FastQCFastQC Report
Fri 27 May 2016
SRR939101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133664
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47130.2208876374162005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40210.18845516444951033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.1149196874484455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.035.8624761
GTATCAA67250.032.2060471
ATCAACG78150.027.0780563
TCAACGC79900.026.4255964
CAACGCA80600.026.2549635
TATCAAC81700.025.9595432
AACGCAG83950.025.3203036
ACGCAGA95100.022.1520567
CGCAGAG95350.022.0442148
GAGTACT59550.020.079412-13
TACATGG63250.019.879112
GTACATG65950.019.7045331
GTACTTT60600.019.4574414-15
GCAGAGT108500.019.1105619
GTGCGAA1250.005959057719.0404971
ACATGGG62650.019.0092163
AGAGTAC88650.018.49273910-11
GTACCTG16100.017.7395951
GAGTACA51600.017.5275921
AGTACTT64050.017.33519212-13
Produced by FastQC (version 0.11.5)