90-91 22.538016109587026 27.106309423359328 27.39458971528611 22.961084751767537 92-93 22.67366581815381 26.95354833755627 27.46404576928757 22.90874007500235 94-95 22.590574226640207 26.85526850439606 27.53588401222536 23.018273256738375 96-97 22.683174450623873 26.660447980929362 27.579777022427315 23.076600546019453 98-99 22.456739450257846 26.793417301958705 27.420284843862547 23.329558403920903 100-101 22.80309901088733 26.27364830413772 27.796682578414362 23.12657010656059 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1852.0 1 1044.0 2 248.5 3 249.5 4 216.5 5 236.5 6 370.0 7 709.5 8 1195.5 9 1487.0 10 1433.0 11 1411.0 12 1562.0 13 1695.0 14 1908.0 15 2156.0 16 2320.5 17 2441.5 18 2611.0 19 2805.0 20 3091.5 21 3633.5 22 4320.5 23 5175.5 24 6554.0 25 8509.5 26 11052.5 27 14165.5 28 17031.0 29 20074.0 30 23576.5 31 27550.0 32 32911.5 33 37173.5 34 40418.5 35 44609.0 36 49411.0 37 53593.0 38 57173.0 39 60257.5 40 63198.5 41 66991.0 42 69266.5 43 71506.0 44 75531.0 45 78702.5 46 80336.0 47 82175.5 48 84446.0 49 84147.0 50 80980.5 51 77979.0 52 76413.5 53 75400.5 54 73435.0 55 67905.5 56 61461.0 57 55744.0 58 50676.0 59 45239.0 60 37353.5 61 30512.0 62 24615.0 63 18286.0 64 13268.5 65 9918.5 66 7428.5 67 5785.5 68 4454.0 69 3495.5 70 2699.0 71 1900.0 72 1339.5 73 1045.5 74 793.5 75 550.5 76 406.0 77 284.0 78 185.5 79 125.5 80 88.0 81 58.5 82 45.5 83 38.5 84 27.5 85 21.0 86 13.0 87 10.5 88 12.5 89 13.5 90 12.0 91 11.0 92 7.5 93 3.5 94 2.5 95 4.5 96 4.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34171265953777163 2 0.1180598257270123 3 0.11238882973139162 4 0.11984079967605021 5 0.13690065539841326 6 0.08684591388334807 7 0.09542270948003059 8 0.10259347301168319 9 0.08895496198089296 10-11 0.08780670246111852 12-13 0.09258721148222025 14-15 0.0938057726052462 16-17 0.08218257420099885 18-19 0.09821133974233995 20-21 0.10885031570106633 22-23 0.11688813233948739 24-25 0.13188580769980654 26-27 0.1306672465767806 28-29 0.1254883618039204 30-31 0.11524776159695248 32-33 0.10826446900730387 34-35 0.3351746104353825 36-37 0.4974307107398353 38-39 0.47073953537201735 40-41 0.44892260449630306 42-43 0.4818940564212547 44-45 0.48897108448190524 46-47 0.49614184801355793 48-49 0.5080462528308113 50-51 0.49593094320380343 52-53 0.4998443991181367 54-55 0.4855497397903325 56-57 0.4634750363693627 58-59 0.4430172698231774 60-61 0.4023595092760622 62-63 0.47413744619583964 64-65 0.5056091305847593 66-67 0.5137172488264319 68-69 0.527590098534727 70-71 0.5194351125575536 72-73 0.510905184696372 74-75 0.5174432337987611 76-77 0.494032799916013 78-79 0.4729188850728137 80-81 0.4744186526088457 82-83 0.4881508991106379 84-85 0.4875650524168754 86-87 0.4911738680504522 88-89 0.5040624953132264 90-91 0.5191539061445476 92-93 0.5122877828936514 94-95 0.5167870855017472 96-97 0.5169042548404997 98-99 0.5102490363993581 100-101 0.4967745624428214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2133664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.83330064705087 #Duplication Level Percentage of deduplicated Percentage of total 1 80.0284467900058 51.084799042629534 2 12.132464956050013 15.489105662586985 3 3.5639972964940405 6.8250513279714164 4 1.4632161816075495 3.7360767372873798 5 0.7831828099376578 2.499657188417631 6 0.45109194207412345 1.7276812534727748 7 0.2772595371531124 1.2388873974649757 8 0.22505372627418466 1.1492737736799297 9 0.15133050986485863 0.8693933339947517 >10 0.8542367649033915 9.729659426163257 >50 0.04882126402471257 2.130222705563316 >100 0.01906020267917779 2.268596618708724 >500 0.0012498528732587952 0.5061948107838469 >1k 5.881660580038943E-4 0.745400721275465 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4473 0.20963938089596112 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.17500412436072407 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2297 0.1076551884457909 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 4.686773550099734E-5 0.0 0.0 0.0 0.0 16-17 7.030160325149601E-5 0.0 0.0 7.030160325149601E-5 0.0 18-19 9.373547100199469E-5 0.0 0.0 1.4060320650299203E-4 0.0 20-21 9.373547100199469E-5 0.0 0.0 1.4060320650299203E-4 0.0 22-23 9.373547100199469E-5 0.0 0.0 1.8747094200398937E-4 0.0 24-25 2.3433867750498671E-4 0.0 0.0 3.515080162574801E-4 0.0 26-27 2.3433867750498671E-4 0.0 0.0 4.921112227604721E-4 0.0 28-29 2.577725452554854E-4 0.0 0.0 0.0016638046102854058 0.0 30-31 2.8120641300598406E-4 0.0 0.0 0.0052960541116127 2.343386775049867E-5 32-33 2.8120641300598406E-4 0.0 0.0 0.012162177362508811 9.373547100199469E-5 34-35 3.7494188400797874E-4 0.0 0.0 0.023363566147247175 9.373547100199469E-5 36-37 3.7494188400797874E-4 0.0 0.0 0.04546170343596742 1.4060320650299203E-4 38-39 3.7494188400797874E-4 0.0 0.0 0.07503524453709676 1.640370742534907E-4 40-41 3.7494188400797874E-4 0.0 0.0 0.10699904014877694 1.8747094200398937E-4 42-43 3.7494188400797874E-4 0.0 0.0 0.14156399508076248 2.3433867750498671E-4 44-45 4.6867735500997343E-4 0.0 0.0 0.18479948108043254 2.8120641300598406E-4 46-47 5.155450905109707E-4 0.0 0.0 0.23986907029410443 3.0464028075648274E-4 48-49 5.155450905109707E-4 0.0 0.0 0.3002581474871395 3.7494188400797874E-4 50-51 5.155450905109707E-4 0.0 0.0 0.36507622568501885 3.7494188400797874E-4 52-53 5.155450905109707E-4 0.0 0.0 0.43071448925416556 3.7494188400797874E-4 54-55 5.155450905109707E-4 0.0 0.0 0.5071791997240428 3.7494188400797874E-4 56-57 5.155450905109707E-4 0.0 0.0 0.6015473851553009 3.7494188400797874E-4 58-59 5.155450905109707E-4 0.0 0.0 0.7065076788097845 3.7494188400797874E-4 60-61 5.155450905109707E-4 0.0 0.0 0.8191777149541821 4.6867735500997343E-4 62-63 5.155450905109707E-4 0.0 0.0 0.935808074748414 5.624128260119681E-4 64-65 5.624128260119681E-4 0.0 0.0 1.0634757862531308 5.624128260119681E-4 66-67 5.624128260119681E-4 0.0 0.0 1.2163349055896338 6.092805615129655E-4 68-69 5.624128260119681E-4 0.0 0.0 1.3900032994885794 6.561482970139629E-4 70-71 5.624128260119681E-4 0.0 0.0 1.5838716873884549 6.561482970139629E-4 72-73 5.624128260119681E-4 0.0 0.0 1.78148949412841 7.498837680159575E-4 74-75 6.092805615129655E-4 0.0 0.0 1.9995650674145509 7.498837680159575E-4 76-77 6.561482970139629E-4 0.0 0.0 2.2364814703720923 7.498837680159575E-4 78-79 6.795821647644616E-4 0.0 0.0 2.503791599802031 7.967515035169549E-4 80-81 9.139208422694482E-4 0.0 0.0 2.7961759677250027 8.436192390179522E-4 82-83 9.842224455209442E-4 0.0 0.0 3.10055847593623 8.904869745189496E-4 84-85 9.842224455209442E-4 0.0 0.0 3.421110352895301 8.904869745189496E-4 86-87 9.842224455209442E-4 0.0 0.0 3.76129981102929 9.139208422694482E-4 88-89 9.842224455209442E-4 0.0 0.0 4.127711767176088 9.373547100199469E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4115 0.0 36.916027 1 GTATCAA 6430 0.0 34.330235 1 ATCAACG 7430 0.0 28.94772 3 TCAACGC 7465 0.0 28.811996 4 CAACGCA 7505 0.0 28.658434 5 TATCAAC 7650 0.0 28.301018 2 AACGCAG 7760 0.0 27.709543 6 ACGCAGA 8860 0.0 24.215849 7 CGCAGAG 8795 0.0 24.072271 8 GTATAGG 515 0.0 22.122734 1 GAGTACT 5350 0.0 21.689398 12-13 GTACTTT 5445 0.0 21.094511 14-15 GCAGAGT 10090 0.0 20.794468 9 GTACATG 6135 0.0 20.350542 1 TACATGG 5845 0.0 20.262957 2 GTGTAAG 985 0.0 20.24174 1 ACATGGG 5710 0.0 19.497507 3 AGAGTAC 8235 0.0 19.065807 10-11 CAGAGTA 9405 0.0 18.65798 10-11 AGTACTT 5695 0.0 18.628998 12-13 >>END_MODULE